An overview of fungal biopolymers: bioemulsifiers and biosurfactants compounds production

References

• Kora AJ, Sashidhar RB, Arunachalam J. Aqueous extract of gum olibanum (Boswellia serrata): a reductant and stabilizer for the biosynthesis of antibacterial silver nanoparticles. Process Biochem. 2012;47(10):1516–1520.
   Web of Science ®Google Scholar
• Ranganathan S, Balagangadharan K, Selvamurugan N. Chitosan and gelatin-based electrospun fibers for bone tissue engineering. Int J Biol Macromol. 2019;133:354–364.
   PubMed Web of Science ®Google Scholar
• Quintana-Quirino M, Morales-Osorio C, Vigueras Ramírez G, et al. Bacterial cellulose grows with a honeycomb geometry in a solid-state culture of Gluconacetobacter xylinus using polyurethane foam support. Process Biochem. 2019;82:1–9.
   Web of Science ®Google Scholar
• Joye IJ. Cereal biopolymers for nano- and microtechnology: a myriad of opportunities for novel (functional) food applications. Trends Food Sci Technol. 2019;83:1–11.
   Web of Science ®Google Scholar
• Chanchal A, Vohra R, Elesela S, et al. Gelatin biopolymer: a journey from micro to nano. J Pharm Res. 2014;8:1387–1397.
   Google Scholar
• Brito GF, Agrawal P, Araújo EM, et al. Biopolímeros, polímeros biodegradáveis e polímeros verdes. Rev Eletron Mat Proc. 2011;2:127–139.
   Google Scholar
• Bilal M, Iqbal MN. Naturally-derived biopolymers: potential platforms for enzyme immobilization. Int J Biol Macromol. 2019;130:462–482.
   PubMed Web of Science ®Google Scholar
• Vijayendra SVN, Shamala TR. Film forming microbial biopolymers for commercial applications — a review. Crit Rev Biotechnol. 2013;8551:1–20.
   Google Scholar
• Donot F, Fontana A, Baccou JC, et al. Microbial exopolysaccharides: main examples of synthesis, excretion, genetics and extraction. Carbohydr Polym. 2012;87(2):951–962.
   Web of Science ®Google Scholar
• Castillo NA, Valdez AL, Fariña JI. Microbial production of scleroglucan and downstream processing. Front Microbiol. 2015;6:1106–1119.
   PubMed Web of Science ®Google Scholar
• Sugumaran KR, Ponnusami V. Review on production, downstream processing and characterization of microbial pullulan. Carbohydr Polym. 2017;173:573–591.
   PubMed Web of Science ®Google Scholar
• Zou X, Cheng C, Feng J, et al. Biosynthesis of polymalic acid in fermentation: advances and prospects for industrial application. Crit Rev Biotechnol. 2019;39:1–14.
   PubMed Web of Science ®Google Scholar
• Zhang B, Guan Y, Hu P, et al. Production of bioactive metabolites by submerged fermentation of the medicinal mushroom Antrodia cinnamomea: recent advances and future development. Crit Rev Biotechnol. 2019;39:1–14.
   PubMed Web of Science ®Google Scholar
• Kourmentza C, Araujo D, Sevrin C, et al. Occurrence of non-toxic bioemulsifiers during polyhydroxyalkanoate production by Pseudomonas strains valorizing crude glycerol by-product. Bioresour Technol. 2019;281:31–40.
   PubMed Web of Science ®Google Scholar
• Kopsahelis A, Kourmentza C, Zafiri C, et al. Gate-to-gate life cycle assessment of biosurfactants and bioplasticizers production via biotechnological exploitation of fats and waste oils. J Chem Technol Biotechnol. 2018;93(10):2833–2841.
   Web of Science ®Google Scholar
• Uzoigwe C, Burgess JG, Ennis CJ, et al. Bioemulsifiers are not biosurfactants and require different screening approaches. Front Microbiol. 2015;6:1–6.
   PubMed Web of Science ®Google Scholar
• Banat IM, Franzetti A, Gandolfi I, et al. Microbial biosurfactants production, applications and future potential. Appl Microbiol Biotechnol. 2010;87(2):427–444.
   PubMed Web of Science ®Google Scholar
• Cárdenas-Reyna T, Angulo C, Guluarte C, et al. In vitro immunostimulatory potential of fungal β-glucans in pacific red snapper (Lutjanus peru) cells. Dev Comp Immunol. 2017;77:350–358.
   PubMed Web of Science ®Google Scholar
• Freitas F, Torres CAV, Reis MAM. Engineering aspects of microbial exopolysaccharide production. Bioresour Technol. 2017;245(Pt B):1674–1683.
   PubMedGoogle Scholar
• Alvarez VM, Jurelevicius D, Serrato RV, et al. Chemical characterization and potential application of exopolysaccharides produced by Ensifer adhaerens JHT2 as a bioemulsifier of edible oils. Int J Biol Macromol. 2018;114:18–25.
   PubMed Web of Science ®Google Scholar
• Pessôa MG, Vespermann KAC, Paulino BN, et al. Newly isolated microorganisms with potential application in biotechnology. Biotechnol Adv. 2019;37(2):319–339.
   PubMed Web of Science ®Google Scholar
• Cirigliano MC, Carman GM. Purification and characterization of Liposan, a bioemulsifier from Candida lipolytica. Appl Environ Microbiol. 1985;50(4):846–850.
   PubMed Web of Science ®Google Scholar
• Yildiz H, Karatas N. Microbial exopolysaccharides: resources and bioactive properties. Process Biochem. 2018;72:41–46.
   Web of Science ®Google Scholar
• Kumar CG, Mongolla P, Pombala S. Lasiosan, a new exopolysaccharide from Lasiodiplodia sp. strain B2 (MTCC 6000): structural characterization and biological evaluation. Process Biochem. 2018;72:162–169.
   Web of Science ®Google Scholar
• Barreto MCS, Figueiredo MVB, Burity HA, et al. Produção e comportamento reológico de biopolímeros produzidos por rizóbios e caracterização genética. Rev Bras Agrociência. 2011;17:221–227.
   Google Scholar
• Zhao H, Li J, Zhang J, et al. Purification, in vitro antioxidant and in vivo anti-aging activities of exopolysaccharides by Agrocybe cylindracea. Int J Biol Macromol. 2017;102:351–357.
