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Review

Metagenomic Bioprospecting of Uncultivable Microbial Flora in Soil Microbiome for Novel Enzymes

, & ORCID Icon
Pages 97-106 | Received 26 Mar 2021, Accepted 03 Dec 2021, Published online: 26 Dec 2021

References

  • Aakvik T, Degnes KF, Dahlsrud R, Schmidt F, Dam R, Yu L, Volker U, Ellingsen TE, Valla S. 2009. A plasmid RK2-based broad-host-range cloning vector useful for transfer of metagenomic libraries to a variety of bacterial species. FEMS Microbiol Lett. 296:149–158.
  • Antelo V, Giménez M, Azziz G, Valdespino‐Castillo P, Falcón LI, Ruberto LAM, Mac Cormack WP, Mazel D, Batista S. 2021. Metagenomic strategies identify diverse integron‐integrase and antibiotic resistance genes in the Antarctic environment. MicrobiologyOpen. 10:219.
  • Arjun JK, Aneesh BP, Kavitha T, Harikrishnan K. 2018. Characterization of a novel asparaginase from soil metagenomic libraries generated from forest soil. Biotechnol Lett. 40:343–348.
  • Ausec L, Berini F, Casciello C, Cretoiu MS, van Elsas JD, Marinelli F, Mandic-Mulec I. 2017. The first acidobacterial laccase-like multicopper oxidase revealed by metagenomics shows high salt and thermo-tolerance. Appl Microbiol Biotechnol. 101:6261–6276.
  • Bayer TS, Widmaier DM, Temme K, Mirsky EA, Santi DV, Voigt CA. 2009. Synthesis of methyl halides from biomass using engineered microbes. J Am Chem Soc. 131:6508–6515.
  • Bernstein JR, Bulter T, Shen CR, Liao JC. 2007. Directed evolution of ribosomal protein S1 for enhanced translational efficiency of high GC Rhodopseudomonas palustris DNA in Escherichia coli. J Biol Chem. 282:18929–18936.
  • Berry AE, Chiocchini C, Selby T, Sosio M, Wellington EMH. 2003. Isolation of high molecular weight DNA from soil for cloning into BAC vectors. FEMS Microbiol Lett. 223:15–20.
  • Bertrand H, Poly F, Van VT, Lombard N, Nalin R, Vogel TM, Simonet P. 2005. High molecular weight DNA recovery from soils prerequisite for biotechnological metagenomic library construction. J Microbiol Methods. 62:1–11.
  • Biver S, Stroobants A, Portetelle D, Vandenbol M. 2014. Two promising alkaline β-glucosidases isolated by functional metagenomics from agricultural soil, including one showing high tolerance towards harsh detergents, oxidants and glucose. J Ind Microbiol Biotechnol. 41:479–488.
  • Brady SF, Chao CJ, Clardy J. 2002. New natural product families from an environmental DNA (eDNA) gene cluster. J Am Chem Soc. 124:9968–9969.
  • Chae JC, Song B, Zylstra GJ. 2008. Identification of genes coding for hydrolytic dehalogenation in the metagenome derived from a denitrifying 4-chlorobenzoate degrading consortium. FEMS Microbiol Lett. 281:203–209.
  • Chen ZW, Liu YY, Wu JF, She Q, Jiang CY, Liu SJ. 2007. Novel bacterial sulfur oxygenase reductases from bioreactors treating gold-bearing concentrates. Appl Microbiol Biotechnol. 74:688–698.
  • Connon SA, Giovannoni SJ. 2002. High-throughput methods for culturing microorganisms in very-low-nutrient media yield diverse new marine isolates. Appl Environ Microbiol. 68:3878–3885.
  • Courtois S, Cappellano CM, Ball M, Francou FX, Normand P, Helynck G, Martinez A, Kolvek SJ, Hopke J, Osburne MS, et al. 2003. Recombinant environmental libraries provide access to microbial diversity for drug discovery from natural products. Appl Environ Microbiol. 69:49–55.
