An overview of Bacillus proteases: from production to application

References

• Garcia-Carreno FL, Navarrete MA, Toro DEL. Classification of proteases without tears. Biochem Educ. 1997;25:161–167.
   Google Scholar
• Grassmann W, Dyckerhoff H. Über die Proteinase und die polypeptidase der hefe. Hoppe-Seyler’s Z Physiol Chem. 1928;179:41–78.
   Google Scholar
• Barrett AJ, McDonald JK. Nomenclature: protease, proteinase and peptidase. Biochem J. 1986;237:935.
   PubMed Web of Science ®Google Scholar
• Rawlings ND, Barrett AJ, Bateman A. MEROPS: the peptidase database. Nucleic Acids Res. 2010;38:D227–D233.
   PubMed Web of Science ®Google Scholar
• Rawlings ND, Tole DP, Barrett AJ. MEROPS: the peptidase database. Nucleic Acids Res. 2004;32:D160–D164.
   PubMed Web of Science ®Google Scholar
• Kuddus M, Singh P, Thomas G, et al. Recent developments in production and biotechnological applications of c-phycocyanin. Biomed Res Int. 2013;2013:330–338.
   Google Scholar
• Ahmed Z, Donkor O, Street WA, et al. Proteolytic activities in fillets of selected underutilized Australian fish species. Food Chem. 2013;140:238–244.
   PubMed Web of Science ®Google Scholar
• Sharma KM, Kumar R, Panwar S, et al. Microbial alkaline proteases: optimization of production parameters and their properties. J Genet Eng Biotechnol. 2017;15:115–126.
   PubMedGoogle Scholar
• Tufvesson P, Lima-Ramos J, Nordblad M, et al. Guidelines and cost analysis for catalyst production in biocatalytic processes. Org Process Res Dev. 2011;15:266–274.
   Web of Science ®Google Scholar
• Belmessikh A, Boukhalfa H, Mechakra-Maza A, et al. Statistical optimization of culture medium for neutral protease production by Aspergillus oryzae. Comparative study between solid and submerged fermentations on tomato pomace. J Taiwan Inst Chem Eng. 2013;44:377–385.
   Web of Science ®Google Scholar
• Chi Z, Ma C, Wang P, et al. Optimization of medium and cultivation conditions for alkaline protease production by the marine yeast Aureobasidium pullulans. Bioresour Technol. 2007;98:534–538.
   PubMed Web of Science ®Google Scholar
• Bach E, Sant’Anna V, Daroit DJ, et al. Production, one-step purification, and characterization of a keratinolytic protease from Serratia marcescens P3. Process Biochem. 2012;47:2455–2462.
   Web of Science ®Google Scholar
• Auther C, Helal M, Amer H, et al. Physiological and microbiological studies on production of alkaline protease from locally isolated Bacillus subtilis. Aust J Basic Appl Sci. 2012;6:193–203.
   Google Scholar
• Harwood CR, Cranenburgh R. Bacillus protein secretion: an unfolding story. Trends Microbiol. 2008;16:73–79.
   PubMed Web of Science ®Google Scholar
• Rehman R, Ahmed M, Siddique A, et al. Catalytic role of thermostable metalloproteases from Bacillus subtilis KT004404 as dehairing and destaining agent. Appl Biochem Biotechnol. 2017;181:434–450.
   PubMed Web of Science ®Google Scholar
• Anandharaj M, Sivasankari B, Siddharthan N, et al. Production, purification, and biochemical characterization of thermostable metallo-protease from novel Bacillus alkalitelluris TWI3 isolated from tannery waste. Appl Biochem Biotechnol. 2016;178:1666–1686.
   PubMed Web of Science ®Google Scholar
• Vetter R, Wilke D, Möller B, et al. Alkalische proteasen aus Bacillus pumilus. EP 0572992 A1; 1993.
   Google Scholar
• Merkel M, Siegert P, Wieland S, et al. Subtilisin from Bacillus pumilus and washing and cleaning agents containing said novel subtilisin. WO/2007/131656; 2007.
