‘Follow the Water’: Hydrogeochemical Constraints on Microbial Investigations 2.4 km Below Surface at the Kidd Creek Deep Fluid and Deep Life Observatory

References

• Andrews JN, Davis SN, Fabryka-Martin J, Fontes J-C, Lehmann BE, Loosli HH, Michelot J-L, Moser H, Smith B, Wolf M. 1989. The in situ production of radioisotopes in rock matrices with particular reference to the Stripa granite. Geochim Cosmochim Acta 53(8):1803–1815.
   Web of Science ®Google Scholar
• Bach W, Edwards KJ. 2003. Iron and sulfide oxidation within the basaltic ocean crust: implications for chemolithoautotrophic microbial biomass production. Geochim Cosmochim Acta 67(20):3871–3887.
   Web of Science ®Google Scholar
• Bethke CM, Johnson TM. 2008. Groundwater age amd groundwater age dating. Annu Rev Earth Planet Sci 36(1):121–152.
   Web of Science ®Google Scholar
• Bleeker W, Parrish RR, Sager-Kinsman A. 1999.  A 2.7 Ga komatiite, lois ti tholeiite transition, and inferred proto-arc proto-arc to arc transition and the geodynamic setting of the Kidd Creek deposit: evidence from precise trace element data.  In: Hannington, MD, Barrie, CT, editors. The Giant Kidd Creek Volcanogenic Massive Sulfide Deposit, Western Abitibi Subprovince, Canada, Vol. Economic Geology Monograph 10. Littleton, U.S.A.: Economic Geology Publishing Co., p 43–69.
   Google Scholar
• Blodgett R. 2010. Most Probable Number from Serial Dilutions. U.S. Food and Drug Administration. Bacterial Analytical Manual. Appendix 2.
   Google Scholar
• Bomberg M, Lamminmäki T, Itävaara M. 2016. Microbial communities and their predicted metabolic characteristics in deep fracture groundwaters of the crystalline bedrock at Olkiluoto, Finland. Biogeosci Discuss 13(21):6031–6047.
   Web of Science ®Google Scholar
• Bomberg M, Nyyssönen M, Pitkänen P, Lehtinen A, Itävaara M. 2015. Active microbial communities inhabit sulphate-methane interphase in deep bedrock fracture fluids in Olkiluoto, Finland. BioMed Res Int 2015:979530.
   PubMed Web of Science ®Google Scholar
• Borgonie G, Linage-Alvarez B, Ojo AO, Mundle SOC, Freese LB, Van Rooyen C, Kuloyo O, Albertyn J, Pohl C, Cason ED, et al. 2015. Eukaryotic opportunists rule the deep subsurface biosphere in South Africa. Nat Commun 6(1):8952.
   PubMed Web of Science ®Google Scholar
• Chivian D, Brodie EL, Alm EJ, Culley DE, Dehal PS, DeSantis TZ, Gihring TM, Lapidus A, Lin L-H, Lowry SR. 2008. Environmental genomics reveals a single-species ecosystem deep within Earth. Science 322(5899):275–278.
   PubMed Web of Science ®Google Scholar
• Cowen JP, Giovannoni SJ, Kenig F, Johnson HP, Butterfield D, Rappé MS, Hutnak M, Lam P. 2003. Fluids from aging ocean crust that support microbial life. Science 299(5603):120–123.
   PubMed Web of Science ®Google Scholar
• Craig H. 1961. Isotopic variations in meteoric waters. Science 133(3465):1702–1703.
   PubMed Web of Science ®Google Scholar
• D’Hondt S, Spivack AJ, Pockalny R, Ferdelman TG, Fischer JP, Kallmeyer J, Abrams LJ, Smith DC, Graham D, Hasiuk F, et al. 2009. Subseafloor sedimentary life in the South Pacific Gyre. Proc Natl Acad Sci USA 106(28):11651–11656.
   PubMed Web of Science ®Google Scholar
• Decoster MA, Maddi S, Dutta V, McNamara J. 2010. Microscopy and image analysis of individual and group cell shape changes during apoptosis. Microsc Sci Technol Appl Educ 2:836–843.
