62
Views
0
CrossRef citations to date
0
Altmetric
Research Article

House dust mite allergen Der f 2 drives IL-6 and GM–CSF expression in airway epithelial cells via p38 MAPK/NF-κB signaling

, MD, , PhD, , MD, , MD & , PhD
Received 16 Mar 2024, Accepted 14 May 2024, Published online: 23 May 2024

References

  • Platts-Mills TA, de Weck AL. Dust mite allergens and asthma–a worldwide problem. J Allergy Clin Immunol. 1989;83(2 Pt 1):416–427.
  • Miller JD. The role of dust mites in allergy. Clin Rev Allergy Immunol. 2019;57(3):312–329. doi:10.1007/s12016-018-8693-0.
  • Thomas WR, Smith W-A, Hales BJ, Mills KL, O’Brien RM. Characterization and immunobiology of house dust mite allergens. Int Arch Allergy Immunol. 2002;129(1):1–18. doi:10.1159/000065179.
  • Wan H, Winton HL, Soeller C, Tovey ER, Gruenert DC, Thompson PJ, Stewart GA, Taylor GW, Garrod DR, Cannell MB, et al. Der p 1 facilitates transepithelial allergen delivery by disruption of tight junctions. J Clin Invest. 1999;104(1):123–133. doi:10.1172/JCI5844.
  • Hammad H, Lambrecht BN. Dendritic cells and epithelial cells: linking innate and adaptive immunity in asthma. Nat Rev Immunol. 2008;8(3):193–204. doi:10.1038/nri2275.
  • Asokananthan N, Graham PT, Stewart DJ, Bakker AJ, Eidne KA, Thompson PJ, Stewart GA. House dust mite allergens induce proinflammatory cytokines from respiratory epithelial cells: the cysteine protease allergen, Der p 1, activates protease-activated receptor (PAR)-2 and inactivates PAR-1. J Immunol. 2002;169(8):4572–4578. doi:10.4049/jimmunol.169.8.4572.
  • Trompette A, Divanovic S, Visintin A, Blanchard C, Hegde RS, Madan R, Thorne PS, Wills-Karp M, Gioannini TL, Weiss JP, et al. Allergenicity resulting from functional mimicry of a toll-like receptor complex protein. Nature. 2009;457(7229):585–588. doi:10.1038/nature07548.
  • Oh J-W, Kim E-Y, Koo B-S, Lee HB, Lee KS, Kim Y-S, Han J-S. Der f 2 activates phospholipase D in human T lymphocytes from Dermatophagoides farinae specific allergic individuals: involvement of protein kinase C-alpha. Exp Mol Med. 2004;36(5):486–492. doi:10.1038/emm.2004.61.
  • Ro EJ, Cha P-H, Kim H-Y, Cho Y-H, Park J-W, Han J-S, Choi K-Y. House dust mite allergen Der f 2 induces interleukin-13 expression by activating the PI3K/Akt pathway. Immunol Res. 2013;56(1):181–188. doi:10.1007/s12026-013-8392-0.
  • Wu CC, Liao EC, Lee MF, Tsai JJ. Augmentation of regulatory T cells in allergic individuals by recombinant Der f 2 peptide with fungal immunomodulatory peptide fve. Ann Allergy Asthma Immunol. 2009;102(3):216–222.
  • Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 2001;25(4):402–408. doi:10.1006/meth.2001.1262.
  • Marini M, Vittori E, Hollemborg J, Mattoli S. Expression of the potent inflammatory cytokines, granulocyte-macrophage-colony-stimulating factor and interleukin-6 and interleukin-8, in bronchial epithelial cells of patients with asthma. J Allergy Clin Immunol. 1992;89(5):1001–1009. doi:10.1016/0091-6749(92)90223-o.
  • Wang CB, Wong CK, Ip WK, Li MLY, Tian YP, Lam CWK. Induction of IL-6 in co-culture of bronchial epithelial cells and eosinophils is regulated by p38 MAPK and NF-kappaB. Allergy. 2005;60(11):1378–1385. doi:10.1111/j.1398-9995.2005.00884.x.
  • Krüll M, Bockstaller P, Wuppermann FN, Klucken AC, Mühling J, Schmeck B, Seybold J, Walter C, Maass M, Rosseau S, et al. Mechanisms of Chlamydophila pneumoniae-mediated GM-CSF release in human bronchial epithelial cells. Am J Respir Cell Mol Biol. 2006;34(3):375–382. doi:10.1165/rcmb.2004-0157OC.
  • Osterlund C, Grönlund H, Polovic N, Sundström S, Gafvelin G, Bucht A. The non-proteolytic house dust mite allergen Der p 2 induce NF-kappaB and MAPK dependent activation of bronchial epithelial cells. Clin Exp Allergy. 2009;39(8):1199–1208.
  • Hammad H, Lambrecht BN. The basic immunology of asthma. Cell. 2021;184(6):1469–1485. doi:10.1016/j.cell.2021.02.016.
  • Jerkic SP, Bachle L, Duecker RP, Gronau L, Chiocchetti AG, Zielen S, Schubert R. Association between polyunsaturated fatty acid profile and bronchial inflammation in bronchiolitis obliterans. Mediators Inflamm. 2023;2023:3406399.