   PubMed Web of Science ®Google Scholar
• Li H, Gao T, Wang J, et al. Structural identification and antitumor activity of the extracellular polysaccharide from Aspergillus terreus. Process Biochem. 2016;51(10):1714–1720.
   Web of Science ®Google Scholar
• Wang C, Mao W, Chen Z, et al. Purification, structural characterization and antioxidant property of an extracellular polysaccharide from Aspergillus terreus. Process Biochem. 2013;48(9):1395–1401.
   Web of Science ®Google Scholar
• Orlandelli RC, Corradi da Silva ML, Vasconcelos AFD, et al. Pamphile, β-(1→3,1→6)-d-glucans produced by Diaporthe sp. endophytes: purification, chemical characterization and antiproliferative activity against MCF-7 and HepG2-C3A cells. Int J Biol Macromol. 2017;94(Pt A):431–437.
   PubMedGoogle Scholar
• Prathyusha AMVN, Mohana Sheela G, Bramhachari PV. Chemical characterization and antioxidant properties of exopolysaccharides from mangrove filamentous fungi Fusarium equiseti ANP2. Biotechnol Rep (Amst). 2018;19:e00277.
   PubMedGoogle Scholar
• He Y, Ye M, Du Z, et al. Purification, characterization and promoting effect on wound healing of an exopolysaccharide from Lachnum YM405. Carbohydr Polym. 2014;105:169–176.
   PubMed Web of Science ®Google Scholar
• Wang Y, Hou G, Li J, et al. Structure characterization, modification through carboxymethylation and sulfation, and in vitro antioxidant and hypoglycemic activities of a polysaccharide from Lachnum sp. Process Biochem. 2018;72:177–187.
   Web of Science ®Google Scholar
• Tian Y, Zhao Y, Zeng H, et al. Structural characterization of a novel neutral polysaccharide from Lentinus giganteus and its antitumor activity through inducing apoptosis. Carbohydr Polym. 2016;154:231–240.
   PubMed Web of Science ®Google Scholar
• Sun K, Chen Y, Niu Q, et al. An exopolysaccharide isolated from a coral-associated fungus and its sulfated derivative activates macrophages. Int J Biol Macromol. 2016;82:387–394.
   PubMed Web of Science ®Google Scholar
• Song X, Liu Z, Zhang J, et al. Antioxidative and hepatoprotective effects of enzymatic and acidic-hydrolysis of Pleurotus geesteranus mycelium polysaccharides on alcoholic liver diseases. Carbohydr Polym. 2018;201:75–86.
   PubMed Web of Science ®Google Scholar
• Wang KF, Sui KY, Guo C, et al. Quorum sensing molecule-farnesol increased the production and biological activities of extracellular polysaccharide from Trametes versicolor. Int J Biol Macromol. 2017;104(Pt A):377–383.
   PubMedGoogle Scholar
• Hao L, Sheng Z, Lu J, et al. Characterization and antioxidant activities of extracellular and intracellular polysaccharides from Fomitopsis pinicola. Carbohydr Polym. 2016;141:54–59.
   PubMed Web of Science ®Google Scholar
• Li K, Yu M, Hu Y, et al. Three kinds of Ganoderma lucidum polysaccharides attenuate DDC-induced chronic pancreatitis in mice. Chem Biol Interact. 2016;247:30–38.
   PubMed Web of Science ®Google Scholar
• Zhang C, Li S, Zhang J, et al. Antioxidant and hepatoprotective activities of intracellular polysaccharide from Pleurotus eryngii SI-04. Int J Biol Macromol. 2016;91:568–577.
   PubMed Web of Science ®Google Scholar
• Zhao H, Li S, Zhang J, et al. The antihyperlipidemic activities of enzymatic and acidic intracellular polysaccharides by Termitomyces albuminosus. Carbohydr Polym. 2016;151:1227–1234.
   PubMed Web of Science ®Google Scholar
• Wang KF, Yan Sui K, Guo C, et al. Improved production and antitumor activity of intracellular protein-polysaccharide from Trametes versicolor by the quorum sensing molecule-tyrosol. J Funct Foods. 2017;37:90–96.
   Web of Science ®Google Scholar
• Cao Y, Liu W, Zhao Q, et al. Comparative study on the monosaccharide compositions, antioxidant and hypoglycemic activities in vitro of intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus. Int J Biol Macromol. 2019;139:1–7.
   PubMed Web of Science ®Google Scholar
• Zhang G, Yin Q, Han T, et al. Purification and antioxidant effect of novel fungal polysaccharides from the stroma of Cordyceps kyushuensis. Ind Crops Prod. 2015;69:485–491.
   Web of Science ®Google Scholar
• Akila RM. Fermentative production of fungal chitosan, a versatile biopolymer (perspectives and its applications). Adv Appl Sci Res. 2014;5:157–170.
   Google Scholar
• Banerjee MM, Banerjee D. Fungal exopolysaccharide: production, composition and applications. Microbiol Insights. 2013;6:1–16.
   PubMedGoogle Scholar
• Lee CM, Cho EM, Yang SI, et al. Raman spectroscopy and density functional theory calculations of β-glucans and chitins in fungal cell walls. Bull Korean Chem Soc. 2013;34(3):943–945.
   Web of Science ®Google Scholar
• Ismail B, Nampoothiri KM. Exopolysaccharide production and prevention of syneresis in starch using encapsulated probiotic Lactobacillus plantarum. Food Technol Biotechnol. 2010;48:484–489.
   Web of Science ®Google Scholar
• Osińska-Jaroszuk M, Jarosz-Wilkołazka A, Jaroszuk-Ściseł J, et al. Extracellular polysaccharides from Ascomycota and Basidiomycota: production conditions, biochemical characteristics, and biological properties. World J Microbiol Biotechnol. 2015;31(12):1823–1844.
   PubMed Web of Science ®Google Scholar
• Li R, Jiang X, Guan H. Optimization of mycelium biomass and exopolysaccharides production by Hirsutella sp. in submerged fermentation and evaluation of exopolysaccharides antibacterial activity. Afr J Biotechnol. 2010;9:195–202.
   Google Scholar
• Bin Zhou L, Chen B. Bioactivities of water-soluble polysaccharides from Jisongrong mushroom: anti-breast carcinoma cell and antioxidant potential. Int J Biol Macromol. 2011;48(1):1–4.
   PubMed Web of Science ®Google Scholar
• Khan AA, Gani A, Khanday FA, et al. Biological and pharmaceutical activities of mushroom β-glucan discussed as a potential functional food ingredient. Bioact Carbohydr Diet Fibre. 2018;16:1–13.
