142
Views
2
CrossRef citations to date
0
Altmetric
Original Articles

Magnetic fishing of recombinant green fluorescent proteins and tagged proteins with designed synthetic ligands

ORCID Icon, ORCID Icon, ORCID Icon & ORCID Icon
Pages 2909-2917 | Received 07 Jun 2016, Accepted 01 Sep 2017, Published online: 02 Oct 2017

References

  • Borlido, L.; Azevedo, A.; Roque, A.; Aires-Barros, M. (2013) Magnetic separations in biotechnology. Biotechnology Advances, 31 (8): 1374–1385.
  • Batalha, I.L.; Hussain, A.; Roque, A.C.A. (2010) Gum Arabic coated magnetic nanoparticles with affinity ligands specific for antibodies. Journal of Molecular Recognition, 23 (5): 462–471. doi:10.1002/jmr.1013.
  • Ditsch, A.; Yin, J.; Laibinis, P.E.; Wang, D.I.C.; Hatton, T.A. (2006) Ion-exchange purification of proteins using magnetic nanoclusters. Biotechnology Progress, 22 (4): 1153–1162. doi:10.1021/bp050290t.
  • Cerff, M.; Scholz, A.; Franzreb, M.; Batalha, I.; Roque, A.; Posten, C. (2013) In situ magnetic separation of antibody fragments from Escherichia coli in complex media. BMC Biotechnology, 13 (1): 44.
  • Santana, S.; Dhadge, V.; Roque, A. (2012) Dextran-coated magnetic supports modified with a biomimetic ligand for IgG purification. ACS Applied Materials & Interfaces, 4 (11): 5907–5914.
  • Dhadge, V.; Morgado, P.; Freitas, F.; Reis, M.; Azevedo, A.; Aires-Barros, R.; Roque, A. (2014) An extracellular polymer at the interface of magnetic bioseparations. Journal of the Royal Society Interface, 11 (100): 20140743.
  • Barroso, T.; Casimiro, T.; Ferraria, A.M.; Mattioli, F.; Aguiar-Ricardo, A.; Roque, A.C. (2014) Hybrid monoliths for magnetically-driven protein separations. Advanced Functional Materials, 24 (28): 4528–4541. doi:10.1002/adfm.201400022.
  • Dhadge, V.; Rosa, S.; Azevedo, A.; Aires-Barros, R.; Roque, A. (2014) Magnetic aqueous two phase fishing: an hybrid process technology for antibody purification. Journal of Chromatography A, 1339: 59–64.
  • Haigh, J.M.; Hussain, A.; Mimmack, M.L.; Lowe, C.R. (2009) Affinity ligands for immunoglobulins based on the multicomponent Ugi reaction. Journal of Chromatography B, 877: 1440–1452.
  • El Khoury, G.; Wang, Y.; Wang, D.; Jacob, S.; Cr, L. (2013) Design, synthesis, and assessment of a de novo affinity adsorbent for the purification of recombinant human erythropoietin. Biotechnology and Bioengineering, 110 (11): 3063–3069.
  • Pina, A.S.; Ustok, I.; El-Koury, G.; Fernandes, C.S.; Branco, R.; Lowe, C.R.; Roque, A.C.A. (2015) Mild and cost-effective green fluorescent protein purification employing small synthetic ligands. Journal of Chromatography A, 1418: 83–93.
  • Pina, A.S.; Guilherme, M.; Pereira, A.S.; Fernandes, C.S.; Branco, R.J.; El Khoury, G.; et al. (2014) A tailor-made “tag-receptor” affinity pair for the purification of fusion proteins. Chembiochem, 15 (10): 1423–1435. doi:10.1002/cbic.201400018.
  • Targovnik, A.M.; Miranda, V.; Camperi, S.A.; Cascone, O. (2012) Recombinant protein purification using complementary peptides as affinity tags. New Biotechnology, 29: 2. doi:10.1016/j.nbt.2011.05.008.
  • Fernandes, C.S.; Pina, A.S.; Dias, A.M.; Branco, R.J.; Roque, A.C.A. (2014) A theoretical and experimental approach toward the development of affinity adsorbents for GFP and GFP-fusion proteins purification. Journal of Biotechnology, 186: 13–20. doi:10.1016/j.jbiotec.2014.06.016.
  • Teng, S.F.; Sproule, K.; Hussain, A.; Lowe, C.R. (1999) A strategy for the generation of biomimetic ligands for affinity chromatography. Combinatorial synthesis and biological evaluation of an IgG binding ligand. Journal of Molecular Recognition, 12 (1): 67–75. doi:10.1002/(SICI)1099-1352(199901/02)12:1<67::AID-JMR443>3.0.CO;2-4.
  • Gray, J.J. (2006) High-resolution protein-protein docking. Current Opinion in Structural Biology, 16 (2): 183–193. doi:10.1016/j.sbi.2006.03.003.
  • Schramms, V.L.; McCluskey, R.; Emig, F.A.; Litwackg, G. (1984) Kinetic studies and active site-binding properties of glutathione S- transferase using spin-labeled glutathione, a product analogue. The Journal of Biological Chemistry, 259: 714–722.
  • Nilsson, B.; Abrahmsen, L.; Uhlén, M. (1985) Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors. The EMBO Journal, 4 (4): 1075–1080.
  • Teng, S.F.; Sproule, K.; Husain, A.; Lowe, C.R. (2000) Affinity chromatography on immobilized “‘ biomimetic ’” ligands Synthesis, immobilization and chromatographic assessment of an immunoglobulin G-binding ligand. Journal of Chromatography B, 740: 1–15.
  • Roque, A.C.A.; Taipa, M.Â.; Lowe, C.R. (2005) An artificial protein L for the purification of immunoglobulins and Fab fragments by affinity chromatography. Journal of Chromatography A, 1064 (2): 157–167. doi:10.1016/j.chroma.2004.11.102.
  • Bucak, S.; Jones, D.A.; Laibinis, P.E.; Hatton, T.A. (2003) Protein separations using colloidal magnetic nanoparticles. Biotechnology Progress, 19 (2): 477–484. doi:10.1021/bp0200853.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.