Establishment of a Rat Long-Term Culture Expressing the Osteogenic Phenotype: Dependence on Dexamethasone and FGF-2

References

• Owen, M. (1988). Marrow stromal stem cells. J. Cell Sci. Suppl. 10:63–76.
   PubMedGoogle Scholar
• Beresford, J.N. (1989). Osteogenic stem cells and the stromal system of bone and marrow. Clin. Orthop. Rel. Res. 240:270–280.
   PubMed Web of Science ®Google Scholar
• Owen, M., and Friedenstein, A.J. (1988). Stromal stem cells: marrow-derived osteogenic precursors. In: Cell and Molecular Biology of Vertebrate Hard Tissues (Ciba Found. Symp. 136), D. Evered and S. Harnett (eds.), pp. 42–60. (John Wiley & Sons, Chichester).
   Google Scholar
• Caplan, A.I. (1991). Mesenchymal stem cells. J. Orthop. Res. 9:641–650.
   PubMed Web of Science ®Google Scholar
• Bell, E. (1997). Tissue engineering in perspective. In: Principles of Tissue Engineering, R.P.L.R.V.J. Lanza (ed.), pp. xxxv–xli. (Academic Press, New York).
   Google Scholar
• Friedenstein, A.J. (1976). Precursor cells of mechanocytes. Int. Rev. Cytol. 47:327–359.
   PubMed Web of Science ®Google Scholar
• Friedenstein, A.J., Chailakhyan, R.K., and Gerasimov, U.V. (1987). Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers. Cell Tissue Kinet. 20:263–272.
   PubMedGoogle Scholar
• Maniatopoulos, C., Sodek, J., and Melcher, A.H. (1988). Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats. Cell Tissue Res. 254:317–330.
   PubMed Web of Science ®Google Scholar
• Bellows, C.G., Heersche, J.N.M., and Aubin, J.E. (1990). Determination of the capacity for proliferation and differentiation of osteoprogenitor cells in the presence and absence of dexamethasone. Dev. Biol. 140:132–138.
   PubMed Web of Science ®Google Scholar
• McCulloch, C.A.G., Strugurescu, M., Hughes, F., Melcher, A.H., and Aubin, J.E. (1991). Osteogenic progenitor cells in rat bone marrow stromal populations exhibit self-renewal in culture. Blood. 77:1906–1911.
   PubMed Web of Science ®Google Scholar
• Herbertson, A., and Aubin, J.E. (1995). Dexamethasone alters the subpopulation make-up of rat bone marrow stromal cell cultures. J. Bone Miner. Res. 10:285–294.
   PubMed Web of Science ®Google Scholar
• Shalhoub, V., Conlon, D., Tassinari, M., Quinn, C., Partridge, N., Stein, G.S., and Lian, J.B. (1992). Glucocorticoids promote development of the osteoblast phenotype by selectively modulating expression of cell growth and differentiation associated genes. J. Cell. Biochem. 50:425–440.
   PubMed Web of Science ®Google Scholar
• Rickard, D.J., Sullivan, T.A., Shenker, B.J., Leboy, P.S., and Kazhdan, I. (1994). Induction of rapid osteoblast differentiation in rat bone marrow stromal cell cultures by dexamethasone and BMP-2. Dev. Biol. 161:218–228.
   PubMed Web of Science ®Google Scholar
• Grigoriadis, A.E., Heersche, J.N.M., and Aubin, J.E. (1988). Differentiation of muscle, fat, cartilage, and bone from progenitor cells present in a bone-derived clonal cell population: Effect of dexamethasone. J. Cell. Biol. 106:2139–2151.
   PubMed Web of Science ®Google Scholar
• Kasugai, S., Todescan, R., Nagata, T., Yao, K.L., Butler, W.T., and Sodek, J. (1991). Expression of bone matrix proteins associated with mineralized tissue formation by adult rat bone marrow cells in vitro: Inductive effects of dexamethasone on the osteoblastic phenotype. J. Cell. Physiol. 147:111–120.
   PubMed Web of Science ®Google Scholar
• Malaval, L., Modrowski, D., Gupta, A.K., and Aubin, J.E. (1994). Cellular expression of bone-related proteins during in vitro osteogenesis in rat bone marrow stromal cell cultures. J. Cell. Physiol. 158:555–572.