   Google Scholar
• Ruiz-Herrera J, Ortiz-Castellanos L. Cell wall glucans of fungi. A review. Cell Surf. 2019;5:100022.
   PubMedGoogle Scholar
• Fesel PH, Zuccaro A. β-glucan: crucial component of the fungal cell wall and elusive MAMP in plants. Fungal Genet Biol. 2016;90:53–60.
   PubMed Web of Science ®Google Scholar
• Synytsya A, Novák M. Structural diversity of fungal glucans. Carbohydr Polym. 2013;92(1):792–809.
   PubMed Web of Science ®Google Scholar
• Dikit P, Methacanon P, Visessanguan W, et al. Characterization of an unexpected bioemulsifier from spent yeast obtained from Thai traditional liquor distillation. Int J Biol Macromol. 2010;47(4):465–470.
   PubMed Web of Science ®Google Scholar
• Kittisuban P, Ritthiruangdej P, Suphantharika M. Optimization of hydroxypropylmethylcellulose, yeast β-glucan, and whey protein levels based on physical properties of gluten-free rice bread using response surface methodology. LWT Food Sci Technol. 2014;57(2):738–748.
   Web of Science ®Google Scholar
• Brennan MA, Derbyshire E, Tiwari BK, et al. Integration of β-glucan fibre rich fractions from barley and mushrooms to form healthy extruded snacks. Plant Foods Hum Nutr. 2013;68(1):78–82.
   PubMed Web of Science ®Google Scholar
• Kim J, Lee M, Bae Y, et al. (1-3)(1-6)-β-glucan-enriched materials from Lentinus edodes mushroom as a high-fibre and low-calorie flour substitute for baked foods. J Sci Food Agric. 2011;91(10):1915–1919.
   PubMed Web of Science ®Google Scholar
• Viñarta SC, Molina OE, Figueroa LIC, et al. A further insight into the practical applications of exopolysaccharides from Sclerotium rolfsii. Food Hydrocoll. 2006;20(5):619–629.
   Web of Science ®Google Scholar
• Falch BH, Espevik T, Ryan L, et al. The cytokine stimulating activity of (1->3)-beta-d-glucans is dependent on the triple helix conformation. Carbohydr Res. 2000;329(3):587–596.
   PubMed Web of Science ®Google Scholar
• Wu S, Chen J. Preparation of maltotriose from fermentation broth by hydrolysis of pullulan using pullulanase. Carbohydr Polym. 2014;107:94–97.
   PubMed Web of Science ®Google Scholar
• Morris VJ. Bacterial polysaccharides. In: Stephen AM, editor. Food polysaccharides and their application. New York: Marcel Dekker; 1995. p. 341–375.
   Google Scholar
• Wolf BW, Garleb KA, Choe YS, et al. Pullulan is a slowly digested carbohydrate in humans. Human Nutr Metab. 2003;133(4):1051–1055.
   Google Scholar
• Samuelsen ABC, Schrezenmeir J, Knutsen SH. Effects of orally administered yeast-derived beta-glucans: a review. Mol Nutr Food Res. 2014;58(1):183–193.
   PubMed Web of Science ®Google Scholar
• Mirza Z, Soto ER, Dikengil F. Beta-glucan particles as vaccine adjuvant carriers. In: Kalkum M, Semis M, editors. Vaccines for invasive fungal infections: methods and protocols, methods in molecular biology. Vol. 11. New York (NY): Humana Press 2017. p. 143–157.
   Google Scholar
• Kofuji K, Huang Y, Tsubaki K, et al. Preparation and evaluation of a novel wound dressing sheet comprised of β-glucan-chitosan complex. React Funct Polym. 2010;70(10):784–789.
   Web of Science ®Google Scholar
• Giavasis I. Bioactive fungal polysaccharides as potential functional ingredients in food and nutraceuticals. Curr Opin Biotechnol. 2014;26:162–173.
   PubMed Web of Science ®Google Scholar
• Mastromarino P, Petruzziello R, Macchia S, et al. Antiviral activity of natural and semisynthetic polysaccharides on the early steps of rubella virus infection. J Antimicrob Chemother. 1997;39(3):339–345.
   PubMed Web of Science ®Google Scholar
• Li H, Bian S, Huang Y, et al. High drug loading pH-sensitive pullulan-DOX conjugate nanoparticles for hepatic targeting. J Biomed Mater Res. 2014;102(1):150–159.
   Web of Science ®Google Scholar
• Zhang HZ, Gao FP, Liu LR, et al. Pullulan acetate nanoparticles prepared by solvent diffusion method for epirubicin chemotherapy. Colloids Surf B Biointerfaces. 2009;71(1):19–26.
   PubMed Web of Science ®Google Scholar
• Du B, Bian Z, Xu B. Skin health promotion effects of natural beta-glucan derived from cereals and microorganisms: a review. Phytother Res. 2014;28(2):159–166.
   PubMed Web of Science ®Google Scholar
• Kagimura FY, da Cunha MAA, Barbosa AM, et al. Biological activities of derivatized d-glucans: a review. Int J Biol Macromol. 2015;72:588–598.
   PubMed Web of Science ®Google Scholar
• Leathers TD. Biotechnological production and applications of pullulan. Appl Microbiol Biotechnol. 2003;62(5–6):468–473.
   PubMed Web of Science ®Google Scholar
• Rekha MR, Sharma CP. Pullulan as a promising biomaterial for biomedical applications: a perspective. Trends Biomater Artif Organs. 2007;20:111–116.
   Google Scholar
• Singh RS, Saini GK, Kennedy JF. Pullulan: microbial sources, production and applications. Carbohydr Polym. 2008;73(4):515–531.
   PubMed Web of Science ®Google Scholar
• Mohan K, Ravichandran S, Muralisankar T, et al. Potential uses of fungal polysaccharides as immunostimulants in fish and shrimp aquaculture: a review. Aquaculture. 2019;500(2019):250–263.
   Google Scholar
• Sveistrup M, van Mastrigt F, Norrman J, et al. Viability of biopolymers for enhanced oil recovery. J Dispers Sci Technol. 2016;37(8):1160–1169.
   Web of Science ®Google Scholar
• Zhu F, Du B, Xu B. A critical review on production and industrial applications of beta-glucans. Food Hydrocoll. 2016;52:275–288.