   Google Scholar
• Doig F, Sherwood Lollar B, Ferris FG. 1995. Evidence for abundant microbial communities in Canadian Shield groundwaters – an in situ biofilm experiment. Geomicrob J 13(2):91–101.
   Web of Science ®Google Scholar
• Edwards KJ, Becker K, Colwell F. 2012. The deep, dark energy biosphere: intraterrestrial life on Earth. Annu Rev Earth Planet Sci 40(1):551–568.
   Web of Science ®Google Scholar
• Frape SK, Fritz P, McNutt RH. 1984. Water-rock interaction and chemistry of groundwaters from the Canadian Shield. Geochim Cosmochim Acta 48(8):1617–1627.
   Web of Science ®Google Scholar
• Goodwin AM. 1996. Principles of Precambrian Geology. London: Academic Press. 327 pp.
   Google Scholar
• Heard AW, Warr O, Borgonie G, Linage B, Kuloyo O, Fellowes JW, Magnabosco C, Lau MCY, Erasmus M, Cason E, et al. 2018. Origin and ages of fracture fluids in the South African crust. Chemical Geol 283(3–4):287–296. doi.org/10.1016/l.chemgeo.2018.06.001.
   Google Scholar
• Hoehler TM, Jorgensen BB. 2013. Microbial life under extreme energy limitation. Nat Rev Microbiol 11(2):83–94.
   PubMed Web of Science ®Google Scholar
• Holland G, Sherwood Lollar B, Li L, Lacrampe-Couloume G, Slater GF, Ballentine CJ. 2013. Deep fracture fluids isolated in the crust since the Precambrian era. Nature 497(7449):357–360.
   PubMed Web of Science ®Google Scholar
• Itävaara M, Nyyssönen M, Kapanen A, Nousiainen A, Ahonen L, Kukkonen I. 2011. Characterization of bacterial diversity to a depth of 1500 m in the Outokumpu deep borehole, Fennoscandian Shield. FEMS Microbiol Ecol 77(2):295–309.
   PubMed Web of Science ®Google Scholar
• Jannasch HW, Taylor CD. 1984. Deep-sea microbiology. Annu Rev Microbiol 38:487–514.
   PubMed Web of Science ®Google Scholar
• Kallmeyer J, Pockalny R, Adhikari RR, Smith DC, D’Hondt S. 2012. Global distribution of microbial abundance and biomass in subseafloor sediment. Proc Natl Acad Sci 109(40):15976–15977. 10.1073/pnas.1203849109.
   PubMed Web of Science ®Google Scholar
• Kelley DS, Karson JA, Früh-Green GL, Yoerger DR, Shank TM, Butterfield DA, Hayes JM, Schrenk MO, Olson EJ, Proskurowski G, et al. 2005. A serpentinite-hosted ecosystem: the Lost City Hydrothermal Field. Science 307(5714):1428–1434.
   PubMed Web of Science ®Google Scholar
• Kieft TL, Phelps TJ, Fredrickson JK. 2007. Drilling, coring, and sampling subsurface environments. In: Hurst, CJ, editor. Manual of Environmental Microbiology, Third Edition. Washington, DC: ASM Press, p 799–817.
   Google Scholar
• Kieft TL, Walters CC, Higgins MB, Mennito AS, Clewett CFM, Heuer V, Pullin MJ, Hendrickson S, Van Heerden E, Sherwood Lollar B, et al. 2018. Dissolved organic matter compositions in 0.6-3.4 km deep fracture waters, Kaapval Craton, South Africa. Org Geochem 118:116–131.
   Web of Science ®Google Scholar
• Labonté JM, Field EK, Lau MCY, Chivian D, van Heerden E, Wommack KE, Kieft TL, Onstott TC. 2015. Single cell genomics indicates horizontal gene transfer and viral infections in a deep subsurface Firmicutes population. Front Microbiol 6:349.
