CD171 Multi-epitope peptide design based on immuno-informatics approach as a cancer vaccine candidate for glioblastoma

References

• Abraham Peele, K., Srihansa, T., Krupanidhi, S., Ayyagari, V. S., & Venkateswarulu, T. C. (2021). Design of multi-epitope vaccine candidate against SARS-CoV-2: A in-silico study. Journal of Biomolecular Structure and Dynamics, 39(10), 3793–3801. https://doi.org/10.1080/07391102.2020.1770127
   PubMed Web of Science ®Google Scholar
• Ali, M., Pandey, R. K., Khatoon, N., Narula, A., Mishra, A., & Prajapati, V. K. (2017). Exploring dengue genome to construct a multi-epitope based subunit vaccine by utilizing immunoinformatics approach to battle against dengue infection. Scientific Reports, 7(1), 1–3. Aug 23 https://doi.org/10.1038/s41598-017-09199-w
   PubMed Web of Science ®Google Scholar
• Baldauf, K. J., Royal, J. M., Hamorsky, K. T., & Matoba, N. (2015). Cholera toxin B: One subunit with many pharmaceutical applications. Toxins, 7(3), 974–996. Marhttps://doi.org/10.3390/toxins7030974
   PubMed Web of Science ®Google Scholar
• Boo, Y. J., Park, J. M., Kim, J., Chae, Y. S., Min, B. W., Um, J. W., & Moon, H. Y. (2007). L1 expression as a marker for poor prognosis, tumor progression, and short survival in patients with colorectal cancer. Annals of Surgical Oncology, 14(5), 1703–1711. May https://doi.org/10.1245/s10434-006-9281-8
   PubMed Web of Science ®Google Scholar
• Buchan, D. W., Minneci, F., Nugent, T. C., Bryson, K., & Jones, D. T. (2013). Scalable web services for the PSIPRED Protein Analysis Workbench. Nucleic Acids Research, 41(Web Server issue), W349–57. Jul 1https://doi.org/10.1093/nar/gkt381
   PubMed Web of Science ®Google Scholar
• Bui, H. H., Sidney, J., Li, W., Fusseder, N., & Sette, A. (2007). Development of an epitope conservancy analysis tool to facilitate the design of epitope-based diagnostics and vaccines. BMC Bioinformatics, 8(1), 361–366. Dec https://doi.org/10.1186/1471-2105-8-361
   PubMed Web of Science ®Google Scholar
• Cao, Y., Li, D., Fu, Y., Bai, Q., Chen, Y., Bai, X., Jing, Z., Sun, P., Bao, H., Li, P., Zhang, J., Ma, X., Lu, Z., & Liu, Z. (2017). Rational design and efficacy of a multi-epitope recombinant protein vaccine against foot-and-mouth disease virus serotype A in pigs. Antiviral Research, 140, 133–141. Apr 1https://doi.org/10.1016/j.antiviral.2017.01.023
   PubMed Web of Science ®Google Scholar
• Davis, F. G., Kupelian, V., Freels, S., McCarthy, B., & Surawicz, T. (2001). Prevalence estimates for primary brain tumors in the United States by behavior and major histology groups. Neuro-oncology, 3(3), 152–158. https://doi.org/10.1093/neuonc/3.3.152
   PubMed Web of Science ®Google Scholar
• Dawood, R. M., Moustafa, R. I., Abdelhafez, T. H., El-Shenawy, R., El-Abd, Y., El Din, N. G., Dubuisson, J., & El Awady, M. K. (2019). A multiepitope peptide vaccine against HCV stimulates neutralizing humoral and persistent cellular responses in mice. BMC Infectious Diseases, 19(1), 1–1. Dec https://doi.org/10.1186/s12879-019-4571-5
   PubMed Web of Science ®Google Scholar
• Debiec, H., Christensen, E. I., & Ronco, P. M. (1998). The cell adhesion molecule L1 is developmentally regulated in the renal epithelium and is involved in kidney branching morphogenesis. The Journal of Cell Biology, 143(7), 2067–2079. Dec 28https://doi.org/10.1083/jcb.143.7.2067
   PubMed Web of Science ®Google Scholar
• DeLano, W. L. (2002). Pymol: An open-source molecular graphics tool. CCP4 Newsletter on Protein Crystallography, 40(1), 82–92.
