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Original

Standardization Of Protein Immunoprocedures Choriogonadotropin (Cg)

Pages 42-78 | Published online: 29 Mar 2011

References

  • Wide L, Gemzell C A. An immunological pregnancy test. Acta Endocrinol 1960; 35: 261–267
  • Chard T. Pregnancy tests: a review. Hum Re-prod 1992; 7: 701–710
  • Vaitukaitis J L, Braunstein G D, Ross G T. A radioimmunoassay which specifically measures human chorionic gonadotropin in the presence of human luteinizing hormone. Am J Obstet Gynecol 1972; 113: 751–758
  • Chen H-C, Hodgen G D, Matsuura S, Lin L J, Gross E, Reichert L EJ, et al. Evidence for a go nadotropin from nonpregnant subjects that has physiological, immunological, and biological similarities to human chorionic gonadotropin. Proc Natl Acad Sci 1976; 73: 2885–2889
  • Hussa R O. The clinical marker hCG. Praeger, New York 1987
  • Pierce J G, Parsons T F. Glycoprotein hormones: Structure and function. Ann Rev Biochem 1981; 50: 465–495
  • Hussa R. Biosynthesis of human chorionic go nadotropin. Endocrinol Rev 1980; 1: 268–294
  • Gordon W L, Ward D N. Structural aspects of luteinizing hormone actions. Luteinizing hormone action and receptors, M A Ascoli. CRC Press, Boca Raton 1985; 173–197
  • Mise T, Bahl O P. Assignment of disulfide bonds in the alpha subunit of human chorionic gona dotropin. J Biol Chem 1980; 255: 8516–22
  • Mise T, Bahl O P. Assignment of disulfide bonds in the beta subunit of human chorionic gonado tropin. J Biol Chem 1981; 256: 6587–92
  • Birken S, Krichevsky A, O'Connor J, Lustbader J W, Canfield R. Chemistry and imrnunochem-istry of hCG, its subunits, and its fragments. Glycoprotein hormones, Serono Symposia, W W Chin, I Boime. Raven Press, Norwell 1990; 45–61
  • Cole L A, Kardana A, Birken S. The isomers, subunits and fragments of hCG. Structure-function relationship of gonadotropins, D Belief, J-M Bidart. Raven Press, New York 1989; 59–80
  • Nilson J H, Bokar J A, Clay C M, Farmerie T A, Fenstermaker R A, Hamernik D L, Keri R A. Different combinations of regulatory elements may explain why placenta-specific expression of the glycoprotein hormone a-subunit gene occurs only in primates and horses. Biol Reprod 1991; 44: 231–237
  • Cole L A, Kroll T G, Ruddon R W, Hussa R O. Differential occurrence of free beta and free alpha subunits of human chorionic gonadotropin (hCG) in pregnancy sera. J Clin Endocri nol Metab 1984; 58: 1200–1202
  • Ozturk M, Bellet D, Manil L, Hennen G, Frydman R, Wands J. Physiological studies of human chorionic gonadotropin (hCG), cthCG and BhCG as measured by specific monoclonal immunoradiometric assays. Endocrinol 1987; 120: 549–558
  • Alfthan H, Schroder J, Fraser R, Koskimies A, Halila H, Stenman U-H. Choriogonadotropin and its fj subunit separated by hydrophobic-in-teraction chromatography and quantified in serum during pregnancy by time-resolved im-munofluorometric assays. Clin Chem 1988; 34: 1758–1762
  • Schroeder H R, Halter C M. Specificity of human fi-choriogonadotropin assays for the hormone and for an immunoreactive fragment present in the urine during normal pregnancy. Clin Chem 1983; 29: 667–671
  • Kato Y, Braunstein G D. β-core fragment is a major form of immunoreactive urinary cho rionic gonadotropin in human pregnancy. J Clin Endocrinol Metab 1988; 66: 1197–201
  • Blithe D L, Wehmann R E, Nisulaβ-core B C. Chemical and clinical properties. Trends Endo crinol Metab 1990; 1: 394–398
