Evaluation of Sulfur-Transfer Reagents for Large Scale Synthesis of Oligonucleotide Phosphorothioate Analogues

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• Beaucage reagent (1) was purchased from R. I. Chemical (Orange, CA); TETD (2) was from Applied Biosystems (Foster City, CA); dibenzoyl tetrasulfide (3)6 and bis(O, O-diisopropoxyphosphinothioyl) disulfide (S-Tetra, 4)7 were prepared by literature's procedure; benzyltriethyl-ammonium tetrathiomolybate (BTTM, 5) was provided by Aronex Pharmaceuticals (The Woodlands, TX); EDITH (6) was provided by PerSeptive Biosystems (Framingham, MA).
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