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Original Articles

Creatine supplementation does not decrease oxidative stress and inflammation in skeletal muscle after eccentric exercise

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Pages 1164-1176 | Accepted 01 Feb 2013, Published online: 05 Apr 2013

References

  • Adcock , K. H. , Nedelcu , J. , Loenneker , T. , Martin , E. , Wallimann , T. and Wagner , B. P. 2002 . Neuroprotection of creatine supplementation in neonatal ruts with transient cerebral Kypoxia-iachemia . Developmental Neuroscience , 24 : 382 – 388 .
  • Aebi , H. 1984 . Catalase in vitro . Methods in Enzymology , 105 : 121 – 126 .
  • Aksenov , M. Y. and Markesbery , W. R. 2001 . Changes in thiol content and expression of glutathione redox system genes in the hippocampus and cerebellum in Alzheimer's disease . Neuroscience Letters , 302 : 141 – 145 .
  • Alessio , H. M. 1993 . Exercise-induced oxidative stress . Medicine and Science in Sports and Exercise , 25 : 218 – 224 .
  • Allen , M. and Tresini , R. G. 2000 . Oxidative stress and gene regulation . Free Radical Biology and Medicine , 28 : 463 – 499 .
  • Alves, C. R., Murai, I. H., Ramona, P., Nicastro, H., Bechara, L.R., Lancha, A. H Jr, Brum P. C., Irigoyen M. C., Gualano, B. (2012). No effect of creatine supplementation on oxidative stress and cardiovascular parameters in spontaneously hypertensive rats. J Int Soc Sports Nutr, 9, 13. ,
  • Arciero , P. J. , Hannibal , N. S. , Nindl , B. C. , Gentile , C. L. , Hamed , J. and Vukovich , M. D. 2001 . Comparison of creatine ingestion and resistance training on energy expenditure and limb blood flow . Metabolism , 50 : 1429 – 1434 .
  • Armstrong , R. B. , Ogilvie , R. W. and Schwane , J. A. 1983 . Eccentric exercise-induced injury to rat skeletal muscle . Journal of Applied Physiology , 54 : 80 – 93 .
  • Balsom , P. D. , Soderlund , K. and Ekblom , B. 1994 . Creatine in humans with special reference to creatine supplementation . Sports Medicine , 18 : 268 – 280 .
  • Balsom , P. D. , Soderlund , K. , Sjodin , B. and Ekblom , B. 1995 . Skeletal muscle metabolism during short duration high intensity exercise: Influence of creatine supplementation . Acta Physiologica Scandinavica , 154 : 303 – 310 .
  • Bassit , R. A. , Pinheiro , C. H. , Vitzel , K. F. , Sproesser , A. J. , Silveira , L. R. and Curi , R. 2010 . Effect of short-term creatine supplementation on markers of skeletal muscle damage after strenuous contractile activity . European Journal of Applied Physiology , 108 : 945 – 955 .
  • Beaton , L. J. , Tarnopolsky , M. A and Phillips , S. M. 2002 . Contraction-induced muscle damage in humans following calcium channel blocker administration . J Physiol , 544 : 849 – 859 .
  • Benzi , G. 2000 . Is there a rationale for the use of creatine either as nutritional supplementation or drug administration in humans participating in a sport? . Pharmacology Research , 41 : 255 – 264 .
  • Bradford , M. M. 1976 . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding . Analytical Biochemistry , 72 : 248 – 254 .
  • Brooks , G. A. and White , T. P. 1978 . Determination of metabolic and heart rate responses of rats to treadmill exercise . Journal of Applied Physiology , 45 : 1009 – 1015 .
  • Calabrese , E. J. and Baldwin , L. A. 2003 . Hormesis: the dose-response revolution . Annu Rev Pharmacol Toxicol , 43 : 175 – 197 .
  • Cannon, J. G., Orencole, S. F., Fielding, R. A., Meydani, M., Meydani, S. N., Fiatarone, M. A. et al. (1990). Acute phase response in exercise: Interaction of age and vitamin E on neutrophils and muscle enzyme release. American Journal of Physiology, 259, 1214–1219.
