Purification of β-galactosidase from recombinant Pichia pastoris using aqueous two-phase separation technique

References

• Sen, S.; Ray, L.; Chattopadhyay, P. (2012, Aug) Production, purification, immobilization, and characterization of a thermostable β-galactosidase from aspergillusalliaceus. Applied Biochemistry and Biotechnology, 167 (7): 1938–1953.
   PubMed Web of Science ®Google Scholar
• Hemavathi, A.B.; Raghavarao, K.S.M.S. (2011, Mar) Differential partitioning of β-galactosidase and β-glucosidase using aqueous two phase extraction. Process Biochemistry, 46 (3): 649–655.
   Web of Science ®Google Scholar
• Prabhu, A.A.; Veeranki, V.D.; Dsilva, S.J. (2016, Jun) Improving the production of human interferon gamma (hIFN-γ) in Pichiapastoris cell factory: an approach of cell level. Process Biochemistry, 51 (6): 709–718.
   Web of Science ®Google Scholar
• Ansari, S.A.; Satar, R. (2012, Sep) Recombinant β-galactosidases – past, present and future: a mini review. Journal of Molecular Catalysis B: Enzymatic, 81: 1–6.
   Google Scholar
• Hung, M.-N.; Lee, B.H. (2002, Mar) Purification and characterization of a recombinant beta-galactosidase with transgalactosylation activity from Bifidobacteriuminfantis HL96. Applications of Microbiology and Biotechnology, 58 (4): 439–445.
   PubMed Web of Science ®Google Scholar
• Krishna, S.H.; Srinivas, N.D.; Raghavarao, K.S.M.S.; Karanth, N.G. (2002) Reverse micellar extraction for downstream processing of proteins/enzymes. Advances in Biochemical Engineering/Biotechnology, 75: 119–183.
   PubMedGoogle Scholar
• Chityala, A.P.A.S.; Garg, Y.; Dasu, V.V. (2017, Mar) Reverse micellar extraction of papain with cationic detergent based system: an optimization approach. Preparative Biochemistry & Biotechnology, 47 (3): 236–244.
   PubMed Web of Science ®Google Scholar
• Dhaneshwar, A.D.; Chaurasiya, R.S.; Hebbar, H.U. (2014, Jul) Process optimization for reverse micellar extraction of stem bromelain with a focus on back extraction. Biotechnology Progress, 30 (4): 845–855.
   PubMed Web of Science ®Google Scholar
• Wu, W.-C.; Ng, H.S.; Sun, I.-M.; Lan, J.C.-W. (2017, Oct) Single step purification of bromelain from Ananascomosus pulp using a polymer/salt aqueous biphasic system. Journal of the Taiwan Institute of Chemical Engineers, 79 (Supplement C): 158–162.
   Web of Science ®Google Scholar
• Pandey, R.; Prabhu, A.A.; Dasu, V.V. (2018, Feb) Purification of recombinant human interferon gamma from fermentation broth using reverse micellar extraction: a process optimization study. Separation and Purification Technology, 53 (3): 487–495.
   Google Scholar
• Silva, M.E.; Pellogia, C.; Piza, F.A.T.; Franco, T.T. (1997, Dec) Purification of three different microbial beta-galactosidases by partitioning in aqueous two-phase systems. The Journal of Food Science and Technology, 17 (3): 219–223.
   Google Scholar
• Platis, D.; Labrou, N.E. (2009, Sep) Application of a PEG/salt aqueous two-phase partition system for the recovery of monoclonal antibodies from unclarified transgenic tobacco extract. Biotechnology Journal, 4 (9): 1320–1327.
   PubMedGoogle Scholar
• O’Keefe, D.O.; DePhillips, P.; Will, M.L. (1993, Jul) Identification of an Escherichia coli protein impurity in preparations of a recombinant pharmaceutical. Pharmaceutical Research, 10 (7): 975–979.
   PubMed Web of Science ®Google Scholar
• Prabhu, A.A.; Purkayastha, A.; Mandal, B.; Kumar, J.P.; Mandal, B.B.; Dasu, V.V. (2017, Oct) A novel reverse micellar purification strategy for histidine tagged human interferon gamma (hIFN-γ) protein from Pichiapastoris. International Journal of Biological Macromolecules, 107: 2512–2514.
