3′-Modified Nucleotides for DNA Sequencing Without Gel Electrophoresis

REFERENCES

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• 1H NMR (CDCl3) δ: 1.85 (d, 3H, Me-T, J6 Me = 0.79 Hz); 2.17 (dd, 1H, H′2, J2′-1′ = 5.52 Hz, J2′,2″ = 13.97 Hz); 2.34 (t, 2H, CH2); 3.49 (m, 2H, 2xCHOBn); 3.62 (m, 2H, CH2N3); 3.98 (m, 1H, H4′); 4.16 (m, 2H, H′5, H5″); 4.70 (d, 2H, CH2Ph); 4.57 (d, 2H, CH2Ph); 5.06 (m, 5H, H′3, 2xCH2Ph); 6.31 (dd, 1H, H′1, J1′,2′ = 5.35 Hz, J1′,2″ = 9.28 Hz); 7.35 (m, 21H, NH, 4xPh); 8.82 (d, 1H, H6). 13C NMR (CDCl3) δ: 12.19 (Me-T); 25.24 (C″2); 25.46 (C″3); 36.84 (C′2); 50.71 (C″6); 66.92 (C′5); 69.66 (2xCH2Ph); 72.40 (2xCH2Ph); 74.48 (C′3); 77.07 (C″5); 79.08 (C″4); 82.55 (d, C′4); 84.82 ((C′1); 111.62 (C5); 127.48–137.67 (C aromatics); 134.63 (C6); 150.20 (C2); 163.31 (C4); 172.76 (C=O). 31P NMR (CDCl3) δ: -2.50 (m, 1P)
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• 1H NMR (D2O) δ: 1.86 (s, 3H, Me-T); 3.22 (m, 2H, CH2NH2); 3.94 (m, 2H, H′5, H5″); 4.05 (m, 1H, H4′); 4.64 (m, 4H, 2xCH2Ph); 5.13 (m, IH, H′3); 6.11 (dd, 1H, H′1); 7.33 (s, 10H, 2xPh); 7.65 (s, 1H, H6). 31P NMR (D2O) δ: 0.78 (s, 1P)
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