Antimicrobial Peptides from Plants

References

• Allen , A. K. , Desai , N. N. , Neuberger , A. and Creeth , J. M. 1978 . Properties of potato lectin and the nature of its glycoprotein linkages . Biochem. J. , 171 : 665 – 674 .
   PubMed Web of Science ®Google Scholar
• Andersen , N. H. , Cao , B. , Rodriguez-Romero , A. and Arreguin , B. 1993 . Hevein: NMR assignment and assessment of solution-state folding for the agglutinin-toxin motif . Biochemistry , 32 : 1407 – 1422 .
   PubMed Web of Science ®Google Scholar
• Andresen , I. , Becker , W. , Schluter , K. , Burges , J. , Parthier , B. and Apel , K. 1992 . The identification of leaf thionin as one of the main jasmonate-induced proteins of barley (Hordeum vulgare) . Plant Mol. Biol. , 19 : 193 – 204 .
   PubMed Web of Science ®Google Scholar
• Archer , B. L. , Audley , B. G. , Sweeney , G. and Hong , T. C. 1969 . Studies on composition of latex serum and “bottom fraction” particles . J. Rubber Res. Inst. Malaya , 21 : 560 – 569 .
   Google Scholar
• Arondel , V. and Kader , J. C. 1991 . Lipid transfer in plants . Experientia , 46 : 579 – 585 .
   Google Scholar
• Bevins , C. L. and Zasloff , M. 1990 . Peptides from frog skin . Annu. Rev. Biochem. , 59 : 395 – 414 .
   PubMed Web of Science ®Google Scholar
• Bloch , C. and Richardson , M. 1991 . A new family of small (5 kDa) protein inhibitors of insect α-amylases from seeds of sorghum (Sorghum bicolor (L) Moench) have sequence homologies with wheat γ-purothionins . FEBS Lett. , 279 : 101 – 104 .
   PubMed Web of Science ®Google Scholar
• Blochet , J. E. , Chevalier , C. , Forest , E. , Pebay-Peyroula , E. , Gautier , M. F. , Joudrier , P. , Pezolet , M. and Marion , D. 1993 . Complete amino acid sequence of puroindoline, a new basic and cystine-rich protein with a unique tryptophan-rich domain, isolated from wheat endosperm by Triton X-114 phase partitioning . FEBS Lett. , 329 : 336 – 340 .
   PubMed Web of Science ®Google Scholar
• Bohlmann , H. 1984 . The role of thionins in plant protection . Crit. Rev. Plant Sci. , 13 : 1 – 16 .
   Google Scholar
• Bohlmann , H. and Apel , K. 1987 . Isolation and characterization of cDNAs coding for leaf-specific thionins closely related to the endosperm-specific hordothionin of barley (Hordeum vulgare L.) . Mol. Gen. Genet. , 207 : 446 – 454 .
   Google Scholar
• Bohlmann , H. , Clausen , S. , Behnke , S. , Giese , H. , Hiller , C. , Reimann-Philipp , U. , Schrader , G. , Barkholt , V. and Apel , K. 1988 . Leaf-specific thionins of barley — a novel class of cell wall proteins toxic to plant-pathogenic fungi and possibly involved in the defense mechanism of plants . EMBO J. , 7 : 1559 – 1565 .
   PubMed Web of Science ®Google Scholar
• Boman , H. G. 1995 . Peptide antibiotics and their role in innate immunity . Annu. Rev. Immunol. , 13 : 61 – 92 .
   PubMed Web of Science ®Google Scholar
• Boman , H. G. and Hultmark , D. 1987 . Cell-free immunity in insects . Annu. Rev. Microbiol. , 41 : 103 – 126 .
   PubMed Web of Science ®Google Scholar
• Broekaert , W. F. , Cammue , B. P. A. , Osborn , R. W. and Rees , S. B. 1994 . Biocidal Chitin Binding Proteins International Patent Application WO94/11511
   Google Scholar
• Broekaert , W. F. , Lee , H.-I. , Kush , A. , Chua , N.-H. and Raikhel , N. V. 1990 . Wound-induced accumulation of mRNA containing a hevein sequence in laticifers of rubber tree (Hevea brasiliensis) . Proc. Natl. Acad. Sci. U.S.A. , 87 : 7633 – 7637 .
   PubMed Web of Science ®Google Scholar
• Broekaert , W. F. , Mariën , W. , Terras , F. R. G. , De Bolle , M. F. C. , Proost , P. , Van Damme , J. , Dillen , L. , Claeys , M. , Rees , S. B. , Vanderleyden , J. and Cammue , B. P. A. 1992 . Antimicrobial peptides from Amaranthus caudatus seeds with sequence homology to the cysteine/glycine-rich domain of chitin-binding proteins . Biochemistry , 31 : 4308 – 4314 .
   PubMed Web of Science ®Google Scholar
• Broekaert , W. F. , Terras , F. R. G. , Cammue , B. P. A. and Osborn , R. W. 1995 . Plant defensins: novel antimicrobial peptides as components of the host defence system . Plant Physiol. , 108 : 1353 – 1358 .
   PubMed Web of Science ®Google Scholar
• Bruix , M. , Gonzalez , C. , Santoro , J. , Soriano , F. , Rocher , A. , Mendez , E. and Rico , M. 1995 . 1H-NMR studies on the structure of a new thionin from barley endosperm . Biopolymers , 36 : 751 – 763 .
   PubMed Web of Science ®Google Scholar
• Bruix , M. , Jimenez , M. A. , Santora , J. , Gonzalez , C. , Colilla , F. J. , Mendez , E. and Rico , M. 1993 . Solution structure of γ1-H and γ1-P thionins from barley and wheat endosperm determined by 1H-NMR: a structural motif common to toxic arthropod proteins . Biochemistry , 32 : 715 – 724 .