   Web of Science ®Google Scholar
• Glyk, A.; Scheper, T.; Beutel, S. (2015, Aug) PEG–salt aqueous two-phase systems: an attractive and versatile liquid–liquid extraction technology for the downstream processing of proteins and enzymes. Applied Microbiology and Biotechnology, 99 (16): 6599–6616.
   PubMed Web of Science ®Google Scholar
• Prabhu, A.A.; Mandal, B.; Dasu, V.V. (2017, Apr) Medium optimization for high yield production of extracellular human interferon-γ from Pichiapastoris: a statistical optimization and neural network-based approach. Korean Journal of Chemical Engineering, 34 (4): 1109–1121.
   Web of Science ®Google Scholar
• Bradford, M.M. (1976, May) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248–254.
   PubMed Web of Science ®Google Scholar
• Laemmli, U.K. (1970, Aug) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227 (5259): 680–685.
   PubMed Web of Science ®Google Scholar
• Potvin, G.; Ahmad, A.; Zhang, Z. (2012, May) Bioprocess engineering aspects of heterologous protein production in Pichiapastoris: a review. Biochemical Engineering Journal, 64: 91–105.
   Web of Science ®Google Scholar
• Ahmad, M.; Hirz, M.; Pichler, H.; Schwab, H. (2014, Apr) Protein expression in Pichiapastoris. Applied Microbiology and Biotechnology, 98 (12): 5301–5317.
   PubMed Web of Science ®Google Scholar
• Prabhu, A.A.; Boro, B.; Bharali, B.; Chakraborty, S.; Dasu, V.V. (2018, Mar) Gene and process level modulation to overcome the bottlenecks of recombinant proteins expression in Pichiapastoris. Current Pharmaceutical Biotechnology, 18: 1200–1223.
   Web of Science ®Google Scholar
• Prabhu, A.A.; Bharali, B.; Singh, A.K.; Allaka, M.; Sukumar, P.; Veeranki, V.D. (2018, May) Engineering folding mechanism through Hsp70 and Hsp40 chaperones for enhancing the production of recombinant human interferon gamma (rhIFN-γ) in Pichiapastoris cell factory. Chemical Engineering Science, 181: 58–67.
   Web of Science ®Google Scholar
• Raghavarao, K.S.M.S.; Rastogi, N.K.; Gowthaman, M.K.; Karanth, N.G. (1995, Jan) Aqueous two-phase extraction for downstream processing of enzymes/proteins. Advances in Applied Microbiology, 41: 97–171.
   Web of Science ®Google Scholar
• Raghavarao, K.S.M.S.; Ranganathan, T.V.; Srinivas, N.D.; Barhate, R.S. (2003, Jul) Aqueous two phase extraction—an environmentally benign technique. Clean Technologies and Environmental Policy, 5 (2): 136–141.
   Google Scholar
• Tou, B.S.Y.; Neo, K.E.; Tey, B.T.; Ng, M.Y.T. (2014, Jun) Effect of phase inversion and separation on hepatitis B core antigen extraction from unclarified bacterial feedstock using aqueous two-phase system. Separation and Purification Technology, 130 (Supplement C): 45–55.
   Web of Science ®Google Scholar
• Hofmeister, F. (1888) ZurLehre von der Wirkung der Salze - ZweiteMittheilung. Archiv Für Experimentelle Pathologie Und Pharmakologie, 24 (4–5): 247–260.
   Google Scholar
• Ferreira, J.F.; Santana, J.C.C.; Tambourgi, E.B. (2011, Feb) The effect of pH on bromelain partition from Ananascomosus by PEG4000/phosphate ATPS. The Brazilian Archives of Biology and Technology, 54 (1): 125–132.
   Web of Science ®Google Scholar
• Quental, M.V.; Passos, H.; Kurnia, K.A.; Coutinho, J.A.P.; Freire, M.G. (2015, Jun) Aqueous biphasic systems composed of ionic liquids and acetate-based salts: phase diagrams, densities and viscosities. The Journal of Chemical & Engineering Data, 60 (6): 1674–1682.