  • Casey , A. , Constantin-Teodosiu , D. , Howell , S. , Hultman , E. and Greenhaff , P. L. 1996 . Creatine ingestion favorably affects performance and muscle metabolism during maximal exercise in humans . American Journal of Physiology , 271 : E31 – E37 .
  • Chiang, J., Shen, Y. C., Wang, Y. H., Hou, Y. C., Chen, C. C., Liao, J. F. et al. (2009). Honokiol protects rats against eccentric exercise-induced skeletal muscle damage by inhibiting NFkappaB induced oxidative stress and inflammation. European Journal of Pharmacology, 610, 119–127.
  • Clarkson , P. M. , Nosaka , K. and Braun , B. 1992 . Muscle function after exercise-induced muscle damage and rapid adaptation . Medicine and Science in Sports and Exercise , 4 : 512 – 520 .
  • Cooney , R. N. , Maish , G. O. , Gilpin , T. , Shumate , M. L. , Lang , C. H. and Vary , T. C. 1999 . Mechanism of IL-1 induced inhibition of protein synthesis in skeletal muscle . Shock , 11 : 235 – 241 .
  • Dalton , T. P. , Shertzer , H. G. and Puga , A. 1999 . Regulation of gene expression by reactive oxygen . Annual Review of Pharmacology and Toxicology , 39 : 67 – 101 .
  • Demant , T. W. and Rhodes , E. C. 1999 . Effects of creatine supplementation on exercise performance . Sports Medicine , 28 : 49 – 60 .
  • Dentowski , A. B. H. , Opaszowski , D. , Blachnio , D. and Polanowski , B. 1997 . Effect of creatine supplementation on the performance in supramaximal, intermittent exercise . Biology of Sport , 14 : 291 – 298 .
  • Di Meo , S. and Venditti , P. 2001 . Mitochondria in exercise-induced oxidative stress . Biological Signals and Receptors , 10 : 125 – 140 .
  • Draper , H. H. and Hadley , M. 1990 . Malondialdehyde determination as index of lipid peroxidation . Methods in Enzymology , 186 : 421 – 431 .
  • Droge , W. 2002 . Free radicals in the physiological control of cell function . Physiol Rev , 82 : 47 – 95 .
  • Ferreira , A. P. P. , Salvador , R. , Elizabeth , F. K. , de Sousa , C. K. and Fernandes , F. R. H . 2012 . Influência da suplementação de creatina na capacidade funcional de pacientes com Insuficiência Cardíaca . Arq Bras Cardio , 99 : 623 – 629 .
  • Fielding , R. A. , Manfredi , T. J. , Ding , W. , Fiatarone , M. A. , Evans , W. J. and Cannon , J. G. 1993 . Acute phase response in exercise: III. Neutrophil and IL-1 beta accumulation in skeletal muscle . American Journal of Physiology , 265 : 166 – 172 .
  • Finaud , J. , Lac , G. and Filaire , E. 2006 . Oxidative stress: Relationship with exercise and training . Sports and Medicine , 36 : 327 – 358 .
  • Flohé , L. and Gunzler , W. 1984 . Assays of glutathione peroxidase . Methods in Enzymology , 105 : 114 – 121 .
  • Goldspink , G . 1994 . “ Cellular and molecular aspects of adaptation in skeletal muscle ” . In In Strength and power in sport , pp. 211 – 229) . Oxford, UK : Blackwell Science .
  • Gomez-Cabrera , M. C. , Domenech , E. , Ji , L. L. and Viña , J . 2006 . Exercise as an antioxidant: it up-regulates important enzymes for cell adaptations to exercise . Science & Sports , 2 : 185 – 189. .
  • Gomez-Cabrera , M. C. , Domenech , E. and Viña , J . 2008 . Moderate exercise is an antioxidant: upregulation of antioxidant genes by training . Free Radic Biol Med , 44 : 126 – 131 .
  • Graham , A. S. and Hatton , R. C. 1999 . Creatine: A review of efficacy and safety . Journal of the American Pharmacists Association , 39 : 803 – 810 .
  • Greenhaff, P. L. (1997). The nutritional biochemistry of creatine. J Nutr Biochem, 8, 610–618. ,
  • Halliwell , B. and Gutteridge , J. M. C. 2007 . Free Radicals in Biology and Medicine , New York, NY : Oxford University Press .