   PubMed Web of Science ®Google Scholar
• Jaiswal, N., Haynesworth, S.E., Caplan, A.I., and Bruder, S.P. (1997). Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J. Cell. Biochem. 64:295–312.
   PubMed Web of Science ®Google Scholar
• Bruder, S.P., Jaiswal, N., and Haynesworth, S.E. (1997). Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation. J. Cell. Biochem. 64:278–294.
   PubMed Web of Science ®Google Scholar
• Tenenbaum, H.C., and Heersche, J.N.M. (1982). Differentiation of osteoblasts and formation of mineralized bone in vitro. Calcif. Tissue Int. 34:76–79.
   PubMed Web of Science ®Google Scholar
• Gospodarowicz, D., Ferrara, N., Schweigerer, L., and Neufeld, G. (1987). Structural characterization and biological functions of fibroblast growth factor. Endocr. Rev. 8:95–114.
   PubMed Web of Science ®Google Scholar
• Hauschka, P.V., Mavrakos, A.E., Iafrati, M.D., Doleman, S.E., and Klagsbrun, M. (1986). Growth factors in bone matrix: Isolation of multiple types by affinity chromatography on heparin sepharose. J. Biol. Chem. 261:12665–12674.
   PubMed Web of Science ®Google Scholar
• Kawaguchi, H., Kurikawa, T., Hanada, K., Hiyama, Y., Tamura, M., Ogata, E., and Matsumoto, T. (1994). Stimulation of fracture repair by recombinant human basic fibroblast growth factor in normal and streptozocindiabetic rats. Endocrinology 135:774–781.
   PubMed Web of Science ®Google Scholar
• Canalis, E., Centrella, M., and McCarthy, T. (1988). Effects of basic fibroblast growth factor on bone formation in vitro. J. Clin. Endocrinol. Metab. 81:1572–1577.
   Web of Science ®Google Scholar
• Rodan, S.B., Wesolowski, G., Thomas, K., and Rodan, G.A. (1987). Growth stimulation of rat calvaria osteoblastic cells by acidic fibroblast growth factor. Endocrinology 121:1917–1923.
   PubMed Web of Science ®Google Scholar
• Globus, R.K., Patterson-Buckendahl, P., and Gospodarowicz, D. (1988). Regulation of bovine bone cell proliferation by fibroblast growth factor and transforming growth factor. Endocrinology 123:98–105.
   PubMed Web of Science ®Google Scholar
• McCarthy, T.L., Centrella, M., and Canalis, E. (1989). Effects of fibroblast growth factors on deoxyribonucleic acid and collagen synthesis in rat parietal bone cells. Endocrinology 125:2118–2126.
   PubMed Web of Science ®Google Scholar
• Noff, D., Pitaru, S., and Savion, N. (1989). Basic fibroblast growth factor enhances the capacity of bone marrow cells to form bone-like nodules in vitro. FEBS Lett. 250:619–621.
   PubMed Web of Science ®Google Scholar
• Locklin, R.M., Williamson, M.C., Beresford, J.N., Triffitt, J.T., and Owen, M.E. (1995). In vitro effects of growth factors and dexamethasone on rat marrow stromal cells. Clin. Orthop. Rel. Res. 313:27–35.
   PubMed Web of Science ®Google Scholar
• Kuznetsov, S.A., Friedenstein, A.J., and Robey, P.G. (1997). Factors required for bone marrow stromal fibroblast colony formation in vitro. Br. J. Haematol. 97:561–570.
   PubMed Web of Science ®Google Scholar
• Rodan, S.B., Wesolowski, G., Yoon, K., and Rodan, G.A. (1989). Opposing effects of fibroblast growth factor and pertussis toxin on alkaline phosphatase, osteopontin, osteocalcin, and type I collagen mRNA levels in ROS 17/2.8 cells. J. Biol. Chem. 264:19934–19941.
   PubMed Web of Science ®Google Scholar
• Tang, K.T., Capparelli, C., Stein, J.L., Stein, G.S., Lian, J.B., Huber, A.C., Braverman, L.E., and DeVito, W.J. (1996). Acidic fibroblast growth factor inhibits osteoblast differentiation in vitro: Altered expression of collagenase, cell growth-related, and mineralization-associated genes. J. Cell. Biochem. 61:152–166.
