OGT-induced O-GlcNAcylation of NEK7 protein aggravates osteoarthritis progression by enhancing NEK7/NLRP3 axis

References

• Global, regional, and national incidence, prevalence, and years lived with disability for 354 diseases and injuries for 195 countries and territories, 1990-2017: a systematic analysis for the global burden of disease study 2017. Lancet. 2018;392(10159):1–10.
   PubMed Web of Science ®Google Scholar
• Hunter DJ, Bierma-Zeinstra S. Osteoarthritis. Lancet. 2019;393(10182):1745–1759.
   PubMed Web of Science ®Google Scholar
• Blanco FJ, Guitian RN, Vazquez-Martul E, et al. Osteoarthritis chondrocytes die by apoptosis. A possible pathway for osteoarthritis pathology. Arthritis Rheum. 1998;41(2):284–289.
   PubMed Web of Science ®Google Scholar
• Thomas CM, Fuller CJ, Whittles CE, et al. Chondrocyte death by apoptosis is associated with cartilage matrix degradation. Osteoarthritis Cartilage. 2007;15(1):27–34.
   PubMed Web of Science ®Google Scholar
• Chang JK, Chang LH, Hung SH, et al. Parathyroid hormone 1-34 inhibits terminal differentiation of human articular chondrocytes and osteoarthritis progression in rats. Arthritis Rheum. 2009;60(10):3049–3060.
   PubMed Web of Science ®Google Scholar
• Yan JY, Tian FM, Wang WY, et al. Parathyroid hormone (1-34) prevents cartilage degradation and preserves subchondral bone micro-architecture in Guinea pigs with spontaneous osteoarthritis. Osteoarthritis Cartilage. 2014;22(11):1869–1877.
   PubMed Web of Science ®Google Scholar
• Sampson ER, Hilton MJ, Tian Y, et al. Teriparatide as a chondroregenerative therapy for injury-induced osteoarthritis. Sci Transl Med. 2011;3(101):101ra93.
   PubMed Web of Science ®Google Scholar
• Yang J, Hu S, Bian Y, et al. Targeting cell death: pyroptosis, ferroptosis, apoptosis and necroptosis in osteoarthritis. Front Cell Dev Biol. 2021;9:789948.
   PubMed Web of Science ®Google Scholar
• Chang X, Kang Y, Yang Y, et al. Pyroptosis: a novel intervention target in the progression of osteoarthritis. J Inflamm Res. 2022;15:3859–3871.
   PubMed Web of Science ®Google Scholar
• Yang X, Qian K. Protein O-GlcNAcylation: emerging mechanisms and functions. Nat Rev Mol Cell Biol. 2017;18(7):452–465.
   PubMed Web of Science ®Google Scholar
• Chatham JC, Zhang J, Wende AR. Role of O-linked N-Acetylglucosamine protein modification in cellular (patho)physiology. Physiol Rev. 2021;101(2):427–493.
   PubMed Web of Science ®Google Scholar
• Gloster TM, Vocadlo DJ. Mechanism, structure, and inhibition of O-GlcNAc processing enzymes. Curr Signal Transduct Ther. 2010;5(1):74–91.
   PubMed Web of Science ®Google Scholar
• Bond MR, Hanover JA. O-GlcNAc cycling: a link between metabolism and chronic disease. Annu Rev Nutr. 2013;33(1):205–229.
   PubMedGoogle Scholar
• Koyama T, Kamemura K. Global increase in O-linked N-acetylglucosamine modification promotes osteoblast differentiation. Exp Cell Res. 2015;338(2):194–202.
   PubMed Web of Science ®Google Scholar
• Tardio L, Andrés-Bergós J, Zachara NE, et al. O-linked N-acetylglucosamine (O-GlcNAc) protein modification is increased in the cartilage of patients with knee osteoarthritis. Osteoarthritis Cartilage. 2014;22(2):259–263.
   PubMed Web of Science ®Google Scholar
• Someya A, Ikegami T, Sakamoto K, et al. Glucosamine downregulates the IL-1beta-Induced expression of proinflammatory cytokine genes in human synovial MH7A cells by O-GlcNAc modification-Dependent and -Independent mechanisms. PLoS One. 2016;11(10):e165158.
   PubMed Web of Science ®Google Scholar
• Hu S, Zhang C, Ni L, et al. Stabilization of HIF-1alpha alleviates osteoarthritis via enhancing mitophagy. Cell Death Dis. 2020;11(6):481.
   PubMed Web of Science ®Google Scholar
• Glasson SS, Askew R, Sheppard B, et al. Deletion of active ADAMTS5 prevents cartilage degradation in a murine model of osteoarthritis. Nature. 2005;434(7033):644–648.
   PubMed Web of Science ®Google Scholar
• Yan Z, Da Q, Li Z, et al. Inhibition of NEK7 suppressed hepatocellular carcinoma progression by mediating cancer cell pyroptosis. Front Oncol. 2022;12:812655.
   PubMed Web of Science ®Google Scholar
• Boal-Carvalho I, Mazel-Sanchez B, Silva F, et al. Influenza a viruses limit NLRP3-NEK7-complex formation and pyroptosis in human macrophages. EMBO Rep. 2020;21(12):e50421.
   PubMed Web of Science ®Google Scholar
• Chen X, Liu G, Yuan Y, et al. NEK7 interacts with NLRP3 to modulate the pyroptosis in inflammatory bowel disease via NF-kappaB signaling. Cell Death Dis. 2019;10(12):906.
