Non-viral gene therapy for bone tissue engineering

References

• Aggarwal , R. , Pompili , V. J. and Das , H. 2010 . Genetic modification of ex-vivo expanded stem cells forclinical application . Frontiers in Bioscience , 15 : 854 – 871 .
   Google Scholar
• Aggarwal , S. and Pittenger , M. F. 2005 . Human mesenchymal stem cells modulate allogeneic immune cell responses . Blood , 105 : 1815 – 1822 .
   PubMed Web of Science ®Google Scholar
• Ahn , H. H. , Lee , J. H. , Kim , K. S. , Lee , J. Y. , Kim , M. S. , Khang , G. , … and Lee , H. B. 2008 . Polyethyleneimine-mediated gene delivery into human adipose derived stem cells . Biomaterials , 29 : 2415 – 2422 .
   PubMed Web of Science ®Google Scholar
• Arthur , A. , Zannettino , A. and Gronthos , S. 2009 . The therapeutic applications of multipotential mesenchymal/stromal stem cells in skeletal tissue repair . Journal of Cell Physiology , 218 : 237 – 245 .
   PubMed Web of Science ®Google Scholar
• Blum , J. S. , Barry , M. A. , Mikos , A. G. and Jansen , J. A. 2003 . In vivo evaluation of gene therapy vectors in ex vivo-derived marrow stromal cells for bone regeneration in a rat critical-size calvarial defect model . Human Gene Therapy , 14 : 1689 – 1701 .
   PubMed Web of Science ®Google Scholar
• Bonadio , J. , Smiley , E. , Patil , P. and Goldstein , S. 1999 . Localized, direct plasmid gene delivery in vivo: prolonged therapy results in reproducible tissue regeneration . Nature Medicine , 5 : 753 – 759 .
   PubMed Web of Science ®Google Scholar
• Bose , S. and Tarafder , S. 2012 . Calcium phosphate ceramic systems in growth factor and drug delivery for bone tissue engineering: a review . Acta Biomaterialia , 8 : 1401 – 1421 .
   PubMed Web of Science ®Google Scholar
• Bourgeat-Lami , E. 2002 . Organic-inorganic nanostructured colloids . Journal of Nanoscience and Nanotechnology , 2 : 1 – 24 .
   PubMed Web of Science ®Google Scholar
• Carragee , E. J. , Hurwitz , E. L. and Weiner , B. K. 2011 . A critical review of recombinant human bone morphogenetic protein-2 trials in spinal surgery: emerging safety concerns and lessons learned . Spine Journal , 11 : 471 – 491 .
   PubMed Web of Science ®Google Scholar
• Chew , S. A. , Kretlow , J. D. , Spicer , P. P. , Edwards , A. W. , Baggett , L. S. , Tabata , Y. , … and Mikos , A. G. 2011 . Delivery of plasmid DNA encoding bone morphogenetic protein-2 with a biodegradable branched polycationic polymer in a critical-size rat cranial defect model . Tissue Engineering Part A , 17 : 751 – 763 .
   PubMed Web of Science ®Google Scholar
• Chimutengwende-Gordon , M. and Khan , W. S. 2012 . Advances in the use of stem cells and tissue engineering applications in bone repair . Current Stem Cell Research and Therapy , 7 : 122 – 126 .
   PubMedGoogle Scholar
• Choi , Y. H. , Liu , F. , Choi , J. S. , Kim , S. W. and Park , J. S. 1998 . Polyethylene glycol-grafted poly-L lysine as polymeric gene carrier . Journal of Controlled Release , 54 : 39 – 48 .
   PubMed Web of Science ®Google Scholar
• Chowdhury , E. H. and Akaike , T. 2005 . Bio-functional inorganic materials: An attractive branch of gene-based nano-medicine delivery for 21st century . Current Gene Therapy , 5 : 669 – 676 .