  • Cullen DW, Hirsch PR. 1998. Simple and rapid method for direct extraction of microbial DNA from soil for PCR. Soil Biol Biochem. 30:983–993.
  • Curtis TP, Sloan WT. 2005. Exploring microbial diversity – a vast below. Science. 309:1331–1333.
  • Daniel R. 2002. Construction of environmental libraries for functional screening of enzyme activity. In: J.K.W. Brakmann S, editor. Directed molecular evolution of proteins. Germany: Wiley-VCH Verlag GmbH.
  • Daniel R. 2004. The soil metagenome-a rich resource for the discovery of novel natural products. Curr Opin Biotechnol. 15:199–204.
  • Das G, Dineshkumar TK, Thanedar S, Varshney U. 2005. Acquisition of a stable mutation in metY allows efficient initiation from an amber codon in Escherichia coli. Microbiology. 151:1741–1750.
  • Demanèche S, Philippot L, David MM, Navarro E, Vogel TM, Simonet P. 2009. Characterization of denitrification gene clusters of soil bacteria via a metagenomic approach. Appl Environ Microbiol. 75:534–537.
  • Denef VJ, Park J, Rodrigues JLM, Tsoi TV, Hashsham SA, Tiedje JM. 2003. Validation of a more sensitive method for using spotted oligonucleotide DNA microarrays for functional genomics studies on bacterial communities. Environ Microbiol. 5:933–943.
  • Doolittle WF. 1999. Phylogenetic classification and the universal tree. Science. 284:2124–2129.
  • Elsaied H, Stokes HW, Nakamura T, Kitamura K, Fuse H, Maruyama A. 2007. Novel and diverse integron integrase genes and integron-like gene cassettes are prevalent in deep-sea hydrothermal vents. Environ Microbiol. 9:2298–2312.
  • Epstein SS, Lewis K, Nichols D, Gavrish E. 2014. New approaches to microbial isolation. Manual Ind Microbiol Biotechnol. 2014:ch1.
  • Ferrer M, Chernikova TN, Timmis KN, Golyshin PN. 2004. Expression of a temperature-sensitive esterase in a novel chaperone-based Escherichia coli strain. Appl Environ Microbiol. 70:4499–4504.
  • Fieseler L, Hentschel U, Grozdanov L, Schirmer A, Wen G, Platzer M, Hrvatin S, Butzke D, Zimmermann K, Piel J. 2007. Widespread occurrence and genomic context of unusually small polyketide synthase genes in microbial consortia associated with marine sponges. Appl Environ Microbiol. 73:2144–2155.
  • Fisch KM, Gurgui C, Heycke N, Van Der Sar SA, Anderson SA, Webb VL, Taudien S, Platzer M, Rubio BK, Robinson SJ, et al. 2009. Polyketide assembly lines of uncultivated sponge symbionts from structure-based gene targeting. Nat Chem Biol. 5:494–501.
  • Gans J, Wolinsky M, Dunbar J. 2005. Microbiology: computational improvements reveal great bacterial diversity and high toxicity in soil. Science. 309:1387–1390.
  • Gloux K, Leclerc M, Iliozer H, L'Haridon R, Manichanh C, Corthier G, Nalin R, Blottière HM, Doré Jl, 2007. Development of high-throughput phenotyping of metagenomic clones from the human gut microbiome for modulation of eukaryotic cell growth. Appl Environ Microbiol. 73:3734–3737.
  • Gomes-Pepe ES, Sierra EGM, Pereira MR, Castellane TCL, De Lemos EGM. 2016. Bg10: a novel metagenomics alcohol-tolerant and glucose-stimulated gh1 ß-Glucosidase Suitable for Lactose-Free Milk Preparation. PLoS One. 11:e0167932.
  • Guan C, Ju J, Borlee BR, Williamson LL, Shen B, Raffa KF, Handelsman J. 2007. Signal mimics derived from a metagenomic analysis of the gypsy moth gut microbiota. Appl Environ Microbiol. 73:3669–3676.