  • Hamada , K. , Vannier , E. , Sacheck , J. M. , Witsell , A. L. and Roubenoff , R. 2004 . Senescence of human skeletal muscle impairs the local inflammatory cytokine response to acute eccentric exercise . FASEB Journal , 9 : 264 – 266 .
  • Harris , R. C. , Soderlund , K. and Hultman , E. 1992 . Elevation of creatine in resting and exercised muscle of normal subjects by creatine supplementation . Clinical Science , 83 : 367 – 374 .
  • Holtzman, D., Khait, I., Mulkern, R., Allred, E., Rand, T., Jensen, F., et al. (1999). In vivo development of brain phosphocreatine in normal and creatine-treated rabbit pups. Journal of Neurochemistry, 73, 2477–2484.
  • Jackson , M. J. 2000 . “ Exercise and oxygen radical production by muscle ” . In Handbook of oxidants and antioxidants in exercise , Edited by: Sen , C. K. , Packer , L. and Hanninen , O. pp. 57 – 68 . Amsterdam : Elsevier Science . (Eds.),
  • Jackson , M. J . 2005 . Reactive oxygen species and redox regulation of skeletal muscle adaptations to exercise . Philos Trans R Soc Lond B Biol Sci , 360 : 2285 – 2291 . .,
  • Jaeschke , H. 1995 . Mechanisms of oxidant stress-induced acute tissue injury . Proceedings of the Society for Experimental Biology and Medicine , 209 : 104 – 111 .
  • Juhn , M. S. and Tarnopolsky , M. 1998 . Oral creatine supplementation and athletic performance: A critical review . Clinical Journal of Sport and Medicine , 8 : 286 – 297 .
  • Kasparova, S., Brezova, V., Valko, M., Horecky, J., Mlynarik, V., Liptaj, T., et al. (2005). Study of the oxidative stress in a rat model of chronic brain hypoperfusion. Neurochemistry International, 46, 601–611.
  • Kasparova, S., Dobrota, D., Mlynarik, V., Pham, T. N., Liptaj, T., Horecky, J., et al. (2000). A study of creatine kinase reaction in rat brain under chronic pathological conditions — chronic ischemia and ethanol intoxication. Brain Research Bulletin, 53, 431–435.
  • Kerksick , C. M. , Kreider , R. B. and Willoughby , D. S. 2010 . Intramuscular adaptations to eccentric exercise and antioxidant supplementation . Amino Acids , 39 : 219 – 232 .
  • Khassaf, M., McArdle, A., Esanu, C., Vasilaki, A., McArdle, F., Griffiths, R. D., et al. (2003). Effect of vitamin C supplements on antioxidant defense and stress proteins in human lymphocytes and skeletal muscle. Journal of Physiology, 549, 645–652.
  • Kilduff , L. P. , Vidakovic , P. , Cooney , G. , Twycross-Lewis , R. , Amuna , P. , Parker , M. and Paul , L. 2002 . Effects of creatine on isometric bench-press performance in resistance-trained humans . Medicine and Science in Sports and Exercise , 34 : 1176 – 1183 .
  • Kraemer , W. J. and Volek , J. S. 1999 . Creatine supplementation. Its role in human performance . Clinical Sports Medicine , 18 : 651 – 666 .
  • Laemmli , U. K. 1970 . Cleavage of structural proteins during theassembly of the head of bacteriophage T4 . Nature , 227 : 680 – 685 .
  • Lambertucci , R. H. , Levada-Pires , A. C. , Rossoni , L. V. , Curi , R. and Pithon-Curi , T. C. 2007 . Effects of aerobic exercise training on antioxidant enzyme activities and mRNA levels in soleus muscle from young and aged rats . Mechanisms of Ageing and Development , 128 : 267 – 275 .
  • Lawler , J. M. , Barnes , W. S. , Wu , G. , Song , W. and Demaree , S. 2002 . Direct antioxidant properties of creatine . Biochemical and Biophysical Research Communication , 290 : 47 – 52 .
  • Levine , R. L. 2002 . Carbonyl modified proteins in cellular regulation, aging and disease . Free Radical Biology and Medicine , 32 : 790 – 796 .
