Immobilized enzyme for screening and identification of anti-diabetic components from natural products by ligand fishing

References

• Ellahham S. Artificial intelligence: the future for diabetes care. Am J Med. 2020;133(8):895–900.
   PubMed Web of Science ®Google Scholar
• Magkos F, Hjorth MF, Astrup A. Diet and exercise in the prevention and treatment of type 2 diabetes mellitus. Nat Rev Endocrinol. 2020;16(10):545–555.
   PubMed Web of Science ®Google Scholar
• Halimi S. Is a new therapeutic class justified in the treatment of type 2 diabetes? Ann Endocrinol (Paris). 2002;63(2 Pt 2):1S7–111.
   PubMed Web of Science ®Google Scholar
• Kerru N, Singh-Pillay A, Awolade P, et al. Current anti-diabetic agents and their molecular targets: a review. Eur J Med Chem. 2018;152:436–488.
   PubMed Web of Science ®Google Scholar
• Heacock PM, Hertzler SR, Williams JA, et al. Effects of a medical food containing an herbal alpha-glucosidase inhibitor on postprandial glycemia and insulinemia in healthy adults. J Am Diet Assoc. 2005;105(1):65–71.
   PubMedGoogle Scholar
• Alagesan K, Thennarasu P, Kumar V, et al. Identification of α-glucosidase inhibitors from Psidium guajava leaves and Syzygium cumini Linn. Seeds. Int J Pharm Sci Res. 2012;3:316–322.
   Google Scholar
• de la Garza AL, Milagro FI, Boque N, et al. Natural inhibitors of pancreatic lipase as new players in obesity treatment. Planta Med. 2011;77(8):773–785.
   PubMed Web of Science ®Google Scholar
• Li Y, Xu J, Chen Y, et al. Screening of inhibitors of glycogen synthase kinase-3β from traditional Chinese medicines using enzyme-immobilized magnetic beads combined with high-performance liquid chromatography. J Chromatogr A. 2015;1425:8–16.
   PubMed Web of Science ®Google Scholar
• Combs AP. Recent advances in the discovery of competitive protein tyrosine phosphatase 1B inhibitors for the treatment of diabetes, obesity, and cancer. J Med Chem. 2010;53(6):2333–2344.
   PubMed Web of Science ®Google Scholar
• Gyémánt G, Kandra L, Nagy V, et al. Inhibition of human salivary alpha-amylase by glucopyranosylidene-spiro-thiohydantoin. Biochem Biophys Res Commun. 2003;312(2):334–339.
   PubMed Web of Science ®Google Scholar
• Liu DM, Chen J, Shi YP. α-Glucosidase immobilization on chitosan-enriched magnetic composites for enzyme inhibitors screening. Int J Biol Macromol. 2017;105 (Pt 1):308–316.
   PubMedGoogle Scholar
• Cen Y, Xiao A, Chen X, et al. Screening and separation of α-amylase inhibitors from Solanum nigrum with amylase-functionalized magnetic graphene oxide combined with high-speed counter-current chromatography. J Sep Sci. 2017;40(24):4780–4787.
   PubMed Web of Science ®Google Scholar
• Singh NS, Ravichandran S, Spelman K, et al. The identification of a novel SIRT6 modulator from Trigonella foenum-graecum using ligand fishing with protein coated magnetic beads. J Chromatogr B Analyt Technol Biomed Life Sci. 2014;968:105–111.
   PubMed Web of Science ®Google Scholar
• Benalla W, Bellahcen S, Bnouham M. Antidiabetic medicinal plants as a source of alpha glucosidase inhibitors. Curr Diabetes Rev. 2010;6(4):247–254.
   PubMedGoogle Scholar
• Soong JH, Yun L, Kim EK. Therapeutic phytogenic compounds for obesity and diabetes. Int J Mol Sci. 2014;15(11):21505–21537.
   PubMed Web of Science ®Google Scholar
• Xie W, Du L. Diabetes is an inflammatory disease: evidence from traditional Chinese medicines. Diabetes Obes Metab. 2011;13(4):289–301.
