Expanding hepatocytes in vitro before cell transplantation: donor age‐dependent proliferative capacity of cultured human hepatocytes

References

• Wang X, Andersson R. Hepatocyte transplantation: a potential treatment for acute liver failure. Scand J Gastroenterol 1995;30: 193–200.
   PubMed Web of Science ®Google Scholar
• Fox IJ, Chowdhury JR, Kaufman SS, Goertzen TC, Chowdhury NR, Warkentin PI, et al. Treatment of the Crigler-Najjar syndrome type I with hepatocyte transplantation. N Engl J Med 1998;338:1422–6.
   PubMed Web of Science ®Google Scholar
• Malhi H, Gupta S. Hepatocyte transplantation: new horizons and challenges. J Hepatobiliary Pancreat Surg 2001;8:40–50.
   PubMedGoogle Scholar
• Guha C, Deb NJ, Sappal BS, Ghosh SS, Roy-Chowdhury N, Roy-Chowdhury J. Amplification of engrafted hepatocytes by preparative manipulation of the host liver. Artif Organs 2001;25: 522–8.
   Google Scholar
• Tsiaoussis J, Newsome PN, Nelson LJ, Hayes PC, Plevris JN. Which hepatocyte will it be? Hepatocyte choice for bioartificial liver support systems. Liver Transpl 2001;7:2–10.
   Google Scholar
• Fausto N. Liver regeneration. J Hepatol 2000;32 Suppl 1:19–31.
   PubMed Web of Science ®Google Scholar
• Runge DM, Runge D, Dorko K, Pisarov LA, Leckel K, Kostrubsky VE, et al. Epidermal growth factor- and hepatocyte growth factor-receptor activity in serum-free cultures of human hepatocytes. J Hepatol 1999;30:265–74.
   PubMed Web of Science ®Google Scholar
• Iakova P, Awad SS, Timchenko NA. Aging reduces proliferative capacities of liver by switching pathways of C/EBPalpha growth arrest. Cell. 2003;113:495–506.
   Google Scholar
• Palmer HJ, Tuzon CT, Paulson KE. Age-dependent decline in mitogenic stimulation of hepatocytes. Reduced association between Shc and the epidermal growth factor receptor is coupled to decreased activation of Raf and extracellular signal-regulated kinases. J Biol Chem 1999;274:11424–30.
   Google Scholar
• Ismail T, Howl J, Wheatley M, McMaster P, Neuberger JM, Strain AJ. Growth of normal human hepatocytes in primary culture: effect of hormones and growth factors on DNA synthesis. Hepatology 1991;14:1076–82.
   PubMed Web of Science ®Google Scholar
• Diehl AM. Liver regeneration. Front Biosci 2002;7:e301–14.
   PubMed Web of Science ®Google Scholar
• Loyer P, Cariou S, Glaise D, Bilodeau M, Baffet G, Guguen-Guillouzo C. Growth factor dependence of progression through G1 and S phases of adult rat hepatocytes in vitro. Evidence of a mitogen restriction point in mid-late G1. J Biol Chem 1996;271: 11484–92.
   Google Scholar
• Heichman KA, Roberts JM. Rules to replicate by. Cell 1994;79: 557–62.
   PubMed Web of Science ®Google Scholar
• Menjo M, Ikeda K, Nakanishi M. Regulation of G1 cyclin-dependent kinases in liver regeneration. J Gastroenterol Hepatol 1998;13 Suppl:100–5.
   Google Scholar
• Peter M, Herskowitz I. Joining the complex: cyclin-dependent kinase inhibitory proteins and the cell cycle. Cell 1994;79:181–4.
   PubMed Web of Science ®Google Scholar
• Walldorf J, Koehler C, Cai H, Kleist S, Runge D, Strom S, et al. The expression of cell cycle related proteins in rat hepatocytes is distinct from human hepatocytes when cultured in serum-free media supplemented with HGF (hepatocyte growth factor) and EGF (epidermal growth factor). J Hep 2002;36 Suppl 1:68.
   Web of Science ®Google Scholar
• Strom SC, Dorko K, Thompson MT, Pisarov LA, Nüssler AK. Large scale isolation and culture of human hepatocytes. In: Franco D, Boudjema K, Varet B, editors. Ilots de Langerhans et hepatocytes. Vers une untilisation therapeutique. Paris: 1998. p. 195–205.
