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- HPLC was carried out using the following equipment and condition: Altex pump model 110A. Hitachi variable-wavelength UV-VIS spectrophotometer detector model 155–40. Altex sample injection valve 210. Kipp and Zonen recorder model BD-41. Prepacked Beckman Ultrasphere ODS (particle size 5 μm) stainless-steel column (25 cm × 4.6 mm I.D.) equipped with ODS pre-column (4.5 cm × 4.6 mm I.D.). Mobile phase consisted of methanol-water (75:25 v/v). Flow rate 1 ml/min. Detection at 254 nm
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