  • Levine, R. L., Garland, D., Oliver, C. N., Amici, A., Climent, I., Lenz, A. G., et al. (1990). Determination of carbonyl content in oxidatively modified proteins. Methods in Enzymology, 186, 464–478.
  • Liao , P. , Zhou , J. , Ji , L. L. and Zhang , Y. 2010 . Eccentric contraction induces inflammatory responses in rat skeletal muscle: Role of tumor necrosis factor-alpha . American Journal of Physiology - Regulatory Integrative and Comparative Physiology , 2983 : 599 – 607 .
  • Lowry , O. H. , Rosebrough , N. J. , Farr , A. L. and Randall , R. J. 1951 . Protein measurement with the Folin phenol reagent . Journal of Biological Chemistry , 193 : 265 – 267 .
  • Matthews, R. T., Ferrante, R. J., Klivenyi, P., Yang, L., Klein, A. M., Mueller, G., Kaddurah-Daouk, R., et al. (1999). Creatine and cyclocreatine attenuate MPTP neurotoxicity. Experimental Neurology, 157, 142–149.
  • McCord, J. M., & Fridovich, I. (1969). Superoxide dismutase: an enzymic function for erythrocuprein (hemocuprein). J. Biol. Chem, 244, 6049–6055. ,
  • Molnar, A. M., Servais, S., Guichardant, M., Lagarde, M., Macedo, D. V., Pereira-Da-Silva, L., et al., (2006). Mitochondrial H2O2 production is reduced with acute and chronic eccentric exercise in rat skeletal muscle. Antioxidant and Redox Signaling, 8, 548–558.
  • Moopanar , T. R. and Allen , D. G. 2005 . Reactive oxygen species reduce myofibrillar Ca2 + ‑sensitivity in fatiguing mouse skeletal muscle at 37°C . Journal of Physiology , 564 : 189 – 199 .
  • Mujika , I. and Padilla , S. 1997 . Creatine supplementation as an ergogenic aid for sports performance in highly trained athletes: A critical review . International Journal of Sports Medicine , 18 : 491 – 496 .
  • Nader , G. A. and Esser , K. A . 2001 . Intracellular signaling specificity in skeletal muscle in response to different modes of exercise . J Appl Physiol , 90 : 1936 – 1942 . .,
  • Oh-Ishi , S. , Kizaki , T. , Yamashita , H. , Nagata , N. , Suzuki , K. , Taniguchi , N. and Ohno , H . 1995 . Alterations of superoxide dismutase iso-enzyme activity, content, and mRNA expression with aging in rat skeletal muscle . Mech Ageing Dev , 84 : 65 – 76 .
  • Olert , E. D. , Cross , B. M. and McWilliam , A. A. 1993 . Guide to care and use of experimental animals , Ottawa : Canadian Council on Animal .
  • Peake , J. , Nosaka , K. and Suzuki , K. 2005 . Characterization of inflammatory responses to eccentric exercise in humans . Exercise Immunology Review , 11 : 64 – 85 .
  • Pearlman , J. P. and Fielding , R. A. 2006 . Creatine monohydrate as a therapeutic aid in muscular dystrophy . Nutrition Reviews , 64 : 80 – 88 .
  • Persky, A. M., & Brazeau, G. A., Clinical pharmacology of the dietary supplement creatine monohydrate. Pharmacological Reviews, 53, 161–176.
  • Person , P. B. and Henriksson , J. 2011 . Good publication practice in physiology . Acta Physiologica , 203 : 403 – 407 .
  • Poderoso , J. J. , Carreras , M. C. and Lisdero , C. L. 1996 . Nitric oxide inhibits electron transfer and increases superoxide radical production in rat heart mitochondrial and submitochondrial particles . Archives of Biochemistry and Biophysics , 328 : 85 – 92 .
  • Prass, K., Royl, G., Lindauer, U., Freyer, D., Megow, D., Dirnagl, U., et al., (2006). Improved reperfusion and neuroprotection by creatine in a mouse model of stroke. Journal of Cerebral Blood Flow and Metabolism, 27, 452–459.