   PubMed Web of Science ®Google Scholar
• Clements , B. A. , Incani , V. , Kucharski , C. , Lavasanifar , A. , Ritchie , B. and Uludağ , H. 2007 . A comparative evaluation of poly-L-lysine-palmitic acid and Lipofectamine 2000 for plasmid delivery to bone marrow stromal cells . Biomaterials , 28 : 4693 – 4704 .
   PubMed Web of Science ®Google Scholar
• Clinical trial database. Retrieved from http://www.wiley.com/legacy/wileychi/genmed/clinical/
   Google Scholar
• Corsi , K. , Chellat , F. , Yahia , L. and Fernandes , J. C. 2003 . Mesenchymal stem cells, MG63 and HEK293 transfection using chitosan-DNA nanoparticles . Biomaterials , 24 : 1255 – 1264 .
   PubMed Web of Science ®Google Scholar
• Dang , J. M. and Leong , K. W. 2006 . Natural polymers for gene delivery and tissue engineering . Advanced Drug Delivery Reviews , 58 : 487 – 499 .
   PubMed Web of Science ®Google Scholar
• Deng , M. , James , R. , Laurencin , C. T. and Kumbar , S. G. 2012 . Nanostructured polymeric scaffolds for orthopaedic regenerative engineering . IEEE Transactions in Nanobioscience , 11 : 3 – 14 .
   PubMedGoogle Scholar
• Düzgüneş , N. , De Ilarduya , C. T. , Simões , S. , Zhdanov , R. I. , Konopka , K. and Pedroso de Lima , M. C. 2003 . Cationic liposomes for gene delivery: Novel cationic lipids and enhancement by proteins and peptides . Current Medicinal Chemistry , 10 : 1213 – 1220 .
   PubMed Web of Science ®Google Scholar
• Fang , J. , Zhu , Y. Y. , Smiley , E. , Bonadio , J. , Rouleau , J. P. , Goldstein , S. A. , … and Roessler , B. J. 1996 . Stimulation of new bone formation by direct transfer of osteogenic plasmid genes . Proceedings of the National Academy of Science of the United States of America , 93 : 5753 – 5758 .
   PubMed Web of Science ®Google Scholar
• Ferreira , E. , Potier , E. , Logeart-Avramoglou , D. , Salomskaite-Davalgiene , S. , Mir , L. M. and Petite , H. 2008 . Optimization of a gene electrotransfer method for mesenchymal stem cell transfection . Gene Therapy , 15 : 537 – 544 .
   PubMed Web of Science ®Google Scholar
• Fischer , D. , Bieber , T. , Li , Y. , Elsässer , H. P. and Kissel , T. 1999 . A novel non-viral vector for DNA delivery based on low molecular weight, branched polyethylenimine: effect of molecular weight on transfection efficiency and cytotoxicity . Pharmaceutical Research , 16 : 1273 – 1279 .
   PubMed Web of Science ®Google Scholar
• Franceschi , R. T. , Wang , D. , Krebsbach , P. H. and Rutherford , R. B. 2000 . Gene therapy for bone formation: In vitro and in vivo osteogenic activity of an adenovirus expressing BMP7 . Journal of Cell Biochemistry , 78 : 476 – 486 .
   PubMed Web of Science ®Google Scholar
• Garcia-Fuentes , M. and Alonso , M. J. 2012 . Chitosan-based drug nanocarriers: Where do we stand? . Journal of Controlled Release , 161 : 496 – 504 .
   PubMed Web of Science ®Google Scholar
• Gill , D. R. , Pringle , I. A. and Hyde , S. C. 2009 . Progress and prospects: The design and production of plasmid vectors . Gene Therapy , 16 : 165 – 171 .
   PubMed Web of Science ®Google Scholar
• Glassman , S. D. , Gum , J. L. , Crawford , C. H. 3rd , Shields , C. B. and Carreon , L. Y. 2011 . Complications with recombinant human bone morphogenetic protein-2 in posterolateral spine fusion associated with a dural tear . Spine Journal , 11 : 522 – 526 .