   PubMed Web of Science ®Google Scholar
• Najdanovic-Visak, V.; Canongia Lopes, J.N.; Visak, Z.P.; Trindade, J.; Rebelo, L.P.N. (2007, Jul) Salting-out in aqueous solutions of ionic liquids and K3PO4: aqueous biphasic systems and salt precipitation. International Journal of Molecular Sciences, 8 (8): 736–748.
   Web of Science ®Google Scholar
• Hatti-Kaul, R. (2000) Aqueous Two-Phase Systems - Methods and Protocols; Springer.
   Google Scholar
• Nagaraja, V.H.; Iyyaswami, R. (2015, Jun) Aqueous two phase partitioning of fish proteins: partitioning studies and ATPS evaluation. The Journal of Food Science and Technology, 52 (6): 3539–3548.
   PubMed Web of Science ®Google Scholar
• Madhusudhan, M.C.; Raghavarao, K.S.M.S. (2011, Oct) Aqueous two phase extraction of invertase from baker’s yeast: effect of process parameters on partitioning. Processing Biochemistry, 46 (10): 2014–2020.
   Web of Science ®Google Scholar
• Ng, H.S.; Tan, C.P.; Chen, S.K.; Mokhtar, M.N.; Ariff, A.; Ling, T.C. (2011, Oct) Primary capture of cyclodextringlycosyltransferase derived from Bacillus cereus by aqueous two phase system. Separation and Purification Technology, 81 (3): 318–324.
   Web of Science ®Google Scholar
• Amid, M.; Shuhaimi, M.; Islam Sarker, M.Z.; Abdul Manap, M.Y. (2012, Jun) Purification of serine protease from mango (MangiferaIndica Cv. Chokanan) peel using an alcohol/salt aqueous two phase system. Food Chemistry, 132 (3): 1382–1386.
   PubMed Web of Science ®Google Scholar
• Goja, A.M.; Yang, H.; Cui, M.; Li, C. (2013, Dec) Aqueous two-phase extraction advances for bioseparation. Journal of Bioprocessing & Biotechniques, 4: 1–8.
   PubMedGoogle Scholar
• Barbosa, J.M.P.; Souza, R.L.; Fricks, A.T.; Zanin, G.M.; Soares, C.M.F.; Lima, Á.S. (2011, Dec) Purification of lipase produced by a new source of Bacillus in submerged fermentation using an aqueous two-phase system. Journal of Chromatography B, 879 (32): 3853–3858.
   PubMed Web of Science ®Google Scholar
• Wongmongkol, N.; Prichanont, S. (2006, Jan) Partition of alkaline protease in aqueous two-phase systems of polyethylene glycol 1000 and potassium phosphate. Korean Journal of Chemical Engineering, 23 (1): 71–76.
   Web of Science ®Google Scholar
• Li, M.; Kim, J.-W.; Peeples, T.L. (2002, Jan) Amylase partitioning and extractive bioconversion of starch using thermoseparating aqueous two-phase systems. Journal of Biotechnology, 93 (1): 15–26.
   PubMed Web of Science ®Google Scholar
• Chen, X.; Xu, G.; Li, X.; Li, Z.; Ying, H. (2008, Jul) Purification of an α-amylase inhibitor in a polyethylene glycol/fructose-1,6-bisphosphate trisodium salt aqueous two-phase system. Processing Biochemistry, 43 (7): 765–768.
   Web of Science ®Google Scholar
• Kim, C.S.; Ji, E.-S.; Oh, D.-K. (2003, Oct) Expression and characterization of Kluyveromyceslactis beta-galactosidase in Escherichia coli. Biotechnology Letters, 25 (20): 1769–1774.
   PubMed Web of Science ®Google Scholar
• Zhou, Q.Z.K.; Chen, X.D. (2001, Nov) Effects of temperature and pH on the catalytic activity of the immobilized β-galactosidase from Kluyveromyceslactis. Biochemical Engineering Journal, 9 (1): 33–40.
   Web of Science ®Google Scholar