   PubMed Web of Science ®Google Scholar
• Kalita D, Holm DG, LaBarbera DV, et al. Inhibition of α-glucosidase, α-amylase, and aldose reductase by potato polyphenolic compounds. PLoS One. 2018;13(1):e0191025.
   PubMed Web of Science ®Google Scholar
• Qing LS, Shan XQ, Xu XM, et al. Rapid probe and isolation of bioactive compounds from Dioscorea panthaica using human serum albumin functionalized magnetic nano-particles (HSA-MNPs)-based ligand fishing coupled with electrospray ionization mass spectrometry. Rapid Commun Mass Spectrom. 2010;24(22):3335–3339.
   PubMed Web of Science ®Google Scholar
• Ye N, Kou X, Shen J, et al. Metal-organic frameworks: a new platform for enzyme immobilization. Chembiochem. 2020;21(18):2585–2590.
   PubMed Web of Science ®Google Scholar
• Mateo C, Palomo JM, Fernandez-Lorente G, et al. Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme Microb Technol. 2007;40(6):1451–1463.
   Web of Science ®Google Scholar
• Hu X, Zhao X, Hwang H. Comparative study of immobilized Trametes versicolor laccase on nanoparticles and kaolinite. Chemosphere. 2007;66(9):1618–1626.
   PubMed Web of Science ®Google Scholar
• Fernández-Fernández M, Sanromá MA, Moldes D. Recent developments and applications of immobilized laccase. Biotechnol Adv. 2013;31(8):1808–1825.
   PubMed Web of Science ®Google Scholar
• Antony N, Balachandran S, Mohanan PV. Immobilization of diastase α-amylase on nano zinc oxide. Food Chem. 2016;211:624–630.
   PubMed Web of Science ®Google Scholar
• Mohamad NR, Marzuki NHC, Buang NA, et al. An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes. Biotechnol Biotechnol Equip. 2015;29(2):205–220.
   PubMed Web of Science ®Google Scholar
• Pavel IA, Prazeres SF, Montalvo G, et al. Effect of meso vs macro size of hierarchical porous silica on the adsorption and activity of immobilized β-galactosidase. Langmuir. 2017;33(13):3333–3340.
   PubMed Web of Science ®Google Scholar
• Manoel EA, dos Santos JCS, Freire DMG, et al. Immobilization of lipases on hydrophobic supports involves the open form of the enzyme. Enzyme Microb Technol. 2015;71:53–57.
   PubMed Web of Science ®Google Scholar
• Torres-Salas P, del Monte-Martinez A, Cutiño-Avila B, et al. Immobilized biocatalysts: novel approaches and tools for binding enzymes to supports. Adv Mater. 2011;23(44):5275–5282.
   PubMed Web of Science ®Google Scholar
• Subramanian A, Kennel SJ, Oden PI, et al. Comparison of techniques for enzyme immobilisation on silicon supports effect of cross-linker chain length on enzyme activity. Enzyme Microb Technol. 1999;24(1–2):26–34.
   Web of Science ®Google Scholar
• Long JC, Lee WC, Guo SH. Immobilization of cell-associated enzyme by entrapping in gluten matrix. Biocatal Biotransformation. 2000;17(6):431–443.
   Web of Science ®Google Scholar
• Shen Q, Yang R, Hua X, et al. Gelatin-templated biomimetic calcification for β-galactosidase immobilization. Process Biochem. 2011;46(8):1565–1571.
   Web of Science ®Google Scholar
• Long J, Zhang B, Li X, et al. Effective production of resistant starch using pullulanase immobilized onto magnetic chitosan/Fe3O4 nanoparticles. Food Chem. 2018;239:276–286.
   PubMed Web of Science ®Google Scholar
• Yan Y, Hao Y, Hu S, et al. Hollow fibre cell fishing with high performance liquid chromatography for screening bioactive anthraquinones from traditional Chinese medicines. J Chromatogr A. 2013;1322:8–17.