   PubMedGoogle Scholar
• Godbey , W. T. , Barry , M. A. , Saggau , P. , Wu , K. K. and Mikos , A. G. 2000 . Poly(ethylenimine)-mediated transfection: A new paradigm for gene delivery . Journal of Biomedical Materials Research , 51 : 321 – 328 .
   PubMed Web of Science ®Google Scholar
• Gwak , S. J. and Kim , B. S. 2008 . Poly(lactic-co-glycolic acid) nanosphere as a vehicle for gene delivery to human cord blood-derived mesenchymal stem cells: comparison with polyethylenimine . Biotechnology Letters , 30 : 1177 – 1182 .
   PubMedGoogle Scholar
• Han , S. W. , Nakamura , C. , Kotobuki , N. , Obataya , I. , Ohgushi , H. , Nagamune , T. and Miyake , J. 2008 . High-efficiency DNA injection into a single human mesenchymal stem cell using a nanoneedle and atomic force microscopy . Nanomedicine , 4 : 215 – 225 .
   PubMed Web of Science ®Google Scholar
• He , X. , Dziak , R. , Mao , K. , Genco , R. , Swithart , M. , Li , C. and Yang , S. 2013 . Integration of a novel injectable nano calcium sulfate/alginate scaffold and BMP2-gene modified MSCs for bone regeneration . Tissue Engineering Part A , 19 : 508 – 518 .
   PubMed Web of Science ®Google Scholar
• Hedley , M. L. , Curley , J. and Urban , R. 1998 . Microspheres containing plasmid-encoded antigens elicit cytotoxic T-cell responses . Nature Medicine , 4 : 365 – 368 .
   PubMed Web of Science ®Google Scholar
• Hoekstra , D. , Rejman , J. , Wasungu , L. , Shi , F. and Zuhorn , I. 2007 . Gene delivery by cationic lipids: In and out of an endosome . Biochemical Society Transactions , 35 : 68 – 71 .
   PubMed Web of Science ®Google Scholar
• Humbert , J. M. and Halary , F. 2012 . Viral and non-viral methods to genetically modify dendritic cells . Current Gene Therapy , 12 : 127 – 136 .
   PubMedGoogle Scholar
• Hyde , S. C. , Pringle , I. A. , Abdullah , S. , Lawton , A. E. , Davies , L. A. , Varathalingam , A. , … and Gill , D. R. 2008 . CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression . Nature Biotechnology , 26 : 549 – 551 .
   PubMed Web of Science ®Google Scholar
• Incani , V. , Tunis , E. , Clements , B. A. , Olson , C. , Kucharski , C. , Lavasanifar , A. and Uludag , H. 2007 . Palmitic acid substitution on cationic polymers for effective delivery of plasmid DNA to bone marrow stromal cells . Journal of Biomedical Material Research A , 81 : 493 – 504 .
   PubMedGoogle Scholar
• Kasper , F. K. , Kushibiki , T. , Kimura , Y. , Mikos , A. G. and Tabata , Y. 2005 . In vivo release of plasmid DNA from composites of oligo(poly(ethylene glycol)fumarate) and cationized gelatin microspheres . Journal of Controlled Release , 107 : 547 – 561 .
   PubMed Web of Science ®Google Scholar
• Kasper , F. K. , Young , S. , Tanahashi , K. , Barry , M. A. , Tabata , Y. , Jansen , J. A. and Mikos , A. G. 2006 . Evaluation of bone regeneration by DNA release from composites of oligo(poly(ethylene glycol) fumarate) and cationized gelatin microspheres in a critical-sized calvarial defect . Journal of Biomedical Material Research A , 78 : 335 – 342 .