   Google Scholar
• Runge D, Kohler C, Kostrubsky VE, Jayer D, Lehmann T, Runge DM, et al. Induction of cytochrome P450 (CYP)1A1, CYP1A2, and CYP3A4 but not of CYP2C9, CYP2C19, multidrug resistance (MDR-1) and multidrug resistance associated protein (MRP-1) by prototypical inducers in human hepatocytes. Biochem Biophys Res Commun 2000;273:333–41.
   PubMed Web of Science ®Google Scholar
• Awad MM, Gruppuso PA. Cell cycle control during liver development in the rat: evidence indicating a role for cyclin D1 posttranscriptional regulation. Cell Differ 2000;11:325–34.
   Google Scholar
• Trotter J. WinMDI Version 2.8 (freeware). 2000 (http:// facs.scripps.edu).
   Google Scholar
• Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227 (259):680–5.
   PubMed Web of Science ®Google Scholar
• Gorla GR, Malhi H, Gupta S. Polyploidy associated with oxidative injury attenuates proliferative potential ofcells. J Cell Sci 2001;114:2943–51.
   Google Scholar
• Tsukamoto I, Nakata R, Kojo S. Effect of ageing on rat liver regeneration after partial hepatectomy. Biochem Mol Biol Int 1993;30:773–8.
   PubMedGoogle Scholar
• Sigal SH, Gupta S, Gebhard DF, Jr, Holst P, Neufeld D, Reid LM. Evidence for a terminal differentiation process in the rat liver. Differentiation 1995;59:35–42.
   Google Scholar
• Michalopoulos GK, Bowen W, Nussler AK, Becich MJ, Howard TA. Comparative Analysis of mitogenic and morphogenic effects of HGF and EGF on rat and human hepatocytes maintained in collagen gels. J Cell Physiol 1993;156:443–52.
   Google Scholar
• Schmucker DL. Hepatocyte fine structure during maturation and senescence. J Electron Microsc Tech 1990;14:106–25.
   PubMedGoogle Scholar
• Katayama S, Tateno C, Asahara T, Yoshizato K. Size-dependent in vivo growth potential of adult rat hepatocytes. Am J Pathol 2001;158:97–105.
   PubMed Web of Science ®Google Scholar
• Hieter P, Griffiths T. Polyploidy—more is more or less. Science 1999;285 (5425):210–1.
   PubMed Web of Science ®Google Scholar
• Rajvanshi P, Liu D, Ott M, Gagandeep S, Schilsky ML, Gupta S. Fractionation of rat hepatocyte subpopulations with varying metabolic potential, proliferative capacity, and retroviral gene transfer efficiency. Exp Cell Res. 1998;244:405–19.
   PubMed Web of Science ®Google Scholar
• Overturf K, Al-Dhalimy M, Finegold M, Grompe M. The repopulation potential of hepatocyte populations differing in size and prior mitotic expansion. Am J Pathol 1999;155:2135–43.
   PubMed Web of Science ®Google Scholar
• Weglarz TC, Degen JL, Sandgren EP. Hepatocyte transplantation into diseased mouse liver. Kinetics of parenchymal repopulation and identification of the proliferative capacity of tetraploid and octaploid hepatocytes. Am J Pathol 2000;157:1963–74.
   Google Scholar
• Mitry RR, Hughes RD, Aw MM, Terry C, Mieli-Vergani G, Girlanda R, et al. Human hepatocyte isolation and relationship of cell viability to early graft function. Cell Transplant 2003;12:69–74.
   Google Scholar
• Waring JF, Ciurlionis R, Jolly RA, et al. Isolated human hepatocytes in culture display markedly different gene expression patterns depending on attachment status. Toxicol In Vitro 2003; 17:693–701.
   PubMed Web of Science ®Google Scholar
• Gerland LM, Ffrench M, Magaud JP. Cyclin dependent kinase inhibitors and replicative senescence. Pathol Biol (Paris) 2001; 49:830–9.
   PubMedGoogle Scholar
• Stein GH, Dulic V. Molecular mechanisms for the senescent cell cycle arrest. J Investig Dermatol Symp Proc 1998;3:14–8.
   PubMedGoogle Scholar