   Google Scholar
• Shikha SA, Darmwal NS. Improved production of alkaline protease from a mutant of alkalophilic Bacillus pantotheneticus using molasses as a substrate. Bioresour Technol. 2007;98:881–885.
   PubMed Web of Science ®Google Scholar
• Sangeetha R, Geetha A, Arulpandi I. Pongamia pinnata seed cake: a promising and inexpensive substrate for production of protease and lipase from Bacillus pumilus SG2 on solid-state fermentation. Indian J Biochem Biophys. 2011;48:435–439.
   PubMed Web of Science ®Google Scholar
• Shivasharana CT, Naik GR. Production of alkaline protease from a thermoalkalophilic Bacillus sp. JB-99 under solid state fermentation. Int J Pharm Biol Sci. 2012;3:571–587.
   Google Scholar
• Jaouadi NZ, Jaouadi B, Aghajari N, et al. The overexpression of the SAPB of Bacillus pumilus CBS and mutated sapB-L31I/T33S/N99Y alkaline proteases in Bacillus subtilis DB430: new attractive properties for the mutant enzyme. Bioresour Technol. 2012;105:142–151.
   PubMed Web of Science ®Google Scholar
• Annamalai N, Rajeswari MV, Thavasi R, et al. Optimization, purification and characterization of novel thermostable, haloalkaline, solvent stable protease from Bacillus halodurans CAS6 using marine shellfish wastes: a potential additive for detergent and antioxidant synthesis. Bioprocess Biosyst Eng. 2013;36:873–883.
   PubMed Web of Science ®Google Scholar
• Bougatef A, Balti R, Haddar A, et al. Protein hydrolysates from bluefin tuna (Thunnus thynnus) heads as influenced by the extent of enzymatic hydrolysis. Biotechnol Bioproc E. 2012;17:841–852.
   Web of Science ®Google Scholar
• Ozcan T, Kurdal E. The effects of using a starter culture, lipase, and protease enzymes on ripening of Mihalic cheese. Int J Dairy Technol. 2012;65:585–593.
   Web of Science ®Google Scholar
• Caille J, Govindan CK, Junga H. Hetero diels-alder-biocatalysis approach for the synthesis of (s) -3-[2-{(methylsulfonyl)oxy}ethoxy]-4-(triphenylmethoxy)-1-butanol methanesulfonate, a key intermediate for the synthesis of the pkc inhibitor. Org Process Res Dev. 2002;6:471–476.
   Web of Science ®Google Scholar
• Ferreira L, Ramos MA, Dordick JS, et al. Influence of different silica derivatives in the immobilization and stabilization of a Bacillus licheniformis protease (Subtilisin Carlsberg). J Mol Catal B Enzym. 2003;21:189–199.
   Google Scholar
• Gopal N, Hill C, Ross PR, et al. The prevalence and control of Bacillus and related spore-forming bacteria in the dairy industry. Front Microbiol. 2015;6:1418.
   PubMed Web of Science ®Google Scholar
• Alcaraz LD, Olmedo G, Bonilla G, et al. The genome of Bacillus coahuilensis reveals adaptations essential for survival in the relic of an ancient marine environment. Proc Natl Acad Sci USA. 2008;105:5803–5808.
   PubMed Web of Science ®Google Scholar
• Gupta R, Beg Q, Lorenz P. Bacterial alkaline proteases: Molecular approaches and industrial applications. Appl Microbiol Biotechnol. 2002;59:15–32.
   PubMed Web of Science ®Google Scholar
• Sumantha A, Larroch C, Pandey A. Microbiology and industrial biotechnology of food-grade proteases: a perspective. Food Technol Biotechnol. 2006;44:211–220.
   Web of Science ®Google Scholar
• Sewalt V, Shanahan D, Gregg L, et al. The Generally Recognized as Safe (GRAS) process for industrial microbial enzymes. Indus Biotechnol. 2016;12:295–302.