  • Ordoñez C, Shaughnessy T, Matthay M, Fahy J. Increased neutrophil numbers and IL-8 levels in airway secretions in acute severe asthma: clinical and biologic significance. Am J Respir Crit Care Med. 2000;161(4):1185–1190. doi:10.1164/ajrccm.161.4.9812061.
  • Nocker RE, Schoonbrood DF, van de Graaf EA, Hack CE, Lutter R, Jansen HM, Out TA. Interleukin-8 in airway inflammation in patients with asthma and chronic obstructive pulmonary disease. Int Arch Allergy Immunol. 1996;109(2):183–191. doi:10.1159/000237218.
  • Fahy JV, Kim KW, Liu J, Boushey HA. Prominent neutrophilic inflammation in sputum from subjects with asthma exacerbation. J Allergy Clin Immunol. 1995;95(4):843–852. doi:10.1016/s0091-6749(95)70128-1.
  • Tscheppe A, Breiteneder H. Recombinant allergens in structural biology, diagnosis, and immunotherapy. Int Arch Allergy Immunol. 2017;172(4):187–202. doi:10.1159/000464104.
  • Cui Y, Yu L, Teng F, Wang N, Zhou Y, Zhang C, Yang L. Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems. Arch Med Sci. 2018;14(6):1348–1354.
  • Cao T, Zhang Z, Liu ZG, Dou X, Zhang J, Zhang W, Wu B, Yu ZD, Wei Z, Yu B. High-level expression and purification of the major house dust mite allergen Der p 2 in Escherichia coli. Protein Expr Purif. 2016;121:97–102.
  • Liu J-Y, Chen Y-C, Lin C-H, Kao S-H. Perilla frutescens leaf extract inhibits mite major allergen Der p 2-induced gene expression of pro-allergic and pro-inflammatory cytokines in human bronchial epithelial cell BEAS-2B. PLoS One. 2013;8(10):e77458. doi:10.1371/journal.pone.0077458.
  • Kurowska-Stolarska M, Kewin P, Murphy G, Russo RC, Stolarski B, Garcia CC, Komai-Koma M, Pitman N, Li Y, Niedbala W, et al. IL-33 induces antigen-specific IL-5+ T cells and promotes allergic-induced airway inflammation independent of IL-4. J Immunol. 2008;181(7):4780–4790. doi:10.4049/jimmunol.181.7.4780.
  • Soumelis V, Reche PA, Kanzler H, Yuan W, Edward G, Homey B, Gilliet M, Ho S, Antonenko S, Lauerma A, et al. Human epithelial cells trigger dendritic cell mediated allergic inflammation by producing TSLP. Nat Immunol. 2002;3(7):673–680. doi:10.1038/ni805.
  • Rincon M, Irvin CG. Role of IL-6 in asthma and other inflammatory pulmonary diseases. Int J Biol Sci. 2012;8(9):1281–1290.
  • Rose-John S, Jenkins BJ, Garbers C, Moll JM, Scheller J. Targeting IL-6 trans-signalling: past, present and future prospects. Nature Rev. 2023;23(10):666–681.
  • Tanaka T, Narazaki M, Kishimoto T. IL-6 in inflammation, immunity, and disease. Cold Spring Harb Perspect Biol. 2014;6(10):a016295.
  • Pan R, Kuai S, Li Q, Zhu X, Wang T, Cui Y. Diagnostic value of IL-6 for patients with asthma: a meta-analysis. Allergy Asthma Clin Immunol. 2023;19(1):39. doi:10.1186/s13223-023-00794-3.
  • Lee KMC, Achuthan AA, Hamilton JA. GM-CSF: A promising target in inflammation and autoimmunity. Immunotarget Ther. 2020;9:225–240.
  • Sheih A, Parks WC, Ziegler SF. GM-CSF produced by the airway epithelium is required for sensitization to cockroach allergen. Mucosal Immunol. 2017;10(3):705–715. doi:10.1038/mi.2016.90.
  • Saha S, Doe C, Mistry V, Siddiqui S, Parker D, Sleeman M, Cohen ES, Brightling CE. Granulocyte-macrophage colony-stimulating factor expression in induced sputum and bronchial mucosa in asthma and COPD. Thorax. 2009;64(8):671–676. doi:10.1136/thx.2008.108290.
  • Nobs SP, Pohlmeier L, Li F, Kayhan M, Becher B, Kopf M. GM-CSF instigates a dendritic cell-T-cell inflammatory circuit that drives chronic asthma development. J Allergy Clin Immunol. 2021;147(6):2118–2133 e3. doi:10.1016/j.jaci.2020.12.638.
  • Liu W, Liang Q, Balzar S, Wenzel S, Gorska M, Alam R. Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic airways. J Allergy Clin Immunol. 2008;121(4):893–902 e2. doi:10.1016/j.jaci.2008.02.004.
  • Escott KJ, Belvisi MG, Birrell MA, Webber SE, Foster ML, Sargent CA. Effect of the p38 kinase inhibitor, SB 203580, on allergic airway inflammation in the rat. Br J Pharmacol. 2000;131(2):173–176.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.