26
Views
0
CrossRef citations to date
0
Altmetric
Research Article

ZEB2 knockdown inhibits interleukin-1β-induced cartilage degradation and inflammatory response through the Wnt/β-catenin pathway in human chondrocytes

, , , , &
Received 10 Jan 2024, Accepted 20 May 2024, Published online: 20 Jun 2024

References

  • MacDonald KV, Sanmartin C, Langlois K, Marshall DA. Symptom onset, diagnosis and management of osteoarthritis. Health Rep 2014;25:10–17.
  • Pereira D, Ramos E, Branco J. Osteoarthritis. Acta Med Port 2015;28:99–106.
  • Conaghan PG, Abraham L, Viktrup L, Cappelleri JC, Beck C, Bushmakin AG, et al. Impact of osteoarthritis disease severity on treatment patterns and healthcare resource use: analysis of real-world data. Scand J Rheumatol 2023;52:353–63.
  • Roos EM, Arden NK. Strategies for the prevention of knee osteoarthritis. Nat Rev Rheumatol 2016;12:92–101.
  • Dinarello CA. A clinical perspective of IL-1β as the gatekeeper of inflammation. Eur J Immunol 2011;41:1203–17.
  • Al-Khazraji BK, Appleton CT, Beier F, Birmingham TB, Shoemaker JK. Osteoarthritis, cerebrovascular dysfunction and the common denominator of inflammation: a narrative review. Osteoarthr Cartil 2018;26:462–70.
  • Shen S, Guo J, Luo Y, Zhang W, Cui Y, Wang Q, et al. Functional proteomics revealed IL-1β amplifies TNF downstream protein signals in human synoviocytes in a TNF-independent manner. Biochem Biophys Res Commun 2014;450:538–44.
  • Frazer A, Bunning RA, Thavarajah M, Seid JM, Russell RG. Studies on type II collagen and aggrecan production in human articular chondrocytes in vitro and effects of transforming growth factor-beta and interleukin-1beta. Osteoarthr Cartil 1994;2:235–45.
  • Chen J, Gu YT, Xie JJ, Wu CC, Xuan J, Guo WJ, et al. Gastrodin reduces IL-1β-induced apoptosis, inflammation, and matrix catabolism in osteoarthritis chondrocytes and attenuates rat cartilage degeneration in vivo. Biomed Pharmacother 2018;97:642–51.
  • Cook AE, Cook JL, Stoker AM. Metabolic responses of meniscus to IL-1β. J Knee Surg 2018;31:834–40.
  • Zheng G, Zhan Y, Tang Q, Chen T, Zheng F, Wang H, et al. Monascin inhibits IL-1β induced catabolism in mouse chondrocytes and ameliorates murine osteoarthritis. Food Funct 2018;9:1454–64.
  • Zeng RM, Lu XH, Lin J, Hu J, Rong ZJ, Xu WC, et al. Knockdown of FOXM1 attenuates inflammatory response in human osteoarthritis chondrocytes. Int Immunopharmacol 2019;68:74–80.
  • Miyaura C, Inada M, Suzawa T, Sugimoto Y, Ushikubi F, Ichikawa A, et al. Impaired bone resorption to prostaglandin E2 in prostaglandin E receptor EP4-knockout mice. J Biol Chem 2000;275:19819–23.
  • Salvemini D, Misko TP, Masferrer JL, Seibert K, Currie MG, Needleman P. Nitric oxide activates cyclooxygenase enzymes. Proc Natl Acad Sci USA 1993;90:7240–4.
  • He Y, Moqbel SAA, Xu L, Ran J, Ma C, Xu K, et al. Costunolide inhibits matrix metalloproteinases expression and osteoarthritis via the NF-κB and Wnt/β-catenin signaling pathways. Mol Med Rep 2019;20:312–22.
  • Kobayashi M, Squires GR, Mousa A, Tanzer M, Zukor DJ, Antoniou J, et al. Role of interleukin-1 and tumor necrosis factor alpha in matrix degradation of human osteoarthritic cartilage. Arthritis Rheum 2005;52:128–35.