  • Handelsman J, Rondon MR, Brady SF, Clardy J, Goodman RM. 1998. Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem Biol. 5:R245–R249.
  • Henne A, Schmitz RA, Bömeke M, Gottschalk G, Daniel R. 2000. Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on Escherichia coli. Appl Environ Microbiol. 66:3113–3116.
  • Hosaka T, Ohnishi-Kameyama M, Muramatsu H, Murakami K, Tsurumi Y, Kodani S, Yoshida M, Fujie A, Ochi K. 2009. Antibacterial discovery in actinomycetes strains with mutations in RNA polymerase or ribosomal protein S12. Nat Biotechnol. 27:462–464.
  • Hu Y, Zhang G, Li A, Chen J, Ma L. 2008. Cloning and enzymatic characterization of a xylanase gene from a soil-derived metagenomic library with an efficient approach. Appl Microbiol Biotechnol. 80(5):823–830. doi:https://doi.org/10.1007/s00253-008-1636-6.
  • Hurt RA, Qiu X, Wu L, Roh Y, Palumbo AV, Tiedje JM, Zhou J. 2001. Simultaneous recovery of RNA and DNA from soils and sediments. Appl Environ Microbiol. 67:4495–4503.
  • Janssen PH, Yates PS, Grinton BE, Taylor PM, Sait M. 2002. Improved culturability of soil bacteria and isolation in pure culture of novel members of the divisions Acidobacteria, Actinobacteria, Proteobacteria, and Verrucomicrobia. Appl Environ Microbiol. 68:2391–2396.
  • Jeon JH, Kim J-T, Kim YJ, Kim H-K, Lee HS, Kang SG, Kim S-J, Lee J-H. 2009. Cloning and characterization of a new cold-active lipase from a deep-sea sediment metagenome. Appl Microbiol Biotechnol. 81(5):865–874. doi:https://doi.org/10.1007/s00253-008-1656-2.
  • Joseph SJ, Hugenholtz P, Sangwan P, Osborne CA, Janssen PH. 2003. Laboratory cultivation of widespread and previously uncultured soil bacteria. Appl Environ Microbiol. 69:7210–7215.
  • Kaeberlein T, Lewis K, Epstein SS. 2002. Isolating ‘uncultivabte’ microorganisms in pure culture in a simulated natural environment. Science. 296:1127–1129.
  • Kazimierczak KA, Scott KP, Kelly D, Aminov RI. 2009. Tetracycline resistome of the organic pig gut. Appl Environ Microbiol. 75:1717–1722.
  • Kéki Z, Grébner K, Bohus V, Márialigeti K, Tóth E. 2013. Application of special oligotrophic media for cultivation of bacterial communities originated from ultrapure water. Acta Microbiol Immunol Hung. 60:345–357.
  • Kellenberger E. 2001. Exploring the unknown. The silent revolution of microbiology. EMBO Rep. 2:5–7.
  • Kim E-Y, Oh K-H, Lee M-H, Kang C-H, Oh T-K, Yoon J-H. 2009. Novel cold-adapted alkaline lipase from an intertidal flat metagenome and proposal for a new family of bacterial lipases. Appl Environ Microbiol. 75(1):257–260. doi:https://doi.org/10.1128/AEM.01400-08.
  • Knietsch A, Bowien S, Whited G, Gottschalk G, Daniel R. 2003. Identification and characterization of coenzyme B12-dependent glycerol dehydratase- and diol dehydratase-encoding genes from metagenomic DNA libraries derived from enrichment cultures. Appl Environ Microbiol. 69:3048–3060.
  • Knietsch A, Waschkowitz T, Bowien S, Henne A, Daniel R. 2003a. Construction and screening of metagenomic libraries derived from enrichment cultures: generation of a gene bank for genes conferring alcohol oxidoreductase activity on Escherichia coli. Appl Environ Microbiol. 69:1408–1416.