  • Ami S, Rosa C, Birken S, Canfield R, Nisula B. Carboxyterminal peptide fragments of the beta subunit are urinary products of the metabolism of desialylated human choriogonadotropin. J Clin Invest 1985; 76: 350–6
  • Alfthan H, Schroder J, Stenman U-H. Demon stration of the core fihCG fragment in serum of pregnant women and cancer patients. Interna tional Society for Oncodevelopmental Biology and Medicine, Freiburg 1989; 18–229
  • Alfthan H, Haglund C, Dabek J, Stenman U-H. Concentrations of hCG, BhCG and cBhCG in serum and urine of nonpregnant women and men. Clin Chem 1992; 38: 1981–7
  • Bagshawe K D. Choriocarcinoma. A model for tumor markers. Acta Oncol 1992; 31: 99–106
  • Braunstein G D, Karow W G, Gentry W C, Rasor J, Wade M E. First-trimester chorionic gonado tropin measurements as an aid in the diagnosis of early pregnancy disorders. Am J Obstet Gynecol 1978; 131: 25–32
  • Cacciatore B, Ylostalo P, Stenman U-H. Suspected ectopic pregnancy: ultrasound find ings and hCG levels assessed by an immunoflu-orometric assay. Brit J Obstet Gynecol 1988; 95: 497–502
  • Norman R J, Buck R, Reddi K. Urinary hCG test in ectopic pregnancy [letter]. Fertil Steril 1986; 45
  • Cacciatore B, Stenman U-H P. Y. Comparison of abdominal and vaginal sonography in sus pected ectopic pregnancy. Obst Gynecol 1989; 73: 770–774
  • Platt L D, Carlson D E. Prenatal diagnosis -When and how?. N Engl J Med 1992; 327: 636–638
  • Spencer K, Macri J N, Aitken D A, Connor J M. Free beta-hCG as first-trimester marker for fetal trisomy [letter]. Lancet 1992; 339
  • Ozturk M, Milunsky A, Brambati B, Sachs E S, Miller S L, Wands J R. Abnormal maternal serum levels of human chorionic gonadotropin free subunits in trisomy 18. Am J Med Genet 1990; 36: 480–3
  • Jones W B. Gestational trophoblastic disease: what have we learned in the past decade?. Am J Obstet Gynecol 1990; 162: 1286–95
  • Madersbacher S, Klieber R, Mann K, Marth C, Tabarelli M, Wick G, Berger P. Free alpha-sub-unit, free beta-subunit of human chorionic go nadotropin (hCG), and intact hCG in sera of healthy individuals and testicular cancer pa tients. Clin Chem 1992; 38: 370–6
  • Braunstein G D, Vaitukaitis J L, Carbone P P, Ross G T. Ectopic production of human cho rionic gonadotrophin by neoplasms. Ann Int Med 1973; 78: 39–45
  • Stenman U-H, Alfthan H, Ranta T, Vartiainen E, Jalkanen J, Seppala M. Serum levels of human chorionic gonadotropin in nonpregnant women and men are modulated by gonado tropin releasing hormone and sex steroids. J Clin Endocrinol Metab 1987; 64: 730–736
  • Iles R K, Jenkins B J, Oliver R T, Blandy J P, Chard T. Beta human chorionic gonadotrophin in serum and urine. A marker for metastatic uro thelial cancer. Br J Urol 1989; 64: 241–4
  • Alfthan H, Haglund C, Roberts P, Stenman U-H. Elevation of free B subunit of human chorio gonadotropin and core 8 fragment of human choriogonadotropin in serum and urine of pa tients with malignant pancreatic and biliary disease. Cancer Res 1992; 52: 4628–4633
  • Hoermann R, Gerbes A L, Spoettl G, Jüngst D, Mann K. Immunoreactive human chorionic go nadotropin and its free B subunit in serum and ascites of patients with malignant tumors. Cancer Res 1992; 52: 1520–1524
  • Marcillac I, Troalen F, Bidart J-M, Ghillani P, Ribrag V, Escudier B, et al. Free human cho rionic gonadotropin B subunit in gonadal and nongonadal neoplasms. Cancer Res 1992; 52: 3901–3907