   PubMed Web of Science ®Google Scholar
• Liang, K., Pun, S., and Wronski, T.J. (1999). Bone anabolic effects of basic fibroblast growth factor in ovariectomized rats. Endocrinology 140:5780–5788.
   PubMed Web of Science ®Google Scholar
• Pitaru, S., Kotev-Emeth, S., Noff, D., Kaffuler, S., and Savion, N. (1993). Effect of basic fibroblast growth factor on the growth and differentiation of adult stromal bone marrow cells: Enhanced development of mineralized bone-like tissue in culture. J. Bone Miner. Res. 8:919–929.
   PubMed Web of Science ®Google Scholar
• Pri-Chen, S., Pitaru, S., Lokiec, F., and Savion, N. (1998). Basic fibroblst growth factor enhances the growth and expression of the osteogenic phenotype of dexamethasone-treated human bone marrow derived bone-like cells in culture. Bone 23:111–117.
   PubMed Web of Science ®Google Scholar
• Gospodarowicz, D., Cheng, J., Lui, G.M., Baird, A., and Bohlent, P. (1984). Isolation of brain fibroblast growth factor by heparin-Sepharose affinity chromatography: Identity with pituitary fibroblast growth factor. Proc. Natl. Acad. Sci. USA 81:6963–6967.
   PubMed Web of Science ®Google Scholar
• Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal. Biochem. 72:248–254.
   PubMed Web of Science ®Google Scholar
• DiGirolamo, C.M., Stokes, D., Colter, D., Phinney, D.G., Class, R., and Prockop, D.J. (1999). Propagation and senescence of human marrow stromal cells in culture: A simple colony-forming assay identifies samples with the greatest potential to propagate and differentiate. Br. J. Haematol. 107:275–281.
   PubMed Web of Science ®Google Scholar
• Aubin, J.E. (1999). Osteoprogenitor cell frequency in rat bone marrow stromal populations: Role for heterotypic cell-cell interactions in osteoblast differentiation. J. Cell. Biochem. 72:396–410.
   PubMed Web of Science ®Google Scholar
• Martin, I., Muraglia, A., Campanile, G., Cancedda, R., and Quarto, R. (1997). Fibroblast growth factor-2 supports ex vivo expansion and maintenance of osteogenic precursors from human bone marrow. Endocrinology 138:4456–4462.
   PubMed Web of Science ®Google Scholar
• Locklin, R.M., Oreffo, R.O.C., and Triffitt, J.T. (1999). Effects of TGF beta and bFGF on the differentiation of human bone marrow stromal fibroblasts. Cell Biol. Int. 23:185–194.
   PubMed Web of Science ®Google Scholar
• Turksen, K., and Aubin, J.E. (1991). Positive and negative immunoselection for enrichment of two classes of osteoprogenitor cells. J. Cell. Biol. 114:373–384.
   PubMed Web of Science ®Google Scholar
• Aubin, J.E., and Heersche, J.N.M. (2000). Osteoprogenitor cell differentiation to mature bone-forming osteoblasts. Drug Dev. Res. 49:206–215.
   Web of Science ®Google Scholar
• Liu, F., Malaval, L., and Aubin, J.E. (1997). The mature osteoblast phenotype is characterized by extensive plasticity. Exp. Cell Res. 232:97–105.
   PubMed Web of Science ®Google Scholar
• Leis, H.J., Hulla, W., Gruber, R., Huber, E., Zach, D., Gleispach, H., and Windischhofer, W. (1997). Phenotypic heteogeneity of osteoblastlike MC3T3-E1 cells: Changes of bradykinin-induced prostaglandin E2 production during osteoblast maturation. J. Bone Miner. Res. 12:541–551.
   PubMedGoogle Scholar
• Kuznetsov, S.A., Krebsbach, P.H., Satomura, K., Kerr, J., Riminucci, M., Benayahu, D., and Robey, P.G. (1997). Single-colony derived strains of human marrow stromal fibroblasts form bone after transplantation in vivo. J. Bone Miner. Res. 12:1335–1347.
   PubMed Web of Science ®Google Scholar
• Yamaguchi, T.P., and Rossant, J. (1995). Fibroblast growth factors in mammalian development. Curr. Opin. Genet. Dev. 5:485–491.
   PubMed Web of Science ®Google Scholar