  • Knietsch A, Waschkowitz T, Bowien S, Henne A, Daniel R. 2003b. Metagenomes of complex microbial consortia derived from different soils as sources for novel genes conferring formation of carbonyls from short-chain polyols on Escherichia coli. J Mol Microbiol Biotechnol. 5:46–56.
  • Koenig JE, Sharp C, Dlutek M, Curtis B, Joss M, Boucher Y, Doolittle WF. 2009. Integron gene cassettes and degradation of compounds associated with industrial waste: the case of the Sydney tar ponds. PLoS One. 4:e5276.
  • Lam KN, Cheng J, Engel K, Neufeld JD, Charles TC. 2015. Current and future resources for functional metagenomics. Front Microbiol. 6:1196.
  • Lämmle K, Zipper H, Breuer M, Hauer B, Buta C, Brunner H, Rupp S. 2007. Identification of novel enzymes with different hydrolytic activities by metagenome expression cloning. J Biotechnol. 127:575–592.
  • Lee C-M, Yeo Y-S, Lee J-H, Kim S-J, Kim J-B, Han NS, Koo B-S, Yoon S-H. 2008. Identification of a novel 4-hydroxyphenylpyruvate dioxygenase from the soil metagenome. Biochem. Biophys. Res. Commun. 370(2):322–326. doi:https://doi.org/10.1016/j.bbrc.2008.03.102.
  • Li Y, Wexler M, Richardson DJ, Bond PL, Johnston AWB. 2005. Screening a wide host-range, waste-water metagenomic library in tryptophan auxotrophs of Rhizobium leguminosarum and of Escherichia coli reveals different classes of cloned trp genes. Environ Microbiol. 7:1927–1936.
  • Liles MR, Manske BF, Bintrim SB, Handelsman J, Goodman RM. 2003. A census of rRNA genes and linked genomic sequences within a soil metagenomic library. Appl Environ Microbiol. 69:2684–2691.
  • Liu P, Wang W, Zhao J, Wei D. 2019. Screening novel β-galactosidases from a sequence-based metagenome and characterization of an alkaline β-galactosidase for the enzymatic synthesis of galactooligosaccharides. Protein Expr Purif. 155:104–111.
  • Lloyd-Jones G, Hunter DWF. 2001. Comparison of rapid DNA extraction methods applied to contrasting New Zealand soils. Soil Biol Biochem. 33:2053–2059.
  • Lombard N, Prestat E, van Elsas JD, Simonet P. 2011. Soil-specific limitations for access and analysis of soil microbial communities by metagenomics. FEMS Microbiol Ecol. 78:31–49.
  • Maar D, Liveris D, Sussman JK, Ringquist S, Moll I, Heredia N, Kil A, Bläsi U, Schwartz I, Simons RW. 2008. A single mutation in the IF3 N-terminal domain perturbs the fidelity of translation initiation at three levels. J Mol Biol. 383:937–944.
  • Majerník A, Gottschalk G, Daniel R. 2001. Screening of environmental DNA libraries for the presence of genes conferring Na(+)(Li(+))/H(+) antiporter activity on Escherichia coli: characterization of the recovered genes and the corresponding gene products. J Bacteriol. 183:6645–6653.
  • Maruthamuthu M, Jiménez DJ, Stevens P, van Elsas JD. 2016. A multi-substrate approach for functional metagenomics-based screening for (hemi)cellulases in two wheat straw-degrading microbial consortia unveils novel thermoalkaliphilic enzymes. BMC Genomics. 17:86.
  • Maruthamuthu M, Jiménez DJ, van Elsas JD. 2017. Characterization of a furan aldehyde-tolerant β-xylosidase/α-arabinosidase obtained through a synthetic metagenomics approach. J Appl Microbiol. 123:145–158.
  • Marzorati M, De Ferra F, Van Raemdonck H, Borin S, Allifranchini E, Carpani G, Serbolisca L, Verstraete W, Boon N, Daffonchio D. 2007. A novel reductive dehalogenase, identified in a contaminated groundwater enrichment culture and in Desulfitobacterium dichloroeliminans strain DCA1, is linked to dehalogenation of 1,2-dichloroethane. Appl Environ Microbiol. 73:2990–2999.