   Web of Science ®Google Scholar
• Bai J, Ren Y, Li Y, et al. Physiological functionalities and mechanisms of β-glucans. Trends Food Sci Technol. 2019;88:57–66.
   Web of Science ®Google Scholar
• Schmid J, Meyer V, Sieber V. Scleroglucan: biosynthesis, production and application of a versatile hydrocolloid. Appl Microbiol Biotechnol. 2011;91(4):937–947.
   PubMed Web of Science ®Google Scholar
• Gientka L, Bzducha-Wróbel A, Stasiak-Różańska L, et al. The exopolysaccharides biosynthesis by Candida yeast depends on carbon sources. Electron J Biotechnol. 2016;22:31–37.
   Web of Science ®Google Scholar
• Rodrigues ML, Franzen AJ, Nimrichter L, et al. Vesicular mechanisms of traffic of fungal molecules to the extracellular space. Curr Opin Microbiol. 2013;16(4):414–420.
   PubMed Web of Science ®Google Scholar
• Rodrigues ML, Nosanchuk JD, Schrank A, et al. Vesicular transport systems in fungi. Future Microbiol. 2011;6(11):1371–1381.
   PubMed Web of Science ®Google Scholar
• Hu X, Pang X, Wang PG, et al. Isolation and characterization of an antioxidant exopolysaccharide produced by Bacillus sp. S-1 from Sichuan pickles. Carbohydr Polym. 2019;204:9–16.
   PubMed Web of Science ®Google Scholar
• Kheni K, Vyas TK. Characterization of exopolysaccharide produced by Ganoderma sp TV1 and its potential as antioxidant and anticancer agent. J Biol Act Prod Nat. 2017;7(2):72–80.
   Google Scholar
• Paynich ML, Jones-Burrage SE, Knight KL. Exopolysaccharide from Bacillus subtilis induces anti-inflammatory M2 macrophages that prevent T cell-mediated disease. J Immunol. 2017;198:2689–2698.
   PubMed Web of Science ®Google Scholar
• Liu C, Lu J, Lu L, et al. Isolation, structural characterization and immunological activity of an exopolysaccharide produced by Bacillus licheniformis 8-37-0-1. Bioresour Technol. 2010;101(14):5528–5533.
   PubMed Web of Science ®Google Scholar
• Ates O. Systems biology of microbial exopolysaccharides production. Front Bioeng Biotechnol. 2015;3:200–216.
   PubMed Web of Science ®Google Scholar
• Chang I, Kharis A, Im J, et al. Geoderma soil treatment using microbial biopolymers for anti-desertification purposes. Geoderma. 2015;253–254:39–47.
   Web of Science ®Google Scholar
• Todero I, Confortin TC, Luft L, et al. Concentration of exopolysaccharides produced by Fusarium fujikuroi and application of bioproduct as an effective bioherbicide. Environ Technol. 2019;1–8. DOI:https://doi.org/10.1080/09593330.2019.1580775
   Google Scholar
• Luft L, Confortin TC, Todero I, et al. Different techniques for concentration of extracellular biopolymers with herbicidal activity produced by Phoma sp different techniques for concentration of extracellular biopolymers with herbicidal activity produced by Phoma sp. Environ Technol. 2019;1–10. DOI:https://doi.org/10.1080/09593330.2019.1669720
   Google Scholar
• Lei S, Feng Edmund T. Polysaccharides, microbial. In: Roitberg BD, editor. Reference module in life sciences; Elsevier Inc. 2017. p. 482–494.
   Google Scholar
• Zheng H, Hao M, Liu W, et al. Foam fractionation for the concentration of exopolysaccharides produced by repeated batch fermentation of Cordyceps militaris. Sep Purif Technol. 2019;210:682–689.
   Web of Science ®Google Scholar
• Roca C, Chagas B, Farinha I, et al. Production of yeast chitin-glucan complex from biodiesel industry byproduct. Process Biochem. 2012;47(11):1670–1675.
   Web of Science ®Google Scholar
• New N, Furuike T, Tamura H. Production, properties and applications of fungal cell wall polysaccharides: chitosan and glucan. Adv Polym Sci. 2011;244:187–207.
   Web of Science ®Google Scholar
• Cota-Arriola O, Onofre Cortez-Rocha M, Burgos-Hernández A, et al. Controlled release matrices and micro/nanoparticles of chitosan with antimicrobial potential: development of new strategies for microbial control in agriculture. J Sci Food Agric. 2013;93(7):1525–1536.
   PubMed Web of Science ®Google Scholar
• Abdel-Gawad KM, Hifney AF, Fawzy MA, et al. Technology optimization of chitosan production from Aspergillus niger biomass and its functional activities. Food Hydrocoll. 2017;63:593–601.
   Web of Science ®Google Scholar
• Wu J, Niu Y, Jiao Y, et al. Fungal chitosan from Agaricus bisporus (Lange) Sing. Chaidam increased the stability and antioxidant activity of liposomes modified with biosurfactants and loading betulinic acid. Int J Biol Macromol. 2019;123:291–299.
   PubMed Web of Science ®Google Scholar
• Rungsardthong V, Wongvuttanakul N, Kongpien N, et al. Application of fungal chitosan for clarification of apple juice. Process Biochem. 2006;41(3):589–593.
   Web of Science ®Google Scholar
• Tayel AA, Ibrahim SIA, Al-Saman MA, et al. Production of fungal chitosan from date wastes and its application as a biopreservative for minced meat. Int J Biol Macromol. 2014;69:471–475.
   PubMed Web of Science ®Google Scholar
• Alsaggaf MS, Moussa SH, Tayel AA. Application of fungal chitosan incorporated with pomegranate peel extract as edible coating for microbiological, chemical and sensorial quality enhancement of Nile tilapia fillets. Int J Biol Macromol. 2017;99:499–505.
   PubMed Web of Science ®Google Scholar
• Pereira RB, Lúcio M, De Resende V, et al. Ativação de defesa em cacaueiro contra a murcha-de-verticílio por extratos naturais e acibenzolar-S-metil. Pesq Agropec Bras. 2008;43(2):171–178.
   Google Scholar
• Alsharari SF, Tayel AA, Moussa SH. Soil emendation with nano-fungal chitosan for heavy metals biosorption. Int J Biol Macromol. 2018;118(Pt B):2265–2268.
