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Original Articles

Synergistic Use of Countercurrent Chromatography and High Performance Liquid Chromatography for the Purification of Synthetic Peptides

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Pages 341-350 | Published online: 13 Dec 2006

References

  • Abbreviations: CCC = countercurrent chromatography; HPLC = high performance liquid chromatography; TFA = trifluoroacetic acid; LAP = leucine aminopeptidase; CPA = carboxypeptidase A; TLC = thin-layer chromatography
  • Putterman , G. J. , Perini , F. , White , E. L. and Ito , Y. Purification of Synthetic Peptides By Means of Countercurrent Chromatography With the Horizontal Flow-Through Coil Planet Centrifuge. Peptides: Structure and Biological Function . Proc. Sixth American Peptide Symposium . Edited by: Gross , E. and Meienhofer , J. pp. 113 Rockford, IL : Pierce Chemical Co. .
  • Ito , Y. and Putterman , G. J. 1980 . New Horizontal Flow-Through Coil Planet Centrifuge For Countercurrent Chromatography III. Separation and Purification of Dinitrophenyl Amino Acids and Peptides . J. Chromatogr. , 193 : 37
  • Aldenderfer , P. H. , Meade-Cobun , K. S. and Putterman , G. J. Unpublished results
  • Merrifield , R. B. 1963 . Solid Phase Peptide Synthesis I. The Synthesis of a Tetrapeptide . J. Amer. Chem. Soc. , 85 : 2149
  • Gisin , B. F. 1973 . The Preparation of Merrifield-Resins Through Total Esterification With Cesium Salts . Helvet. Chim. Acta , 56 : 1476
  • Ito , Y. 1980 . New Horizontal Flow-Through Coil Planet Centrifuge For Countercurrent Chromatography II. The Apparatus and Its Partition Capabilities . J. Chromatogr. , 188 : 43
  • Putterman , G. J. , Spear , M. B. , Meade-Cobun , K. S. , Widra , M. and Hixson , C. V. 1982 . A Rapid Isolation of Human Chorionic Gonadotropin and Its Subunits By Reversed-Phase High Performance Liquid Chromatography . J. Liq. Chromatogr. , 5 : 715
  • Wellner , D. and Lichtenberg , L. A. 1971 . Assay of Amino Acid Oxidase . Methods in Enzymol. , 17B : 593
  • Knight , M. , Ito , Y. and Chase , T. N. 1981 . Preparative Purification of the Peptide Des-Enkephalin-γ-Endorphin. Comparison of High-Performance Liquid Chromatography and Countercurrent Chromatography . J. Chromatogr. , 212 : 356
  • Knight , M. , Tamminga , C. A. and Chase , T. N. Purification of Synthetic Peptides by Countercurrent Chromatography on the Coil Planet Centrifuge . Abstr. Pittsburgh Conf. Anal. Chem. and Applied Spectros . pp. 614 Atlantic City

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