  • Weisshaar G, Hiyama J, Renwick A. Site-spe cific N-glycosylation of human chorionic gona dotrophin structural analysis of glycopeptides by one- and two-dimensional 1H NMR spec troscopy. Glycobiology 1991; 1: 393–404
  • Birken S, Gawinowicz M A, Kardana A, Cole L A. The heterogeneity of human chorionic go nadotropin (hCG). II. Characteristics and origins of nicks in hCG reference standards. Endocrinol 1991; 129: 1551–1558
  • Cole L A, Kardana A, Andrade G P, Gawinowicz M A, Morris J C, Bergert E R, et al. The heteroge neity of human chorionic gonadotropin (hCG). III. The occurrence and biological and immuno logical activities of nicked hCG. Endocrinol 1991; 129: 1559–1567
  • Kardana A, Elliott M M, Gawinowicz M-A, Bir ken S, Cole L A. The heterogeneity of human chorionic gonadotropin (hCG). I. Characteriza tion of peptide heterogeneity in 13 individual preparations of hCG. Endocrinol 1991; 129: 1541–1550
  • Lustbader J, Birken S, Pollak S, Levinson L, Bernstine E, Hsiung N, Canfield R. Characteri zation of the expression products of recombi nant human choriogonadotropin and subunits. J Biol Chem 1987; 262: 14204–12
  • Louvet J-P, Ross G T, Birken S, Canfield R E. Absence of neutralizing effect of antisera to the unique structural region of human chorionic gonadotropin. J Clin Endocrinol Metab 1974; 39: 1155–1158
  • Matsuura S, Ohashi M, Chen H C, Hodgen G D. A human chorionic gonadotropin-specific antiserum against synthetic peptide analogs to the carboxyl-terminal peptide of its beta-sub-unit. Endocrinol 1979; 104: 396–401
  • Stevens V C, Chou W S, Powell J E, Lee A C, Smoot J. The identification of peptide se quences of human chorionic gonadotropin con taining a conformational epitope. Immunol Lett 1986; 12: 11–18
  • Troalen F, Razafindratsita A, Puisieux A, Voelt-zel T, Bohuon C, Bellet D, Bidart J M. Structural probing of human lutropin using antibodies raised against synthetic peptides constructed by classical and multiple antigen peptide system approaches. Mol Immunol 1990; 27: 363–8
  • Troalen F, Bellet D H, Ghillani P, Puisieux A, Bohuon C J, Bidart J M. Antigenic determinants on human choriogonadotropin alpha-subunit. II. Immunochemical mapping by a monoclonal antipeptide antibody. J Biol Chem 1988; 263: 10370–10376
  • Talwar G P, Ramakrishnan S, Das C. Recent Advances in Reproduction and Regulation of Fertility, G P Talwar. North Hol land, Amsterdam 1979; 453–466
  • Marshall J R, Hammond C B, Ross G T, Jacobson A, Rayford P, Odell W D. Plasma and urinary chorionic gonadotropin during early human pregnancy. Obstet Gynecol 1968; 32: 760–764
  • Morgan F J, Birken S, Canfield R E. The amino acid sequence of human chorionic gonado tropin: The a subunit and the fi subunit. J Biol Chem 1975; 250: 5247–5258
  • Birken S, Canfield R E. Chemistry and immuno-chemistry of human chorionic gonadotropin. Chorionic gonadotropin, S Segal. Plenum Press, New York 1980; 65–88
  • Birken S, Agosto G, Amr S, Nisula B, Cole L, Lewis J, Canfield R. Characterization of anti-sera distinguishing carbohydrate structures in the pi-carboxyl-terminal region of human cho rionic gonadotropin. Endocrinol 1988; 122: 2054–2063
  • Ehrlich P H, Moustafa Z A, Krichevsky A, Bir ken S, Armstrong E G, Canfield R E. Characteri zation and relative orientation of epitopes for monoclonal antibodies and antisera to human chorionic gonadotropin. Am J Reprod Immu nol Microbiol 1985; 8: 48–54