  • Mayumi D, Akutsu-Shigeno Y, Uchiyama H, Nomura N, Nakajima-Kambe T. 2008. Identification and characterization of novel poly(DL-lactic acid) depolymerases from metagenome. Appl Microbiol Biotechnol. 79:743–750.
  • Mirete S, De Figueras CG, González-Pastor JE. 2007. Novel nickel resistance genes from the rhizosphere metagenome of plants adapted to acid mine drainage. Appl Environ Microbiol. 73:6001–6011.
  • Mohn WW, Garmendia J, Galvao TC, De Lorenzo V. 2006. Surveying biotransformations with à la carte genetic traps: translating dehydrochlorination of lindane (gamma-hexachlorocyclohexane) into lacZ-based phenotypes. Environ Microbiol. 8:546–555.
  • Mori T, Mizuta S, Suenaga H, Miyazaki K. 2008. Metagenomic screening for bleomycin resistance genes. Appl Environ Microbiol. 74:6803–6805.
  • Morimoto S, Fujii T. 2009. A new approach to retrieve full lengths of functional genes from soil by PCR-DGGE and metagenome walking. Appl Microbiol Biotechnol. 83:389–396.
  • Mtimka S, Pillay P, Rashamuse K, Gildenhuys S, Tsekoa TL. 2020. Functional screening of a soil metagenome for DNA endonucleases by acquired resistance to bacteriophage infection. Mol Biol Rep. 47:353–361.
  • Nagayama H, Sugawara T, Endo R, Ono A, Kato H, Ohtsubo Y, Nagata Y, Tsuda M. 2015. Isolation of oxygenase genes for indigo-forming activity from an artificially polluted soil metagenome by functional screening using Pseudomonas putida strains as hosts. Appl Microbiol Biotechnol. 99:4453–4470.
  • Nascimento Lemos L, Manoharan L, William Mendes L, Monteiro Venturini A, Satler Pylro V, Tsai SM. 2020. Metagenome assembled-genomes reveal similar functional profiles of CPR/Patescibacteria phyla in soils. Environ Microbiol Rep. 12:651–655.
  • Ngara TR, Zhang H. 2018. Recent advances in function-based metagenomic screening. Genomics Proteomics Bioinformatics. 16:405–415.
  • Nunan N, Wu K, Young IM, Crawford JW, Ritz K. 2003. Spatial distribution of bacterial communities and their relationships with the micro-architecture of soil. FEMS Microbiol. Ecology. 44:203–215.
  • O’Connor M, Gregory ST, Dahlberg AE. 2004. Multiple defects in translation associated with altered ribosomal protein L4. Nucleic Acids Res. 32:5750–5756.
  • Ono A, Miyazaki R, Sota M, Ohtsubo Y, Nagata Y, Tsuda M. 2007. Isolation and characterization of naphthalene-catabolic genes and plasmids from oil-contaminated soil by using two cultivation-independent approaches. Appl Microbiol Biotechnol. 74:501–510.
  • Osburne TG, Pr A, Ia M. 2000. Tapping into microbial diversity for natural products drug discovery. ASM News. 66:411–417.
  • Pham VH, Kim J. 2012. Cultivation of unculturable soil bacteria. Trends Biotechnol. 30:475–484.
  • Quaiser A, Ochsenreiter T, Klenk HP, Kletzin A, Treusch AH, Meurer G, Eck J, Sensen CW, Schleper C. 2002. First insight into the genome of an uncultivated crenarchaeote from soil. Environ Microbiol. 4:603–611.
  • Quaiser A, Ochsenreiter T, Lanz C, Schuster SC, Treusch AH, Eck J, Schleper C. 2003. Acidobacteria form a coherent but highly diverse group within the bacterial domain: evidence from environmental genomics. Mol Microbiol. 50:563–575.