   PubMed Web of Science ®Google Scholar
• Bunge , S. , Wolters , J. and Apel , K. 1992 . A comparison of leaf thionin sequences of barley cultivars and wild barley species . Mol. Gen. Genet. , 321 : 460 – 468 .
   Google Scholar
• Cammue , B. P. A. , De Bolle , M. F. C. , Terras , F. R. G. , Proost , P. , Van Damme , J. , Rees , S. B. , Vanderleyden , J. and Broekaert , W. F. 1992 . Isolation and characterization of a novel class of plant antimicrobial peptides from Mirabilis jalapa L. seeds . J. Biol. Chem. , 267 : 2228 – 2233 .
   PubMed Web of Science ®Google Scholar
• Cammue , B. P. A. , Thevissen , K. , Hendriks , M. , Eggermont , K. , Goderis , I. J. , Proost , P. , Van Damme , J. , Osborn , R. W. , Guerbette , F. , Kader , J. C. and Broekaert , W. F. 1995 . A potent antimicrobial protein from onion (Allium cepa L.) seeds showing sequence homology to plant lipid transfer proteins . Plant Physiol. , 109 : 445 – 455 .
   PubMed Web of Science ®Google Scholar
• Campos , F. A. P. and Richardson , M. 1984 . The complete amino acid sequence of the bifunctional α-amylase/trypsin inhibitor from seeds of ragi (Indian finger millet; Eleusine coracana Goertn.) . FEBS Lett. , 152 : 300 – 304 .
   Web of Science ®Google Scholar
• Carmona , M. J. , Hernandez-Lucas , C. , San Martin , C. , Gonzalez , P. and Garcia-Olmedo , F. 1993a . Sucellular localization of type I thionins in the endosperm of wheat and barley . Protoplasma , 173 : 1 – 7 .
   Web of Science ®Google Scholar
• Carmona , M. J. , Molina , A. , Fernandez , J. A. , Lopez-Fando , J. J. and Garcia-Olmedo , F. 1993b . Expression of the α-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens . Plant J. , 3 : 457 – 462 .
   PubMed Web of Science ®Google Scholar
• Castagnero , A. , Marana , C. , Carbonero , P. and Garcia-Olmedo , F. 1992 . Extreme divergence of a novel wheat thionin generated by a mutational burst specifically affecting the mature protein domain of the precursor . J. Mol. Biol. , 224 : 1003 – 1009 .
   PubMed Web of Science ®Google Scholar
• Castagnero , A. , Segura , A. and Garcia-Olmedo , F. 1995 . High conservation among sequences encoding type-V thionins in wheat and Aegilops . Plant Physiol. , 107 : 1475 – 1476 .
   PubMed Web of Science ®Google Scholar
• Casteels , P. , Romagnolo , J. , Castle , M. , Casteels-Josson , K. , Erdjument-Bromage , H. and Tempst , P. 1994 . Biodiversity of apidaecin-type peptide antibiotics. Prospects of manipulating the antibacterial spectrum and combatting acquired resistance . J. Biol. Chem. , 269 : 26107 – 26115 .
   PubMed Web of Science ®Google Scholar
• Casteels-Josson , K. , Capaci , T. , Casteels , P. and Tempst , P. 1993 . Apidaecin multipeptide precursor structure: a putative mechanism for amplification of the insect antibacterial response . EMBO J. , 12 : 1569 – 1578 .
   PubMed Web of Science ®Google Scholar
• Chagolla-Lopez , A. , Blanco-Labra , A. , Patthy , A. , Sanchez , R. and Ponger , S. 1994 . A novel α-amylase inhibitor from amaranth (Amaranthus hypocondriacus) seeds . J. Biol. Chem. , 269 : 23675 – 23680 .
   PubMed Web of Science ®Google Scholar
• Chiang , C. C. and Hadwiger , L. A. 1991 . The Fusarium solani-indaced expression of a pea gene family encoding high cysteine content proteins . Mol. Plant Microbe Interact , 4 : 324 – 331 .
   PubMed Web of Science ®Google Scholar
• Clark , A. M. and Bohnert , H. J. 1993 . Epidermis-specific transcripts. Nucleotide sequence of a full-length cDNA of EPI12, encoding a putative lipid transfer protein . Plant Physiol. , 103 : 677 – 678 .
   PubMed Web of Science ®Google Scholar
• Clore , G. M. , Nilges , M. , Sukumaran , D. K. , Brünger , A. T. , Karplus , M. and Gronenborn , A. M. 1986 . The three-dimensional structure of α-purothionin in solution: combined use of nuclear magnetic resonance, distance geometry and restrained molecular dynamics . EMBO J. , 5 : 2729 – 2735 .
   PubMed Web of Science ®Google Scholar
• Clore , G. M. , Sukumaran , D. K. , Nilges , M. and Gronenborn , A. M. 1987 . Three-dimensional structure of phoratoxin in solution: combined use of nuclear magnetic resonance, distance geometry, and restrained molecular dynamics . Biochemistry , 26 : 1732 – 1745 .
   Web of Science ®Google Scholar
• Cociancich , S. , Bulet , P. , Hétru , C. and Hoffmann , J. A. 1994 . The inducible antibacterial peptides of insects . Parasitol. Today , 10 : 132 – 139 .
   PubMedGoogle Scholar
• Colilla , F. J. , Rocher , A. and Mendez , E. 1990 . γ-Purothionins: amino acid sequence of two polypeptides of a new family of thionins from wheat endosperm . FEBS Lett. , 270 : 191 – 194 .
   PubMed Web of Science ®Google Scholar
• Coulson , E. J. , Harris , T. H. and Axelrod , B. 1942 . Effect on small laboratory animals of the injection of the crystalline hydrochloride of a sulfur protein from wheat flour . Cereal Chem. , 19 : 301 – 307 .
   Google Scholar
• Creelman , R. A. , Tierney , M. L. and Mullet , J. E. 1992 . Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression . Proc. Natl. Acad. Sci. USA , 89 : 4938 – 4941 .