  • Gupta S K, Talwar G P. Development of hybri-domas secreting anti-human chorionic gonado tropin antibodies. Indian J Exp Biol 1980; 18: 1361–5
  • Bellet D, Bidart J-M, Motte P, Lumbrosco J, Berche C, Schlumberger M, Bohoun C. In vitro and in vivo utilization of monoclonal anti bodies to beta subunit of human chorionic go nadotropin. Hybridoma 1982; 1: 218–9
  • Stenman U-H, Tanner P, Ranta T, Schroder J, Seppala M. Monoclonal antibodies to chorionic gonadotropin: Use in a rapid radioimmunoas say for gynecologic emergencies. Obstet Gyne col 1982; 59: 375–377
  • Stenman U-H, Alfthan H, Myllynen L, Seppala M. Ultrarapid and highly sensitive time-re solved fluoroimmunoassay for human cho rionic gonadotropin. Lancet 1983; 2: 647–649
  • Schwarz S, Berger P, Wick G. The antigenic surface of human chorionic gonadotropin as mapped by murine monoclonal antibodies. En docrinol 1986; 118: 189–197
  • Berger P, Klieber R, Panmoung W, Maders-bacher S, Wolf H, Wick G. Monoclonal anti bodies against free subunits of human cho rionic gonadotropin. J Endocrinol 1990; 125: 301–309
  • Norman R J, Poulton T, Gard T, Chard T. Mono clonal antibodies to human chorionic gonadotropin: Implications for antigenic mapping, im-munoradiometric assays, and clinical applica tions. ] Clin Endocrinol Metab 1985; 61: 1031–1038
  • Bidart J-M, Bellet D H. Structure-Function Relationship of Gona dotropins, D H Bellet, J M Bidart. Raven Press, New York 1989; 21–33
  • Moyle W R, Matzuk M M, Campbell R K, Cogliani E, Dean E D, Krichevsky A, et al. Localiz ation of residues that confer antibody binding specificity using human chorionic gonado tropin/Luteinizing hormone beta subunit chimeras and mutants. J Biol Chem 1990; 265: 8511–8518
  • Ryan R J, Keutmann H T, Charlesworth M C, McCormick D J, Milius R P, Calvo F O, Vutyava-nich H. Structure-function relationships in go nadotropins. Rec Prog Horm Res 1987; 43: 383–429
  • Charlesworth M C, Bergert E R, Morris J C, McCormick D J, Ryan R J. The antigenic struc ture of the human glycoprotein hormone alpha-subunit: III. Solution- and solid-phase mapping using synthetic peptides. Endocrinol 1991; 128: 2907–2915
  • Fiddes J C, Talmadge K. Structure, expression, and evolution of the genes for the human gly coprotein hormones. Rec Prog Horm Res 1984; 40: 43–78
  • Talmadge K, Boorstein W R, Vamvakopoulos N C, Gething M J, Fiddes J C. Only three of the seven human chorionic gonadotropin beta sub-unit genes can be expressed in the placenta. Nucleic Acids Res 1984; 12: 8415–8436
  • Otani T, Otani F, Krych M, Chaplin D D, Boime I. Identification of a promoter region in the CG beta gene cluster [published erratum appears in J Biol Chem 1988 Dec 15;263(35):19256–7]. J Biol Chem 1988; 263: 7322–7329
  • Bo M, Boime I. Identification of the transcrip tionally active genes of the chorionic gonado tropin beta gene cluster in vivo. J Biol Chem 1992; 267: 3179–3184
  • Shen Q X, Bahl O P. cDNA-derived amino acid sequences of choriocarcinoma alpha- and beta-subunits of human choriogonadotropin. Mol Cell Endocrinol 1990; 72: 167–173
  • Pettersson K, Ding Y-D, Huhtaniemi I. An im munological anomalous luteinizing hormone variant in a healthy woman. J Clin Endocrinol Metab 1992; 74: 164–171
  • Amr S, Wehmann R, Birken S, Canfield R, Nisula B. Characterization of a carboxyterminal peptide fragment of the human choriogonado tropin fl-subunit excreted in the urine of a woman with choriocarcinoma. J Clin Invest 1983; 71: 329–339