  • Richardson TH, Tan X, Frey G, Callen W, Cabell M, Lam D, Macomber J, Short JM, Robertson DE, Miller C. 2002. A novel, high performance enzyme for starch liquefaction. Discovery and optimization of a low pH, thermostable α-amylase. J Biol Chem. 277:26501–26507.
  • Rondon MR, August PR, Bettermann AD, Brady SF, Grossman TH, Liles MR, Loiacono KA, Lynch BA, MacNeil IA, Minor C, et al. 2000. Cloning the soil metagenome: a strategy for accessing the genetic and functional diversity of uncultured microorganisms. Appl Environ Microbiol. 66:2541–2547.
  • Santosa DA. 2001. Rapid extraction and purification of environmental DNA for molecular cloning applications and molecular diversity studies. Mol Biotechnol. 17:59–64.
  • Schipper C, Hornung C, Bijtenhoorn P, Quitschau M, Grond S, Streit WR. 2009. Metagenome-derived clones encoding two novel lactonase family proteins involved in biofilm inhibition in pseudomonas aeruginosa. Appl Environ Microbiol. 75:224–233.
  • Schloss PD, Handelsman J. 2003. Biotechnological prospects from metagenomics. Curr Opin Biotechnol. 14:303–310.
  • Sebat JL, Colwell FS, Crawford RL. 2003. Metagenomic profiling: microarray analysis of an environmental genomic library. Appl Environ Microbiol. 69:4927–4934.
  • Shah RK, Patel AK, Davla DM, Parikh IK, Subramanian RB, Patel KC, Jakhesara SJ, Joshi CG. 2017. Molecular cloning, heterologous expression, and functional characterization of a cellulolytic enzyme (Cel PRII) from buffalo rumen metagenome. 3 Biotech. 7:257.
  • Simon C, Herath J, Rockstroh S, Daniel R. 2009. Rapid identification of genes encoding DNA polymerases by function-based screening of metagenomic libraries derived from glacial ice. Appl Environ Microbiol. 75:2964–2968.
  • Skinner FA, Jones PC, Mollison JE. 1952. A comparison of a direct- and a plate counting technique for the quantitative estimation of soil micro-organisms. J Gen Microbiol. 6:261–271.
  • Soares F, Marcon J, Pereira e Silva M, Khakhum N, Cerdeira L, Ottoni J, Domingos D, Taketani R, de Oliveira V, Lima A, et al. 2017. A novel multifunctional β-N-acetylhexosaminidase revealed through metagenomics of an oil-spilled mangrove. Bioengineering. 4:62.
  • Sørensen J. 1997. The rhizosphere as a habitat for soil microorganisms. In van Elsas JD, Trevors JT, and Wellington EMH, editors. Modern Soil Microbiology. New York: Marcel Dekker, p21–45.
  • Stockdale EA, Brookes PC. 2006. Detection and quantification of the soil microbial biomass – impacts on the management of agricultural soils. J Agric Sci. 144:285–302.
  • Stokes HW, Holmes AJ, Nield BS, Holley MP, Nevalainen KMH, Mabbutt BC, Gillings MR. 2001. Gene cassette PCR: sequence-independent recovery of entire genes from environmental DNA. Appl Environ Microbiol. 67:5240–5246.
  • Strohl WR. 2000. The role of natural products in a modern drug discovery program. Drug Discov Today. 5:39–41.
  • Suenaga H, Ohnuki T, Miyazaki K. 2007. Functional screening of a metagenomic library for genes involved in microbial degradation of aromatic compounds. Environ Microbiol. 9:2289–2297.
  • Tang K, Kobayashi RS, Champreda V, Eurwilaichitr L, Tanapongpipat S. 2008. Isolation and characterization of a novel thermostable neopullulanase-like enzyme from a hot spring in Thailand. Biosci Biotechnol Biochem. 72:1448–1456.
  • Uchiyama T, Abe T, Ikemura T, Watanabe K. 2005. Substrate-induced gene-expression screening of environmental metagenome libraries for isolation of catabolic genes. Nat Biotechnol. 23:88–93.