  • Katoh M, Katoh M. Integrative genomic analyses of ZEB2: transcriptional regulation of ZEB2 based on SMADs, ETS1, HIF1alpha, POU/OCT, and NF-kappaB. Int J Oncol 2009;34:1737–42.
  • Shen Z, Xuan W, Wang H, Sun F, Zhang C, Gong Q, et al. miR-200b regulates cellular senescence and inflammatory responses by targeting ZEB2 in pulmonary emphysema. Artif Cells Nanomed Biotechnol 2020;48:656–63.
  • Katsura A, Tamura Y, Hokari S, Harada M, Morikawa M, Sakurai T, et al. ZEB1-regulated inflammatory phenotype in breast cancer cells. Mol Oncol 2017;11:1241–62.
  • Xu H, Mei Q, Xiong C, Zhao J. Tumor-suppressing effects of miR-141 in human osteosarcoma. Cell Biochem Biophys 2014;69:319–25.
  • Lin H, Zheng X, Lu T, Gu Y, Zheng C, Yan H. The proliferation and invasion of osteosarcoma are inhibited by miR-101 via targetting ZEB2. Biosci Rep 2019;39(2):BSR20181283.
  • Aki T, Hashimoto K, Ogasawara M, Itoi E. A whole-genome transcriptome analysis of articular chondrocytes in secondary osteoarthritis of the hip. PLOS ONE 2018;13:e0199734.
  • Zhao GF, Huang LB. Zinc finger E-box binding homebox 2 alleviated experimental osteoarthritis in rats. Connect Tissue Res 2023;64:323–36.
  • Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method. Methods 2001;25:402–8.
  • Zhou L, Liu Z, Wang Z, Yu S, Long T, Zhou X, et al. Astragalus polysaccharides exerts immunomodulatory effects via TLR4-mediated MyD88-dependent signaling pathway in vitro and in vivo. Sci Rep 2017;7:44822.
  • Klatt AR, Paul-Klausch B, Klinger G, Kühn G, Renno JH, Banerjee M, et al. A critical role for collagen II in cartilage matrix degradation: collagen II induces pro-inflammatory cytokines and MMPs in primary human chondrocytes. J Orthop Res 2009;27:65–70.
  • Yao H, Hou G, Wang QY, Xu WB, Zhao HQ, Xu YC. LncRNA SPRY4-IT1 promotes progression of osteosarcoma by regulating ZEB1 and ZEB2 expression through sponging of miR-101 activity. Int J Oncol 2020;56:85–100.
  • Li SH, Fu J, Watkins DN, Srivastava RK, Shankar S. Sulforaphane regulates self-renewal of pancreatic cancer stem cells through the modulation of Sonic hedgehog–GLI pathway. Mol Cell Biochem 2013;373:217–27.
  • Hedbom E, Häuselmann HJ. Molecular aspects of pathogenesis in osteoarthritis: the role of inflammation. Cell Mol Life Sci 2002;59:45–53.
  • Ha C, Tian S, Sun K, Wang D, Lv J, Wang Y. Hydrogen sulfide attenuates IL-1β-induced inflammatory signaling and dysfunction of osteoarthritic chondrocytes. Int J Mol Med 2015;35:1657–66.
  • Yuan Y, Zhang GQ, Chai W, Ni M, Xu C, Chen JY. Silencing of microRNA-138-5p promotes IL-1β-induced cartilage degradation in human chondrocytes by targeting FOXC1: miR-138 promotes cartilage degradation. Bone Joint Res 2016;5:523–30.
  • Fei J, Liang B, Jiang C, Ni H, Wang L. Luteolin inhibits IL-1β-induced inflammation in rat chondrocytes and attenuates osteoarthritis progression in a rat model. Biomed Pharmacother 2019;109:1586–92.
  • Lei J, Fu Y, Zhuang Y, Zhang K, Lu D. miR-382-3p suppressed IL-1β induced inflammatory response of chondrocytes via the TLR4/MyD88/NF-κB signaling pathway by directly targeting CX43. J Cell Physiol 2019;234:23160–8.