   PubMed Web of Science ®Google Scholar
• De Bolle , M. F. C. , David , K. M. M. , Rees , S. B. , Vanderleyden , J. , Cammue , B. P. A. and Broekaert , W. F. 1993 . Cloning and characterization of a cDNA encoding an antimicrobial chitin-binding protein from amaranth . Amaranthus caudatus. Plant Mol. Biol. , 22 : 1187 – 1190 .
   PubMed Web of Science ®Google Scholar
• De Bolle , M. F. C. , Eggermont , K. , Duncan , R. E. , Osborn , R. W. , Terras , F. R. G. and Broekaert , W. F. 1995 . Cloning and characterization of two cDNA clones encoding seed-specific antimicrobial peptides from Mirabilis jalapa L . Plant Mol. Biol. , 28 : 713 – 721 .
   PubMed Web of Science ®Google Scholar
• De Bolle , M. F. C. , Osborn , R. W. , Goderis , I. J. , Noe , L. , Acland , D. , Hart , C. A. , Torrekens , S. , Van Leuven , F. and Broekaert , W. F. 1996 . Antimicrobial peptides from Mirabilis jalapa and Amaranthus caudatus: expression, processing, localization and biological activity in transgenic tobacco . Plant Mol. Biol. , 31 : 993 – 1008 .
   PubMed Web of Science ®Google Scholar
• De Samblanx , G. W. , Goderis , I. J. , Thevissen , K. , Raemaekers , R. , Fant , F. , Borremans , F. , Acland , D. , Osborn , R. W. , Patel , S. and Broekaert , W. F. 1997 . Mutational analysis of a plant defensin from radish (Raphamus sativus L.) reveals two adjacent sites important for antifungal activity . J. Biol. Chem. , 272 : 1171 – 1179 .
   PubMed Web of Science ®Google Scholar
• Diamond , G. , Jones , D. E. and Bevins , C. L. 1993 . Airway epithelial cells are the site of expression of a mammalian antimicrobial peptide gene . Proc. Natl. Acad. Sci. U.S.A. , 90 : 4596 – 4600 .
   PubMed Web of Science ®Google Scholar
• Domon , C. , Evrard , J. L. , Herdenberger , F. , Pillay , D. T. N. and Steinmetz , A. 1990 . Nucleotide sequence of two anther-specific cDNAs from sunflower (Helianthus annuus L.) . Plant Mol. Biol. , 15 : 643 – 646 .
   PubMed Web of Science ®Google Scholar
• Duclohier , H. 1994 . Anion pores from magainins and related defensive peptides . Toxicology , 87 : 175 – 188 .
   PubMed Web of Science ®Google Scholar
• Duvick , J. P. , Rood , T. , Rao , A. G. and Marshak , D. R. 1992 . Purification and characterization of a novel antimicrobial peptide from maize (Zea mays L.) kernels . J. Biol. Chem. , 267 : 18814 – 18820 .
   PubMed Web of Science ®Google Scholar
• Epple , P. , Apel , K. and Bohlmann , H. 1995 . An Arabidopsis thaliana thionin gene is inducible via a signal transduction pathway different from that for pathogenesis-related proteins . Plant Physiol. , 109 : 813 – 820 .
   PubMed Web of Science ®Google Scholar
• Evett , G. E. , Donaldson , D. M. and Vernon , L. P. 1986 . Biological properties of Pyrularia thionin prepared from nuts of Pyrularia pubera . Toxicon , 24 : 622 – 625 .
   PubMed Web of Science ®Google Scholar
• Fant , F. , Vranken , W. F. , Martins , J. C. and Borremans , F. A. M. 1996 . Solution conformation of Raphanus sativus antifungal protein 1 (Rs-AFP1) by 1H NMR. Resonance assignment, secondary structure and global fold . Bull. Soc. Chim. Belg. , 106 : 51 – 57 .
   Google Scholar
• Farmer , E. E. and Ryan , C. A. 1992 . Octadecanoid precursors of jasmonic acid activate the synthesis of wound-inducible proteinase inhibitors . Plant Cell , 4 : 129 – 134 .
   PubMed Web of Science ®Google Scholar
• de Caleya , R. Fernandez , Gonzalez-Pascual , B. , Garcia-Olmedo , F. and Carbonero , P. 1972 . Susceptibility of phytopathogenic bacteria to wheat purothionins in vitro . Appl. Microbiol. , 23 : 998 – 1000 .
   PubMedGoogle Scholar
• Fleming , A. J. , Mandel , T. , Hofmann , S. , de Vries , S. C. and Kuhlemeier , K. 1992 . Expression pattern of a tobacco lipid transfer protein gene within the shoot apex . Plant J. , 2 : 855 – 862 .
   PubMed Web of Science ®Google Scholar
• Florack , D. E. A. 1994 . Application of Hordo-thionins and Cecropin B for Engineering Bacterial Disease Resistance into Plants , Ph.D. thesis The Netherlands : Landbouwuniversiteit Wageningen .
   Google Scholar
• Florack , D. E. A. , Dirkse , W. G. , Visser , B. , Heidekamp , F. and Stiekema , W. J. 1994 . Expression of biologically active hordothionins in tobacco. Effects of pre- and pro-sequences at the amino- and carboxyl-termini of the hordothionin precursor on mature protein expression and sorting . Plant Mol. Biol. , 24 : 83 – 96 .
   PubMed Web of Science ®Google Scholar
• Florack , D. E. A. and Stiekema , W. J. 1994 . Thionins: properties, possible biological roles and mechanisms of action . Plant Mol. Biol. , 26 : 25 – 37 .
   PubMed Web of Science ®Google Scholar
• Florack , D. E. A. , Visser , B. , De Vries , P. M. , Van Vuurde , J. W. L. and Stiekema , W. J. 1993 . Analysis of the toxicity of purothionins and hordothionins for plant pathogenic bacteria . Neth. J. Plant Pathol. , 99 : 259 – 268 .