   PubMedGoogle Scholar
• Kawai , M. , Maruyama , H. , Bessho , K. , Yamamoto , H. , Miyazaki , J. and Yamamoto , T. 2009 . Simple strategy for bone regeneration with a BMP-2/7 gene expression cassette vector . Biochemical Biophysiology Research Community , 390 : 1012 – 1017 .
   PubMedGoogle Scholar
• Kay , M. A. 2011 . State-of-the-art gene-based therapies: the road ahead . Nature Review Genetics , 12 : 316 – 328 .
   PubMed Web of Science ®Google Scholar
• Kempen , D. H. , Creemers , L. B. , Alblas , J. , Lu , L. , Verbout , A. J. , Yaszemski , M. J. and Dhert , W. J. 2010 . Growth factor interactions in bone regeneration . Tissue Engineering Part B Reviews , 16 : 551 – 566 .
   PubMedGoogle Scholar
• Klyushnenkova , E. , Mosca , J. D. , Zernetkina , V. , Majumdar , M. K. , Beggs , K. J. , Simonetti , D. W. , … and McIntosh , K. R. 2005 . T cell responses to allogeneic human mesenchymal stem cells: Immunogenicity, tolerance, and suppression . Journal Biomedical Science , 12 : 47 – 57 .
   PubMed Web of Science ®Google Scholar
• Kong , H. J. , Kim , E. S. , Huang , Y. C. and Mooney , D. J. 2008 . Design of biodegradable hydrogel for the local and sustained delivery of angiogenic plasmid DNA . Pharmaceutical Research , 25 : 1230 – 1238 .
   PubMed Web of Science ®Google Scholar
• Krebs , M. D. , Salter , E. , Chen , E. , Sutter , K. A. and Alsberg , E. 2010 . Calcium phosphate-DNA nanoparticle gene delivery from alginate hydrogels induces in vivo osteogenesis . Journal of Biomedical Material Research A , 92 : 1131 – 1138 .
   PubMed Web of Science ®Google Scholar
• Lai , Q. G. , Yuan , K. F. , Xu , X. , Li , D. R. , Li , G. J. , Wei , F. L. , … and Li , S. 2011 . Transcription factor osterix modified bone marrow mesenchymal stem cells enhance callus formation during distraction osteogenesis . Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology , 111 : 412 – 419 .
   PubMedGoogle Scholar
• Lee , S. J. , Kang , S. W. , Do , H. J. , Han , I. , Shin , D. A. , Kim , J. H. and Lee , S. H. 2010 . Enhancement of bone regeneration by gene delivery of BMP2/Runx2 bicistronic vector into adipose-derived stromal cells . Biomaterials , 31 : 5652 – 5659 .
   PubMed Web of Science ®Google Scholar
• Lei , Y. , Huang , S. , Sharif-Kashani , P. , Chen , Y. , Kavehpour , P. and Segura , T. 2010 . Incorporation of active DNA/cationic polymer polyplexes into hydrogel scaffolds . Biomaterials , 31 : 9106 – 9116 .
   PubMed Web of Science ®Google Scholar
• Lim , K. I. 2012 . Retroviral integration profiles: Their determinants and implications for gene therapy . BMB Reports , 45 : 207 – 212 .
   PubMedGoogle Scholar
• Long , J. , Li , P. , Du , H. M. , Liu , L. , Zheng , X. H. , Lin , Y. F. , … and Tian , W. D. 2011 . Effects of bone morphogenetic protein 2 gene therapy on new bone formation during mandibular distraction osteogenesis at rapid rate in rabbits . Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology , 112 : 50 – 57 .
   PubMed Web of Science ®Google Scholar
• Lustig , L. R. and Akil , O. 2012 . Cochlear gene therapy . Current Opinion in Neurology , 25 : 57 – 60 .
