Real-time PCR-based quantification of Eimeria genomes: a method to outweigh underestimation of genome numbers due to PCR inhibition

References

• Aarthi , S. , Dhinakar Raj , G. , Raman , M. , Gomathinayagam , S. and Kumanan , K. 2010 . Molecular prevalence and preponderance of Eimeria spp. among chickens in Tamil Nadu, India . Parasitology Research , 107 : 1013 – 1017 . doi: 10.1007/s00436-010-1971-2
   PubMed Web of Science ®Google Scholar
• Blake , D.P. , Hesketh , P. , Archer , A. , Shirley , M.W. and Smith , A.L. 2006 . Eimeria maxima: the influence of host genotype on parasite reproduction as revealed by quantitative real-time PCR . International Journal for Parasitology , 36 : 97 – 105 . doi: 10.1016/j.ijpara.2005.09.011
   PubMed Web of Science ®Google Scholar
• Hartman , L.J. , Coyne , S.R. and Norwood , D.A. 2005 . Development of a novel internal positive control for Taqman based assays . Molecular and Cellular probes , 19 : 51 – 59 . doi: 10.1016/j.mcp.2004.07.006
   PubMed Web of Science ®Google Scholar
• Hodgson , J.N. 1970 . Coccidiosis: oocyst counting technique for coccidiostat evaluation . Experimental Parasitology , 28 : 99 – 102 . doi: 10.1016/0014-4894(70)90073-1
   PubMed Web of Science ®Google Scholar
• Long , P.L. , Joyner , L.P. , Millard , B.J. and Norton , C.C. 1976 . A guide to laboratory techniques used in the study and diagnosis of avian coccidiosis . Folia Veterinaria Latina , 6 : 201 – 217 .
   PubMedGoogle Scholar
• Morgan , J.A.T. , Morris , G.M. , Wlodek , B.M. , Byrnes , R. , Jenner , M. , Constantinoiu , C.C. , Anderson , G.R. , Lew-Tabor , A.E. , Molloy , J.B. , Gasser , R.B. and Jorgensen , W.K. 2009 . Real-time polymerase chain reaction (PCR) assays for the specific detection and quantification of seven Eimeria species that cause coccidiosis in chickens . Molecular and Cellular Probes , 23 : 83 – 89 . doi: 10.1016/j.mcp.2008.12.005
   PubMed Web of Science ®Google Scholar
• Novaes , J.L. , Rangel , T. , Ferro , M. , Abe , R.Y. , Manha , A.P. , de Mello , J.C. , Varuzza , L. , Durham , A.M. , Madeira , A.M. and Gruber , A. 2012 . A comparative transcriptome analysis reveals expression profiles conserved across three Eimeria spp. of domestic fowl and associated with multiple developmental stages . International Journal for Parasitology , 42 : 39 – 48 . doi: 10.1016/j.ijpara.2011.10.008
   PubMed Web of Science ®Google Scholar
• Sarfo , F.S. , Lavender , C.J. , Fyfe , J.A.M. , Johnson , P.D.R. , Stinear , T.P. and Phillips , T.O. 2011 . Mycobacterium ulcerans DNA not detected in faecal samples from Buruli ulcer patients: results of a pilot study . PLoS ONE , 6 : e19611 doi: 10.1371/journal.pone.0019611
   PubMed Web of Science ®Google Scholar
• Vrba , V. , Blake , D.P. and Poplstein , M. 2010 . Quantitative real-time PCR assays for detection and quantification of all seven Eimeria species that infect the chicken . Veterinary Parasitology , 174 : 183 – 190 . doi: 10.1016/j.vetpar.2010.09.006
   PubMed Web of Science ®Google Scholar