   Google Scholar
• Schallmey M, Singh A, Ward OP. Developments in the use of Bacillus species for industrial production. Can J Microbiol. 2004;50:1–17.
   PubMed Web of Science ®Google Scholar
• Rahmer R, Heravi KM, Altenbuchner J. Construction of a super-competent Bacillus subtilis 168 using the PmtlA-comKS inducible cassette. Front Microbiol. 2015;6:1431.
   PubMed Web of Science ®Google Scholar
• Adinarayana K, Ellaiah P, Prasad DS. Purification and partial characterization of thermostable serine alkaline protease from a newly isolated Bacillus subtilis PE-11. AAPS PharmSciTech. 2003;4:E56.
   PubMedGoogle Scholar
• Rozs M, Manczinger L, Vágvölgyi C, et al. Secretion of a trypsin-like thiol protease by a new keratinolytic strain of Bacillus licheniformis. FEMS Microbiol Lett. 2001;205:221–224.
   PubMed Web of Science ®Google Scholar
• Sookkheo B, Sinchaikul S, Phutrakul S, et al. Purification and characterization of the highly thermostable proteases from Bacillus stearothermophilus TLS33. Protein Expr Purif. 2000;20:142–151.
   PubMed Web of Science ®Google Scholar
• Briki S, Hamdi O, Landoulsi A. Enzymatic dehairing of goat skins using alkaline protease from Bacillus sp. SB12. Protein Expr Purif. 2016;121:9–16.
   PubMed Web of Science ®Google Scholar
• Sathishkumar R, Ananthan G, Arun J. Production, purification and characterization of alkaline protease by ascidian associated Bacillus subtilis GA CAS8 using agricultural wastes. Biocatal Agric Biotechnol. 2015;4:214–220.
   Web of Science ®Google Scholar
• Farhadian S, Asoodeh A, Lagzian M. Purification, biochemical characterization and structural modeling of a potential htrA-like serine protease from Bacillus subtilis DR8806. J Mol Catal B. 2015;115:51–58.
   Google Scholar
• Annamalai N, Rajeswari MV, Balasubramanian T. Extraction, purification and application of thermostable andhalostable alkaline protease from Bacillus alveayuensis CAS 5using marine wastes. Food Bioprod Process. 2014;92:335–342.
   Web of Science ®Google Scholar
• Wang J, Xu A, Wan Y, et al. Purification and characterization of a new metallo-neutral protease for beer brewing from Bacillus amyloliquefaciens SYB-001. Appl Biochem Biotechnol. 2013;170:2021–2033.
   PubMed Web of Science ®Google Scholar
• Anbu P. Characterization of solvent stable extracellular protease from Bacillus koreensis (BK-P21A). Int J Biol Macromol. 2013;56:162–168.
   PubMed Web of Science ®Google Scholar
• Jain D, Pancha I, Mishra SK, et al. Purification and characterization of haloalkaline thermoactive, solvent stable and SDS-induced protease from Bacillus sp.: a potential additive for laundry detergents. Bioresour Technol. 2012;115:228–236.
   PubMed Web of Science ®Google Scholar
• Rajkumar R, Jayappriyan KR, Rengasamy R. Purification and characterization of a protease produced by Bacillus megaterium RRM2: application in detergent and dehairing industries. J Basic Microbiol. 2011;51:614–624.
   PubMed Web of Science ®Google Scholar
• Singh SK, Singh SK, Tripathi VR, et al. Purification, characterization and secondary structure elucidation of a detergent stable, halotolerant, thermoalkaline protease from Bacillus cereus SIU1. Process Biochem. 2012;47:1479–1487.
   Web of Science ®Google Scholar
• Duman RE, Löwe J. Crystal structures of Bacillus subtilis lon protease. J Mol Biol. 2010;401:653–670.
   PubMed Web of Science ®Google Scholar
• Betzel C, Klupsch S, Branner S, et al. Crystal structures of the alkaline proteases savinase and esperase from Bacillus lentus. Adv Exp Med Biol. 1996;379:49–61.