   Web of Science ®Google Scholar
• Ganz , T. and Lehrer , R. I. 1995 . Defensins . Immunology , 6 : 584 – 589 .
   Google Scholar
• Ganz , T. , Metcalf , J. A. , Gallin , J. I. , Boxer , L. A. and Lehrer , R. I. 1988 . Microbicidal/cytotoxic proteins of neutrophils are deficient in two disorders: Chédiak-Higashi syndrome and “specific” granule deficiency . J. Clin. Invest. , 82 : 552 – 556 .
   PubMed Web of Science ®Google Scholar
• Garcia-Olmedo , F. , Molina , A. , Segura , A. and Moreno , M. 1995 . The defensive role of nonspecific lipid-transfer proteins in plants . Trends Microbiol. , 3 : 72 – 74 .
   PubMedGoogle Scholar
• Garcia-Olmedo , F. , Salcedo , G. , Sanchez-Monge , R. , Hernandez-Lucas , C. , Carmona , M. J. , Lopez-Fando , J. J. , Fernandez , J. A. , Gomez , L. , Royo , J. , Garcia-Maroto , F. , Castagnaro , A. and Carbonero , P. 1992 . “ Trypsin/α-amylase inhibitors and thionins: possible defence proteins from barley ” . In Barley: Genetics, Biochemistry, Molecular Biology and Biotechnology , Edited by: Shewry , P. R. 335 – 350 . Wallingford : CAB International .
   Google Scholar
• Gasanov , S. E. , Vernon , L. P. and Aripov , T. F. 1993 . Modification of phospholipid membrane structure by the plant toxic peptide Pyrularia thionin . Arch. Biochem. Biophys , 301 : 367 – 374 .
   PubMed Web of Science ®Google Scholar
• Gausing , K. 1987 . Thionin genes specifically expressed in barley leaves . Planta , 171 : 241 – 246 .
   PubMed Web of Science ®Google Scholar
• Gausing , K. 1994 . Lipid transfer protein genes specifically expressed in barley leaves and coleoptiles . Planta , 192 : 574 – 580 .
   PubMed Web of Science ®Google Scholar
• Gautier , M. F. , Aleman , M. E. , Guirao , A. , Marion , D. and Joudrier , P. 1994 . Triticum aestivum puroindolines, two basic cystine-rich seed proteins: cDNA sequence analysis and developmental gene expression . Plant Mol. Biol. , 25 : 43 – 57 .
   PubMed Web of Science ®Google Scholar
• Gincel , E. , Simorre , J. P. , Caille , A. , Marion , D. , Ptak , M. and Vovelle , F. 1994 . Three-dimensional structure in solution of a wheat lipid-transfer protein from multidimensional 1H-NMR data. A new folding for lipid carriers . Eur. J. Biochem. , 226 : 413 – 422 .
   PubMedGoogle Scholar
• Groisman , E. A. , Parra-Lopez , C. , Salcedo , M. , Lipps , C. J. and Heffron , F. 1992 . Resistance to host antimicrobial peptides is necessary for Salmonella virulence . Proc. Natl. Acad. Sci. U.S.A. , 89 : 11939 – 11943 .
   PubMed Web of Science ®Google Scholar
• Gu , Q. , Kawata , E. E. , Morse , M.-J. , Wu , H.-M. and Cheung , A. Y. 1992 . A flower-specific cDNA encoding a novel thionin in tobacco . Mol. Gen. Genet. , 234 : 89 – 96 .
   PubMedGoogle Scholar
• Guihard , G. , Bénédetti , H. , Besnard , M. and Letellier , L. 1993 . Phosphate efflux through the channels formed by colicins and phage T5 in Escherichia coli cells is responsible for the fall in cytoplasmic ATP . J. Biol. Chem. , 268 : 17775 – 17780 .
   PubMed Web of Science ®Google Scholar
• Hendrickson , W. A. and Teeter , A. M. 1981 . Structure of the hydrophobic protein crambin determined directly from the anomalous scattering of sulphur . Nature , 290 : 107 – 113 .
   PubMed Web of Science ®Google Scholar
• Hoffmann , J. A. and Hétru , C. 1992 . Insect defensins: inducible antibacterial peptides . Immunol. Today , 13 : 411 – 415 .
   PubMedGoogle Scholar
• Iseli , B. , Boller , T. and Neuhaus , J. M. 1993 . The N-terminal cysteine-rich domain of tobacco class I chitinase is essential for chitin binding but not for catalytic or antifungal activity . Plant Physiol. , 103 : 221 – 226 .
   PubMed Web of Science ®Google Scholar
• Ishibashi , N. , Yamauchi , D. and Minamikawa , T. 1990 . Stored mRNA in cotyledons of Vigna unguiculata seeds: nucleotide sequence of cloned cDNA for a stored mRNA and induction of its synthesis bv. precocious germination . Plant Mol. Biol. , 15 : 59 – 64 .
   PubMed Web of Science ®Google Scholar
• José-Estanyol , M. , Ruiz-Avilla , L. and Puigdomenech , P. 1992 . A maize embryo-specific gene encodes a proline-rich and hydrophobic protein . Plant Cell , 4 : 413 – 423 .
   PubMed Web of Science ®Google Scholar
• Kader , J. C. , Julienne , M. and Vergnolle , C. 1984 . Purification and characterization of a spinach-leaf protein capable of transferring phospholipids from liposomes to mitochondria or chloroplasts . Eur. J. Biochem. , 139 : 411 – 416 .
   PubMedGoogle Scholar
• Karunanandaa , B. , Singh , A. and Kao , T.-H. 1994 . Characterization of a predominantly pistil-expressed gene encoding a gamma-thionin-like protein of Petunia inflata . Plant Mol. Biol. , 26 : 459 – 464 .