   PubMed Web of Science ®Google Scholar
• Alshubaily FA. Enhanced antimycotic activity of nanoconjugates from fungal chitosan and Saussurea costus extract against resistant pathogenic Candida strains. Int J Biol Macromol. 2019;141:499–503.
   PubMed Web of Science ®Google Scholar
• Nge KL, Nwe N, Chandrkrachang S, et al. Chitosan as a growth stimulator in orchid tissue culture. Plant Sci. 2006;170(6):1185–1190.
   Web of Science ®Google Scholar
• Mohd Nasir N, Mohd Yunos FH, Wan Jusoh HH, et al. Subtopic: advances in water and wastewater treatment harvesting of Chlorella sp. microalgae using Aspergillus niger as bio-flocculant for aquaculture wastewater treatment. J Environ Manage. 2019;249:109373.
   PubMed Web of Science ®Google Scholar
• Almutairi FM, El Rabey HA, Tayel AA, et al. Augmented anticancer activity of curcumin loaded fungal chitosan nanoparticles. Int J Biol Macromol. 2019;155:861–867.
   Web of Science ®Google Scholar
• Zhu L, Li J, Mai J, et al. Ultrasound-assisted synthesis of chitosan from fungal precursors for biomedical applications. Chem Eng J. 2019;357:498–507.
   Web of Science ®Google Scholar
• Anbazhagan S, Thangavelu KP. Application of tetracycline hydrochloride loaded-fungal chitosan and Aloe vera extract based composite sponges for wound dressing. J Adv Res. 2018;14:63–71.
   PubMed Web of Science ®Google Scholar
• Tianwei T, Binwu W, Xinyuan S. Separation of chitosan from Penicillium chrysogenum mycelium and its applications. J Bioact Compat Polym. 2002;17(3):173–182.
   Web of Science ®Google Scholar
• Tajdini F, Ali M, Nafissi-Varcheh N, et al. Production, physiochemical and antimicrobial properties of fungal chitosan from Rhizomucor miehei and Mucor racemosus. Int J Biol Macromol. 2010;47(2):180–183.
   PubMed Web of Science ®Google Scholar
• Nwe N, Furuike T, Tamura H. The mechanical and biological properties of chitosan scaffolds for tissue regeneration templates are significantly enhanced by chitosan from Gongronella butleri. Materials (Basel). 2009;2(2):374–398.
   Web of Science ®Google Scholar
• Farias JM, Stamford TCM, Resende AHM, et al. Mouthwash containing a biosurfactant and chitosan: an eco-sustainable option for the control of cariogenic microorganisms. Int J Biol Macromol. 2019;129:853–860.
   PubMed Web of Science ®Google Scholar
• Kučera J. Fungal mycelium – the source of chitosan for chromatography. J Chromatogr B Anal Technol Biomed Life Sci. 2004;808(1):69–73.
   PubMed Web of Science ®Google Scholar
• Biniarz P, Łukaszewicz M, Janek T. Screening concepts, characterization and structural analysis of microbial-derived bioactive lipopeptides: a review. Crit Rev Biotechnol. 2017;37(3):393–410.
   PubMed Web of Science ®Google Scholar
• Gudiña EJ, Rangarajan V, Sen R, et al. Potential therapeutic applications of biosurfactants. Trends Pharmacol Sci. 2013;34(12):667–675.
   PubMed Web of Science ®Google Scholar
• Ishaq U, Akram MS, Iqbal Z, et al. Production and characterization of novel self-assembling biosurfactants from Aspergillus flavus. J Appl Microbiol. 2015;119(4):1035–1045.
   PubMed Web of Science ®Google Scholar
• Kiran GS, Hema TA, Gandhimathi R, et al. Optimization and production of a biosurfactant from the sponge-associated marine fungus Aspergillus ustus MSF3. Colloids Surf B Biointerfaces. 2009;73(2):250–256.
   PubMed Web of Science ®Google Scholar
• dos Reis CBL, Morandini LMB, Bevilacqua CB, et al. First report of the production of a potent biosurfactant with α,β-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation. Brazilian J Microbiol. 2018;49:1–8.
   Web of Science ®Google Scholar
• Qazi MA, Subhan M, Fatima N, et al. Role of biosurfactant produced by Fusarium sp. BS-8 in enhanced oil recovery (EOR) through sand pack column. Int J Biosci Biochem Bioinform. 2013;30:1065–1075.
   Google Scholar
• Qazi MA, Kanwal T, Jadoon M, et al. Isolation and characterization of a biosurfactant-producing Fusarium sp. BS-8 from oil contaminated soil. Biotechnol Prog. 2014;30(5):1065–1075.
   PubMed Web of Science ®Google Scholar
• Morita T, Ishibashi Y, Hirose N, et al. Production and characterization of a glycolipid biosurfactant, mannosylerythritol lipid B, from sugarcane juice by Ustilago scitaminea NBRC 32730. Biosci Biotechnol Biochem. 2011;75(7):1371–1376.
   PubMed Web of Science ®Google Scholar
• Spoeckner S, Wray V, Nimtz M, et al. Glycolipids of the smut fungus Ustilago maydis from cultivation on renewable resources. Appl Microbiol Biotechnol. 1999;51(1):33–39.
   Web of Science ®Google Scholar
• Gautam G, Mishra V, Verma P, et al. A cost effective strategy for production of bio-surfactant from locally isolated Penicillium chrysogenum SNP5 and its applications. J Bioprocess Biotechn. 2014;4(6):1.
   Google Scholar
• Lourenço LA, Alberton Magina MD, Tavares LBB, et al. Biosurfactant production by Trametes versicolor grown on two-phase olive mill waste in solid-state fermentation. Environ Technol (UK). 2017;3330:1–11.
   Google Scholar
• Velioglu Z, Ozturk Urek R. Optimization of cultural conditions for biosurfactant production by Pleurotus djamor in solid state fermentation. J Biosci Bioeng. 2015;120(5):526–531.
   PubMed Web of Science ®Google Scholar
• Konishi M, Fukuoka T, Morita T, et al. Production of new types of sophorolipids by Candida batistae. J Oleo Sci. 2008;57(6):359–369.
   PubMed Web of Science ®Google Scholar
• Santos DKF, Rufino RD, Luna JM, et al. Synthesis and evaluation of biosurfactant produced by Candida lipolytica using animal fat and corn steep liquor. J Pet Sci Eng. 2013;105:43–50.