   PubMedGoogle Scholar
• Nonaka T, Fujihashi M, Kita A, et al. The crystal structure of an oxidatively stable subtilisin-like alkaline serine protease, KP-43, with a C-terminal beta-barrel domain. J Biol Chem. 2004;279:47344–47345.
   PubMed Web of Science ®Google Scholar
• Siezen RJ, Leunissen JA. Subtilases: the superfamily of subtilisin-like serine proteases. Protein Sci. 1997;6:501–523.
   PubMed Web of Science ®Google Scholar
• Betzel C, Klupsch S, Papendorf G, et al. Crystal structure of the alkaline proteinase Savinase from Bacillus lentus at 1.4 A resolution. J Mol Biol. 1992;223:427–445.
   PubMed Web of Science ®Google Scholar
• Yamane T, Kani T, Hatanaka T, et al. Structure of a new alkaline serine protease (M-protease) from Bacillus sp. KSM-K16. Acta Crystallogr D Biol Crystallogr. 1995;51:199–206.
   PubMedGoogle Scholar
• Shirai T, Suzuki A, Yamane T, et al. High-resolution crystal structure of M-protease: Phylogeny aided analysis of the high-alkaline adaptation mechanism. Protein Eng. 1997;10:627–634.
   PubMedGoogle Scholar
• Lee BG, Kim MK, Song HK. Structural insights into the conformational diversity of ClpP from Bacillus subtilis. Mol Cells. 2011;32:589–595.
   PubMed Web of Science ®Google Scholar
• Nam SE, Kim AC, Paetzel M. Crystal structure of Bacillus subtilis signal peptide peptidase A. J Mol Biol. 2012;419:347–358.
   PubMed Web of Science ®Google Scholar
• Rao MB, Tanksale AM, Ghatge MS, et al. Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev. 1998;62:597–635.
   PubMed Web of Science ®Google Scholar
• Fastrez J, Fersht AR. Demonstration of the acyl-enzyme mechanism for the hydrolysis of peptides and anilides by chymotrypsin. Biochemistry. 1973;12:2025–2034.
   PubMed Web of Science ®Google Scholar
• Roderick SL, Matthews BW. Structure of the cobalt-dependent methionine aminopeptidase from Escherichia coli: A new type of proteolytic enzyme. Biochemistry. 1993;32:3907–3912.
   PubMed Web of Science ®Google Scholar
• Cottrell GS, Hooper NM, Turner AJ. Cloning, expression, and characterization of human cytosolic aminopeptidase P: A single manganese(II)-dependent enzyme. Biochemistry. 2000;39:15121–15128.
   PubMed Web of Science ®Google Scholar
• Heitzer M, Hallmann A. An extracellular matrix-localized metalloproteinase with an exceptional QEXXH metal binding site prefers copper for catalytic activity. J Biol Chem. 2002;277:28280–28286.
   PubMed Web of Science ®Google Scholar
• Kirk O, Borchert TV, Fuglsang CC. Industrial enzyme applications. Curr Opin Biotechnol. 2002;13:345–351.
   PubMed Web of Science ®Google Scholar
• Baweja M, Tiwari R, Singh PK, et al. An alkaline protease from Bacillus pumilus MP 27: functional analysis of its binding model toward its applications as detergent additive. Front Microbiol. 2016;7:1–14.
   PubMed Web of Science ®Google Scholar
• Oliveira CTD, Rieger TJ, Daroit DJ. Catalytic properties and thermal stability of a crude protease from the keratinolytic Bacillus sp. CL33A. Biocatal Agric Biotechnol. 2017;10:270–277.
   Google Scholar
• Kim M, Si J-B, Reddy LV, et al. Enhanced production of extracellular proteolytic enzyme excreted by a newly isolated Bacillus subtilis FBL-1 through combined utilization of statistical designs and response surface methodology. RSC Adv. 2016;6:51270–51278.
   Web of Science ®Google Scholar
• Moorthy I, Baskar R. Statistical modeling and optimization of alkaline protease production from a newly isolated alkalophilic Bacillus species BGS using response surface methodology and genetic algorithm. Prep Biochem Biotechnol. 2013;43:293–314.