   PubMed Web of Science ®Google Scholar
• Kieliszewski , M. J. , Showalter , A. M. and Leykam , J. F. 1994 . Potato lectin: a modular protein sharing sequence similarities with the extensin family, the hevein lectin family, and snake venom disintegrins (platelet aggregation inhibitors) . Plant J. , 5 : 849 – 861 .
   PubMed Web of Science ®Google Scholar
• Klessig , D. F. and Malamy , J. 1994 . The salicylic acid signal in plants . Plant Mol. Biol. , 26 : 1439 – 1458 .
   PubMed Web of Science ®Google Scholar
• Kobayashi , Y. , Sato , A. , Takashima , H. , Tamaoki , H. , Nishimura , S. , Kyogoku , Y. , Ikenaka , K. , Kondo , T. , Mikoshiba , K. , Hojo , H. , Aimoto , S. and Moroder , L. 1991 . A new α-helical motif in membrane active peptides . Neurochem. Int. , 18 : 525 – 534 .
   PubMed Web of Science ®Google Scholar
• Koltunow , A. M. , Truettner , J. , Cox , K. H. , Wallroth , M. and Goldberg , R. B. 1990 . Different temporal and spatial expression patterns occur during anther development . Plant Cell , 2 : 1201 – 1224 .
   PubMed Web of Science ®Google Scholar
• Kragh , K. M. , Nielsen , J. E. , Nielsen , K. K. , Dreboldt , S. and Mikkelsen , J. D. 1995 . Characterisation and localisation of new antifungal cysteine-rich proteins from Beta vulgaris . Mol. Plant Microbe Interact , 8 : 424 – 434 .
   PubMed Web of Science ®Google Scholar
• Kramer , K. J. , Klassen , L. W. , Jones , B. L. , Speirs , R. D. and Kammer , A. E. 1979 . Toxicity of purothionin and its homologues to the tobacco hornworm, Manduca sexta (L.) (Lepidoptera: Sphingidae) . Toxicol. Appl. Pharmacol. , 48 : 179 – 183 .
   PubMed Web of Science ®Google Scholar
• Kraulis , P. J. , Clore , G. M. , Nilges , M. , Jones , T. A. , Pettersson , G. , Knowles , J. and Gronenborn , A. M. 1989 . Determination of the three-dimensional solution structure of the C-terminal domain of cellobiohydrolase I from Trichoderma reesei. A study using nuclear magnetic resonance and hybrid distance geometry-dynamic simulated annealing . Biochemistry , 28 : 7241 – 7257 .
   PubMed Web of Science ®Google Scholar
• Lee , H.-I. , Broekaert , W. F. and Raikhel , N. V. 1991 . Co- and post-translational processing of the hevein preproprotein of latex of the rubber tree (Hevea brasiliensis) . J. Biol. Chem. , 266 : 15944 – 15948 .
   PubMed Web of Science ®Google Scholar
• Lee , H. I. and Raikhel , N. V. 1995 . Prohevein is poorly processed but shows enhanced resistance to a chitin-binding fungus in transgenic tomato plants . Braz. J. Med. Biol. Res. , 28 : 743 – 750 .
   PubMed Web of Science ®Google Scholar
• Lee , J. Y. , Boman , A. , Chaunxin , S. , Andersson , M. , Jornvall , H. , Mutt , V. and Boman , H. G. 1989 . Antibacterial peptides from pig intestine: isolation of a mammalian cecropin . Proc. Natl. Acad. Sci. U.S.A. , 86 : 9159 – 9162 .
   PubMed Web of Science ®Google Scholar
• Lehrer , R. I. , Ganz , T. and Selsted , M. E. 1991 . Defensins: endogenous antibiotic peptides of animal cells . Cell , 64 : 229 – 230 .
   PubMed Web of Science ®Google Scholar
• Lemaitre , B. , Kromer-Metzger , E. , Michaut , L. , Nicolas , E. , Meister , M. , Georgel , P. , Reichhart , J.-M. and Hoffmann , J. A. 1995 . A recessive mutation, immune deficiency (imd), defines two distinct control pathways in the Drosophila host defence . Proc. Natl. Acad. Sci. U.S.A. , 92 : 9465 – 9469 .
   PubMed Web of Science ®Google Scholar
• Le-Nguyen , D. L. , Heitz , A. , Chiche , L. , Castro , B. , Baigegrain , R. , Favel , A. and Coletti-Previero , M. 1990 . Molecular recognition between serine proteases and new bioactive micro-proteins with a knotted structure . Biochimie , 72 : 431 – 435 .
   PubMed Web of Science ®Google Scholar
• Matsuzaki , K. , Murase , O. , Fujii , N. and Miyajima , K. 1995 . Translocation of a channel-forming antimicrobial peptide, magainin 2, across lipid bilayers by forming a pore . Biochemistry , 34 : 6521 – 6526 .
   PubMed Web of Science ®Google Scholar
• Mellstrand , S. T. and Samuelsson , G. 1973 . Phoratoxin, a toxic protein from the mistletoe Phoradendron tomentosum subsp. macrophyllum (Loranthaceae). Improvements in the isolation procedure and further studies on the properties . Eur. J. Biochem. , 32 : 143 – 147 .
   PubMedGoogle Scholar
• Mendez , E. , Moreno , A. , Colilla , F. , Pelaez , F. , Limas , G. G. , Mendez , R. , Soriano , F. , Salinas , M. and De Haro , C. 1990 . Primary structure and inhibition of protein synthesis in eukaryotic cell-free system of a novel thionin, γ-hordothionin, from barley endosperm . Eur. J. Biochem. , 194 : 533 – 539 .
   PubMedGoogle Scholar
• Milligan , S. B. and Gasser , C. S. 1995 . Nature and regulation of pistil-expressed genes in tomato . Plant Mol. Biol. , 28 : 691 – 711 .
