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Original Articles

Coupled Application of Aqueous Two-Phase Partitioning and 2D-Electrophoresis for Characterization of Soybean Proteins

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Pages 2210-2225 | Received 12 Feb 2010, Accepted 16 Apr 2010, Published online: 24 Nov 2010

REFERENCES

  • Twyman , R.M. ; Stoger , E. ; Schillberg , S. ; Christou , P. ; Fischer , R. ( 2003 ) Molecular farming in plants: host systems and expression technology . Trends in Biotechnol. , 21 : 570 .
  • Larrick , J.W. ; Yu , L. ; Naftzger , C. ; Jaiswal , S. ; Wycoff , K. ( 2001 ) Production of secretory IgA antibodies in plants . Biomol. Eng. , 18 : 87 .
  • Evangelista , R.L. ; Kusnadi , A.R. ; Howard , J.A. ; Nikolov , Z.L. ( 1998 ) Process and economic evaluation of the extraction and purification of recombinant beta-glucuronidase from transgenic corn . Biotechnol. Prog. , 14 : 607 .
  • Benavides , J. ; Mena , J. ; Cisneros , M. ; Ramirez , O.T. ; Palomares , L.A. ; Rito-Palomares , M. ( 2006 ) Rotavirus-like particles primary recovery from the insect cell in aqueous two-phase systems . J. Chromatogr. B , 842 : 48 .
  • Menkhaus , T.J. ; Glatz , C.E. ( 2005 ) Antibody capture from corn endosperm extracts by packed bed and expanded bed adsorption . Biotechnol. Prog. , 21 : 473 .
  • Albertsson , P.A. ( 1958 ) Partition of proteins in liquid polymer-polymer two-phase systems . Nature , 182 : 709 .
  • Platis , D. ; Labrou , N.E. ( 2009 ) Application of a PEG/salt aqueous two-phase partition system for the recovery of monoclonal antibodies from unclarified transgenic tobacco extract . Biotechnol. J. , 4 ( 9 ): 1320 .
  • Gu , Z. ; Glatz , C.E. ( 2007 ) Aqueous two-phase extraction for protein recovery from corn extracts . J. Chromatogr. B , 845 : 38 .
  • Rito-Palomares , M. ( 2004 ) Practical application of aqueous two-phase partition to process development for the recovery of biological products . J. Chromatogr. B , 80 : 3 .
  • Balasubramaniam , D. ; Wilkinson , C. ; Cott , K.V. ; Zhang , C. ( 2003 ) Tobacco protein separation by aqueous two-phase extraction . J. Chromatogr. A , 989 : 119 .
  • da Silva , M.E. ; Franco , T.T. ( 2000 ) Purification of soybean peroxidase (Glycine max) by metal affinity partitioning in aqueous two-phase systems . J. Chromatogr. B , 743 : 287 .
  • Truust , H. ; Johansson , G. ( 1996 ) Fractionation of wheat proteins by counter-current distribution using aqueous two-phase systems containing propionic acid . J. Chromatogr. B , 680 : 71 .
  • Hachem , F. ; Andrews , B.A. ; Asenjo , J.A. ( 1996 ) Hydrophobic partitioning of proteins in aqueous two-phase systems . Enzyme Microb. Tech. , 19 : 507 .
  • Gu , Z. ; Glatz , C.E. ( 2007 ) A method for three-dimensional protein characterization and its application to a complex plant (corn) extract . Biotechnol. Bioeng. , 97 : 1158 .
  • Asenjo , J.A. ; Andrews , B.A. ( 2004 ) Is there a rational method to purify proteins? From expert systems to proteomics . J. Mol. Recognit. , 17 : 236 .
  • Görg , A. ; Weiss , W. ; Dunn , M.J. ( 2004 ) Current two-dimensional electrophoresis technology for proteomics . Proteomics , 4 : 3665 .
  • Natarajan , S.S. ; Xu , C. ; Hanhong , B. ; Caperna , T.J. ; Garret , W.M. ( 2006 ) Characterization of storage proteins in wild (Glycine soja) and cultivated (Glycine max) soybean seeds using proteomic analysis . J. Agric. Food Chem. , 54 : 3114 .
  • Salgado , J.C. ; Rapaport , I. ; Asenjo , J.A. ( 2005 ) Is it possible to predict the average surface hydrophobicity of a protein using only its amino acid composition? . J. Chromatogr. A , 1075 : 133 .
  • Andrews , B.A. ; Schmidt , A.S. ; Asenjo , J.A. ( 2005 ) Correlation for the partition behavior of proteins in aqueous two-phase systems: Effect of surface hydrophobicity and charge . Biotechnol. Bioeng. , 90 : 380 .
  • Franco , T.T. ; Andrews , A.T. ; Asenjo , J.A. ( 1996 ) Use of chemically modified proteins to study the effect of a single protein property on partitioning in aqueous two-phase systems: Effect of surface hydrophobicity . Biotech. Bioeng. , 49 : 300 .
  • Aguilar , O. ; Glatz , C.E. ; Rito-Palomares , M. ( 2009 ) Characterization of green-tissue protein extract from alfalfa (Medicago sativa) exploiting a 3D technique . J. Sep. Sci. , 32 : 3223 .
  • Martinell , B.J. ; Julson , L.S. ; Emler , C.A. ; Yong , H. ; McCabe , D.E. ; Williams , E.J. ( May 7, 2002 ) Soybean Agrobacterium transformation method . U.S. Patent 6,384,301 .
  • Hinchee , M.A. ; Connor-Ward , D. ( October 20, 1998 ) Method for soybean transformation and regeneration . U.S. Patent 5,824,877 .
  • Aguilar , O. ; Rito-Palomares , M. ( 2006 ) Processing of soybeans (Glycine max.) extracts in aqueous two-phase systems as a first step for the potential recovery of recombinant proteins . J. Chem. Technol. Biotechnol. , 83 : 286 .
  • Thanh , V.H. ; Shibasaki , K. ( 1976 ) Major proteins of soybean seeds. A straightforward fractionation and characterization . J. Agric. Food Chem. , 24 : 1117 .
  • Bradford , M.M. ( 1976 ) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding . Anal. Biochem. , 72 : 248 .
  • Zaslavsky , A. ( 1995 ) Aqueous Two-Phase Partitioning. Physical Chemistry and Bioanalytical Applications ; Marcel Dekker Inc. : New York .
  • Yaklich , R.W. ( 2001 ) β-conglycinin and glycinin in high-protein soybean seeds . J. Agric. Food Chem. , 49 : 729 .
  • Gasteiger , E. ; Gattiker , A. ; Hoogland , C. ; Ivanyi , I. ; Appel , R.D. ; Bairoch , A. ( 2003 ) ExPASy: the proteomics server for in-depth protein knowledge and analysis Nucleic Acids Res . 31:3784. http://www.expasy.org/tools/protparam.html
  • Xu , L. ; Glatz , C.E. ( 2009 ) Predicting protein retention time in ion-exchange chromatography based on three-dimensional protein characterizations . J. Chromatogr. A , 1216 : 274 .
  • Rito-Palomares , M. ; Negrete , A. ; Miranda , L. ; Flores , C. ; Galindo , E. ; Serrano-Carreón , L. ( 2001 ) The potential application of aqueous two-phase systems for in situ recovery of 6-pentyl--pyrone produced by Trichoderma harzianum . Enzyme Microb. Technol. , 28 : 625 .

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