  • Uchiyama T, Miyazaki K. 2009. Functional metagenomics for enzyme discovery: challenges to efficient screening. Curr Opin Biotechnol. 20:616–622.
  • Uchiyama T, Watanabe K. 2006. Improved inverse PCR scheme for metagenome walking. Biotechniques. 41:183–188.
  • Uchiyama T, Watanabe K. 2008. Substrate-induced gene expression (SIGEX) screening of metagenome libraries. Nat Protoc. 3:1202–1212.
  • Van Hellemond EW, Janssen DB, Fraaije MW. 2007. Discovery of a novel styrene monooxygenase originating from the metagenome. Appl Environ Microbiol. 73(18):5832–5839. doi:https://doi.org/10.1128/AEM.02708-06.
  • Van Sint Fiet S, Van Beilen JB, Witholt B. 2006. Selection of biocatalysts for chemical synthesis. Proc Natl Acad Sci USA. 103:1693–1698.
  • Verma SK, Sharma PC. 2021. Isolation and biochemical characterization of a novel serine protease identified from solid tannery waste metagenome. Biologia. 76:3163–3174.
  • Vester JK, Glaring MA, Stougaard P. 2014. Discovery of novel enzymes with industrial potential from a cold and alkaline environment by a combination of functional metagenomics and culturing. Microb Cell Fact. 13:72.
  • Voget S, Leggewie C, Uesbeck A, Raasch C, Jaeger KE, Streit WR. 2003. Prospecting for Novel biocatalysts in a soil metagenome. Appl Environ Microbiol. 69:6235–6242.
  • Wang GYS, Graziani E, Waters B, Pan W, Li X, McDermott J, Meurer G, Saxena G, Andersen RJ, Davies J. 2000. Novel natural products from soil DNA libraries in a streptomycete host. Org Lett. 2:2401–2404.
  • Wang C, Meek DJ, Panchal P, Boruvka N, Archibald FS, Driscoll BT, Charles TC. 2006. Isolation of poly-3-hydroxybutyrate metabolism genes from complex microbial communities by phenotypic complementation of bacterial mutants. Appl Environ Microbiol. 72:384–391.
  • Waschkowitz T, Rockstroh S, Daniel R. 2009. Isolation and characterization of metalloproteases with a novel domain structure by construction and screening of metagenomic libraries. Appl Environ Microbiol. 75(8):2506–2516. doi:https://doi.org/10.1128/AEM.02136-08.
  • Wery N, Gerike U, Sharman A, Chaudhuri JB, Hough DW, Danson MJ. 2003. Use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from Antarctic soil. Appl Environ Microbiol. 69:1457–1464.
  • Wu L, Thompson DK, Li G, Hurt RA, Tiedje JM, Zhou J. 2001. Development and evaluation of functional gene arrays for detection of selected genes in the environment. Appl Environ Microbiol. 67:5780–5790.
  • Xu M, Xiao X, Wang F. 2008. Isolation and characterization of alkane hydroxylases from a metagenomic library of Pacific deep-sea sediment. Extremophiles. 12:255–262.
  • Yamada K, Terahara T, Kurata S, Yokomaku T, Tsuneda S, Harayama S. 2008. Retrieval of entire genes from environmental DNA by inverse PCR with pre-amplification of target genes using primers containing locked nucleic acids. Environ Microbiol. 10:978–987.
  • Yang C, Xia Y, Qu H, Li AD, Liu R, Wang Y, Zhang T. 2016. Discovery of new cellulases from the metagenome by a metagenomics-guided strategy. Biotechnol Biofuels. 9:138.
  • Zengler K, Toledo G, Rappé M, Elkins J, Mathur EJ, Short JM, Keller M. 2002. Cultivating the uncultured. Proc Natl Acad Sci USA. 99:15681–15686.
  • Zhao X, Liu L, Deng Z, Liu S, Yun J, Xiao X, Li H. 2021. Screening, cloning, enzymatic properties of a novel thermostable cellulase enzyme, and its potential application on water hyacinth utilization. Int Microbiol. 24:337–349.

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