   PubMed Web of Science ®Google Scholar
• Molina , A. and Garcia-Olmedo , F. 1993 . Developmental and pathogen-induced expression of three barley genes encoding lipid transfer proteins . Plant J. , 4 : 983 – 991 .
   PubMed Web of Science ®Google Scholar
• Molina , A. , Goy , P. A. , Fraile , A. , Sanchez-Monge , R. and Garcia-Olmedo , F. 1993a . Inhibition of bacterial and fungal plant pathogens by thionins of types I and II . Plant Sci. , 92 : 169 – 177 .
   Web of Science ®Google Scholar
• Molina , A. , Segura , A. and Garcia-Olemdo , F. 1993b . Lipid transfer proteins (nsLTPs) from barley and maize leaves are potent inhibitors of bacterial and fungal plant pathogens . FEBS Lett. , 316 : 119 – 122 .
   PubMed Web of Science ®Google Scholar
• Moreno , M. , Segura , A. and Garcia-Olmedo , F. 1994 . Pseudothionin-St1, a potato peptide active against potato pathogens . Eur. J. Biochem. , 223 : 135 – 139 .
   PubMedGoogle Scholar
• Mueller , M. J. , Brodschelm , W. , Spannagl , E. and Zenk , M. H. 1993 . Signaling in the elicitation process is mediated through the octadecanoid pathway leading to jasmonic acid . Proc. Natl. Acad. Sci. U.S.A. , 90 : 7490 – 7497 .
   PubMed Web of Science ®Google Scholar
• Nicolas , P. and Mor , A. 1995 . Peptides as weapons against microorganisms in the chemical defense system of vertebrates . Annu. Rev. Microbiol. , 49 : 277 – 304 .
   PubMed Web of Science ®Google Scholar
• Nürnberger , T. , Nennstiel , D. , Jabs , T. , Sacks , W. R. , Hahlbrock , K. and Scheel , D. 1994 . High-affinity binding of a fungal oligo peptide elicitor to parsley plasma membranes triggers multiple defense responses . Cell , 78 : 449 – 460 .
   PubMed Web of Science ®Google Scholar
• Okada , T. and Yoshizumi , H. 1973 . The mode of action of toxic protein in wheat and barley on brewing yeast . Agric. Biol. Chem. , 37 : 2289 – 2294 .
   Web of Science ®Google Scholar
• Okada , T. , Yoshizumi , H. and Terashima , Y. 1970 . A lethal toxic substance for brewing yeast in wheat and barley . Agric. Biol. Chem. , 34 : 1084 – 1088 .
   Web of Science ®Google Scholar
• Onaderra , M. , Monsalve , R. I. , Mancheno , J. M. , Villalba , M. , del Pozo , A. Marinez , Gavilanes , J. G. and Rodriguez , R. 1994 . Food mustard allergen interaction with phospholipid vesicles . Eur. J. Biochem. , 225 : 609 – 615 .
   PubMedGoogle Scholar
• Osborn , R. W. , De Samblanx , G. W. , Thevissen , K. , Goderis , I. , Torrekens , S. , Van Leuven , F. , Attenborough , S. , Rees , S. B. and Broekaert , W. F. 1995 . Isolation and characterisation of plant defensins from seeds of Asteraceae, Fabaceae, Hippocastanaceae and Saxifragaceae . FEBS Lett. , 368 : 257 – 262 .
   PubMed Web of Science ®Google Scholar
• Pena-Cortes , H. , Willmitzer , L. and Sanchez-Serrano , J. J. 1991 . Abscisic acid mediates wound induction but not developmental-specific expression of the proteinase inhibitor II gene family . Plant Cell , 3 : 963 – 972 .
   PubMed Web of Science ®Google Scholar
• Penninckx , I. A. M. A. , Eggermont , K. , Terras , F. R. G. , Thomma , B. P. H. J. , De Samblanx , G. W. , Buchala , A. , Métraux , J.-P. , Manners , J. M. and Broekaert , W. F. 1996 . Pathogen-induced systemic activation of a plant defensin gene in Arabidopsis follows a salicylic acid-independent pathway . Plant Cell , 8 : 2309 – 2323 .
   PubMed Web of Science ®Google Scholar
• Pineiro , M. , Diaz , I. , Rodriguez-Palanzuela , P. , Titarenko , E. and Garcia-Olmedo , F. 1995 . Selective disulphide linkage of plant thionins with other proteins . FEBS Lett. , 369 : 239 – 242 .
   PubMed Web of Science ®Google Scholar
• Ponz , F. , Hernandez-Lucas , C. , Carbonero , P. and Garcia-Olmedo , F. 1983 . Synthesis and processing of thionin precursors in developing endosperm from barley (Hordeum vulgare L.) . EMBO J. , 2 : 1035 – 1040 .
   PubMed Web of Science ®Google Scholar
• Ponz , F. , Paz-Ares , J. , Hernandez-Lucas , C. , Garcia-Olmedo , F. and Carbonero , P. 1986 . Cloning and nucleotide sequence of a cDNA encoding the precursor of the barley toxin α hordothionin . Eur. J. Biochem. , 156 : 131 – 135 .
   PubMedGoogle Scholar
• Pyee , J. and Kolattukudy , P. E. 1995 . The gene for the major cuticular wax-associated protein and three homologous genes from broccoli (Brassica oleracea) and their expression patterns . Plant J. , 7 : 49 – 59 .
   PubMed Web of Science ®Google Scholar
• Pyee , J. , Yu , H. and Kolattukudy , P. E. 1994 . Identification of a lipid transfer protein as the major protein in the surface wax of broccoli (Brassica oleracea) leaves . Arch. Biochem. Biophys. , 311 : 460 – 468 .
   PubMed Web of Science ®Google Scholar
• Raikhel , N. V. , Lee , H. I. and Broekaert , W. F. 1993 . Structure and function of chitin-binding proteins . Annu. Rev. Plant Physiol. Plant Mol. Biol. , 44 : 591 – 615 .