   PubMedGoogle Scholar
• Cai H, Wang P, Zhang B, et al. Expression of the NEK7/NLRP3 inflammasome pathway in patients with diabetic lower extremity arterial disease. BMJ Open Diabetes Res Care. 2020;8(2):e001808.
   PubMed Web of Science ®Google Scholar
• Li X, Gong W, Wang H, et al. O-GlcNAc transferase suppresses inflammation and necroptosis by targeting Receptor-Interacting serine/Threonine-Protein kinase 3. Immunity. 2019;50(3):576–590.e6.
   PubMed Web of Science ®Google Scholar
• Shi Q, Shen Q, Liu Y, et al. Increased glucose metabolism in TAMs fuels O-GlcNAcylation of lysosomal cathepsin B to promote cancer metastasis and chemoresistance. Cancer Cell. 2022;40(10):1207–1222.e10.
   PubMed Web of Science ®Google Scholar
• Raab S, Gadault A, Very N, et al. Dual regulation of fatty acid synthase (FASN) expression by O-GlcNAc transferase (OGT) and mTOR pathway in proliferating liver cancer cells. Cell Mol Life Sci. 2021;78(13):5397–5413.
   PubMed Web of Science ®Google Scholar
• Li YN, Chen CW, Trinh-Minh T, et al. Dynamic changes in O-GlcNAcylation regulate osteoclast differentiation and bone loss via nucleoporin 153. Bone Res. 2022;10(1):51.
   PubMed Web of Science ®Google Scholar
• Kim MJ, Kim HS, Lee S, et al. Hexosamine biosynthetic Pathway-Derived O-GlcNAcylation is critical for RANKL-Mediated osteoclast differentiation. Int J Mol Sci. 2021;22(16):8888.
   Web of Science ®Google Scholar
• Huang R, Hui Z, Wei S, et al. IRE1 signaling regulates chondrocyte apoptosis and death fate in the osteoarthritis. J Cell Physiol. 2022;237(1):118–127.
   PubMed Web of Science ®Google Scholar
• Ding Y, Wang L, Zhao Q, et al. MicroRNA‑93 inhibits chondrocyte apoptosis and inflammation in osteoarthritis by targeting the TLR4/NF‑kappaB signaling pathway. Int J Mol Med. 2019;43(2):779–790.
   PubMed Web of Science ®Google Scholar
• Bao J, Chen Z, Xu L, et al. Rapamycin protects chondrocytes against IL-18-induced apoptosis and ameliorates rat osteoarthritis. Aging (Albany NY). 2020;12(6):5152–5167.
   PubMedGoogle Scholar
• Zhang H, Zheng W, Li D, et al. miR-146a-5p promotes chondrocyte apoptosis and inhibits autophagy of osteoarthritis by targeting NUMB. Cartilage. 2021;13(2_suppl):1467S–1477S.
   PubMed Web of Science ®Google Scholar
• Zu Y, Mu Y, Li Q, et al. Icariin alleviates osteoarthritis by inhibiting NLRP3-mediated pyroptosis. J Orthop Surg Res. 2019;14(1):307.
   PubMed Web of Science ®Google Scholar
• Tonomura H, Takahashi KA, Mazda O, et al. Glutamine protects articular chondrocytes from heat stress and NO-induced apoptosis with HSP70 expression. Osteoarthritis Cartilage. 2006;14(6):545–553.
   PubMed Web of Science ®Google Scholar
• Wojdasiewicz P, Poniatowski LA, Szukiewicz D. The role of inflammatory and anti-inflammatory cytokines in the pathogenesis of osteoarthritis. Mediators Inflamm. 2014;2014:561459–561419.
   PubMed Web of Science ®Google Scholar
• Zhang L, Xing R, Huang Z, et al. Inhibition of synovial macrophage pyroptosis alleviates synovitis and fibrosis in knee osteoarthritis. Mediators Inflamm. 2019;2019:2165918–2165911.
   PubMed Web of Science ®Google Scholar
• Qian J, Fu P, Li S, et al. miR-107 affects cartilage matrix degradation in the pathogenesis of knee osteoarthritis by regulating caspase-1. J Orthop Surg Res. 2021;16(1):40.
   PubMed Web of Science ®Google Scholar
• Rozi R, Zhou Y, Rong K, et al. miR-124-3p sabotages lncRNA MALAT1 stability to repress chondrocyte pyroptosis and relieve cartilage injury in osteoarthritis. J Orthop Surg Res. 2022;17(1):453.
   PubMed Web of Science ®Google Scholar
• Schmid-Burgk JL, Chauhan D, Schmidt T, et al. A genome-wide CRISPR (clustered regularly interspaced short palindromic repeats) screen identifies NEK7 as an essential component of NLRP3 inflammasome activation. J Biol Chem. 2016;291(1):103–109.
   PubMed Web of Science ®Google Scholar
• Shi H, Wang Y, Li X, et al. NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component. Nat Immunol. 2016;17(3):250–258.
   PubMed Web of Science ®Google Scholar
• Yan W, Cao M, Ruan X, et al. Cancer-cell-secreted miR-122 suppresses O-GlcNAcylation to promote skeletal muscle proteolysis. Nat Cell Biol. 2022;24(5):793–804.
   PubMed Web of Science ®Google Scholar
• Hooftman A, Angiari S, Hester S, et al. The immunomodulatory metabolite itaconate modifies NLRP3 and inhibits inflammasome activation. Cell Metab. 2020;32(3):468–478.e7.
   PubMed Web of Science ®Google Scholar