   PubMed Web of Science ®Google Scholar
• Tao Y, Zhang Y, Wang Y, et al. Hollow fiber based affinity selection combined with high performance liquid chromatography-mass spectroscopy for rapid screening lipase inhibitors from lotus leaf. Anal Chim Acta. 2013;785:75–81.
   PubMed Web of Science ®Google Scholar
• Gorecka E, Jastrzebska M. Immobilization techniques and biopolymer carriers. Food Sci Biotechnol. 2011;75:65–86.
   Google Scholar
• Sheldon RA. Cross-linked enzyme aggregates (CLEAs): stable and recyclable biocatalysts. Biochem Soc Trans. 2007;35(Pt 6):1583–1587.
   PubMedGoogle Scholar
• Wubshet SG, Liu BR, Kongstad KT, et al. Combined magnetic ligand fishing and high-resolution inhibition profiling for identification of α-glucosidase inhibitory ligands: a new screening approach based on complementary inhibition and affinity profiles. Talanta. 2019;200:279–287.
   PubMed Web of Science ®Google Scholar
• Souza RRD, Ferreira RDM, Tambourgi EB. Immobilization of lipase from Candida rugosa on hydrophobic and mesoporous support by adsorption (MCM 41). Chem Eng Trans. 2014;37:565–570.
   Google Scholar
• Asmat S, Husain Q, Khan M. Polypyrrole-methylanthranilate functionalized “worm-like” titanium dioxide nanocomposite as an innovative tool for immobilization of lipase: preparation, activity-stability and molecular docking investigations. New J Chem. 2018;42(1):91–102.
   Web of Science ®Google Scholar
• Tao Y, Chen Z, Zhang Y, et al. Immobilized magnetic beads based multi-target affinity selection coupled with high performance liquid chromatography-mass spectrometry for screening anti-diabetic compounds from a Chinese medicine "Tang-Zhi-Qing". J Pharm Biomed Anal. 2013;78–79:190–201.
   PubMed Web of Science ®Google Scholar
• Hamzah F, Saleh NH, Alimin N, et al. Performance of mixed cellulase-amylase immobilized on PSF membrane support via cross-linked with glutaraldehyde in enzymatic hydrolysis. AMR. 2015;1119:542–547.
   Google Scholar
• Zhang L, Shi JF, Jiang ZY, et al. Bioinspired preparation of polydopamine microcapsule for multienzyme system construction. Green Chem. 2011;13(2):300–306.
   Web of Science ®Google Scholar
• Renberg B, Sato K, Mawatari K, et al. Serial DNA immobilization in micro- and extended nanospace channels. Lab Chip. 2009;9(11):1517–1523.
   PubMed Web of Science ®Google Scholar
• Arora A, Simone G, Salieb-Beugelaar GB, et al. Latest developments in micro total analysis systems. Anal Chem. 2010;82(12):4830–4847.
   PubMed Web of Science ®Google Scholar
• Vong TH, Maat J, van Beek TA, et al. Site-specific immobilization of DNA in glass microchannels via photolithography. Langmuir. 2009;25(24):13952–13958.
   PubMed Web of Science ®Google Scholar
• Vong TH, Schoffelen S, van Dongen SFM, et al. A DNA-based strategy for dynamic positional enzyme immobilization inside fused silica microchannels. Chem Sci. 2011;2(7):1278–1285.
   Web of Science ®Google Scholar
• Bhattacharya A, Pletschke BI. Magnetic cross-linked enzyme aggregates (CLEAs): a novel concept towards carrier free immobilization of lignocellulolytic enzymes. Enzyme Microb Technol. 2014;61–62:17–27.
   PubMed Web of Science ®Google Scholar
• Cui JD, Zhang S, Sun LM. Cross-linked enzyme aggregates of phenylalanine ammonia lyase: novel biocatalysts for synthesis of L-phenylalanine. Appl Biochem Biotechnol. 2012;167(4):835–844.