  • Sakakibara R, Miyazaki S, Ishiguro M. A nicked p-subunit of human chorionic gonadotropin purified from pregnancy urine. J Biochem 1990; 107: 858–862
  • Bidart J-M, Puisieux A, Troalen F, Foglietti M J, Bouhon C, Bellet D. Characterization of a cleav age product in the human choriogonadotropin fi-subunit. Biochem Biophys Res Commun 1988; 154
  • Kardana A, Cole L A. Polypeptide nicks cause erroneous results in assays of human chorionic gonadotropin free S-subunit. Clin Chem 1992; 38: 26–33
  • Birken S, Canfield R E, Lauer R, Agosto G, Gabel M. Immunochemical determinants unique to human chorionic gonadotropin: importance of sialic acid for antisera generated to the human chorionic gonadotropin fi-subunit COOH-ter-minal peptide. Endocrinol 1980; 106: 1659–1664
  • Sairam M R, Linggen J, Bhargavi G N. Alter ations in antigenic structure of gonadotropins following deglycosylation. Biosci Rep 1988; 8: 271–278
  • Wide L, Hobson B. Some qualitative differences of hCG in serum from early and late pregnan cies and trophoblastic diseases. Acta Endocri nol (Copenh) 1987; 116: 465–72
  • Skarulis M C, Wehmann R E, Nisula B C, Blithe D L. Glycosylation changes in human chorionic gonadotropin and free alpha subunits as gesta tion progresses. J Clin Endocrinol Metab 1992; 75: 91–96
  • Stenman U-H, Alfthan H, Turpeinen U. Method dependence of interpretation of immu noassay results. Scand J Clin Lab Invest 1991; 51((Supplement 206))86–94
  • Tyrey L, Hammond C B. The hCG-P-subunit radioimmunoassay: potential error in hCG measurement related to choice of labeled antigen. Am J Obstet Gynecol 1976; 125: 160–165
  • Bellet D H, Ozturk M, Bidart J-M, Bohuon C J, Wands J R. Sensitive and specific assay of human chorionic gonadotropin (hCG) based on anti-peptide and anti-hCG monoclonal anti bodies: construction and clinical implications. J Clin Endocrinol Metab 1986; 63: 1319–1327
  • StockellHartree A, Shownkeen R C. Studies of human pituitary LH containing internally cleaved fi subunit. J Mol Endocrinol 1991; 6: 101–109
  • Papapetrou P D, Sakarelou N P, Braouzi H, Fessas P H. Ectopic production of human cho rionic gonadotropin (hCG) by neoplasms: The value of measurements of immunoreactive hCG in the urine as a screening procedure. Cancer 1980; 45: 2583–2592
  • Wang Y, Schwartz P E, Chambers J T, Cole L A. Urinary gonadotropin fragments (UGF) in cancers of the female reproductive system. II. Initial serial studies. Gynecol Oncol 1988; 31: 91–100
  • O'Connor J F, Schlatterer J P, Birken S, Krichev-sky A, Armstrong E G, McMahon D, Canfield R E. Development of highly sensitive immu noassays to measure human chorionic gonado tropin, its fi-subunit, and fJ core fragment in the urine: Application to malignancies. Cancer Res 1988; 48: 1361–1366
  • Cole L A, Schwartz P E, Wang Y. Urinary gona dotropin fragments (UGF) in cancers of the fe male reproductive system. I. Sensitivity and specificity, comparison with other markers. Gynecol Oncol 1988; 31: 82–90
  • Kardana A, Taylor M E, Southall P J, Boxer G M, Rowan A J, Bagshawe K D. Urinary gonadotro-phin peptide isolation and purification, and its immunohistological distribution in normal and neoplastic tissues. Br J Cancer 1988; 58: 281–286
  • Birken S, Armstrong E G, Kolks M AG, Cole L A, Agosto G M, Krichevsky A, et al. Structure of the human chorionic gonadotropin fi-subunit fragment from pregnancy urine. Endocrinol 1988; 123: 572–583