   Google Scholar
• Dimitrov, I., Bangov, I., Flower, D. R., & Doytchinova, I. (2014). AllerTOP v. 2—a server for in silico prediction of allergens. Journal of Molecular Modeling, 20(6), 2278–2276. Jun https://doi.org/10.1007/s00894-014-2278-5
   PubMed Web of Science ®Google Scholar
• Dimitrov, I., Naneva, L., Doytchinova, I., & Bangov, I. (2014). AllergenFP: Allergenicity prediction by descriptor fingerprints. Bioinformatics (Oxford, England), 30(6), 846–851. Mar 15https://doi.org/10.1093/bioinformatics/btt619
   PubMed Web of Science ®Google Scholar
• Doytchinova, I. A., & Flower, D. R. (2002). Quantitative approaches to computational vaccinology. Immunology and Cell Biology, 80(3), 270–279. Junhttps://doi.org/10.1046/j.1440-1711.2002.01076.x
   PubMed Web of Science ®Google Scholar
• Doytchinova, I. A., & Flower, D. R. (2007). VaxiJen: A server for prediction of protective antigens, tumour antigens and subunit vaccines. BMC Bioinformatics, 8(1), 1–7. Dec https://doi.org/10.1186/1471-2105-8-4
   PubMedGoogle Scholar
• Flower, D. R., McSparron, H., Blythe, M. J., Zygouri, C., Taylor, D., Guan, P., Wan, S., Coveney, P., Walshe, V., Borrow, P., & Doytchinova, I. A. (1999). Computational vaccinology: Quantitative approaches. In Novartis Foundation Symposium, 2003 January 1 (pp. 102–125). John Wiley. https://doi.org/10.1002/0470090766
   Google Scholar
• Gasteiger, E., Hoogland, C., Gattiker, A., Wilkins, M. R., Appel, R. D., & Bairoch, A. (2005). Protein identification and analysis tools on the ExPASy server. In The proteomics protocols handbook (pp. 571–607). https://doi.org/10.1385/1-59259-890-0:571
   Google Scholar
• Gavert, N., Ben-Shmuel, A., Raveh, S., & Ben-Ze'ev, A. (2008). L1-CAM in cancerous tissues. Expert Opinion on Biological Therapy, 8(11), 1749–1757. Nov 157. https://doi.org/10.1517/14712598.8.11.1749
   PubMed Web of Science ®Google Scholar
• Geer, L. Y., Marchler-Bauer, A., Geer, R. C., Han, L., He, J., He, S., Liu, C., Shi, W., & Bryant, S. H. (2010). The NCBI biosystems database. Nucleic Acids Research, 38(Database issue), D492–6. Jan 1https://doi.org/10.1093/nar/gkp858
   PubMedGoogle Scholar
• Grote, A., Hiller, K., Scheer, M., Münch, R., Nörtemann, B., Hempel, D. C., & Jahn, D. (2005). JCat: A novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Research, 33(Web Server issue), W526–W531. https://doi.org/10.1093/nar/gki376
   PubMed Web of Science ®Google Scholar
• Guo, L., Yin, R., Liu, K., Lv, X., Li, Y., Duan, X., Chu, Y., Xi, T., & Xing, Y. (2014). Immunological features and efficacy of a multi-epitope vaccine CTB-UE against H. pylori in BALB/c mice model. Applied Microbiology and Biotechnology, 98(8), 3495–3507. Aprhttps://doi.org/10.1007/s00253-013-5408-6
   PubMed Web of Science ®Google Scholar
• Gupta, S., Kapoor, P., Chaudhary, K., Gautam, A., Kumar, R., & Raghava, G. P. (2013). In silico approach for predicting toxicity of peptides and proteins. PloS One, 8(9), e73957. https://doi.org/10.1371/journal.pone.0073957
   PubMed Web of Science ®Google Scholar