   PubMedGoogle Scholar
• Madeira , C. , Mendes , R. D. , Ribeiro , S. C. , Boura , J. S. , Aires-Barros , M. R. , da Silva , C. L. and Cabral , J. M. 2010 . Nonviral gene delivery to mesenchymal stem cells using cationic liposomes for gene and cell therapy . Journal of Biomedicine and Biotechnology , 2010 : 735349 doi: 10.1155/2010/735349
   PubMedGoogle Scholar
• Martin , T. , Parker , S. E. , Hedstrom , R. , Le , T. , Hoffman , S. L. , Norman , J. , … and Lew , D. 1999 . Plasmid DNA malaria vaccine: the potential for genomic integration after intramuscular injection . Human Gene Therapy , 10 : 759 – 768 .
   PubMed Web of Science ®Google Scholar
• McCanless , J. D. , Jennings , L. K. , Cole , J. A. , Bumgardner , J. D. and Haggard , W. O. 2011 . In vitro differentiation and biocompatibility of mesenchymal stem cells on a novel platelet releasate-containing injectable composite . Journal Biomedical Material Research A , 100 : 220 – 229 .
   PubMedGoogle Scholar
• McKee , M. D. , Nakano , Y. , Masica , D. L. , Gray , J. J. , Lemire , I. , Heft , R. , … and Millán , J. L. 2011 . Enzyme replacement therapy prevents dental defects in a model of hypophosphatasia . Journal of Dental Research , 90 : 470 – 476 .
   PubMedGoogle Scholar
• Mehier-Humbert , S. and Guy , R. H. 2005 . Physical methods for gene transfer: improving the kinetics of gene delivery into cells . Advanced Drug Delivery Reviews , 57 : 733 – 753 .
   PubMed Web of Science ®Google Scholar
• Mingozzi , F. and High , K. A. 2012 . Therapeutic in vivo gene transfer for genetic disease using AAV: Progress and challenges . Nature Reviews Genetics , 12 : 341 – 355 .
   Google Scholar
• Mintzer , M. A. and Simanek , E. E. 2009 . Nonviral vectors for gene delivery . Chemistry Reviews , 109 : 259 – 302 .
   PubMed Web of Science ®Google Scholar
• Montiel-Equihua , C. A. , Thrasher , A. J. and Gaspar , H. B. 2012 . Gene therapy for severe combined immunodeficiency due to adenosine deaminase deficiency . Current Gene Therapy , 12 : 57 – 65 .
   PubMed Web of Science ®Google Scholar
• Moreira , C. , Oliveira , H. , Pires , L. R. , Simões , S. , Barbosa , M. A. and Pêgo , A. P. 2009 . Improving chitosan-mediated gene transfer by the introduction of intracellular buffering moieties into the chitosan backbone . Acta Biomaterials , 5 : 2995 – 3006 .
   PubMed Web of Science ®Google Scholar
• Park , D. J. , Choi , J. H. , Leong , K. W. , Kwon , J. W. and Eun , H. S. 2007 . Tissue-engineered bone formation with gene transfer and mesenchymal stem cells in a minimally invasive technique . Laryngoscope , 117 : 1267 – 1271 .
   PubMedGoogle Scholar
• Park , J. S. , Na , K. , Woo , D. G. , Yang , H. N. , Kim , J. M. , Kim , J. H. , Chung , H. M. and Park , K. H. 2010 . Non-viral gene delivery of DNA polyplexed with nanoparticles transfected into human mesenchymal stem cells . Biomaterials , 31 : 124 – 132 .
   PubMed Web of Science ®Google Scholar
• Park , S. , Kim , Y. S. , Kim , W. B. and Jon , S. 2009 . Carbon nanosyringe array as a platform for intracellular delivery . Nanotechnology Letters , 9 : 1325 – 1329 .
   Google Scholar
• Park , S. J. and Na , K. 2012 . The transfection efficiency of photosensitizer-induced gene delivery to human MSCs and internalization rates of EGFP and Runx2 genes . Biomaterials , 33 : 6485 – 6494 .
