Optimizing immunofluorescent staining of H vessels within an irradiated fracture callus in paraffin-embedded tissue samples

References

• Peng Y, Wu S, Li Y, et al. Type H blood vessels in bone modeling and remodeling. Theranostics. 2020;10(1):426–436. doi: 10.7150/thno.34126
   PubMed Web of Science ®Google Scholar
• Ramasamy SK, Kusumbe AP, Itkin T, et al. Regulation of hematopoiesis and osteogenesis by blood vessel-derived signals. Annu Rev Cell Dev Biol. 2016 Oct 6;32:649–675. doi: 10.1146/annurev-cellbio-111315-124936
   PubMed Web of Science ®Google Scholar
• Zhang J, Pan J, Jing W. Motivating role of type H vessels in bone regeneration. Cell Prolif. 2020 Sep;53(9):e12874. doi: 10.1111/cpr.12874
   PubMed Web of Science ®Google Scholar
• Kusumbe AP, Ramasamy SK, Adams RH. Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone. Nature. 2014 Mar 20;507(7492):323–328. doi: 10.1038/nature13145
   PubMed Web of Science ®Google Scholar
• Wang L, Zhou F, Zhang P, et al. Human type H vessels are a sensitive biomarker of bone mass. Cell Death Dis. 2017 May 4;8(5):e2760. doi: 10.1038/cddis.2017.36
   PubMedGoogle Scholar
• Wang Q, Zhou J, Wang X, et al. Coupling induction of osteogenesis and type H vessels by pulsed electromagnetic fields in ovariectomy-induced osteoporosis in mice. Bone. 2022 Jan;154:116211. doi: 10.1016/j.bone.2021.116211
   PubMed Web of Science ®Google Scholar
• Li Y, Zhu J, Zhang X, et al. Drug-delivery nanoplatform with synergistic regulation of angiogenesis-osteogenesis coupling for promoting vascularized bone regeneration. ACS Appl Mater Interfaces. 2023 Apr 12;15(14):17543–17561. doi: 10.1021/acsami.2c23107
   PubMed Web of Science ®Google Scholar
• Donaubauer AJ, Deloch L, Becker I, et al. The influence of radiation on bone and bone cells—differential effects on osteoclasts and osteoblasts. Int J Mol Sci. 2020 Sep 2;21(17):6377. doi: 10.3390/ijms21176377
   PubMed Web of Science ®Google Scholar
• Singh A, Veeriah V, Xi P, et al. Angiocrine signals regulate quiescence and therapy resistance in bone metastasis. JCI Insight. 2019 Jul 11;4(13). doi: 10.1172/jci.insight.125679
   Web of Science ®Google Scholar
• Soares CBG, Araujo ID, Padua BJ, et al. Pathological fracture after radiotherapy: systematic review of literature. Rev Assoc Med Bras (1992). 2019 Jul 22;65(6):902–908. doi: 10.1590/1806-9282.65.6.902
   PubMed Web of Science ®Google Scholar
• Snyder AG, Oberst A. The antisocial network: cross talk between cell death programs in host defense. Annu Rev Immunol. 2021 Apr 26;39(1):77–101. doi: 10.1146/annurev-immunol-112019-072301
   PubMedGoogle Scholar
• Kesavardhana S, Malireddi RKS, Kanneganti TD. Caspases in cell death, inflammation, and pyroptosis. Annu Rev Immunol. 2020 Apr 26;38(1):567–595. doi: 10.1146/annurev-immunol-073119-095439
   PubMedGoogle Scholar
• Kusumbe AP, Ramasamy SK, Starsichova A, et al. Sample preparation for high-resolution 3D confocal imaging of mouse skeletal tissue. Nat Protoc. 2015 Dec;10(12):1904–1914. doi: 10.1038/nprot.2015.125
   PubMed Web of Science ®Google Scholar
• Robertson D, Savage K, Reis-Filho JS, et al. Multiple immunofluorescence labelling of formalin-fixed paraffin-embedded (FFPE) tissue. BMC Cell Biol. 2008 Mar 19;9(1):13. doi: 10.1186/1471-2121-9-13
   PubMed Web of Science ®Google Scholar
• Tips and techniques for troubleshooting immunohistochemistry. [cited 2023 Sep 13]. Available from: https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/immunohistochemistry/antibody-immunohistochemistry-expert-tips-and-techniques
   Google Scholar
• Panvini FM, Pacini S, Mazzoni S, et al. Multicolor immunofluorescence staining of paraffin-embedded human bone marrow sections. Methods Mol Biol. 2021;2308:119–126. doi: 10.1007/978-1-0716-1425-9_10
   PubMedGoogle Scholar
• Zaqout S, Becker LL, Kaindl AM. Immunofluorescence staining of paraffin sections step by step. Front Neuroanat. 2020;14:582218. doi: 10.3389/fnana.2020.582218
   PubMed Web of Science ®Google Scholar
• Donneys A, Weiss DM, Deshpande SS, et al. Localized deferoxamine injection augments vascularit`y and improves bony union in pathologic fracture healing after radiotherapy. Bone. 2013 Jan;52(1):318–325. doi: 10.1016/j.bone.2012.10.014
   PubMed Web of Science ®Google Scholar
• Zhao Y, Xie L. Unique bone marrow blood vessels couple angiogenesis and osteogenesis in bone homeostasis and diseases. Ann N Y Acad Sci. 2020 Aug;1474(1):5–14. doi: 10.1111/nyas.14348
   PubMed Web of Science ®Google Scholar
• Holden P, Nair LS. Deferoxamine: an angiogenic and antioxidant molecule for tissue regeneration. Tissue Eng Part B Rev. 2019 Dec;25(6):461–470. doi: 10.1089/ten.TEB.2019.0111
   PubMed Web of Science ®Google Scholar