• Hanif, F., Muzaffar, K., Perveen, K., Malhi, S. M., & Simjee, S. U. (2017). Glioblastoma multiforme: A review of its epidemiology and pathogenesis through clinical presentation and treatment. Asian Pacific Journal of Cancer Prevention : APJCP, 18(1), 3–9. https://doi.org/10.22034/APJCP.2017.18.1.3
   PubMedGoogle Scholar
• Huszar, M., Moldenhauer, G., Gschwend, V., Ben-Arie, A., Altevogt, P., & Fogel, M. (2006). Expression profile analysis in multiple human tumors identifies L1 (CD171) as a molecular marker for differential diagnosis and targeted therapy. Human Pathology, 37(8), 1000–1008. 1https://doi.org/10.1016/j.humpath.2006.03.014
   PubMed Web of Science ®Google Scholar
• Jespersen, M. C., Peters, B., Nielsen, M., & Marcatili, P. (2017). BepiPred-2.0: Improving sequence-based B-cell epitope prediction using conformational epitopes. Nucleic Acids Research, 45(W1), W24–9. Jul 3https://doi.org/10.1093/nar/gkx346
   PubMed Web of Science ®Google Scholar
• Jiang, P., Cai, Y., Chen, J., Ye, X., Mao, S., Zhu, S., Xue, X., Chen, S., & Zhang, L. (2017). Evaluation of tandem Chlamydia trachomatis MOMP multi-epitopes vaccine in BALB/c mice model. Vaccine, 35(23), 3096–3103. May 25https://doi.org/10.1016/j.vaccine.2017.04.031
   PubMed Web of Science ®Google Scholar
• Kamthania, M., Srivastava, S., Desai, M., Jain, A., Shrivastav, A., & Sharma, D. K. (2019). Immunoinformatics Approach to design T-cell epitope-based vaccine against hendra virus. International Journal of Peptide Research and Therapeutics, 25(4), 1627–1637. Dec https://doi.org/10.1007/s10989-018-09805-z
   Web of Science ®Google Scholar
• Khatoon, N., Pandey, R. K., & Prajapati, V. K. (2017). Exploring Leishmania secretory proteins to design B and T cell multi-epitope subunit vaccine using immunoinformatics approach. Scientific Reports, 7(1), 1–2. Aug 15 https://doi.org/10.1038/s41598-017-08842-w
   PubMed Web of Science ®Google Scholar
• Kindy, M. S., Yu, J., Zhu, H., Smith, M. T., & Gattoni-Celli, S. (2016). A therapeutic cancer vaccine against GL261 murine glioma. Journal of Translational Medicine, 14, 1–9. Dec(https://doi.org/10.1186/s12967-015-0757-9
   PubMed Web of Science ®Google Scholar
• Kowitz, A., Kadmon, G., Verschueren, H., Remels, L., De Baetselier, P., Hubbe, M., Schachner, M., Schirrmacher, V., & Altevogt, P. (1993). Expression of L1 cell adhesion molecule is associated with lymphoma growth and metastasis. Clinical & Experimental Metastasis, 11(5), 419–429. Sep 1https://doi.org/10.1007/BF00132985
   PubMed Web of Science ®Google Scholar
• Kozakov, D., Hall, D. R., Xia, B., Porter, K. A., Padhorny, D., Yueh, C., Beglov, D., & Vajda, S. (2017). The ClusPro web server for protein–protein docking. Nature Protocols, 12(2), 255–278. Feb https://doi.org/10.1038/nprot.2016.169
   PubMed Web of Science ®Google Scholar
• Krogh, A., Larsson, B., Von Heijne, G., & Sonnhammer, E. L. (2001). Predicting transmembrane protein topology with a hidden Markov model: Application to complete genomes. Journal of Molecular Biology, 305(3), 567–580. Jan 19https://doi.org/10.1006/jmbi.2000.4315
   PubMed Web of Science ®Google Scholar
• Lear, S., & Cobb, S. L. (2016). Pep-Calc.com: A set of web utilities for the calculation of peptide and peptoid properties and automatic mass spectral peak assignment. Journal of Computer-Aided Molecular Design, 30(3), 271–277. Mar 1https://doi.org/10.1007/s10822-016-9902-7