  • Radak , Z , Chung , H. Y. and Goto , S . 2005 . Exercise and hormesis: Oxidative stressrelated adaptation for successful aging. An Hypothesis . Biogerontology , 61 : 71 – 75 . ,
  • Rakpongsiri, K., & Sawangkoon, S. (2008). Protective effect of creatine supplementation and estrogen replacement on cardiac reserve function and antioxidant reservation against oxidative stress in exercise-trained ovariectomized hamsters. Int Heart J, 49, 343–54. ,
  • Rawson , E. S. , Conti , M. P. and Miles , M. P. 2007 . Creatine supplementation does not reduce muscle damage or enhance recovery from resistance exercise . Journal of Strength and Conditioning Research , 21 : 1208 – 1213 .
  • Rawson , E. S. , Gunn , B. and Clarkson , P. M. 2001 . The effects of creatine supplementation on exercise-induced muscle damage . Journal of Strength and Conditioning Research , 15 : 178 – 184 .
  • Rawson , E. S. and Volek , J. S. 2003 . Effects of creatine supplementation and resistance training on muscle strength and weightlifting performance . Journal of Strength and Conditioning Research , 4 : 822 – 831 .
  • Rodriguez, C., Mayo, J. C., Sainz, R. M., Antolin, I., Herrera, F., Martin, V., et al., (2004). Regulation of antioxidant enzymes: Asignificant role for melatonin. Journal of Pineal Research, 36, 1–9.
  • Roschel , H. , Gualano , B. , Marquezi , M. , Costa , A. and Lancha , A. H. 2010 . Creatine supplementation spares muscle glycogen during high intensity intermittent exercise in rats . Journal of the International Society of Sports Nutrition , 7 : 1 – 7 .
  • Sakamoto , A. , Maruyama , T. , Naito , H. and Sinclair , P. J. 2010 . Acute effects of high-intensity dumbbell exercise after isokinetic eccentric damage: Interaction between altered pain perception and fatigue on static and dynamic muscle performance . Journal of Strength and Conditioning Research , 8 : 2042 – 2049 .
  • Salminen , A. and Vihko , V . 1983 . Endurance training reduces the susceptibility of mouse skeletal muscle to lipid peroxidation in vitro . Acta Physiol Scand , 117 : 109 – 113 .
  • Santos , R. V. T , Bassit , R. A. , Caperuto , E. C. and Costa Rosa , L. F. B. 2004 . The effect of creatine supplementation upon inflammatory and muscle soreness markers after a 30 km race . Life Science , 75 : 1917 – 1924 .
  • Sestili, P., Martinelli, C., Bravi, G., Piccoli, G., Curci, R., Battistelli, M., et al., (2006). Creatine supplementation affords cytoprotection in oxidatively injured cultured mammalian cells via direct antioxidant activity. Free Radical Biology and Medicine, 40, 837–849.
  • Sestili, P., Martinelli, C., Colombo, E., Barbieri, E., Potenza, L., Sartini, S., & Fimognari, C. (2011). Creatine as an antioxidant. Amino Acids, 40, 1385–1396. ,
  • Silva , L. A. , Pinho , C. A. , Silveira , P. C. , Tuon , T. , De Souza , C. T. , Dal-Pizzol , F. and Pinho , R. A. 2010 . Vitamin E supplementation decreases muscular and oxidative damage but not inflammatory response induced by eccentric contraction . Journal of Physiology Science , 60 : 51 – 57 .
  • Silva , L. A. , Silveira , P. C. L. , Pinho , C. A. , Tuon , T. , Dal-Pizzol , F. and Pinho , R. A. 2008 . N-acetylcysteine supplementation and oxidative damage and inflammatory response after eccentric exercise . International Journal of Sports Nutrition and Exercise Metabolism , 18 : 393 – 402 .
  • Silva, L. A., Silveira, P. C. L., Rosani, M. M., Souza, P. S., Vieira, C. L., Souza, C. T., Pinho, R. A. (2011). Taurine supplementation decreases oxidative stress in skeletal muscle after eccentric exercise. Cell Biochemistry and Function, 29, 43–49.
  • Sharov, V. G., Saks, V. A., Kupriyanov, V. V., Lakomkin, V. L., Kapelko, V. I., Steinschneider, A., et al., (1987). Protection of ischemic myocardium by exogenous phosphocreatine. I. Morphologic and phosphorus 31-nuclear magnetic resonance studies. The Journal of Thoracic and Cardiovascular Surgery, 94, 749–761.
