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Scandinavian Journal of Gastroenterology Volume 38, 2003 - Issue 7
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Research Article

Applicability of the Ussing Chamber Technique to Permeability Determinations in Functionally Distinct Regions of the Gastrointestinal Tract in the Rat

Bajka B. H. Child Health Research Institute and Cooperative Research Centre for Tissue Growth and Repair Adelaide South Australia
,
Gillespie C. M. Depts. of Physiology and Paediatrics University of Adelaide Adelaide South Australia
,
Steeb C.-B. Gastroenterology Dept. Women's and Children's Hospital Adelaide South Australia
,
Read L. C. Child Health Research Institute and Cooperative Research Centre for Tissue Growth and Repair Adelaide South Australia
&
Howarth G. S. Depts. of Physiology and Paediatrics University of Adelaide Adelaide South Australia
Pages 732-741 | Published online: 08 Jul 2009

  • Hollander D. Intestinal permeability, leaky gut, and intestinal disorders. Curr Gastroenterol Rep 1999:1:410-6.
  • Bijlsma FB, Peelers RA, Groot JA, Dekker PR, Taminiau JA, Van Der Meer R. Differential in vivo and in vitro intestinal permeability to lactulose and mannitol in animals and humans: a hypothesis. Gastroenterology 1995:108:687-96.
  • Urban E, Smith NL, Smith TC. Calcium transport by the normal rat colon in vivo and in vitro. Digestion 1978; 17:69-83.
  • Hyun CS, Chen CWP, Shinowara NL, Palaia T, Fallick FS, Martello LA, et al. Morphological factors influencing transepithelial conductance in a rabbit model of ileitis. Gastroenterology 1995:109:13-23.
  • Riegler M, Feil W, Lindroth J, Wong M. MIPSY: Real-time morphology to quantify the time course of rapid epithelial restitution. Path Res Pract 1992:188:443-8.
  • Suzuki H, Yanaka A, Muto H. Luminal ammonia retards restitution of guinea pig injured gastric mucosa in vitro. Am J Physiol 2000;279:G107-17.
  • Matysiak-Budnik T, de Mascarel A, Abely M, Mayo K, Heyman M, Megraud F. Positive effect of rebamipide on gastric permeability in mice after eradication of Helicobacter felis. Scand J Gastroenterol 2000;35:preceding 1233.
  • Venkatraman A, Ramakrishna BS, Pulimood AB. Butyrate hastens restoration of barrier function after thermal and detergent injury to rat distal colon in vitro. Scand J Gastroenterol 1999:34:1087-92.
  • Smecuol E, Bai JC, Sugai E, Vazquez H, Niveloni S, Pedreira S, et al. Acute gastrointestinal permeability responses to different non-steroidal anti-inflammatory drugs. Gut 2001 ;49:650-5.
  • Curds GH, MacNaughton WK, Gall DG. Effect of cyclooxygenase inhibition on macromolecular transport in rat gastric mucosa. Am J Physiol 1993065 (6 Pt 1):G1135-40.
  • Faggio C, Denaro MG, Lionetto MG, Trischitta F. Protective effects of prostaglandins in the isolated gastric mucosa of the eel, Anguilla anguilla. J Comp Physiol [B] 2000;170:357-63.
  • Drago F, Montoneri C, Varga C, Laszlo F. Dual effect of female sex steroids on drug-induced gastroduodenal ulcers in the rat. Life Sci 1999:64:2341-50.
  • Noms HT. Response of the small intestine to the application of a hypertonic solution. Am J Pathol 1973;73:747-64.
  • Riegler M, Sedivy R, Sogukoglu T, Bischof G, Teleky B, Feil W, et al. Effect of growth factors on epithelial restitution of human colonie mucosa in vitro. Scand J Gastroenterol 1998;234: 925-32.
