659
Views
6
CrossRef citations to date
0
Altmetric
Research Article

Detection of Fusobacterium Species in Human Feces Using Genus-Specific PCR Primers and Denaturing Gradient Gel Electrophoresis

, , &
Pages 129-132 | Published online: 11 Jul 2009

  • Atlas RM. In: Parks LC, ed. Microbiological Media. London, UK: CRC Press, 1993.
  • Ben Omar N, Ampe F. Microbial community dynamics during production of the Mexican fermented maize dough pozol. Appl Environ Microbiol 2000; 66: 3664-73.
  • Brosius J, Dull TJ, Sleeter DD, Noller HF. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coll. J Molec Biol 1981; 148: 107-27.
  • Collins MD, Lawson PA, Willems A, Cordoba JJ, FernandezGrarayzabal J, Garcia P, Cai J, Hippe J, Farrow JAE The phytogeny of the genus Clostridium : proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 1994; 44: 812-26.
  • Hofstad, T, 1992 The genus Fusobacterium, In: Balows et al. (ed.). The Prokaryotes Berlin, Germany: Springer, p. 41154126.
  • Ludwig, W, Strunk, O, 1997. ARB: a software environment for sequence data, http://www.biol.chemie.tu-muenchen.de/pub/ ARB/documentation/arb.ps.
  • Moore WE, Holdeman LV. Human fecal flora: the normal flora of 20 Japanese-Hawaiians. Appl Microbiol 1974; 27: 961-79.
  • Moore WE, Holdeman LV, Welley RW. Genus Fusobacterium. In: Krieg NR, Holt JG, eds. Bergey's Manual of Systematic Bacteriology, vol. 1. Baltimore, MD: Williams and Wilkins, 1989: 631.
  • Onderdonk AB. The intestinal microflora and intra-abdominal sepsis. In: Tannock GW, ed. Medical Importance of the Normal Microflora. London, UK: Kluwer Academic Publishers, 1999.
  • Salminen S, Bouley C, Boutron-Ruault M-C, Cummings JH, Franck A, Gibson GR, Isolauri E, Moreau M-C, Roberfroid M, Rowland I. Functional food science and gastrointestinal physiology and function. Br J Nutr 1998; 80: S147-71.
  • Suau A, Bonnet R, Sutren M, Godon J-J, Gibson GR, Collins MD, Dore J. Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl Environ Microbiol 1999; 65: 4799-807.
  • Suau, A, Rochet, V, Sghir, A, Gramet, G, Brewaeys, S, Sutren, M, Rigottier-Gois, L, Doré, J. Fusobacterium prausnitzii and related species represent a dominant group within the human fecal flora. Syst Appl Microbiol 2001; 24: 139-145.
  • Tannock GW. Normal Microflora. An Introduction to Microbes Inhabiting the Human Body. London, UK: Chapman and Hall, 1995.
  • Walter J, Hertel C, Tannock GW, Lis CM, Munro K, Hammes WP. Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis. Appl Environ Microbiol 2001; 67: 2578-85.
  • Walter J, Tannock GW, Tilsala-Timisjarvi A, Rodtong S, Loach DM, Munro K, Alatossava T. Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primer. Appl Environ Microbiol 2000; 66: 297-303.
  • Wang R-F, Cao W-W, Cemiglia CE. Phylogenetic analysis of Fusobacterium prausnitzii based upon the 16S rRNA gene sequence and PCR confirmation. Int J Syst Bacteriol 1996; 46: 341-3.
  • Wang R-F, Cao W-W, Cerniglia CE. PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples. Appl Environ Microbiol 1996; 62: 12427.
  • Wilson KH, Blitchington RB. Human colonie biota studied by ribosomal DNA sequence analysis. Appl Environ Microbiol 1996; 62: 2273-8.
  • Zoetendal EG, Akkermans ADL, de Vos WM. Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria. Appl Environ Microbiol 1998; 64: 3854-9.