   PubMed Web of Science ®Google Scholar
• Illanes A, Wilson L, Caballero E, et al. Crosslinked penicillin acylase aggregates for synthesis of β-lactam antibiotics in organic medium. ABAB. 2006;133(3):189–202.
   Google Scholar
• Cao L. Immobilised enzymes: science or art? Curr Opin Chem Biol. 2005;9(2):217–226.
   PubMed Web of Science ®Google Scholar
• Dalal S, Kapoor M, Gupta MN. Preparation and characterization of combi-CLEAs catalyzing multiple non-Cascade reactions. J Mol Catal B Enzym. 2007;44(3–4):128–132.
   Google Scholar
• Contesini FJ, Figueira JDA, Kawaguti HY, et al. Potential applications of carbohydrases immobilization in the food industry. Int J Mol Sci. 2013;14(1):1335–1369.
   PubMed Web of Science ®Google Scholar
• Gupta P, Dutt K, Misra S, et al. Characterization of cross-linked immobilized lipase from thermophilic mould Thermomyces lanuginosa using glutaraldehyde. Bioresour Technol. 2009;100(18):4074–4076.
   PubMed Web of Science ®Google Scholar
• Hormigo D, García-Hidalgo J, Acebal C, et al. Preparation and characterization of cross-linked enzyme aggregates (CLEAs) of recombinant poly-3-hydroxybutyrate depolymerase from streptomyces exfoliatus. Bioresour Technol. 2012;115:177–182.
   PubMed Web of Science ®Google Scholar
• Vaidya BK, Kuwar SS, Golegaonkar SB, et al. Preparation of crosslinked enzyme aggregates of L-aminoacylase via co-aggregation with polyethyleneimine. J Mol Catal B: Enzym. 2012;74(3–4):184–191.
   Google Scholar
• Barbosa O, Torres R, Ortiz C, et al. Versatility of glutaraldehyde to immobilize lipases: effect of the immobilization protocol on the properties of lipase B from Candida antarctica. Process Biochem. 2012;47(8):1220–1227.
   Web of Science ®Google Scholar
• Yildirim D, Tükel SS, Alagöz D. Crosslinked enzyme aggregates of hydroxynitrile lyase partially purified from Prunus dulcis seeds and its application for the synthesis of enantiopure cyanohydrins. Biotechnol Prog. 2014;30(4):818–827.
   PubMed Web of Science ®Google Scholar
• Bilal M, Cui J, Iqbal HMN. Tailoring enzyme microenvironment: state-of-the-art strategy to fulfill the quest for efficient bio-catalysis. Int J Biol Macromol. 2019;130:186–196.
   PubMed Web of Science ®Google Scholar
• Montoro-García S, Gil-Ortiz F, Navarro-Fernández J, et al. Improved cross-linked enzyme aggregates for the production of desacetyl beta-lactam antibiotics intermediates . Bioresour Technol. 2010;101(1):331–336.
   PubMed Web of Science ®Google Scholar
• Talekar S, Ghodake V, Ghotage T, et al. Novel magnetic cross-linked enzyme aggregates (magnetic CLEAs) of alpha amylase. Bioresour Technol. 2012;123:542–547.
   PubMed Web of Science ®Google Scholar
• Guajardo N, Ahumada K, de María PD. Immobilized lipase-CLEA aggregates encapsulated in lentikats® as robust biocatalysts for continuous processes in deep eutectic solvents. J Biotechnol. 2020;310:97–102.
   PubMed Web of Science ®Google Scholar
• Paitaid P, H-Kittikun A. Magnetic cross-linked enzyme aggregates of Aspergillus oryzae ST11 lipase using polyacrylonitrile coated magnetic nanoparticles for biodiesel production. Appl Biochem Biotechnol. 2020;190(4):1319–1332.
   PubMed Web of Science ®Google Scholar
• Mehdi WA, Mehde AA, Severgün O, et al. Lipase-based on starch material as a development matrix with magnetite cross-linked enzyme aggregates and its application. Int J Biol Macromol. 2018;120(Pt B):1533–1543.