   PubMed Web of Science ®Google Scholar
• Lennerz, V., Gross, S., Gallerani, E., Sessa, C., Mach, N., Boehm, S., Hess, D., von Boehmer, L., Knuth, A., Ochsenbein, A. F., Gnad-Vogt, U., Zieschang, J., Forssmann, U., Woelfel, T., & Kaempgen, E. (2014). Immunologic response to the survivin-derived multi-epitope vaccine EMD640744 in patients with advanced solid tumors. Cancer Immunology, Immunotherapy : CII, 63(4), 381–394. Apr 1https://doi.org/10.1007/s00262-013-1516-5
   PubMed Web of Science ®Google Scholar
• Li, X., Guo, L., Kong, M., Su, X., Yang, D., Zou, M., Liu, Y., & Lu, L. (2015). Design and evaluation of a multi-epitope peptide of human metapneumovirus. Intervirology, 58(6), 403–412. https://doi.org/10.1159/000445059
   PubMed Web of Science ®Google Scholar
• López-Blanco, J. R., Aliaga, J., Quintana-Ortí, E. S., & Chacón, P. (2014). iMODS: Internal coordinates normal mode analysis server. Nucleic Acids Research, 42(Web Server issue), W271–W276. doi: 10.1093/nar/gku339
   PubMed Web of Science ®Google Scholar
• Lovell, S. C., Davis, I. W., Arendall, I. I. W., De Bakker, P. I., Word, J. M., Prisant, M. G., Richardson, J. S., & Richardson, D. C. (2003). Structure validation by Cα geometry: ϕ, ψ and Cβ deviation. Proteins: Structure, Function, and Bioinformatics, 50(3), 437–450. https://doi.org/10.1002/prot.10286
   PubMed Web of Science ®Google Scholar
• Mahdevar, E., Safavi, A., Abiri, A., Kefayat, A., Hejazi, S. H., Miresmaeili, S. M., & Iranpur Mobarakeh, V. (2021). Iranpur Mobarakeh V. Exploring the cancer-testis antigen BORIS to design a novel multi-epitope vaccine against breast cancer based on immunoinformatics approaches. Journal of Biomolecular Structure and Dynamics, 1–8. Feb 18: https://doi.org/10.1080/07391102.2021.1883111
   Google Scholar
• Maness, P. F., & Schachner, M. (2007). Neural recognition molecules of the immunoglobulin superfamily: Signaling transducers of axon guidance and neuronal migration. Nature Neuroscience, 10(1), 19–26. https://doi.org/10.1038/nn1827
   PubMed Web of Science ®Google Scholar
• Meza, B., Ascencio, F., Sierra-Beltrán, A. P., Torres, J., & Angulo, C. (2017). A novel design of a multi-antigenic, multistage and multi-epitope vaccine against Helicobacter pylori: An in silico approach. Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, 49, 309–317. Apr 1https://doi.org/10.1016/j.meegid.2017.02.007
   PubMed Web of Science ®Google Scholar
• Moghri, S. A., Ranjbar, M., Hassannia, H., & Khakdan, F. (2021). Designing a novel multi-epitope vaccine against SARS-CoV-2; implication for viral binds and fusion inhibition through inducing neutralizing antibodies. bioRxiv, Jan 1. https://doi.org/10.1101/2021.06.16.448772
   Google Scholar
• Moise, L., Gutierrez, A., Kibria, F., Martin, R., Tassone, R., Liu, R., Terry, F., Martin, B., & De Groot, A. S. (2015). iVAX: An integrated toolkit for the selection and optimization of antigens and the design of epitope-driven vaccines. Human Vaccines & Immunotherapeutics, 11(9), 2312–2321. Sep 2https://doi.org/10.1080/21645515.2015.1061159
   PubMed Web of Science ®Google Scholar