   PubMedGoogle Scholar
• Park , T. G. , Jeong , J. H. and Kim , S. W. 2006 . Current status of polymeric gene delivery systems . Advanced Drug Delivery Reviews , 58 : 467 – 486 .
   PubMed Web of Science ®Google Scholar
• Parveen , S. , Misra , R. and Sahoo , S. K. 2012 . Nanoparticles: a boon to drug delivery, therapeutics, diagnostics and imaging . Nanomedicine , 8 : 147 – 166 .
   PubMed Web of Science ®Google Scholar
• Reckhenrich , A. K. , Koch , C. , Egaña , J. T. and Plank , C. 2012 . The use of non-viral gene vectors for bioactive poly-(D, L-lactide) implant surfaces in bone tissue engineering . European Cells and Materials , 23 : 441 – 448 .
   PubMedGoogle Scholar
• Reiser , J. , Zhang , X. Y. , Hemenway , C. S. , Mondal , D. , Pradhan , L. and La Russa , V. F. 2005 . Potential of mesenchymal stem cells in gene therapy approaches for inherited and acquired diseases . Expert Opinion Biological Therapy , 5 : 1571 – 1584 .
   PubMed Web of Science ®Google Scholar
• Sakuma , T. , Barry , M. A. and Ikeda , Y. 2012 . Lentiviral vectors: Basic to translational . Biochemical Journal , 443 : 603 – 618 .
   PubMed Web of Science ®Google Scholar
• Santos , J. L. , Pandita , D. , Rodrigues , J. , Pêgo , A. P. , Granja , P. L. and Tomás , H. 2012 . Non-viral gene delivery to mesenchymal stem cells: Methods, strategies and application in bone tissue engineering and regeneration . Current Gene Therapy , 11 : 46 – 57 .
   Google Scholar
• Saraf , A. , Hacker , M. C. , Sitharaman , B. , Grande-Allen , K. J. , Barry , M. A. and Mikos , A. G. 2008 . Synthesis and conformational evaluation of a novel gene delivery vector for human mesenchymal stem cells . Biomacromolecules , 9 : 818 – 827 .
   PubMed Web of Science ®Google Scholar
• Shea , L. D. , Smiley , E. , Bonadio , J. and Mooney , D. J. 1999 . DNA delivery from polymer matrices for tissue engineering . Nature Biotechnology , 17 : 551 – 554 .
   PubMed Web of Science ®Google Scholar
• Sheyn , D. , Kallai , I. , Tawackoli , W. , CohnYakubovich , D. , Oh , A. , Su , S. , … and Gazit , D. 2011 . Gene modified adult stem cells regenerate vertebral bone defect in a rat model . Molecular Pharmaceutics , 8 : 1592 – 1601 .
   PubMed Web of Science ®Google Scholar
• Sheyn , D. , Pelled , G. , Zilberman , Y. , Talasazan , F. , Frank , J. M. , Gazit , D. and Gazit , Z. 2008 . Nonvirally engineered porcine adipose tissue-derived stem cells: Use in posterior spinal fusion . Stem cells , 26 : 1056 – 1064 .
   PubMed Web of Science ®Google Scholar
• Shimer , A. L. , Oner , F. C. and Vaccaro , A. R. 2009 . Spinal reconstruction and bone morphogenetic proteins: Open questions . Injury , 40 ( Suppl 3 ) : S32 – 38 .
   Google Scholar
• Smith , D. K. 2008 . Dendrimers and the double helix – From DNA binding towards gene therapy . Current Topics in Medicinal Chemistry , 8 : 1187 – 1203 .
   PubMed Web of Science ®Google Scholar
• Song , L. , Chau , L. , Sakamoto , Y. , Nakashima , J. , Koide , M. and Tuan , R. S. 2004 . Electric field-induced molecular vibration for noninvasive, high-efficiency DNA transfection . Molecular Therapy , 9 : 607 – 616 .