   PubMedGoogle Scholar
• Khanahmadi S, Yusof F, Amid A, et al. Optimized preparation and characterization of CLEA-lipase from cocoa pod husk. J Biotechnol. 2015;202:153–161.
   PubMed Web of Science ®Google Scholar
• Fang SM, Wang HN, Zhao ZX, et al. Immobilized enzyme reactors in HPLC and its application in inhibitor screening: a review. J Pharm Anal. 2012;2(2):83–89.
   PubMedGoogle Scholar
• Vanzolini KL, Jiang Z, Zhang X, et al. Acetylcholinesterase immobilized capillary reactors coupled to protein coated magnetic beads: a new tool for plant extract ligand screening. Talanta. 2013;116:647–652.
   PubMed Web of Science ®Google Scholar
• Yu S, Xiao Q, Zhu B, et al. Gas chromatography-mass spectrometry determination of earthy-musty odorous compounds in waters by two phase hollow-fiber liquid-phase microextraction using polyvinylidene fluoride fibers. J Chromatogr A. 2014;1329:45–51.
   PubMed Web of Science ®Google Scholar
• Liu H, Lei M, Liang X, et al. Simultaneous determination of three purines in Alysicarpus vaginalis (L.) DC. by hollow fiber-based liquid-phase microextraction combined with high-performance liquid chromatography. Biomed Chromatogr. 2014;28(2):311–316.
   PubMed Web of Science ®Google Scholar
• Chen L, Wang X, Liu Y, et al. Dual-target screening of bioactive components from traditional Chinese medicines by hollow fiber-based ligand fishing combined with liquid chromatography-mass spectrometry. J Pharm Biomed Anal. 2017;143:269–276.
   PubMed Web of Science ®Google Scholar
• Zhang Y, Wang Q, Liu R, et al. Rapid screening and identification of monoamine oxidase-A inhibitors from Corydalis rhizome using enzyme-immobilized magnetic beads based method. J Chromatogr A. 2019;1592:1–8.
   PubMed Web of Science ®Google Scholar
• Arai MA, Kobatake E, Koyano T, et al. A method for the rapid discovery of naturally occurring products by proteins immobilized on magnetic beads and reverse affinity chromatography. Chem Asian J. 2009;4(12):1802–1808.
   PubMed Web of Science ®Google Scholar
• Tao Y, Zhang Y, Cheng Y, et al. Rapid screening and identification of α-glucosidase inhibitors from mulberry leaves using enzyme-immobilized magnetic beads coupled with HPLC/MS and NMR. Biomed Chromatogr. 2013;27(2):148–155.
   PubMed Web of Science ®Google Scholar
• Meng J, Li Q, Cao Z, et al. Rapid screening and separation of active compounds against α-amylase from Toona sinensis by ligand fishing and high-speed counter-current chromatography. Int J Biol Macromol. 2021;174:270–277.
   PubMed Web of Science ®Google Scholar
• Cheng G, Xing J, Pi Z, et al. α-Glucosidase immobilization on functionalized Fe3O4 magnetic nanoparticles for screening of enzyme inhibitors. Chin Chem Lett. 2019;30(3):656–659.
   Web of Science ®Google Scholar
• Wubshet SG, Brighente IMC, Moaddel R, et al. Magnetic ligand fishing as a targeting tool for HPLC-HRMS-SPE-NMR: α-glucosidase inhibitory ligands and alkylresorcinol glycosides from eugenia catharinae. J Nat Prod. 2015;78(11):2657–2665.
   PubMed Web of Science ®Google Scholar
• Zhang S, Wu D, Li H, et al. Rapid identification of α-glucosidase inhibitors from Dioscorea opposita Thunb peel extract by enzyme functionalized Fe3O4 magnetic nanoparticles coupled with HPLC-MS/MS. Food Funct. 2017;8(9):3219–3227.