• Morla, S., Makhija, A., & Kumar, S. (2016). Synonymous codon usage pattern in glycoprotein gene of rabies virus. Gene, 584(1), 1–6. Jun 10https://doi.org/10.1016/j.gene.2016.02.047
   PubMed Web of Science ®Google Scholar
• Nair, D. T., Singh, K., Siddiqui, Z., Nayak, B. P., Rao, K. V., & Salunke, D. M. (2002). Epitope recognition by diverse antibodies suggests conformational convergence in an antibody response. Journal of Immunology (Baltimore, Md. : 1950), 168(5), 2371–2382. Mar 1https://doi.org/10.4049/jimmunol.168.5.2371
   PubMed Web of Science ®Google Scholar
• Nezafat, N., Ghasemi, Y., Javadi, G., Khoshnoud, M. J., & Omidinia, E. (2014). A novel multi-epitope peptide vaccine against cancer: An in silico approach. Journal of Theoretical Biology, 349, 121–134. May 21https://doi.org/10.1016/j.jtbi.2014.01.018
   PubMed Web of Science ®Google Scholar
• Oh, T., Fakurnejad, S., Sayegh, E. T., Clark, A. J., Ivan, M. E., Sun, M. Z., Safaee, M., Bloch, O., James, C. D., & Parsa, A. T. (2014). Immunocompetent murine models for the study of glioblastoma immunotherapy. Journal of Translational Medicine, 12(1), 107–100. Dec https://doi.org/10.1186/1479-5876-12-107
   PubMedGoogle Scholar
• Oloomi, M., Javadi, M., Karam, M. R., Khezerloo, J. K., Haghri, Z., & Bouzari, S. (2020). Protective multi-epitope candidate vaccine for urinary tract infection. Biotechnology Reports, 28, e00564Dec 1https://doi.org/10.1016/j.btre.2020.e00564
   Google Scholar
• Pechriggl, E. J., Concin, N., Blumer, M. J., Bitsche, M., Zwierzina, M., Dudas, J., Koziel, K., Altevogt, P., Zeimet, A. G., & Fritsch, H. (2017). L1CAM in the early enteric and urogenital system. The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society, 65(1), 21–32. Janhttps://doi.org/10.1369/0022155416677241
   PubMed Web of Science ®Google Scholar
• Phongsisay, V., Iizasa, E. I., Hara, H., & Yoshida, H. (2015). Evidence for TLR4 and FcRγ-CARD9 activation by cholera toxin B subunit and its direct bindings to TREM2 and LMIR5 receptors. Molecular Immunology, 66(2), 463–471. Aug 1https://doi.org/10.1016/j.molimm.2015.05.008
   PubMed Web of Science ®Google Scholar
• Rapin, N., Lund, O., Bernaschi, M., & Castiglione, F. (2010). Computational immunology meets bioinformatics: The use of prediction tools for molecular binding in the simulation of the immune system. PLoS One, 5(4), e9862 10.1371/journal.pone.0009862
   PubMed Web of Science ®Google Scholar
• Riedle, S., Kiefel, H., Gast, D., Bondong, S., Wolterink, S., Gutwein, P., & Altevogt, P. (2009). Nuclear translocation and signalling of L1-CAM in human carcinoma cells requires ADAM10 and presenilin/gamma-secretase activity. The Biochemical Journal, 420(3), 391–402. Jun 15https://doi.org/10.1042/BJ20081625
   PubMed Web of Science ®Google Scholar
• Romeli, S., Hassan, S. S., & Yap, W. B. (2020). Multi-epitope peptide-based and vaccinia-based universal influenza vaccine candidates subjected to clinical trials. The Malaysian Journal of Medical Sciences : MJMS, 27(2), 10–20. Marhttps://doi.org/10.21315/mjms2020.27.2.2
   PubMed Web of Science ®Google Scholar
