Polymerase chain reaction detection of greening bacterium (Candidatus Liberibacter asiaticus) and Citrus mosaic virus in citrus tissues, by means of a simplified template-preparation protocol

References

• Ahlawat , Y. S. 1997 . Virus, greening bacterium and viroids associated with citrus (Citrus species) decline in India . Indian J. Agric. Sci. , 67 : 51 – 57 .
   Google Scholar
• Ahlawat , Y. S. and Pant , R. P. 2004 . “ Major virus and virus-like diseases of citrus in India, their diagnosis and management ” . In Annual review of plant pathology , Edited by: Thind , T. S. , Gour , H. N. and Purohit , S. D. Vol. II , 447 – 474 . Jodhpur, , India : Scientific Publishers . Chap. 17
   Google Scholar
• Ahlawat , Y. S. , Pant , R. P. , Lockhart , B. E. L. , Srivastava , M. , Chakraborty , N. K. and Varma , A. 1996a . Association of a badnavirus with citrus mosaic disease in India . Plant Dis. , 80 : 590 – 592 .
   Google Scholar
• Ahlawat , Y. S. , Pant , R. P. , Shukla , A. and Lockhart , B. E. L. Partial characterization of a badnavirus associated with citrus yellow mosaic disease in India . Proceedings of the 13th Conference of the International Organization of Citrus Virologists (IOCV) . November 16–28 1995 , Fuzhu, China. Edited by: da Graça , J. V. , Moreno , P. and Yokomi , R. K. pp. 208 – 217 . Riverside, Calif : University of California .
   Google Scholar
• Baranwal , V. K. , Majumder , S. , Ahlawat , Y. S. and Singh , R. P. 2003 . Sodium sulphite yields improved DNA of higher stability for PCR detection of Citrus yellow mosaic virus from citrus leaves . J. Virol. Methods , 112 : 153 – 156 .
   PubMed Web of Science ®Google Scholar
• Baranwal , V. K. , Majumder , S. , Singh , J. , Suryanarayan , V. , Ghosh , D. K. and Ahlawat , Y. S. 2004 . PCR based detection of Candidatus liberibacter asiaticus, the agent of Huanglongbing or greening bacterium . Indian Phytopathol. , 52 : 164 – 168 .
   Google Scholar
• Baranwal , V. K. , Singh , J. , Ahlawat , Y. S. , Gopal , K. and Charaya , M. U. 2005 . Citrus yellow mosaic virus is associated with mosaic disease in Rangpur lime rootstock of citrus . Curr. Sci , 89 : 1596 – 1599 .
   Google Scholar
• Cambra , M. , Camarasa , E. , Gorris , M. T. , Garnsey , S. M. and Carbonell , E. Comparison of different immunosorbent assays for citrus tristeza virus (CTV) using CTV-specific monoclonal and polyclonal antibodies . Proceedings of the 11th Conference of the International Organization of Citrus Virologists (IOCV) . October 24–28 1989 , Florida, Calif. Edited by: Brlansky , R. H. , Lee , R. F. and Timme , L. W. pp. 38 – 45 . Riverside, Calif : University of California .
   Google Scholar
• da Graça , J. V. 1991 . Citrus greening disease . Annu. Rev. Phytopathol. , 29 : 109 – 136 .
   Web of Science ®Google Scholar
• Garnier , M. and Bové , J. M. Citrus greening disease and the greening bacterium . Proceedings of 12th Conference of the International Organization of Citrus Virologists (IOCV) . November 23–27 1992 , New Delhi, India. Edited by: Moreno , P. , da Graça , J. V. and Timmer , L. W. pp. 212 – 219 . Riverside, Calif : University of California .
   Google Scholar
• Garnsey , S. M. 2002 . Virological aspects to an integrated management of vector-borne virus diseases of citrus , Taipei, , Taiwan : Food and Fertilizer Technology Center for the Asian and Pacific Region . [online]
   Google Scholar
• Huang , Q. and Hartung , J. S. 2001 . Cloning and sequence analysis of an infectious clone of Citrus yellow mosaic virus that can infect sweet orange via Agrobacterium-mediated inoculation . J. Gen. Virol. , 82 : 2549 – 2558 .