   PubMed Web of Science ®Google Scholar
• Ma R, Weng H, Liang J. Screening of lipase inhibitors in Folium mori with lipase-linked magnetic microspheres by high-performance liquid chromatography and evaluation in diabetic mice. J Sep Sci. 2016;39(23):4474–4483.
   PubMed Web of Science ®Google Scholar
• Zhu Y, Ren X, Yuan L, et al. Fast identification of lipase inhibitors in oolong tea by using lipase functionalised Fe3O4 magnetic nanoparticles coupled with UPLC-MS/MS. Food Chem. 2015;173:521–526.
   PubMed Web of Science ®Google Scholar
• Petersen MJ, Lima RCL, Kjaerulff L, et al. Immobilized α-amylase magnetic beads for ligand fishing: proof of concept and identification of α-amylase inhibitors in Ginkgo biloba. Phytochemistry. 2019;164:94–101.
   PubMed Web of Science ®Google Scholar
• Kuang J, Chen L, Tang Q, et al. The role of Sirt6 in obesity and diabetes. Front Physiol. 2018;9:135.
   PubMed Web of Science ®Google Scholar
• Yasuda M, Wilson DR, Fugmann SD, et al. The synthesis and characterization of SIRT6 protein coated magnetic beads: identification of a novel inhibitor of SIRT6 deacetylase from medicinal plant extracts. Anal Chem. 2011;83(19):7400–7407.
   PubMed Web of Science ®Google Scholar
• Qing L, Tang N, Xue Y, et al. Identification of enzyme inhibitors using therapeutic target protein–magnetic nanoparticle conjugates. Anal Methods. 2012;4(6):1612–1615.
   PubMed Web of Science ®Google Scholar
• Nie Y, Wang WH. Immobilized enzyme reactor in on-line LC and its application in drug screening. Chroma. 2009;69(S1):5–12.
   Google Scholar
• Luckarift HR, Johnson GR, Spain JC. Silica-immobilized enzyme reactors; application to cholinesterase-inhibition studies. J Chromatogr B Analyt Technol Biomed Life Sci. 2006;843(2):310–316.
   PubMed Web of Science ®Google Scholar
• Lu J, Ye F, Zhang A, et al. Preparation and characterization of silica monolith modified with bovine serum albumin-gold nanoparticles conjugates and its use as chiral stationary phases for capillary electrochromatography. J Sep Sci. 2011;34(16–17):2329–2336.
   PubMed Web of Science ®Google Scholar
• Zhao S, Ji X, Lin P, et al. A gold nanoparticle-mediated enzyme bioreactor for inhibitor screening by capillary electrophoresis. Anal Biochem. 2011;411(1):88–93.
   PubMed Web of Science ®Google Scholar
• Grabar KC, Freeman RG, Hommer MB, et al. Preparation and characterization of Au colloid monolayers. Anal Chem. 1995;67(4):735–743.
   Web of Science ®Google Scholar
• Zhang A, Ye F, Lu J, et al. Screening α-glucosidase inhibitor from natural products by capillary electrophoresis with immobilised enzyme onto polymer monolith modified by gold nanoparticles. Food Chem. 2013;141(3):1854–1859.
   PubMed Web of Science ®Google Scholar
• Jiang T, Liang T, Wang Y, et al. Immobilized capillary tyrosinase microreactor for inhibitor screening in natural extracts by capillary electrophoresis. J Pharm Biomed Anal. 2013;84:36–40.
   PubMed Web of Science ®Google Scholar
• Liu DM, Chen J, Shi Y. An online immobilized α-glucosidase microreactor for enzyme kinetics and inhibition assays. RSC Adv. 2015;5(70):56841–56847.
   Web of Science ®Google Scholar
• Tang C, Liu Z, Qin N, et al. Novel cell membrane capillary chromatography for screening active compounds from natural products. Chromatographia. 2013;76(11–12):697–701.