   PubMed Web of Science ®Google Scholar
• Lau MCY, Kieft TL, Kuloyo O, Linage-Alvarez B, van Heerden E, Lindsay MR, Magnabosco C, Wang W, Wiggins JB, Guo L, et al. 2016. An oligotrophic deep-subsurface community dependent on syntrophy is dominated by sulfur-driven autotrophic denitrifiers. Proc Natl Acad Sci U S A 113(49):E7927–E7936.
   PubMed Web of Science ®Google Scholar
• Lehmann BE, Davis SN, Fabryka-Martin JT. 1993. Atmospheric and subsurface sources of stable and radioactive nuclides used for groundwater dating. Water Resour Res 29(7):2027–2740.
   Web of Science ®Google Scholar
• Li L, Wing BA, Bui TH, McDermott JM, Slater GF, Wei S, Lacrampe-Couloume G, Sherwood Lollar B. 2016. Sulfur mass-independent fractionation in subsurface fracture waters indicates a long-standing sulfur cycle in Precambrian rocks. Nat Commun 7:13252.
   PubMed Web of Science ®Google Scholar
• Lin L-H, Wang P-L, Rumble D, Lippmann-Pipke J, Boice E, Pratt LM, Sherwood Lollar B, Brodie EL, Hazen TC, Andersen GL, et al. 2006. Long-term sustainability of a high-energy, low-diversity crustal biome. Science 314(5798):479–482.
   PubMed Web of Science ®Google Scholar
• Lipp JS, Morono Y, Inagaki F, Hinrichs K-U. 2008. Significant contribution of Archaea to extant biomass in marine subsurface sediments. Nature 454(7207):991–994.
   PubMed Web of Science ®Google Scholar
• Lippmann J, Stute M, Torgersen T, Moser DP, Hall JA, Lin L, Borcsik M, Bellamy RES, Onstott TC. 2003. Dating ultra-deep mine waters with noble gases and 36Cl, Witwatersrand Basin, South Africa. Geochim Cosmochim Acta 67(23):4597–4619.
   Web of Science ®Google Scholar
• Lippmann-Pipke J, Sherwood Lollar B, Niedermann S, Stroncik NA, Naumann R, van Heerden E, Onstott TC. 2011. Neon identifies two billion year old fluid component in the Witwatersrand Basin. Chem Geol 283(3–4):287–296.
   Web of Science ®Google Scholar
• Magnabosco C, Lin L-H, Dong H, Bomberg M, Ghiorse W, Stan-Lotter H, Pedersen K, Kieft TL, van Heerden E, Onstott TC. 2018a. The biomass and biodiversity of the continental subsurface. Nat Rev 11: 707–717.
   Google Scholar
• Magnabosco C, Ryan K, Lau MCY, Kuloyo O, Sherwood Lollar B, Kieft TL, van Heerden E, Onstott TC. 2016. A metagenomic window into carbon metabolism at 3 km depth in Precambrian Continental Crust. Isme J 10(3):730–741.
   PubMed Web of Science ®Google Scholar
• Magnabosco C, Timmers PHA, Lau MCY, Borgonie G, Linage-Alvarez B, Kuloyo O, Alleva R, Kieft TL, Slater GF, van Heerden E, et al. 2018b. Fluctuations in populations of subsurface methane oxidizers in coordination with changes in electron acceptor availability. FEMS Microbiol Ecol 94(7). doi:10.1093/femsec/fiy089.
   Web of Science ®Google Scholar
• McDermott JM, Seewald JS, German CR, Sylva SP. 2015. Pathways for abiotic organic synthesis at submarine hydrothermal fields. Proc Nat Acad Sci 112:7668–7672.
   PubMed Web of Science ®Google Scholar
• McMahon S, Parnell J. 2014. Weighing the deep continental biosphere. FEMS Microbiol Ecol 87(1):113–120.
   PubMed Web of Science ®Google Scholar
• Michalski JR, Onstott TC, Mojzsis SJ, Mustard JF, Chan Q, Niles PB, Stewart Johnson S. 2017. The Martian subsurface as a potential window into the origin of life. Nat Geosci 3, 838–841.