  • Krichevsky A, Armstrong E G, Schlatterer J, Bir ken S, O'Connor J, Bikel K, et al. Preparation and characterization of antibodies to the uri nary fragment of the human chorionic gonado tropin fi-subunit. Endocrinol 1988; 123: 584–593
  • Krichevsky A, Birken S, O'Connor J, Bikel K, Schlatterer J, Yi C, et al. Development and char acterization of a new, highly specific antibody to the human chorionic gonadotropin-B frag ment. Endocrinol 1991; 128: 1255–1264
  • Weber T H, Kapyaho K I, Tanner P. Endogenous interference in immunoassays in clinical chem istry. A review. Scand J Clin Lab Invest 1990; 50(Suppl 201)77–82
  • Bonner O P. Interference of complement with the binding of carcinoembryonic antigen to solid-phase monoclonal antibodies. J Immunol Meth 1989; 121: 85–93
  • Boerman O C, Segers M F, Poels L G, Kenemans P, Thomas C M. Heterophilic antibodies in human sera causing falsely increased results in the CA 125 immunofluorometric assay [see comments]. Clin Chem 1990; 36: 888–891
  • Turpeinen U, Lehtovirta P, Alfthan H, Stenman U-H. Evaluation of chromatographic methods for removal of immunoglobulin interference in an immunoradiometric assay for CA 125 antigen. Clin Chem 1990; 36: 1333–1338
  • Braunstein G D, Bloch S K, Rasor J L, Winikoff J. Characterization of antihuman chorionic gonadotropin serum antibody appearing after ovu lation induction. J Clin Endocrinol Metab 1983; 57: 1164–72
  • Odell W D, Griffin J. Pulsatile secretion of human chorionic gonadotropin in normal adults. N Engl J Med 1987; 317: 1688–1691
  • Braunstein G D. Pulsatile secretion of human chorionic gonadotropin [letter]. N Engl J Med 1988; 319: 53–4
  • Stenman U-H, Alfthan H, Koskimies A, Seppala M, Pettersson K, Lövgren T. Monitoring the LH surge by ultrarapid and highly sensitive immunofluorometric assay. Ann NY Acad Sci 1985; 442: 544–550
  • Rao C V, Hussa R O, Carmen F R, Rinke M L, Cook C L, Yussman M A. Stability of human chorionic gonadotropin and its alpha subunit in blood. Am J Obstet Gynecol 1983; 146: 65–68
  • deMedeiros S F, Amato F, Norman R J. Stability of immunoreactive |J-core fragment of hCG. Obstet Gynecol 1991; 77: 53–59
  • Wehmann R E, Blithe D L, Akar A H, Nisula B C. Disparity between fi-core levels in pregnancy urine and serum: Implications for the origin of urinary fi-core. J Clin Endocrinol Metab 1990; 70: 371–378
  • Dufau M L, Pock R, Neubauer A, Catt K J. In vitro bioassay of LH in human serum: the rat interstitial cell testosterone (RICT) assay. J Clin Endocrinol Metab 1976; 42: 958–69
  • VanDamme M P, Robertson D M, Diczfalusy E. An improved in vitro bioassay method for measuring luteinizing hormone (LH) activity using mouse Leydig cell preparations. Acta En docrinol (Copenh) 1974; 77: 655–671
  • Kraiem Z, Sadeh O, Yosef M. Iodide uptake and organification, tri-iodothyronine secretion, cy clic AMP accumulation and cell proliferation in an optimized system of human thyroid follicles cultured in collagen suspended in serum-free medium. J Endocr 1991; 131: 499–506
  • Jia X C, Oikawa M, Bo M, Tanaka T, Ny T, Boime I, Hsueh A J. Expression of human luteinizing hormone (LH) receptor: interaction with LH and chorionic gonadotropin from human but not equine, rat, and ovine species. Mol Endocri nol 1991; 5: 759–68