   PubMedGoogle Scholar
• Hung , T. H. A. , Hung , S. C. , Chen , C. N. , Hsu , M. H. and Su , H. J. A. 2004 . Detection by PCR of Candidatus Liberibacter asiaticus, the bacterium causing citrus huanglongbing in vector psyllids; application to the study of vector-pathogen relationships . Plant Pathol. (London) , 53 : 96 – 102 .
   Web of Science ®Google Scholar
• Jagoueix , S. , Bové , J. M. and Garnier , M. 1996 . PCR detection of two “Candidatus” liberobacter species associated with greening disease of citrus . Mol. Cell. Probes , 10 : 43 – 50 .
   PubMed Web of Science ®Google Scholar
• James , D. 1999 . A simple and reliable protocol for the detection of apple stem grooving virus by RT-PCR and in a multiplex PCR assay . J. Virol. Methods , 83 : 1 – 9 .
   PubMedGoogle Scholar
• La Notte , P. , Minfra , A. and Saldarelli , P. 1997 . A spot-PCR technique for the detection of phloem-limited grapevine viruses . J. Virol. Methods , 66 : 103 – 108 .
   PubMedGoogle Scholar
• Li , W. , Hartung , J. S. and Levy , L. 2006 . Quantitative real-time PCR for detection and identification of Candidatus Liberibacter species associated with citrus huanglongbing . J. Microbiol. Methods , 66 : 101 – 115 .
   Google Scholar
• Olmos , A. , Dasi , M. A. , Candresse , T. and Cambra , M. 1996 . Print-capture PCR: a simple and highly sensitive method for the detection of plum pox virus (PPV) in plant tissues . Nucleic Acids Res. , 24 : 2192 – 2193 .
   PubMed Web of Science ®Google Scholar
• Reddy , B. V. B. 1997 . Characterization of citrus mosaic virus and to develop methods for its quick detection , New Delhi, , India : Indian Agricultural Research Institute . Ph.D. thesis
   Google Scholar
• Rowhani , A. , Maningas , M. A. , Lile , L. S. , Daubert , S. D. and Golino , D. A. 1995 . Development of a detection system for viruses of woody plants based on PCR analysis of immobilized virions . Phytopathology , 85 : 347 – 352 .
   Web of Science ®Google Scholar
• Rustici , G. , Accoto , G. P. , Noris , E. , Masenga , V. , Luisoni , E. and Milne , R. G. 2000 . Indian citrus ringspot virus: a proposed new species with some affinities to potex-, Carla-, fovea- and allexi viruses . Arch. Virol. , 145 : 1895 – 1908 .
   PubMedGoogle Scholar
• Sambrook , J. and Russell , D. W. 2001 . Molecular cloning: a laboratory manual , Cold Spring Harbor, N.Y : Cold Spring Harbor Laboratory Press .
   Google Scholar
• Singh , R. P. , Nie , X. , Singh , M. , Coffin , R. and Duplessis , P. 2002 . Sodium sulphite inhibition of potato and cherry polyphenolics in nucleic acid extraction for virus detection . J. Virol. Methods , 99 : 121 – 131 .
   Google Scholar
• Singh , R. P. , Dilworh , A. D. , Singh , M. and Mclaren , D. L. 2004 . Evaluation of a simple membrane-based nucleic acid preparation protocol for RT-PCR detection of potato viruses from aphid and plant tissues . J. Virol. Methods , 121 : 163 – 170 .
   PubMedGoogle Scholar
• Singh , R. P. , Dilworth , A. D. , Singh , M. and Babcock , K. M. 2006 . An alkaline solution simplifies nucleic acid preparation for RT-PCR and infectivity assay of viroids from crude sap and spotted membrane . J. Virol. Methods , 132 : 204 – 211 .
   PubMedGoogle Scholar
• Turturo , C. , Minafra , A. , Ni , H. , Wang , G. , Di Terlizzi , B. and Savino , V. 1998 . Occurrence of peach latent mosaic viroid in China and development of an improved detection method . J. Plant Pathol. , 80 : 165 – 169 .
   Google Scholar
• Weidemann , H. L. and Buchta , U. 1998 . A simple and rapid method for the detection of potato spindle tuber viroid (PSTVd) by RT-PCR . Potato Res. , 41 : 1 – 8 .
   Web of Science ®Google Scholar