   Google Scholar
• Nakagawa S, Inagaki F, Suzuki S, Steinsbu B, Lever M, Takai K, Engelen B, Sako Y, Wheat C, Horikoshi K. 2006. Microbial community in black rust exposed to hot ridge flank crustal fluids. Appl Environ Microb 72(10):6789–6799.
   PubMed Web of Science ®Google Scholar
• NASEM (National Academies of Sciences, Engineering, and Medicine). 2018. An Astrobiology Science Strategy for the Search for Life in the Universe. London: The National Academies Press.
   Google Scholar
• Oblinger JL, Koburger JA. 1975. Understanding and teaching the most probable number technique. J Milk Food Technol 38(9):540–545.
   Web of Science ®Google Scholar
• Onstott TC. 2016. Deep Life. Princeton: Princeton University Press. 486 pp.
   Google Scholar
• Onstott TC, Lin L-H, Davidson M, Mislowack B, Borcsik M, Hall J, Slater G, Ward J, Sherwood Lollar B, Lippmann-Pipke J, et al. 2006. The origin and age of biogeochemical trends in deep fracture water of the Witwatersrand Basin. South Africa. Geomicrob J 23:369–414.
   Google Scholar
• Orcutt BN, Bach W, Becker K, Fisher AT, Hentscher M, Toner BM, Wheat CG, Edwards KJ. 2011. Colonization of subsurface microbial observatories deployed in young ocean crust. ISME J 5(4):692–703.
   PubMed Web of Science ®Google Scholar
• Osburn MR, LaRowe DE, Momper LM, Amend JP. 2014. Chemolithotrophy in the continental deep subsurface: Sanford Underground Research Facility (SURF), USA. Front Microbiol 5:610.
   PubMed Web of Science ®Google Scholar
• Pedersen K. 2000. Exploration of deep intraterrestrial microbial life: current perspectives. FEMS Microbiol Lett 185(1):9–16.
   PubMed Web of Science ®Google Scholar
• Pedersen K, Bengtsson AF, Edlund JS, Eriksson LC. 2014. Sulphate-controlled diversity of subterranean microbial communities over depth in deep groundwater with opposing gradients of sulphate and methane. Geomicrob J 31(7):617–631.
   Web of Science ®Google Scholar
• Proskurowski G, Lilley MD, Seewald JS, Früh-Green GL, Olson EJ, Lupton JE, Sylva SP, Kelley DS. 2008. Abiogenic hydrocarbon production at Lost City Hydrothermal Field. Science 319(5863):604–607.
   PubMed Web of Science ®Google Scholar
• Purkamo L, Bomberg M, Nyyssönen M, Kukkonen I, Ahonen L, Itävaara M. 2015. Heterotrophic communities supplied by ancient organic carbon predominate in deep Fennoscandian bedrock fluids. Microb Ecol 69(2):319–332.
   PubMed Web of Science ®Google Scholar
• Purtschert R, Onstott TC, Jiang W, Lu Z-T, Muller P, Zappala J, Yokochi R, van Heerden E, Cason M, Lau M, et al. 2017. Underground production of 81Kr detected in subsurface fluids. Goldschmidt Annual Meeting 2017, Paris, France, Aug. 13–18, 2017.
   Google Scholar
• Santelli CM, Banerjee N, Bach W, Edwards KJ. 2010. Tapping the subsurface ocean crust biosphere: low biomass and drilling-related contamination calls for improved quality controls. Geomicrobiol J 27(2):158–169.
   Web of Science ®Google Scholar
• Schrenk MO, Brazelton WJ, Lang SQ. 2013. Serpentinization, carbon, and deep life. Rev Mineral Geochem 75(1):575.
   Web of Science ®Google Scholar
• Sheik CS, Reese BK, Twing KI, Sylvan JB, Grim SL, Schrenk MO, Sogin ML, Colwell FS. 2018. Identification and removal of contaminant sequences from ribosomal gene database: lessons from the Census of Deep Life. Front Microbiol 9:840.
