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Original Articles

Concept of “Binary Oligonucleotide Reagent”

Pages 1969-1975 | Published online: 22 Aug 2006

REFERENCES

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  • Kazmierowski , H. G. , Neuland , P. , Laudmann , H. , Markwardt and Pharmazie , F. 1967 . 22 : 465 – 470 . 4-carbohydrazidebenzylamine was synthesized by Dr. V.I. Visochin in Prof. G.V. Shishkin's laboratory of the Novosibirsk Institute of Bioorganic Chemistry by a method similar to that described in It was chsracterized by UV and NMR spectra
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  • Linear gradient of potassium phosphate pH 7.5 from 0 to 0.2 M in 30% acetonitrile was used. The yields of derivatives Ia and Ib were about 40%. The yield of derivative II was 80%
  • Ghosh , S. S. , Kao , P. M. and Kwoh , D. Y. 1989 . Analyt. Biochem. , 178 : 43 – 51 . There are some additional bands on the electrophoretic picture. These materials moved faster than der. Ia (Ib). They may result from the incubation of der. Ia (Ib) in the T4 polynucleotide kinase reaction mixture. We suppose that they were 4-carboxybenzylamino derivatives of the corresponding oligonucleotides. These derivatives did not react with p-NBA, and their content estimated from the PAGE did not exceed 25%
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  • At concentrations of all components of the mixtures ten times as much as those the imine derivative formed in the absence of the target as effectively as in its presence
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