   Web of Science ®Google Scholar
• Cavalero DA, Cooper DG. The effect of medium composition on the structure and physical state of sophorolipids produced by Candida bombicola ATCC 22214. J Biotechnol. 2003;103(1):31–41.
   PubMed Web of Science ®Google Scholar
• Thanomsub B, Watcharachaipong T, Chotelersak K, et al. Monoacylglycerols: glycolipid biosurfactants produced by a thermotolerant yeast, Candida ishiwadae. J Appl Microbiol. 2004;96(3):588–592.
   PubMed Web of Science ®Google Scholar
• Garg M, Priyanka Chatterjee, M. Isolation, characterization and antibacterial effect of biosurfactant from Candida parapsilosis. Biotechnol Rep. 2018;18:e00251.
   Google Scholar
• Senthil Balan S, Ganesh Kumar C, Jayalakshmi S. Physicochemical, structural and biological evaluation of Cybersan (trigalactomargarate), a new glycolipid biosurfactant produced by a marine yeast, Cyberlindnera saturnus strain SBPN-27. Process Biochem. 2019;80:171–180.
   Web of Science ®Google Scholar
• Alt K, Sireesha NG, Akg A, et al. Production optimization of rhamnolipid biosurfactant by Streptomyces coelicoflavus (NBRC 15399 T) using Plackett–Burman design. Eur J Biotechnol Biosci. 2014;1:7–13.
   Google Scholar
• Coronel-León J, de Grau G, Grau-Campistany A, et al. Biosurfactant production by AL 1.1, a Bacillus licheniformis strain isolated from Antarctica: production, chemical characterization and properties. Ann Microbiol. 2015;65(4):2065–2078.
   Web of Science ®Google Scholar
• Ma Z, Hu J. Production and characterization of iturinic lipopeptides as antifungal agents and biosurfactants produced by a marine Pinctada martensii-derived Bacillus mojavensis B0621A. Appl Biochem Biotechnol. 2014;173(3):705–715.
   PubMed Web of Science ®Google Scholar
• Varadavenkatesan T, Murty VR. Production of a lipopeptide biosurfactant by a novel Bacillus sp. and its applicability to enhanced oil recovery. ISRN Microbiol. 2013;2013:621519.
   PubMedGoogle Scholar
• Gogoi D, Bhagowati P, Gogoi P, et al. Structural and physico-chemical characterization of a dirhamnolipid biosurfactant purified from: Pseudomonas aeruginosa: application of crude biosurfactant in enhanced oil recovery. RSC Adv. 2016;6(74):70669–70681.
   Web of Science ®Google Scholar
• Jadhav M, Kagalkar A, Jadhav S, et al. Isolation, characterization, and antifungal application of a biosurfactant produced by Enterobacter sp. MS16. Eur J Lipid Sci Technol. 2011;113(11):1347–1356.
   Web of Science ®Google Scholar
• Bages-Estopa S, White DA, Winterburn JB, et al. Production and separation of a trehalolipid biosurfactant. Biochem Eng J. 2018;139:85–94.
   Web of Science ®Google Scholar
• Bhardwaj G. Biosurfactants from fungi: a review. J Pet Environ Biotechnol. 2013;4:1–6.
   Google Scholar
• Hazra C, Kundu D, Chaudhari A. Lipopeptide biosurfactant from Bacillus clausii BS02 using sunflower oil soapstock: evaluation of high throughput screening methods, production, purification, characterization and its insecticidal activity. RSC Adv. 2015;5(4):2974–2982.
   Web of Science ®Google Scholar
• Geys R, Soetaert W, Van Bogaert I. Biotechnological opportunities in biosurfactant production. Curr Opin Biotechnol. 2014;30:66–72.
   PubMed Web of Science ®Google Scholar
• Mnif I, Ghribi D. Glycolipid biosurfactants: main properties and potential applications in agriculture and food industry. J Sci Food Agric. 2016;96(13):4310–4320.
   PubMed Web of Science ®Google Scholar
• Desai JD, Banat IM. Microbial production of surfactants and their commercial potential. Microbiol Mol Biol Rev. 1997;61(1):47–64.
   PubMed Web of Science ®Google Scholar
• Abdel-Mawgoud AM, Stephanopoulos G. Simple glycolipids of microbes: chemistry, biological activity and metabolic engineering. Synth Syst Biotechnol. 2018;3(1):3–19.
   PubMed Web of Science ®Google Scholar
• Batrakov SG, Konova IV, Sheichenko VI, et al. Glycolipids of the filamentous fungus Absidia corymbifera. Chem Phys Lipids. 2003;123(2):157–164.
   PubMed Web of Science ®Google Scholar
• Liu Y, Koh CMJ, Ji L. Bioconversion of crude glycerol to glycolipids in Ustilago maydis. Bioresour Technol. 2011;102(4):3927–3933.
   PubMed Web of Science ®Google Scholar
• Teichmann B, Linne U, Hewald S, et al. A biosynthetic gene cluster for a secreted cellobiose lipid with antifungal activity from Ustilago maydis. Mol Microbiol. 2007;66(2):525–533.
   PubMed Web of Science ®Google Scholar
• Mnif I, Ghribi D. Lipopeptide surfactants: production, recovery and pore forming capacity. Peptides. 2015;71:100–112.
   PubMed Web of Science ®Google Scholar
• Jain RM, Mody K, Joshi N, et al. Production and structural characterization of biosurfactant produced by an alkaliphilic bacterium, Klebsiella sp.: evaluation of different carbon sources. Colloids Surf B Biointerfaces. 2013;108:199–204.
   PubMed Web of Science ®Google Scholar
• Singh SP, Bharali P, Konwar BK. Optimization of nutrient requirements and culture conditions for the production of rhamnolipid from Pseudomonas aeruginosa (MTCC 7815) using Mesua ferrea seed oil. Indian J Microbiol. 2013;53(4):467–476.
   PubMed Web of Science ®Google Scholar
• Luna-Velasco MA, Esparza-García F, Cañízares-Villanueva RO, et al. Production and properties of a bioemulsifier synthesized by phenanthrene-degrading Penicillium sp. Process Biochem. 2007;42(3):310–314.
   Web of Science ®Google Scholar
• Rebello S, Asok AK, Mundayoor S, et al. Surfactants: toxicity, remediation and green surfactants. Environ Chem Lett. 2014;12(2):275–287.