  • Motta F, Barone E, Sica A, Selmi C. Inflammaging and osteoarthritis. Clin Rev Allergy Immunol 2023;64:222–38.
  • Chen ZL, Liu JY, Wang F, Jing X. Suppression of MALAT1 ameliorates chronic constriction injury-induced neuropathic pain in rats via modulating miR-206 and ZEB2. J Cell Physiol 2019;234:15647–53.
  • Ding Q, Wang Y, Zhang AL, Xu T, Zhou DD, Li XF, et al. ZEB2 attenuates LPS-induced inflammation by the NF-κB pathway in HK-2 cells. Inflammation 2018;41:722–31.
  • Lai Z, Cao Y. Plasma miR-200c-3p, miR-100-5p, and miR-1826 serve as potential diagnostic biomarkers for knee osteoarthritis: randomized controlled trials. Medicine (Baltimore) 2019;98:e18110.
  • Swahn H, Li K, Duffy T, Olmer M, D’Lima DD, Mondala TS, et al. Senescent cell population with ZEB1 transcription factor as its main regulator promotes osteoarthritis in cartilage and meniscus. Ann Rheum Dis 2023;82:403–15.
  • Yao Z, Qi W, Zhang H, Zhang Z, Liu L, Shao Y, et al. Down-regulated GAS6 impairs synovial macrophage efferocytosis and promotes obesity-associated osteoarthritis. Elife 2023;12:e83069.
  • Kim HR, Seo CW, Han SJ, Lee JH, Kim J. Zinc finger E-box binding homeobox 2 as a prognostic biomarker in various cancers and its correlation with infiltrating immune cells in ovarian cancer. Curr Issues Mol Biol 2022;44:1203–14.
  • Chen F, Ning Y, Liu J, Lian M, Wang J, Dan H. miR-147a targets ZEB2 to regulate ox-LDL-induced monocyte adherence to HUVECs, atherosclerotic plaque formation, and stability in atherosclerosis. J Biol Chem 2023;299:104657.
  • Huang JL, Liao Y, Qiu MX, Li J, An Y. Long non-coding RNA CCAT2 promotes cell proliferation and invasion through regulating Wnt/β-catenin signaling pathway in clear cell renal cell carcinoma. Tumour Biol 2017;39:1010428317711314.
  • Ding QH, Qi YY, Li XM, Chen WP, Wang XH, Ji XW. Knockdown of KIAA1199 suppresses IL-1β-induced cartilage degradation and inflammatory responses in human chondrocytes through the Wnt/β-catenin signalling pathway. Int Immunopharmacol 2019;73:203–11.
  • Zhou Y, Wang T, Hamilton JL, Chen D. Wnt/β-catenin signaling in osteoarthritis and in other forms of arthritis. Curr Rheumatol Rep 2017;19:53.
  • De Palma A, Nalesso G. WNT signalling in osteoarthritis and its pharmacological targeting. Handb Exp Pharmacol 2021;269:337–56.
  • Liu F, Li L, Lu W, Ding Z, Huang W, Li YT, et al. Scutellarin ameliorates cartilage degeneration in osteoarthritis by inhibiting the Wnt/β-catenin and MAPK signaling pathways. Int Immunopharmacol 2020;78:105954.
  • Chen J, Liu J, Chen S, Lai R, Zheng C, Lu J, et al. Salinomycin alleviates osteoarthritis progression via inhibiting Wnt/β-catenin signaling. Int Immunopharmacol 2022;112:109225.
  • Zhou DD, Wang X, Wang Y, Xiang XJ, Liang ZC, Zhou Y, et al. MicroRNA-145 inhibits hepatic stellate cell activation and proliferation by targeting ZEB2 through Wnt/β-catenin pathway. Mol Immunol 2016;75:151–60.
  • Weng Q, Chen Y, Wang H, Xu X, Yang B, He Q, et al. Dual-mode modulation of Smad signaling by Smad-interacting protein Sip1 is required for myelination in the central nervous system. Neuron 2012;73:713–28.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.