   Web of Science ®Google Scholar
• Li Z, Ding Y, Li S, et al. Highly active, stable and self-antimicrobial enzyme catalysts prepared by biomimetic mineralization of copper hydroxysulfate. Nanoscale. 2016;8(40):17440–17445.
   PubMed Web of Science ®Google Scholar
• Zhou HC, Long JR, Yaghi OM. Introduction to metal-organic frameworks. Chem Rev. 2012;112(2):673–674.
   PubMed Web of Science ®Google Scholar
• Zhang X, Li G, Wu D, et al. Recent progress in the design fabrication of metal-organic frameworks-based nanozymes and their applications to sensing and cancer therapy. Biosens Bioelectron. 2019;137:178–198.
   PubMed Web of Science ®Google Scholar
• Fu LH, Qi C, Lin J, et al. Catalytic chemistry of glucose oxidase in cancer diagnosis and treatment. Chem Soc Rev. 2018;47(17):6454–6472.
   PubMed Web of Science ®Google Scholar
• Chen X, Xue S, Lin Y, et al. Immobilization of porcine pancreatic lipase onto a metal-organic framework, PPL@MOF: a new platform for efficient ligand discovery from natural herbs. Anal Chim Acta. 2020;1099:94–102.
   PubMed Web of Science ®Google Scholar
• Zhong Y, Yu L, He Q, et al. Bifunctional hybrid enzyme-catalytic metal organic framework reactors for α-glucosidase inhibitor screening. ACS Appl Mater Interfaces. 2019;11(36):32769–32777.
   PubMed Web of Science ®Google Scholar
• Deng S, Xia L, Xiao H. Screening of α-glucosidase inhibitors from green tea extracts using immobilized enzymes affinity capture combined with UHPLC-QTOF MS analysis. Chem Commun (Camb). 2014;50(20):2582–2584.
   PubMed Web of Science ®Google Scholar
• Qian K, Wang H, Liu J, et al. Synthesis of α-glycosidase hybrid nano-flower and application for enriching and screening α-glycosidase inhibitors. New J Chem. 2018;42(1):429–436.
   Web of Science ®Google Scholar
• Wang LB, Wang YC, He R, et al. A new nanobiocatalytic system based on allosteric effect with dramatically enhanced enzymatic performance. J Am Chem Soc. 2013;135(4):1272–1275.
   PubMed Web of Science ®Google Scholar
• Guo S, Lin X, Wang Y, et al. Fabrication of paper-based enzyme immobilized microarray by 3D-printing technique for screening α-glucosidase inhibitors in mulberry leaves and lotus leaves. Chin Med. 2019;14(13):13.
   PubMedGoogle Scholar
• Wang H, Zhao X, Wang S, et al. Fabrication of enzyme-immobilized halloysite nanotubes for affinity enrichment of lipase inhibitors from complex mixtures. J Chromatogr A. 2015;1392:20–27.
   PubMed Web of Science ®Google Scholar
• Yang S, Gao H. Nanoparticles for modulating tumor microenvironment to improve drug delivery and tumor therapy. Pharmacol Res. 2017;126:97–108.
   PubMed Web of Science ®Google Scholar
• Amreddy N, Babu A, Muralidharan R, et al. Recent advances in nanoparticle-based cancer drug and gene delivery. Adv Cancer Res. 2018;137:115–170.
   PubMed Web of Science ®Google Scholar
• Carpino PA, Goodwin B. Diabetes area participation analysis: a review of companies and targets described in the 2008–2010 patent literature. Expert Opin Ther Pat. 2010;20(12):1627–1651.
   PubMed Web of Science ®Google Scholar
• Ladet SG, Tahiri K, Montembault AS, et al. Multi-membrane chitosan hydrogels as chondrocytic cell bioreactors. Biomaterials. 2011;32(23):5354–5364.
   PubMed Web of Science ®Google Scholar
• Giese EC, Silva DDV, Costa AFM, et al. Immobilized microbial nanoparticles for biosorption. Crit Rev Biotechnol. 2020;40(5):653–666.
   PubMed Web of Science ®Google Scholar