   Web of Science ®Google Scholar
• Araújo TR, Lúcia C, Petkowicz DO, et al. Biopolymer production using fungus Mucor racemosus Fresenius and glycerol as substrate. Polímeros. 2016;26(2):144–151.
   Google Scholar
• Vecino X, Cruz JM, Moldes AB, et al. Critical reviews in biotechnology biosurfactants in cosmetic formulations: trends and challenges. Crit Rev Biotechnol. 2017;37(7):911–913.
   PubMed Web of Science ®Google Scholar
• Research and Markets. Biosurfactants – global market outlook (2017–2026); 2018; [cited 2020 Apr 28]. Available from: https://www.researchandmarkets.com/reports/4541406/biosurfactants-global-market-outlook-2017-2026#pos-0
   Google Scholar
• Grand View Research Inc. Biosurfactants market by product (market research report code: GVR20); 2014; [cited 2020 Apr 28]. Available from: http://www.grandviewresearch.com/industry-analysis/biosurfactants-industry
   Google Scholar
• Dhanarajan G, Sen R. Cost analysis of biosurfactant production from a scientist’s perspective. In: Naim Kosaric, Fazilet Vardar Sukan, editors. Biosurfactants. Production and utilization-processes, technologies, and economics. Vol. 10. Boca Raton (FL): CRC Press; 2014. p. 153–161.
   Google Scholar
• Makkar RS, Cameotra SS, Banat IM. Advances in utilization of renewable substrates for biosurfactant production. AMB Express. 2011;1(1):5.
   PubMedGoogle Scholar
• Soares SRCF, de Almeida DG, Brasileiro PPF, et al. Production, formulation and cost estimation of a commercial biosurfactant. Biodegradation. 2019;30(4):191–201.
   PubMed Web of Science ®Google Scholar
• Singh P, Patil Y, Rale V. Biosurfactant production: emerging trends and promising strategies. J Appl Microbiol. 2019;126(1):2–13.
   PubMed Web of Science ®Google Scholar
• Daverey A, Pakshirajan K. Production, characterization, and properties of sophorolipids from the yeast Candida bombicola using a low-cost fermentative medium. Appl Biochem Biotechnol. 2009;158(3):663–674.
   PubMed Web of Science ®Google Scholar
• Haddad NIA, Liu X, Yang S, et al. Surfactin isoforms from Bacillus subtilis HSO121: separation and characterization. Protein Pept Lett. 2008;15(3):265–269.
   PubMed Web of Science ®Google Scholar
• Chen HL, Chen YS, Juang RS. Separation of surfactin from fermentation broths by acid precipitation and two-stage dead-end ultrafiltration processes. J Membr Sci. 2007;299(1–2):114–121.
   Web of Science ®Google Scholar
• Freitas F, Alves VD, Reis MAM. Advances in bacterial exopolysaccharides: from production to biotechnological applications. Trends Biotechnol. 2011;29(8):388–398.
   PubMed Web of Science ®Google Scholar
• Saharan BS, Sahu RK, Sharma D. A review on biosurfactants: fermentation, current developments and perspectives. Genet Eng Biotechnol J. 2012;2011:1–14.
   Google Scholar
• Park JP, Kim SW, Hwang HJ, et al. Stimulatory effect of plant oils and fatty acids on the exo-biopolymer production in Cordyceps militaris. Enzyme Microb Technol. 2002;31(3):250–255.
   Web of Science ®Google Scholar
• Fontes GC, Amaral PFFE, Coelho MAZ. Biosurfactants production by yeasts. Quím Nova. 2008;31(8):2091–2323.
   Web of Science ®Google Scholar
• Santos DKF, Rufino RD, Luna JM, et al. Biosurfactants: multifunctional biomolecules of the 21st century. Int J Mol Sci. 2016;17(3):401–431.
   PubMed Web of Science ®Google Scholar
• Nurfarahin AH, Mohamed MS, Phang LY. Culture medium development for microbial-derived surfactants production—an overview. Molecules. 2018;23(5):1049.
   Web of Science ®Google Scholar
• Fang QH, Zhong JJ. Effect of initial pH on production of ganoderic acid and polysaccharide by submerged fermentation of Ganoderma lucidum. Process Biochem. 2002;37(7):769–774.
   Web of Science ®Google Scholar
• Van Bogaert INA, Zhang J, Soetaert W. Microbial synthesis of sophorolipids. Process Biochem. 2011;46(4):821–833.
   Web of Science ®Google Scholar
• Coutte F, Lecouturier D, Dimitrov K, et al. Microbial lipopeptide production and purification bioprocesses, current progress and future challenges. Biotechnol J. 2017;12(7):1600566.
   Web of Science ®Google Scholar
• Satpute SK, Banpurka AG, Dhakephalkar PK, et al. Methods for investigating biosurfactants and bioemulsifiers: a review. Crit Rev Biotechnol. 2010;30(2):127–144.
   PubMed Web of Science ®Google Scholar
• Li SC, Yang XM, Le Ma H, et al. Purification, characterization and antitumor activity of polysaccharides extracted from Phellinus igniarius mycelia. Carbohydr Polym. 2015;133:24–30.
   PubMed Web of Science ®Google Scholar
• Tang YJ, Zhong JJ. Fed-batch fermentation of Ganoderma lucidum for hyperproduction of polysaccharide and ganoderic acid. Enzyme Microb Technol. 2002;31(1–2):20–28.
   Web of Science ®Google Scholar
• Zhang H, Ma H, Liu W, et al. Ultrasound enhanced production and antioxidant activity of polysaccharides from mycelial fermentation of Phellinus igniarius. Carbohydr Polym. 2014;113:380–387.
   PubMed Web of Science ®Google Scholar
• Sourki AH, Koocheki A, Elahi M. Ultrasound-assisted extraction of β-d-glucan from hull-less barley: assessment of physicochemical and functional properties. Int J Biol Macromol. 2017;95:462–475.
   PubMed Web of Science ®Google Scholar
• Formenti LR, Nørregaard A, Bolic A, et al. Challenges in industrial fermentation technology research. Biotechnol J. 2014;9(6):727–738.
   PubMed Web of Science ®Google Scholar
• Posch AE, Herwig C, Spadiut O. Science-based bioprocess design for filamentous fungi. Trends Biotechnol. 2013;31(1):37–44.
   PubMed Web of Science ®Google Scholar
• Ezeji T, Qureshi N, Blaschek HP. Butanol production from agricultural residues: impact of degradation products on Clostridium beijerinckii growth and butanol fermentation. Biotechnol Bioeng. 2007;97(6):1460–1469.