   PubMed Web of Science ®Google Scholar
• Nadeem M, Baig S, Syed QUA, et al. Microbial production of alkaline proteases by locally isolated Bacillus subtilis PCSIR-5. Pak J Zool. 2006;38:109–114.
   Google Scholar
• Qureshi AS, Khushk I, Ali CH, et al. Coproduction of protease and amylase by thermophilic Bacillus sp. BBXS-2 using open solid-state fermentation of lignocellulosic biomass. Biocatal Agric Biotechnol. 2016;8:146–151.
   Google Scholar
• Vijayaraghavan P, Lazarus S, Vincent SGP. De-hairing protease production by an isolated Bacillus cereus strain AT under solid-state fermentation using cow dung: Biosynthesis and properties. Saudi J Biol Sci. 2014;21:27–34.
   PubMed Web of Science ®Google Scholar
• Rathakrishnan P, Nagarajan P. Red gram husk: a potent substrate for production of protease by Bacillus cereus in solid-state fermentation. Int J ChemTech Res. 2011;3:1526–1533.
   Google Scholar
• Gupta R, Beg QK, Khan S, et al. An overview on fermentation, downstream processing and properties of microbial alkaline proteases. Appl Microbiol Biotechnol. 2003;60:381–395.
   Web of Science ®Google Scholar
• Pandey A. Recent process developments in solid-state fermentation. Process Biochem. 1992;27:109–117.
   Web of Science ®Google Scholar
• Couto RS, Sanromán M. Effect of two wastes fromgroundnut processing on laccase production and dye decolouriza-tion ability. J Food Eng. 2006;73:388–393.
   Web of Science ®Google Scholar
• Pandey A, Soccol CR, Nigam P, et al. Biotechnological potential of agro-industrial residues. I: Sugarcane bagasse. Bioresour Technol. 2000;74:69–80.
   Web of Science ®Google Scholar
• Lonsane BK, Ghildyal NP, Budiatman S, et al. Engineering aspects of solid state fermentation. Enzyme Microb Technol. 1985;7:258–265.
   Web of Science ®Google Scholar
• Chiliveri SR, Koti S, Linga VR. Retting and degumming of natural fibers by pectinolytic enzymes produced from Bacillus tequilensis SV11-UV37 using solid state fermentation. Springerplus. 2016;5:559.
   PubMed Web of Science ®Google Scholar
• Pandey A, Soccol CR, Mitchell D. New developments in solid state fermentation: I-bioprocesses and products. Process Biochem. 2000;35:1153–1169.
   Web of Science ®Google Scholar
• O’Hara MB, Hageman JH. Energy and calcium ion dependence of proteolysis during sporulation of Bacillus subtilis cells. J Bacteriol. 1990;172:4161–4170.
   PubMed Web of Science ®Google Scholar
• Hanlon GW, Hodges NA. Bacitracin and protease production in relation to sporulation during exponential growth of Bacillus licheniformis on poorly utilized carbon and nitrogen sources. J Bacteriol. 1981;147:427–431.
   PubMed Web of Science ®Google Scholar
• Joo HS, Chang CS. Production of an oxidant and SDS-stable alkaline protease from an alkaophilic Bacillus clausii I-52 by submerged fermentation: feasibility as a laundry detergent additive. Enzyme Microb Technol. 2006;38:176–183.
   Web of Science ®Google Scholar
• Huang R, Yang Q, Feng H. Single amino acid mutation alters thermostability of the alkaline protease from Bacillus pumilus: thermodynamics and temperature dependence. Acta Biochim Biophys Sin. 2015;47:98–105.
   Google Scholar
• Jaouadi B, Aghajari N, Haser R, et al. Enhancement of the thermostability and the catalytic efficiency of Bacillus pumilus CBS protease by site-directed mutagenesis. Biochimie. 2010;92:360–369.