• Ropón-Palacios, G., Chenet-Zuta, M. E., Otazu, K., Olivos-Ramirez, G. E., & Camps, I. (2019). Novel multi-epitope protein containing conserved epitopes from different Leishmania species as potential vaccine candidate: Integrated immunoinformatics and molecular dynamics approach. Computational Biology and Chemistry, 83, 107157Dec 1https://doi.org/10.1016/j.compbiolchem.2019.107157
   PubMed Web of Science ®Google Scholar
• Roy, A., Kucukural, A., & Zhang, Y. (2010). I-TASSER: A unified platform for automated protein structure and function prediction. Nature Protocols, 5(4), 725–738. Aprhttps://doi.org/10.1038/nprot.2010.5
   PubMed Web of Science ®Google Scholar
• Safavi, A., Kefayat, A., Abiri, A., Mahdevar, E., Behnia, A. H., & Ghahremani, F. (2019). In silico analysis of transmembrane protein 31 (TMEM31) antigen to design novel multiepitope peptide and DNA cancer vaccines against melanoma. Molecular Immunology, 112, 93–102. Aug 1https://doi.org/10.1016/j.molimm.2019.04.030
   PubMed Web of Science ®Google Scholar
• Safavi, A., Kefayat, A., Mahdevar, E., Abiri, A., & Ghahremani, F. (2020). Exploring the out of sight antigens of SARS-CoV-2 to design a candidate multi-epitope vaccine by utilizing immunoinformatics approaches. Vaccine, 38(48), 7612–7628. Nov 10https://doi.org/10.1016/j.vaccine.2020.10.016
   PubMed Web of Science ®Google Scholar
• Safavi, A., Kefayat, A., Sotoodehnejadnematalahi, F., Salehi, M., & Modarressi, M. H. (2019). Production, purification, and in vivo evaluation of a novel multiepitope peptide vaccine consisted of immunodominant epitopes of SYCP1 and ACRBP antigens as a prophylactic melanoma vaccine. International Immunopharmacology, 76, 105872Nov 1https://doi.org/10.1016/j.intimp.2019.105872
   PubMed Web of Science ®Google Scholar
• Samad, A., Ahammad, F., Nain, Z., Alam, R., Imon, R. R., Hasan, M., & Rahman, M. S. (2020). Designing a multi-epitope vaccine against SARS-CoV-2: An immunoinformatics approach. Journal of Biomolecular Structure and Dynamics, 1–7. Jul 17: https://doi.org/10.1080/07391102.2020.1792347
   Google Scholar
• Schäfer, M. K., & Altevogt, P. (2010). L1CAM malfunction in the nervous system and human carcinomas. Cellular and Molecular Life Sciences : CMLS, 67(14), 2425–2437. Julhttps://doi.org/10.1007/s00018-010-0339-1
   PubMed Web of Science ®Google Scholar
• Siesser, P. F., & Maness, P. F. (2009). L1 cell adhesion molecules as regulators of tumor cell invasiveness. Cell Adhesion & Migration, 3(3), 275–277. Jul 1https://doi.org/10.4161/cam.3.3.8689
   PubMed Web of Science ®Google Scholar
• Skwarczynski, M., & Toth, I. (2014). Recent advances in peptide-based subunit nanovaccines. Nanomedicine (London, England), 9(17), 2657–2669. Dechttps://doi.org/10.2217/nnm.14.187
   PubMed Web of Science ®Google Scholar
• Slingluff, C. L., Lee, S., Zhao, F., Chianese-Bullock, K. A., Olson, W. C., Butterfield, L. H., Whiteside, T. L., Leming, P. D., & Kirkwood, J. M. (2013). A randomized phase II trial of multiepitope vaccination with melanoma peptides for cytotoxic T cells and helper T cells for patients with metastatic melanoma (E1602). Clinical Cancer Research : An Official Journal of the American Association for Cancer Research, 19(15), 4228–4238. Aug 1doi: 10.1158/1078-0432.