   Web of Science ®Google Scholar
• Rao , U. , Stec , B. and Teeter , M. M. 1995 . Refinement of purothionins reveals solute particles important for lattice formation and toxicity. I. α1-purothionin revisited . Acta Crystallogr. D. , 51 : 904 – 913 .
   PubMedGoogle Scholar
• Reimann-Philipp , U. , Behnke , S. , Batschauer , A. , Schafer , E. and Apel , K. 1989a . The effect of light on the biosynthesis of leaf-specific thionins in barley . Hordeum vulgare. Eur. J. Biochem. , 182 : 283 – 289 .
   PubMed Web of Science ®Google Scholar
• Reimann-Philipp , U. , Schrader , G. , Martinoia , E. , Barkholt , V. and Apel , K. 1989b . Intracellular thionins of barley. A second group of leaf thionins closely related to but distinct from cell wall-bound thionins . J. Biol. Chem. , 264 : 8978 – 8984 .
   PubMed Web of Science ®Google Scholar
• Rice , W. G. , Ganz , T. , Kinkade , J. M. , Selsted , M. E. , Lehrer , R. I. and Parmley , R. T. 1987 . Defensin-rich dense granules of human neutrophils . Blood , 70 : 757 – 765 .
   PubMed Web of Science ®Google Scholar
• Rosell , S. and Samuelsson , G. 1966 . Effect of mistletoe viscotoxin and phoratoxin on blood circulation . Toxicon. , 4 : 107 – 110 .
   PubMed Web of Science ®Google Scholar
• Samuelsson , G. 1974 . Mistletoe toxins . Syst. Zool. , 22 : 566 – 569 .
   Google Scholar
• Schrader-Fischer , G. and Apel , K. 1993 . cDNA-derived identification of novel thionin precursors in Viscum album that contain highly divergent thionin domains but conserved signal and acidic polypeptide domains . Plant Mol. Biol. , 23 : 1233 – 1242 .
   PubMed Web of Science ®Google Scholar
• Schrader-Fischer , G. and Apel , K. 1994 . Organ-specific expression of highly divergent thionin variants that are distinct from the seed-specific crambin in the crucifer Crambe abyssinica . Mol. Gen. Genet. , 245 : 380 – 389 .
   PubMedGoogle Scholar
• Segura , A. , Moreno , M. and Garcia-Olmedo , F. 1993 . Purification and antipathogenic activity of lipid transfer proteins (LTPs) from the leaves of Arabidopsis and spinach . FEBS Lett. , 332 : 243 – 246 .
   PubMed Web of Science ®Google Scholar
• Selsted , M. E. , Miller , S. I. , Henschen , A. H. and Ouelette , A. J. 1992 . Enteric defensins: antibiotic peptide components of intestinal host defense . J. Cell Biol. , 118 : 929 – 936 .
   PubMed Web of Science ®Google Scholar
• Shade , R. E. , Schroeder , H. E. , Pueyo , J. J. , Tabe , L. M. , Murdock , L. L. , Higgins , T. J. V. and Chrispeels , M. J. 1994 . Transgenic pea seeds expressing the α-amylase inhibitor of the common bean are resistant to bruchid beetles . Biotechnology , 12 : 793 – 796 .
   Google Scholar
• Shin , D. H. , Lee , J. Y. , Hwang , K. Y. , Kim , K. K. and Suh , S. W. 1995 . High-resolution crystal structure of the non-specific lipid-transfer protein from maize seedlings . Structure , 3 : 189 – 199 .
   PubMed Web of Science ®Google Scholar
• Showalter , A. M. 1993 . Structure and function of plant cell wall proteins . Plant Cell , 5 : 9 – 23 .
   PubMed Web of Science ®Google Scholar
• Simorre , J. P. , Caille , A. , Marion , D. and Ptak , M. 1991 . Two- and three-dimensional 1H NMR studies of a wheat phospholipid transfer protein: sequential resonance assignments and secondary structure . Biochemistry , 30 : 11600 – 11608 .
   PubMed Web of Science ®Google Scholar
• Sossountzov , L. , Ruiz-Avila , L. , Vignols , F. , Jolliot , A. , Arondel , V. , Tchang , F. , Grosbois , M. , Guerbette , F. , Miginiac , E. , Delseny , M. , Puigdomenech , P. and Kader , J. C. 1991 . Spatial and temporal expression of a maize lipid transfer protein gene . Plant Cell , 3 : 923 – 933 .
   PubMed Web of Science ®Google Scholar
• Stec , B. , Rao , U. and Teeter , M. M. 1995 . Refinement of purothionis reveals solute particles important for lattice formation and toxicity. II. structure of β-purothionin at 1.7 Å resolution . Acta Crystallogr. D. , 51 : 914 – 924 .
   PubMedGoogle Scholar
• Steinmüller , K. , Batschauer , A. and Apel , K. 1986 . Tissue-specific and light-dependent changes of chromatin organization in barley (Hordeum vulgare) . Eur. J. Biochem. , 158 : 519 – 525 .
   PubMedGoogle Scholar
• Sterk , P. , Booij , H. , Schellekens , G. A. , Van Kammen , A. and De Vries , S. C. 1991 . Cell-specific expression of the carrot EP2 lipid transfer protein gene . Plant Cell , 3 : 907 – 921 .
   PubMed Web of Science ®Google Scholar
• Stiekema , W. J. , Heidekamp , F. , Dirkse , W. G. , Van Beckum , J. , De Haan , P. , Ten Bosch , C. and Louwerse , J. D. 1988 . Molecular cloning and analysis of four potato tuber mRNAs . Plant Mol. Biol. , 11 : 255 – 269 .
   PubMed Web of Science ®Google Scholar
• Stuart , L. S. and Harris , T. H. 1942 . Bactericidal and fungicidal properties of a crystalline protein from unbleached wheat flour . Cereal Chem. , 19 : 288 – 300 .