   PubMed Web of Science ®Google Scholar
• Jaouadi NZ, Jaouadi B, Hlima HB, et al. Probing the crucial role of Leu31 and Thr33 of the Bacillus pumilus CBS alkaline protease in substrate recognition and enzymatic depilation of animal hide. PLoS One. 2014;9:e108367.
   PubMed Web of Science ®Google Scholar
• Liu Y, Zhang T, Zhang Z, et al. Enzymatic improvement of cold adaptation of Bacillus alcalophilus alkaline protease by directed evolution. J Mol Catal B. 2014;106:117–123.
   Google Scholar
• Van den Burg B, de Kreij A, Van der Veek P, et al. Characterization of a novel stable biocatalyst obtained by protein engineering. Biotechnol Appl Biochem. 1999;30:35–40.
   PubMed Web of Science ®Google Scholar
• Martinez R, Jakob F, Tu R, et al. Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution. Biotechnol Bioeng. 2013;110:711–720.
   PubMed Web of Science ®Google Scholar
• Wang H, Yang L, Ping Y, et al. Engineering of a Bacillus amyloliquefaciens strain with high neutral protease producing capacity and optimization of its fermentation conditions. PLoS One. 2016;11:e0146373.
   PubMed Web of Science ®Google Scholar
• Diep TB, Thom NT, Sang HD, et al. Screening streptomycin resistant mutations from gamma ray irradiated Bacillus subtilis B5 for selection of potential mutants with high production of protease. J Sci Nat Sci Technol. 2016;32:170–176.
   Google Scholar
• Feldman LI. Preparation of microbial alkaline protease by fermentation with Bacillus subtilis, variety licheniformis. US3623957 A; 1971.
   Google Scholar
• Rahman RNZ, Salleh A, Basri M, et al. Production of protease from Bacillus stearothermophilus F1. US20050186661 A1; 2005.
   Google Scholar
• Shih J. Construction of Bacillus licheniformis T1 strain, and fermentation production of crude enzyme extract therefrom. US20050032188 A17; 2005.
   Google Scholar
• Weber A, Hellebrandt A, Wieland S, et al. Alkaline protease from Bacillus gibsonii (DSM 14393) and washing and cleaning products comprising said alkaline protease. US7262042 B2; 2007.
   Google Scholar
• Quaedflieg PJLM, Nuijens T. Enzymatic synthesis of amino acid and peptide c-terminal amides. WO2013129927 A1; 2013.
   Google Scholar
• Damodaran S, Han X-Q. Detergent-stable alkaline protease from Bacillus pumilus. US 5976859 A; 1996.
   Google Scholar
• Adrio JL, Demain AL. Microbial enzymes: tools for biotechnological processes. Biomolecules. 2014;4:117–139.
   PubMedGoogle Scholar
• Hmidet N, El-Hadj AN, Haddar A, et al. Alkaline proteases and thermostable α-amylase co-produced by Bacillus licheniformis NH1: characterization and potential application as detergent additive. Biochem Eng J. 2009;47:71–79.
   Web of Science ®Google Scholar
• Lagzian M, Asoodeh A. An extremely thermotolerant, alkaliphilic subtilisin-like protease from hyperthermophilic Bacillus sp. MLA64. Int J Biol Macromol. 2012;51:960–967.
   PubMed Web of Science ®Google Scholar
• Joshi S, Satyanarayana T. Characteristics and applications of a recombinant alkaline serine protease from a novel bacterium Bacillus lehensis. Bioresour Technol. 2013;131:76–85.
   PubMed Web of Science ®Google Scholar
• Sinha R, Khare SK. Characterization of detergent compatible protease of a halophilic Bacillus sp. EMB9: Differential role of metal ions in stability and activity. Bioresour Technol. 2013;145:357–361.
   PubMed Web of Science ®Google Scholar
• Danquah MK, Agyei D. Pharmaceutical applications of bioactive peptides. OA Biotechnol. 2012;1:5.
   Google Scholar
• Korhonen H, Pihlanto A. Bioactive peptides: production and functionality. Int Dairy J. 2006;16:945–960.