  • Nagayama Y, Kaufman K D, Seto P, Rapoport B. Molecular cloning, sequence and functional expression of the cDNA for the human thyro tropin receptor. Biochem Biophys Res Com-mun 1989; 165: 1184–1190
  • Canfield R E, Ross G T. A new reference prep aration of human chorionic gonadotropin and its subunits. Bull WHO 1976; 54: 436–470
  • WHO. expert committee on biological stand ardization. Twenty-sixth report. Bull Wld Hlth Org Tech Rep Ser 1975, No. 565
  • Bangham D R, Grab B. The Second International Standard for chorionic gonadotrophin. Bull Wld Hlth Org 1964; 31: 111–125
  • Stoning P L, Gaines-Das R E, Bangham D R. In ternational reference preparation of human chorionic gonadotrophin for immunoassay: Potency estimates in various bioassay and pro tein binding assay systems; and international reference preparations of a and fi subunits of human chorionic gonadotrophin for immu noassay. J Endocrinol 1980; 84: 295–310
  • WHO. expert committee on biological stand ardization. Thirty-seventh report. Bull Wld Hlth Org Tech Rep Ser 1987, No. 760
  • Moss J, Ross P, Agosto G, Birken S, Canfield R, Vaughan M. Mechanism of action of choleragen and glycopeptide hormones. Is the nicotina mide adenine dinucleotide glycohydrolase ac tivity observed in purified hormon prepara tions intrinsic to the hormone? Endocrinology 1978; 102: 415–419
  • Wehmann R E, Blithe D L, Akar A H, Nisula B C. 6-core fragments are contaminants of the World Health Organization reference prepara tions of human choriogonadotropin and its a-subunit. J Endocrinol 1988; 117: 147–152
  • Mochizuki M, Nishimura R, Kobata A., Belief D, Bidart J M. Structure-function of gonado tropins. Raven Press, New York 1989; 45–58
  • Pollak S, Halpine S, Chait B T, Birken S. High resolution high performance liquid chromato graphy fingerprinting of purified human cho rionic gonadotropin demonstrates that oxida tion is a cause of hormone heterogeneity. Endo crinol 1990; 126: 199–208
  • Chard T. An introduction to radioimmunoas say and related techniques. Else vier, New York 1987
  • Ekins R P. The precision profile: Its use in assay design, assessment and quality control. Immunoassays for clinical chemistry, W M Hunter, JET Corrie. Churchill Living stone, New York 1983; 76–105
  • Ragget P. Data processing. Principles and practice of immu noassay, C P Price, D J New man. Stockton press, New York 1991; 190–218
  • Sturgeon C, Seth J, Al-Sadie R. Comm Lab Med 1992; 1: 39–40
  • Wilcox A J, Weinberg C R, O'Connor J F, Baird D D, Schlatterer J P, Canfield R E, et al. Incidence of early loss of pregnancy. N Engl J Med 1988; 319: 189–94
  • Stenman U-H. Sensitive pregnancy tests. Duodecim 1988; 104: 677–9
  • Wald N J, Cuckle H S, Densem J W, Nanchahal K, Royston P, Chard T, et al. Maternal serum screening for Down's syndrome in early preg nancy [published erratum appears in BMJ 1988 Oct 22;297(6655):1029]. Brit Med J 1988; 297: 883–7
  • Spencer K. Evaluation of an assay of the free beta-subunit of choriogonadotropin and its potential value in screening for Down's syn drome [see comments]. Clin Chem 1991; 37: 809–14
  • Thomas C M, Segers M F, Leloux A M, Houx P C. Comparison of the analytical characteristics of ten urinary hCG tests for early pregnancy diag nosis. Ann Clin Biochem 1986
  • Bartlett N L, Freiha F S, Torti F M. Serum markers in germ cell neoplasms. Hematol Oncol Clin North Am 1991; 5: 1245–1260
  • Bates S E. Clinical applications of serum tumor markers. Ann fnt Med 1991; 115: 623–638