  • Marchbank T, Cox HM, Goodlad RA, Giraud AS, Moss SF, Poulsom R, et al. Effect of ectopic expression of rat trefoil factor family 3 (intestinal trefoil factor) in jejunum of transgenic mice. J Biol Chem 2001:276:240888-96.
  • Puspok A, Oberhuber G, Wyatt J, Maier-Dobersberger T, Hammer J, Pfeffel F, et al. Gastroduodenal permeability in Crohn's disease. Eur J Clin Invest 199828:67-71.
  • Mariadason JM, Kilias D, Catto-Smith A, Gibsin PR. Effect of butyrate on paracellular permeability in rat distal colonie mucosa ex vitro. J Gastroenterol Hepatol 1999:14:873-9.
  • Fromm M, Schulzke J-D, Hegel U. Epithelial and sudepithelial contributions to tramsmural electrical resistance of intact rat jejunum, in vitro. Pflügers Arch 1985:405:400-2.
  • Langeler EG, van Hinsbergh VW. Characterization of an in vitro model to study the permeability of human arterial endothelial cell monolayers. Thromb Haemost 1988;60:240-6.
  • Worthington BS, Meserole L, Syrotuck JA. Effect of daily ethanol ingestion on intestinal permeability to macromolecules. Am J Dig Dis 197823:23-32.
  • Machen TE, McLennan WL. Na+-dependent H+ and Cl transport in in vitro frog gastric mucosa. Am J Physiol 1980; 238:G403-13.
  • Bakos P, Jezova D. Ion transport in rat antrat mucosa in vitro: general characteristics. Gen Physiol Biophys 1995;14:473-90.
  • Yanaka A, Muto H, Fukutomi H, Ito S, Silen W. Role of transforming growth factor-beta in the restitution of injured guinea pig gastric mucosa in vitro. Am J Physiol 1996;271 (1 Pt 1):G75-85.
  • Venkatraman A, Ramakrishna BS, Pulimood AB, Patra S, Murthy S. Increased permeability in dextran sulphate colitis in rats: time course of development and effect of butyrate. Scand J Gastroenterol 2000;35:1053-9.
  • Yates DA, Santos J, Soderholm JD, Perdue MH. Adaptation of stress-induced mucosal pathophysiology in rat colon involves opioid pathways. Am J Physiol 2001;281:G124-8.
  • Winckler C, Breves G, Boll M, Daniel H. Characteristics of dipeptide transport in pig jejunum in vitro. J Comp Physiol [B] 1999:169:445-50.
  • Schulzke JD, Schulzke I, Fromm M, Riecken EO. Epithelial barrier and ion transport in coeliac sprue: electrical measurements on intestinal aspiration biopsy specimens. Gut 1995;37: 777-82.
  • Yang PC, Berin MC, Yu L, Perdue MH. Mucosal pathophysiology and inflammatory changes in the late phase of the intestinal allergic reaction in the rat. Am J Pathol 2001;158:681-90.
  • Yang H, Soderholm JD, Larsson J, Permert J, Lindgren J, Wiren M. Bidirectional supply of glutamine maintains enterocyte ATP content in the in vitro Ussing chamber model. Int J Colorectal Dis 2000:15:291-6.
  • Soderholm JD, Hedman L, Artursson P, Franzen L, Larsson J, Pantzar N, et al. Integrity and metabolism of human ileal mucosa in vitro in the Ussing chamber. Acta Physiol Scand 1998:162:47-56.
  • Armstrong WM. Cellular mechanisms of ion transport in the small intestine. In: Johnson LR, editor. Physiology of the gastrointestinal tract, 2nd ed. New York: Raven Press; 1987. p. 1251-65.
  • Catto-Smith AG, Hardin JA, Patrick MK, O'Loughlin EV, Gall DG. The effect of atrial natriuretic peptide on intestinal electrolyte transport. Regul Pept 1991:36:29-44.
  • Kuge T, Venkova K, Greenwood-Van Meerveld B. In vitro effects of wood creosote on enterotoxin-induced secretion measured electrophysiologjcally in the rat jejunum and colon. Biol Pharm Bull 2001:24:623-7.

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