   Google Scholar
• Tailor , R. , Acland , D. P. , Attenborough , S. , Cammue , B. P. A. , Evans , I. J. , Osborn , R. W. , Ray , J. , Rees , S. B. and Broekaert , W. F. 1997 . A novel family of small cysteine-rich antimicrobial peptides from seed of Impatiens balsamina is derived from a single precursor protein (submitted for publication to J. Biol. Chem.)
   Google Scholar
• Terras , F. R. G. , Eggermont , K. , Kovaleva , V. , Raikhel , N. V. , Osborn , R. W. , Kester , A. , Rees , S. B. , Vanderleyden , J. , Cammue , B. P. A. and Broekaert , W. F. 1995 . Small cysteine-rich antifungal proteins from radish: their role in host defence . Plant Cell , 7 : 573 – 588 .
   PubMed Web of Science ®Google Scholar
• Terras , F. R. G. , Goderis , I. J. , Van Leuven , F. , Vanderleyden , J. , Cammue , B. P. A. and Broekaert , W. F. 1992a . In vitro anti-fungal activity of a radish (Raphanus sativus L.) seed protein homologous to nonspecific lipid transfer proteins . Plant Physiol. , 100 : 1055 – 1058 .
   PubMed Web of Science ®Google Scholar
• Terras , F. R. G. , Schoofs , H. , De Bolle , M. F. C. , Van Leuven , F. , Rees , S. B. , Vanderleyden , J. , Cammue , B. P. A. and Broekaert , W. F. 1992b . Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds . J. Biol. Chem. , 267 : 15301 – 15309 .
   PubMed Web of Science ®Google Scholar
• Terras , F. R. G. , Schoofs , H. M. E. , Thevissen , K. , Osborn , R. W. , Vanderleyden , J. , Cammue , B. P. A. and Broekaert , W. F. 1993a . Synergistic enhancement of the antifungal activity of wheat and barley thionins by radish and oilseed rape 2S albumins and by barley trypsin inhibitors . Plant Physiol. , 103 : 1311 – 1319 .
   PubMed Web of Science ®Google Scholar
• Terras , F. R. G. , Torrekens , S. , Van Leuven , F. and Broekaert , W. F. 1996 . A six-cysteine type thionin from the radish storage organ displays weak in vitro antifungal activity against Fusarium culmorum . Plant Physiol. Biochem. , 34 : 599 – 603 .
   Web of Science ®Google Scholar
• Terras , F. R. G. , Torrekens , S. , Van Leuven , F. , Osborn , R. W. , Vanderleyden , J. , Cammue , B. P. A. and Broekaert , W. F. 1993b . A new family of basic cysteine-rich antifungal proteins from Brassicaceae species . FEBS Lett. , 316 : 233 – 240 .
   PubMed Web of Science ®Google Scholar
• Thevissen , K. , Ghazi , A. , De Samblanx , G. W. , Brownlee , C. , Osborn , R. W. and Broekaert , W. F. 1996 . Fungal membrane responses induced by plant defensins and thionins . J. Biol. Chem. , 271 : 15018 – 15025 .
   PubMed Web of Science ®Google Scholar
• Thoma , S. , Hecht , U. , Kippers , A. , Botella , J. , De Vries , S. and Somerville , C. 1994 . Tissue-specific expression of a gene encoding a cell wall-localized lipid transfer protein from Arabidopsis . Plant Physiol. , 105 : 35 – 45 .
   PubMed Web of Science ®Google Scholar
• Thoma , S. , Kaneko , Y. and Somerville , C. 1993 . A non-specific lipid transfer protein from Arabidopsis is a cell wall protein . Plant J. , 3 : 427 – 436 .
   PubMed Web of Science ®Google Scholar
• Torres-Schumann , S. , Godoy , J. A. and Pintor-Toro , J. A. 1992 . A probable lipid transfer protein gene is induced by NaCl in stems of tomato plants . Plant Mol Biol. , 18 : 749 – 757 .
   PubMed Web of Science ®Google Scholar
• Van Parijs , J. , Broekaert , W. F. , Goldstein , I. J. and Peumans , W. J. 1991 . Hevein: an antifungal protein from rubber tree (Hevea brasiliensis) latex . Planta , 183 : 258 – 264 .
   PubMed Web of Science ®Google Scholar
• Vaz Gomes , A. , de Waal , A. , Berden , W. J. A. and Westerhoff , H. V. 1993 . Electric potentation, cooperativity and synergism of magainin peptides in protein-free liposomes . Biochemistry , 32 : 5365 – 5372 .
   PubMed Web of Science ®Google Scholar
• Vernon , L. P. 1992 . Pyrularia thionin: physical properties, biological responses and comparison to other thionins and cardiotoxin . J. Toxicol. , 11 : 169 – 191 .
   Web of Science ®Google Scholar
• Vernon , L. P. , Evett , G. E. , Zeikus , R. D. and Gray , W. R. 1985 . A toxic thionin from Pyrularia pubera: purification, properties, and amino acid sequence . Arch. Biochem. Biophys. , 238 : 18 – 29 .
   PubMed Web of Science ®Google Scholar
• Wall , J. , Golding , C. A. , Van Veen , M. and O'Shea , P. 1995 . The use of fluoresceinphosphatidyl-ethanolamine (FPE) as a real-time probe for peptide-membrane interactions . Mol. Membrane Biol. , 12 : 183 – 192 .
   PubMed Web of Science ®Google Scholar
• Walujono , K. , Scholma , R. A. , Beintema , J. J. , Mariono , A. and Hahn , A. M. 1975 . Amino acid sequence of hevein . Proc. Int. Rubber Conf., Kuala Lumpur , 2 : 518 – 531 .
   Google Scholar
• Zeevaart , J. A. D. 1988 . Metabolism and physiology of abscisic acid . Annu. Rev. Plant Physiol. Plant Mol. Biol. , 39 : 439 – 473 .
   Web of Science ®Google Scholar