  • Huff B C. Gestational trophoblastic disease. NAACOGS Clin Issu Perinat Womens Health Nurs 1990; 1: 453–458
  • Berkowitz R S, Goldstein D P. Diagnosis and management of the primary hydatidiform mole. Obstet Gynecol Clin North Am 1988; 15
  • Miller D S, Lurain J R. Classification and staging of gestational trophoblastic tumors. Obstet Gynecol Clin North Am 1988; 15: 477–490
  • Khazaeli M B, Hedayat M M, Hatch K D, To A CW, Soong S-J, Shingleton H M, et al. Radioimmunoassay of free 6-subunit of human chorionic gonadotropin as a prognostic test for persisitent trophoblastic disease in molar preg nancy. Am J Obstet Gynecol 1986; 155: 320–324
  • Ozturk M, Berkowitz R, Goldstein D, Belief D, Wands J R. Differential production of human chorionic gonadotropin and free subunits in gestational trophoblastic disease. Am J Obstet Gynecol 1988; 158: 193–8
  • Hussa R O. Human chorionic gonadotropin, a clinical marker: review of its biosynthesis. Li-gand Review 1981; 3: 6–44
  • Murphy P, Johnson D H. Staging and prognos tic factors in nonseminomatous testicular cancer. Hematol Oncol Clin North Am 1991; 5: 1233–1243
  • Aass N, Klepp O, Cavallin-Stahl E, Dahl O, Wicklund H, Unsgaard B, et al. Prognostic fac tors in unselected patients with nonseminomatous metastatic testicular cancer: a multicenter experience. J Clin Oncol 1991; 9: 818–826
  • Jensen J L, Venner P M. Predictive factors for outcome in treatment of metastatic nonsemino matous germ cell tumors. Urology 1992; 39: 237–242
  • Horwich A, Peckham M J. Transient rumor mar ker elevation following chemotherapy for germ cell tumors of the testis. Cancer Treat Rep 1986; 70: 1329–1331
  • Lange P H, Nochomovitz L E, Rosai J, Fraley E E, Kennedy B J, Bosl G, et al. Serum alpha-fetopro-tein and human chorionic gonadotropin in pa tients with seminoma. J Urol 1980; 124: 472–8
  • Sailer B, Clara R, Spottl G, Siddle K, Mann K. Testicular cancer secretes intact human chorio gonadotropin (hCG) and its free beta-subunit: evidence that hCG (+hCG-beta) assays are the most reliable in diagnosis and follow-up [see comments]. Clin Chem 1990; 36: 234–9
  • Mumperow E, Hartmann M. Spermatic cord beta-human chorionic gonadotropin levels in seminoma and their clinical implications. J Urol 1992; 147: 1041–3
  • Iles R K, Purkis P E, Whitehead P C, Oliver R T, Leigh I, Chard T. Expression of beta human chorionic gonadotrophin by non-trophoblastic non-endocrine ‘normal’ and malignant epithe lial cells. Br J Cancer 1990; 61: 663–6
  • Oberg K, Wide L. hCG and hCG subunits as tumour markers in patients with endcocrine pancreatic tumours and carcinoids. Acta Endo crinol 1981; 98: 256–260
  • Cole L A, Birken S. Origin and occurrence of human chorionic gonadotropin p-subunit core fragment. Mol Endocrinol 1988; 2: 826–830
  • Ruddon R W, Anderson C, Meade K S, Alden-derfer P H, Neuwald P D. Content of gonado tropins in cultured human malignant cells and effects of sodium butyrate treatment on gona dotropin secretion by HeLa cells. Cancer Res 1979; 39: 3885–92
  • Iles R K, Oliver R TD, Kitau M, Walker C, Chard T. In vitro secretion of human chorionic gona dotrophin by bladder tumour cells. Br J Cancer 1987; 55: 623–626
  • Hoermann R, Spoettl G, Moncayo R, Mann K. Evidence for the presence of human chorionic gonadotropin (hCG) and free beta-subunit of hCG in the human pituitary. J Clin Endocrinol Metab 1990; 71: 179–86

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