   PubMedGoogle Scholar
• Southwood , L. L. , Frisbie , D. D. , Kawcak , C. E. and McIlwraith , C. W. 2004 . Delivery of growth factors using gene therapy to enhance bone healing . Veterinary Surgery , 33 : 565 – 578 .
   PubMed Web of Science ®Google Scholar
• Su , C. H. , Wu , Y. J. , Wang , H. H. and Yeh , H. I. 2012 . Nonviral gene therapy targeting cardiovascular system . American Journal of Physiological Heart and Circulation Physiology , 303 : H629 – 638 .
   PubMed Web of Science ®Google Scholar
• Tachibana , R. , Harashima , H. , Ide , N. , Ukitsu , S. , Ohta , Y. , Suzuki , N. , … and Kiwada , H. 2002 . Quantitative analysis of correlation between number of nuclear plasmids and gene expression activity after transfection with cationic liposomes . Pharmaceutical Research , 19 : 377 – 381 .
   PubMedGoogle Scholar
• Thomas , C. E. , Ehrhardt , A. and Kay , M. A. 2003 . Progress and problems with the use of viral vectors for gene therapy . Nature Review Genetics , 4 : 346 – 358 .
   PubMed Web of Science ®Google Scholar
• Uchimura , E. , Yamada , S. , Uebersax , L. , Fujita , S. , Miyake , M. and Miyake , J. 2007 . Method for reverse transfection using gold colloid as a nano-scaffold . Journal Bioscience and Bioengineering , 103 : 101 – 103 .
   PubMedGoogle Scholar
• Warnock , J. N. , Daigre , C. and Al-Rubeai , M. 2011 . Introduction to viral vectors . Methods in Molecular Biology , 737 : 1 – 25 .
   PubMedGoogle Scholar
• Wegman , F. , Bijenhof , A. , Schuijff , L. , Oner , F. C. , Dhert , W. J. and Alblas , J. 2011 . Osteogenic differentiation as a result of BMP-2 plasmid DNA based gene therapy in vitro and in vivo . European Cells and Materials , 21 : 230 – 242 .
   PubMed Web of Science ®Google Scholar
• Wegman, F., Geuze, R. E., van der Helm, Y. J., Öner, F. C., Dhert, W. J., & Alblas, J. (2012). Gene delivery of bone morphogenetic protein-2 plasmid DNA promotes bone formation in a large animal model. Journal of Tissue Engineering and Regenerative Medicine. doi:10.1002/term.1571
   Google Scholar
• Wiethoff , C. M. and Middaugh , C. R. 2003 . Barriers to nonviral gene delivery . Journal of Pharmaceutical Science , 92 : 203 – 217 .
   PubMed Web of Science ®Google Scholar
• Wolff , J. A. and Rozema , D. B. 2008 . Breaking the bonds: Non-viral vectors become chemically dynamic . Molecular Therapy , 16 : 8 – 15 .
   PubMed Web of Science ®Google Scholar
• Yang , F. , Green , J. J. , Dinio , T. , Keung , L. , Cho , S. W. , Park , H. , Langer , R. and Anderson , D. G. 2009 . Gene delivery to human adult and embryonic cell-derived stem cells using biodegradable nanoparticulate polymeric vectors . Gene Therapy , 16 : 533 – 546 .
   PubMed Web of Science ®Google Scholar
• Yun , Y. R. , Jang , J. H. , Jeon , E. , Kang , W. , Lee , S. , Won , J. E. , … and Wall , I. 2012 . Administration of growth factors for bone regeneration . Regenerative Medicine , 7 : 369 – 385 .
   PubMed Web of Science ®Google Scholar
• Zhang , G. , Guo , B. , Wu , H. , Tang , T. , Zhang , B. T. , Zheng , L. , … and Qin , L. 2012 . A delivery system targeting bone formation surfaces to facilitate RNAi-based anabolic therapy . Nature Medicine , 18 : 307 – 314 .
   PubMed Web of Science ®Google Scholar