   Web of Science ®Google Scholar
• Daroit DJ, Corrěa APF, Canales MM, et al. Physicochemical properties and biological activities of ovine caseinate hydrolysates. Dairy Sci Technol. 2012;92:335–351.
   Web of Science ®Google Scholar
• Law B, Wigmore A. Microbial proteinases as agents for accelerated cheese ripening. Int J Dairy Tech. 1982;35:75–76.
   Google Scholar
• Fox PF, Wallace JM, Morgan S, et al. Acceleration of cheese ripening. Antonie Van Leeuwenhoek. 1996;70:271–297.
   PubMed Web of Science ®Google Scholar
• Zapelena MJ, Zalacain I, De Peña MP, et al. Effect of the addition of a neutral proteinase from Bacillus subtilis (Neutrase) on nitrogen fractions and texture of Spanish fermented sausage. J Agric Food Chem. 1997;45:2798–2801.
   Web of Science ®Google Scholar
• Wang C, Guan Z, He Y. Biocatalytic domino reaction: synthesis of 2H-1-benzopyran-2-one derivatives using alkaline protease from Bacillus licheniformis. Green Chem. 2011;13:2048–2054.
   Web of Science ®Google Scholar
• Xie B-H, Guan Z, He Y. Biocatalytic knoevenagel reaction using alkaline protease from Bacillus licheniformis. Biocatal Biotransformation. 2012;30:238–244.
   Web of Science ®Google Scholar
• López-Iglesias M, Busto E, Gotor V. Use of protease from Bacillus licheniformis as promiscuous catalyst for organic synthesis: applications in C-C and C-N bond formation reactions. Adv Synth Catal. 2011;353:2345–2353.
   Web of Science ®Google Scholar
• Poppe L, Novák L. Selective biocatalysis: a synthetic approach. New York: Weinheim; 1992.
   Google Scholar
• Mahmoudian M. Biocatalytic production of chiral pharmaceutical intermediates. Biocatal Biotransformation. 2000;18:105–118.
   Web of Science ®Google Scholar
• Zheng CZ, Wang JL, Li X, et al. Regioselective synthesis of amphiphilic metoprolol-saccharide conjugates by enzymatic strategy in organic media. Process Biochem. 2011;46:123–127.
   Web of Science ®Google Scholar
• Zaraî N, Rekik H, Ben M, et al. A novel keratinase from Bacillus tequilensis strain Q7 with promising potential for the leather bating process. Int J Biol Macromol. 2015;79:952–964.
   PubMed Web of Science ®Google Scholar
• Brandelli A. Bacterial keratinases: useful enzymes for bioprocessing agroindustrial wastes and beyond. Food Bioprocess Technol. 2008;1:105–116.
   Web of Science ®Google Scholar
• Lv LX, Sim MH, Li YD, et al. Production, characterization and application of a keratinase from Chryseobacterium L99 sp. nov. Process Biochem. 2010;45:1236–1244.
   Web of Science ®Google Scholar
• Liu B, Zhang J, Li B, et al. Expression and characterization of extreme alkaline, oxidation-resistant keratinase from Bacillus licheniformis in recombinant Bacillus subtilis WB600 expression system and its application in wool fiber processing. World J Microbiol Biotechnol. 2013;29:825–832.
   PubMed Web of Science ®Google Scholar
• Infante I, Morel MA, Ubalde MC, et al. Wool-degrading Bacillus isolates: extracellular protease production for microbial processing of fabrics. World J Microbiol Biotechnol. 2010;26:1047–1052.
   Web of Science ®Google Scholar
• Cooper M, Gutterres M, Marcilio N. Environmental developments and researches in Brazilian leather sector. J Soc Leather Technol Chem. 2011;50:209–211.
   Google Scholar
• Paliwal N, Singh S, Garg S. Cation-induced thermal stability of an alkaline protease from a Bacillus sp. Bioresour Technol. 1994;50:209–211.
   Web of Science ®Google Scholar