   PubMed Web of Science ®Google Scholar
• Tanaka, Y., Wada, H., Goto, R., Osada, T., Yamamura, K., Fukaya, S., Shimizu, A., Okubo, M., Minamiguchi, K., Ikizawa, K., Sasaki, E., & Utsugi, T. (2020). TAS0314, a novel multi-epitope long peptide vaccine, showed synergistic antitumor immunity with PD-1/PD-L1 blockade in HLA-A* 2402 mice. Scientific Reports, 10(1), 1–1. Oct 14 https://doi.org/10.1038/s41598-020-74187-6
   PubMed Web of Science ®Google Scholar
• Toledo, H., Baly, A., Castro, O., Resik, S., Laferté, J., Rolo, F., Navea, L., Lobaina, L., Cruz, O., Mı́guez, J., Serrano, T., Sierra, B., Pérez, L., Ricardo, M. E., Dubed, M., Lubián, A. L., Blanco, M., Millán, J. C., Ortega, A., … Duarte, C. A. (2001). A phase I clinical trial of a multi-epitope polypeptide TAB9 combined with Montanide ISA 720 adjuvant in non-HIV-1 infected human volunteers. Vaccine, 19(30), 4328–4336. Jul 20 https://doi.org/10.1016/S0264-410X(01)00111-6
   PubMed Web of Science ®Google Scholar
• Vajda, S., Yueh, C., Beglov, D., Bohnuud, T., Mottarella, S. E., Xia, B., Hall, D. R., & Kozakov, D. (2017). New additions to the ClusPro server motivated by CAPRI. Proteins, 85(3), 435–444. Marhttps://doi.org/10.1002/prot.25219
   PubMed Web of Science ®Google Scholar
• Wang, S., Peng, J., Ma, J., & Xu, J. (2016). Protein secondary structure prediction using deep convolutional neural fields. Scientific Reports, 6(1), 18962–18961. Jan 11 https://doi.org/10.1038/srep18962
   PubMedGoogle Scholar
• Wiederstein, M., & Sippl, M. J. (2007). ProSA-web: Interactive web service for the recognition of errors in three-dimensional structures of proteins. Nucleic Acids Research, 35(Web Server issue), W407–10. Jul 1https://doi.org/10.1093/nar/gkm290
   PubMed Web of Science ®Google Scholar
• Yang, M., Li, Y., Chilukuri, K., Brady, O. A., Boulos, M. I., Kappes, J. C., & Galileo, D. S. (2011). L1 stimulation of human glioma cell motility correlates with FAK activation. Journal of Neuro-Oncology, 105(1), 27–44. Octhttps://doi.org/10.1007/s11060-011-0557-x
   PubMed Web of Science ®Google Scholar
• Zhang, Q., Wang, P., Kim, Y., Haste-Andersen, P., Beaver, J., Bourne, P. E., Bui, H.-H., Buus, S., Frankild, S., Greenbaum, J., Lund, O., Lundegaard, C., Nielsen, M., Ponomarenko, J., Sette, A., Zhu, Z., & Peters, B. (2008). Immune epitope database analysis resource (IEDB-AR). Nucleic Acids Research, 36(Web Server issue), W513–8. May 31https://doi.org/10.1093/nar/gkn254
   PubMedGoogle Scholar
• Zhou, W. Y., Shi, Y., Wu, C., Zhang, W. J., Mao, X. H., Guo, G., Li, H. X., & Zou, Q. M. (2009). Therapeutic efficacy of a multi-epitope vaccine against Helicobacter pylori infection in BALB/c mice model. Vaccine, 27(36), 5013–5019. Aug 6https://doi.org/10.1016/j.vaccine.2009.05.009
   PubMed Web of Science ®Google Scholar