274
Views
47
CrossRef citations to date
0
Altmetric
Original Article

Broad-range PCR as a supplement to culture for detection of bacterial pathogens in patients with a clinically diagnosed spinal infection

, , &
Pages 772-777 | Received 05 Jan 2008, Published online: 08 Jul 2009

References

  • Butler JS, Shelly MJ, Timlin M, Powderly WG, O'Byrne JM. Non-tuberculous pyogenic spinal infection in adults: a 12-y experience from a tertiary referral centre. Spine 2006; 31: 2695–700
  • Chelsom J, Solberg CO. Vertebral osteomyelitis at a Norwegian university hospital 1987–1997: clinical features, laboratory findings and outcome. Scand J Infect Dis 1998; 30: 147–51
  • Ozuna RM, Delamarter RB. Pyogenic vertebral osteomyelitis and post-surgical disc space infections. Orthop Clin North Am 1996; 27: 87–94
  • Fenollar F, Raoult D. Molecular diagnosis of bloodstream infections caused by non-cultivable bacteria. Int J Antimicrob Agents 2007; 30(Suppl 1)S7–15
  • Zucol F, Ammann RA, Berger C, Aebi C, Altwegg M, Niggli FK, et al. Real-time quantitative broad-range PCR assay for detection of the 16S rRNA gene followed by sequencing for species identification. J Clin Microbiol 2006; 44: 2750–9
  • Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA, Stratton C. Manual of clinical microbiology, 8th edn: Manual of clinical microbiology, 8th edn. 46 ed. 2005.
  • Hall KK, Lyman JA. Updated review of blood culture contamination. Clin Microbiol Rev 2006; 19: 788–802
  • Ley BE, Linton CJ, Bennett DM, Jalal H, Foot AB, Millar MR. Detection of bacteraemia in patients with fever and neutropenia using 16S rRNA gene amplification by polymerase chain reaction. Eur J Clin Microbiol Infect Dis 1998; 17: 247–53
  • Fenollar F, Roux V, Stein A, Drancourt M, Raoult D. Analysis of 525 samples to determine the usefulness of PCR amplification and sequencing of the 16S rRNA gene for diagnosis of bone and joint infections. J Clin Microbiol 2006; 44: 1018–28
  • Fihman V, Hannouche D, Bousson V, Bardin T, Liote F, Raskine L, , et al Improved diagnosis specificity in bone and joint infections using molecular techniques. J Infect 2007;55:510–7.
  • Lecouvet F, Irenge L, Vandercam B, Nzeusseu A, Hamels S, Gala JL. The aetiological diagnosis of infectious discitis is improved by amplification-based DNA analysis. Arthritis Rheum 2004; 50: 2985–94
  • Muyzer G, de Waal EC, Uitterlinden AG. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 1993; 59: 695–700
  • Ohene-Adjei S, Chaves AV, McAllister TA, Benchaar C, Teather RM, Forster RJ. Evidence of increased diversity of methanogenic archaea with plant extract supplementation. Microb Ecol 2007. Published Online Dec 13.
  • Sun W, Dong L, Kaneyama K, Takegami T, Segami N. Bacterial diversity in synovial fluids of patients with TMD determined by cloning and sequencing analysis of the 16S ribosomal RNA gene. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;105:566–71.
  • Rovery C, Greub G, Lepidi H, Casalta JP, Habib G, Collart F, et al. PCR detection of bacteria on cardiac valves of patients with treated bacterial endocarditis. J Clin Microbiol 2005; 43: 163–7
  • Houpikian P, Raoult D. Blood culture-negative endocarditis in a reference centre: aetiological diagnosis of 348 cases. Medicine (Baltimore) 2005; 84: 162–73
  • Kupila L, Rantakokko-Jalava K, Jalava J, Nikkari S, Peltonen R, Meurman O, et al. Aetiological diagnosis of brain abscesses and spinal infections: application of broad-range bacterial polymerase chain reaction analysis. J Neurol Neurosurg Psychiatry 2003; 74: 728–33
  • Voldstedlund M, Norum PL, Baandrup U, Klaaborg KE, Fuursted K. Broad-range PCR and sequencing in routine diagnosis of infective endocarditis. APMIS 2008; 116: 190–8

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.