   PubMed Web of Science ®Google Scholar
• Tajsoleiman T, Mears L, Krühne U, et al. An industrial perspective on scale-down challenges using miniaturized bioreactors. Trends Biotechnol. 2019;37(7):697–706.
   PubMed Web of Science ®Google Scholar
• Seviour RJ, McNeil B, Fazenda ML, et al. Operating bioreactors for microbial exopolysaccharide production. Crit Rev Biotechnol. 2011;31(2):170–185.
   PubMed Web of Science ®Google Scholar
• Ray SG, Ghangrekar MM. Process for treatment of environmentally hazardous grain based distillery waste water, involves using Aspergillus awamori fungus to convert waste water into fungal biomass and fungal pre-treated effluent. Patent IN201400822-I2. 2014.
   Google Scholar
• Lou W, Zeng Y, Zong M, et al. Preparing highly immunologically active endophytic fungus polysaccharide of Dendrobium candidum involves inoculating the activated Dendrobium candidum endophytic fungal seed solution in a sterilized solid medium. Patent CN109234332-A. 2019.
   Google Scholar
• Mukhopadhyay SK, Chatterjee S, Mishra A, et al. Producing bioactive exopolysaccharides (EPS) used to obtain oligosaccharide used e.g. as anti-cancer agent, comprises e.g. inoculating basal salt medium with Thelebolus species and incubating to get EPS, filtering medium and adding alcohol. Patent IN201300999-I2. 2013.
   Google Scholar
• Kaplan-Bie JH, Bonesteel IT, Greetham L, et al. Growing biopolymer material involves providing multiple of containers, each container defining cavity containing growth media includes nutritive substrate and fungus, placing multiple of containers in closed incubation chamber. Patent WO2019099474-A1. 2019.
   Google Scholar
• Dai Z, Ai X, Yang Z. Biopolymer fertilizer used as cotton special fertilizer, comprises e.g. urea, calcium-magnesium-phosphate fertilizer, nitrophosphate, ammonium chloride, ammonium sulfate, monoammonium phosphate, potassium sulfate and potassium chloride. Patent CN109293452-A. 2019.
   Google Scholar
• Rollie S, Kaeppler T, Schalb C, et al. Preparing aqueous solution comprising beta-glucan used e.g. in polymer flooding, comprises fermenting fungal strain secreting beta-glucan into aqueous culture medium to form fermentation broth, adding acid to broth, and filtering the broth. Patent WO2016087521-A1. 2016.
   Google Scholar
• Kaplan-Bie JH, Kaplan-Bei JH. Processed mycological biopolymer material comprises fungal mycelium free of any stipe, cap or spores and in having elasticity, which is prepared by treating tissue with organic solvent solution for time sufficient to permit permeability. Patent US2018282529-A1. 2018.
   Google Scholar
• Briechle S, Hollmann R, Kaeppler T, et al. Fermenting fungal strains secreting glucan with beta-1,3-glycosidically linked main chain and side groups bonded in cascade of tank, involves fermenting strains in first aqueous medium and transferring first mixture to second tank. Patent WO2016091892-A1. 2016.
   Google Scholar
• Pontarolo R, Picheth GF, Camarozano AC, et al. Micro-vesicle used in biomedical imaging, comprises gaseous core and composite layer of phospholipids 1,2-distearoyl-sn-glycero-3-phosphocholine and bio-polymers comprising polysaccharides, hyaluronic acid, heparin and alginate. Patent BR102014028264-A2. 2014.
   Google Scholar
• Assis LTMA, Gonçalves O. Producing proteinaceous biopolymer for use in biomedical field, preferably advanced medical biotechnology bone transplant, involves preparing microbial mixture containing mangrove contaminated with oil, crude glycerin, and castor cake. Patent BR201105932-A2. 2011.
   Google Scholar
• Dierickx W, De Cuyper D, Gunde-Cimerman N, et al. Polymer product, useful as polymer film and for the production of plastic and textile materials, comprises polymer in form of granules, multidimensional structure containing amphiphilic molecules e.g. lipids, surfactants or polymers. Patent WO2012145803-A2. 2012.
   Google Scholar
• Vanangamudi SS, Srinivasan M, Chulliel NN, et al. Cream, useful to treat inflammation, comprises fusidic acid, mometasone furoate, clotrimazole, and a biopolymer, preferably chitosan, in a cream base containing an emulsifier, a preservative, a waxy material, a cosolvent, an acid and water. Patent WO2012049542-A1. 2012.
   Google Scholar
• Peij VNNM, Hans M, Beishuizen M, et al. Producing a compound of interest by microbial fermentation, comprises providing microbial host cell, culturing cell under conditions conducive to expression, and isolating compound of interest from culture medium. Patent WO2012001169-A1. 2012.
   Google Scholar
• Paik J, Haley H. Microbial stable emulsion comprises dispersion media having lower water activity, at least one dispersant having higher water binding capacity, and a lipophilic dispersed phase. Patent WO2011011077-A2. 2011.
   Google Scholar
• Yuan T. Complex useful for medical devices, biomedicine, beauty make-up and food care comprises beta-glucan and glucomannan. Patent CN108938455-A. 2019.
   Google Scholar
• Thevenieau F, Bourdillon A, Durand A, et al. Use of filamentous fungal strain for fermentative preparation of fermented solid edible food product for animal or human consumption for prevention or treatment of e.g. allergy from by-product of plant. Patent WO2015101650-A2. 2015.
   Google Scholar
• Yin S, Xie P, Qu H. Compound bioemulsifier used in oil sludge treatment, comprises concentrated mixed fermentation waste liquid and sophorolipid. Patent CN109876731-A. 2019.
   Google Scholar
• Sarubbo LA, Diniz Rufino R, Moura J, et al. Producing biosurfactant used as bioemulsifier for vegetable oils and petrochemicals industry, involves culturing Candida guilliermondii in culture medium, which comprises waste vegetable oil, sugar and corn refining residues. Patent BR102012023115-A2. 2012.
   Google Scholar
• Farmer S, Alibek K, Mazumder S, et al. Mass-producing fungal strain and/or fungal growth by-product, comprising e.g. inoculating liquid growth medium with fungal strain, and suspending particulate anchoring carrier in liquid growth medium as site for nucleating fungal growth. Patent WO2019140093-A1. 2019.
   Google Scholar