   PubMed Web of Science ®Google Scholar
• Sherwood Lollar B. 2010. Far-Field Microbiology Considerations Relevant to a Deep Geologic Repository – State of Science Review. NWMO-TR-2010-23. Nuclear Waste Management Organization. 65 p.
   Google Scholar
• Sherwood Lollar B, Lacrampe-Couloume G, Slater GF, Ward J, Moser DP, Gihring TM, Lin L-H, Onstott TC. 2006. Unravelling abiogenic and biogenic sources of methane in the Earth’s deep subsurface. Chem Geol 226(3–4):328–339.
   Web of Science ®Google Scholar
• Sherwood Lollar B, Onstott TC, Lacrampe-Couloume G, Ballentine CJ. 2014. The contribution of the Precambrian continental lithosphere to global H2 production. Nature 516(7531):379–382.
   PubMed Web of Science ®Google Scholar
• Sherwood Lollar B, Westgate T, Ward J, Slater GF, Lacrampe-Couloume G. 2002. Abiogenic formation of alkanes in the Earth’s crust as a minor source for global hydrocarbon reservoirs. Nature 416(6880):522–524.
   PubMed Web of Science ®Google Scholar
• Simkus DN, Slater GF, Sherwood Lollar B, Wilkie K, Kieft TL, Magnabosco C, Lau MCY, Pullin MJ, Hendrickson SB, Wommack KE, et al. 2016. Variations in microbial carbon sources and cycling in the deep continental subsurface. Geochim Cosmochim Acta 173:264–283.
   Web of Science ®Google Scholar
• Smith PE, Schandl ES, York D. 1993. Timing of metasomatic alteration of the Archean Kidd Creek massive sulfide deposit, Ontario, using 40Ar–39Ar laser dating of single crystals of fuchsite. Econ Geol 88(6):1636–1642.
   Web of Science ®Google Scholar
• Telling J, Voglesonger K, Sherwood Lollar B, Sutcliffe NC, Lacrampe-Couloume G, Edwards EA. 2018. Bioenergetic constraints on microbial hydrogen utilization in Precambrian deep crustal fracture fluids. Geomicrob J 35(2):108–119.
   Web of Science ®Google Scholar
• Thurston PC, Ayer JA, Goutier J, Hamilton MA. 2008. Depositional gaps in Abitibi greenstone belt stratigraphy: a key to exploration for syngenetic mineralization. Econ Geol 103(6):1097–1134.
   Web of Science ®Google Scholar
• Waite JH, Glein CR, Perryman RS, Teolis BD, Magee BA, Miller G, Grimes J, Perry ME, Miller KE, Bouquet A, et al. 2017. Cassini finds molecular hydrogen in the Enceladus plume: evidence for hydrothermal processes. Science 356(6334):155–159.
   PubMed Web of Science ®Google Scholar
• Ward J, Slater GF, Moser D, Lin L, Lacrampe-Couloume G, Bonin AS, Davidson M, Hall JA, Mislowack B, Bellamy RES, et al. 2004. Microbial hydrocarbon gases in the Witwatersrand Basin, South Africa: implications for the deep biosphere. Geochim Cosmo Acta 68(15):3239–3250.
   Web of Science ®Google Scholar
• Warr O, Sherwood Lollar B, Fellowes J, Sutcliffe NC, McDermott J, Holland G, Mabry JC, Ballentine CJ. 2018. Tracing ancient hydrogeological fracture network age and compartmentalisation using noble gases. Geochim Cosmochim Acta 222:340–362.
   Web of Science ®Google Scholar
• Young ED, Kohl IE, Sherwood Lollar B, Etiope G, Rumble D, Li S, Haghnegahdar MA, Schauble EA, McCain KA, Foustoukos DI, et al. 2017. The relative abundances of resolved 12CH2D2 and 13CH3D and mechanisms controlling isotopic bond ordering in abiotic and biotic methane gases. Geochim Cosmochim Acta 203:235–264.
   Web of Science ®Google Scholar