  • Sjodin , B. , Hellsten , W. Y. and Apple , F. S. 1990 . Biochemical mechanisms for oxygen free radical formation during exercise . Sports Medicine , 10 : 236 – 254 .
  • Stupka , N. , Tarnopolsky , M. A. , Yardley , N. J. and Phillips , S. M. 2001 . Cellular adaptation to repeated eccentric exercise-induced muscle damage . Journal of Applied Physiology , 91 : 1669 – 1678 .
  • Szweda , P. A. , Friguet , B. and Szweda , L. I. 2002 . Proteolysis, free radicals and aging . Free Radical Biology and Medicine , 33 : 29 – 36 .
  • Tarnopolsky, M. A., Bourgeois, J. M., Snow, R., Keys, S., Roy, B.D., Kwiecien, J. M., & Turnbull, J. (2003). Histological assessment of intermediate- and long-term creatine monohydrate supplementation in mice and rats. Am J Physiol Regul Integr Comp Physiol, 285, R762–R769. ,
  • Tauler , P. , Ferrer , M. D. , Romaguera , D. , Sureda , A. , Aguilo , A. , Tur , J. and Pons , A. 2008 . Antioxidant response and oxidative damage induced by a swimming session: Influence of gender . Journal of Sports Sciences , 26 : 1303 – 1311 .
  • Theodorou, A. A., Nikolaidis, M. G., Paschalis, V., Koutsias, S., Panayiotou, G., Fatouros, I. G., et al., (2011). No effect of antioxidant supplementation on muscle performance and blood redox status adaptations to eccentric training. American Journal of Clinical Nutrition, 93, 1373–1383.
  • Trent , F. R. and Allen , D. G. 2009 . Iron injections in mice increase skeletal muscle iron content, induce oxidative stress and reduce exercise performance . Experimental Physiology , 94 : 720 – 730 .
  • Van Der Poel , C. and Stephenson , D. G. 2002 . Reversible changes in Ca2 + ‑activation properties of rat skeletal muscle exposed to elevated physiological temperatures . Journal of Physiology , 544 : 765 – 776 .
  • Vina , J. , Borras , C. , Gomez-Cabrera , M. C. and Orr , W. C . 2006 . Importance of reactive oxygen species to modulate molecular and cellular response to stress . Free Rad Res , 402 : 111 – 119 .
  • Volek , W. K. 1997 . Cr supplementation: Its effect on human muscular performance and body composition . Journal of Strength and Conditioning Research , 10 : 200 – 210 .
  • Walker , J. B. 1979 . Creatine: Biosynthesis, regulation, and function . Advances in Enzymology and Related Areas of Molecular Biology , 50 : 177 – 242 .
  • Wallimann, T., Tokarska-Schlattner, M., & Schlattner, U. (2011). The creatine kinase system and pleiotropic effects of creatine. Amino Acids, 40, 1271–1296. ,
  • Wei , Y. H. and Lee , H. C. 2002 . Oxidative stress, mitochondrial DNA mutation, and impairment of antioxidant enzymes in aging . Experimental Biology and Medicine , 227 : 671 – 682 .
  • Wilken , B. , Ramihk , J. M. , Prohst , I. , Richter , D. W. and Hanekeld , E. 2000 . Anoxic ATP depletion in neonatal mice brainstem is prevented by creatine supplementation . Archives of Disease in Childhood - Fetal Neonatal , 82 : 224 – 227 .
  • Wyss, M., & Kaddurah-Daouk, R. (2000). Creatine and creatinine metabolism. Physiol Rev, 80, 1107–1213. ,
  • Wyss , M. and Schulze , A. 2002 . Health implications of creatine: Can oral creatine supplementation protect against neurological and atherosclerotic disease? . Neuroscience , 112 : 243 – 260 .
  • Zhou , L. Z. , Johnson , A. P. and Rando , T. A. 2001 . NF kappa B and AP-1 mediate transcriptional responses to oxidative stress in skeletal muscle cells . Free Radical Biology and Medicine , 31 : 1405 – 1416 .

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