Hemodialysis of amino acids: Basic studies in vitro

References and notes

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• The Kiil dialyzer consists of a sandwich assembly of three polypropylene boards, with two sheets of cellophane between each set of boards that form the two blood compartments. The boards contain “V” shaped grooves, or micro‐cones, and internal porting to permit the passage of the dialyzing solution over the outer surface of each sheet of cellophane. Provisions are made to insert two plastic blood inlet and outlet ports between the cellophane sheets. The thickness of the blood passage is controlled by the differential in height between the top of the grooves and the board seating surface. The entire sandwich assembly is mechanically clamped together after the cellophane membranes are installed. The overall dimensions of the assembled dialyzer are 40 in. long, 6–5/8 in. high, and 15 1/2 in. wide. Dialyzing surface area is 1.01 m2.
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• The Drake‐Willock mobile dialysis machine has a vital conductivity meter and associated warning lights and alarms, which can be adjusted to various limits for triggering alarms if the conductivity of the dialysate deviates significantly from its normal setting. Thus, one is assured under either experimental or clinical conditions that the dialysate ionic concentrations are maintained within highly accurate predetermined limits. A photo‐electric cell monitor sounds an alarm if there are any leaks in the dialyzing membrane that permit red cells to pass through. When whole blood was not employed in these experiments, a sample of blood or of packed red cells was always added at the conclusion of the experiment to test for holes in the dialyzing membrane. A blood pressure manometer, which registers the blood pressure in the drip bulb, is part of the equipment of this instrument. If the line blood pressure should drop past the preset lower limit or if the blood pressure rises to exceed the high limit setting, the alarm will sound in the console, providing an accurate means to control and monitor the pressure of the “blood” and the influence of our blood pump. Pressure in the dialysate compartment was controlled by a negative pressure gauge calibrated in inches of water (one in. of water =1.9 mm of mercury).
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• The dialysate solution was manufactured by McGaw and marketed under the trade name of “Hemotrate‐K” Catalog No. R‐1686, Formula 9.
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• Address correspondence to: Dr. Vernon Riley, Department of Microbiology, Pacific Northwest Research Foundation, 1102/1124 Columbia Street, Seattle, Washington 98104.
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