Generation of thermostable Moloney murine leukemia virus reverse transcriptase variants using site saturation mutagenesis library and cell-free protein expression system

References

• Kimmel AR, Berger SL. Preparation of cDNA and the generation of cDNA libraries: overview. Methods Enzymol. 1987;152:307–316.10.1016/0076-6879(87)52035-3
   PubMed Web of Science ®Google Scholar
• Roberts JD, Bebenek K, Kunkel TA. The accuracy of reverse transcriptase from HIV-1. Science. 1988;242:1171–1173.10.1126/science.2460925
   PubMed Web of Science ®Google Scholar
• Patel PH, Jacobo-Molina A, Ding J, et al. Insights into DNA polymerization mechanisms from structure and function analysis of HIV-1 reverse transcriptase. Biochemistry. 1995;34:5351–5363.10.1021/bi00016a006
   PubMed Web of Science ®Google Scholar
• Georgiadis MM, Jessen SM, Ogata CM, et al. Mechanistic implications from the structure of a catalytic fragment of Moloney murine leukemia virus reverse transcriptase. Structure. 1995;3:879–892.10.1016/S0969-2126(01)00223-4
   PubMed Web of Science ®Google Scholar
• Das D, Georgiadis MM. The crystal structure of the monomeric reverse transcriptase from Moloney murine leukemia virus. Structure. 2004;12:819–829.10.1016/j.str.2004.02.032
   PubMed Web of Science ®Google Scholar
• Lim D, Gregorio GG, Bingman C, et al. Crystal structure of the Moloney murine leukemia virus RNaseH domain. J Virol. 2006;80:8379–8389.10.1128/JVI.00750-06
   PubMed Web of Science ®Google Scholar
• Cote ML, Roth MJ. Murine leukemia virus reverse transcriptase: structural comparison with HIV-1 reverse transcriptase. Virus Res. 2008;134:186–202.10.1016/j.virusres.2008.01.001
   PubMed Web of Science ®Google Scholar
• Yasukawa K, Nemoto D, Inouye K. Comparison of the thermal stabilities of reverse transcriptases from avian myeloblastosis virus and Moloney murine leukaemia virus. J Biochem. 2008;143:261–268.10.1093/jb/mvm217
   PubMed Web of Science ®Google Scholar
• Kotewicz ML, D’Alessio JM, Driftmier KM, et al. Cloning and overexpression of Moloney murine leukemia virus reverse transcriptase in Escherichia coli. Gene. 1985;35:249–258.10.1016/0378-1119(85)90003-4
   PubMed Web of Science ®Google Scholar
• Gerard GF, Potter RJ, Smith MD, et al. The role of template-primer in protection of reverse transcriptase from thermal inactivation. Nucleic Acids Res. 2002;30:3118–3129.10.1093/nar/gkf417
   PubMed Web of Science ®Google Scholar
• Mizuno M, Yasukawa K, Inouye K. Insight into the mechanism of the stabilization of Moloney murine leukaemia virus reverse transcriptase by eliminating RNase H activity. Biosci Biotechnol Biochem. 2010;74:440–442.10.1271/bbb.90777
   PubMed Web of Science ®Google Scholar
• Yasukawa K, Mizuno M, Konishi A, et al. Increase in thermal stability of Moloney murine leukaemia virus reverse transcriptase by site-directed mutagenesis. J Biotechnol. 2010;150:299–306.10.1016/j.jbiotec.2010.09.961
   PubMed Web of Science ®Google Scholar
• Konishi A, Ma X, Yasukawa K. Stabilization of Moloney murine leukemia virus reverse transcriptase by site-directed mutagenesis of the surface residue Val433. Biosci Biotechnol Biochem. 2014;78:147–150.
   PubMed Web of Science ®Google Scholar
• Baba M, Kakue R, Leucht C, et al. Further increase in thermostability of Moloney murine leukemia virus reverse transcriptase by mutational combination. Protein Eng Des Sel. 2017;30:551–557.
   PubMed Web of Science ®Google Scholar
• Arezi B, Hogrefe H. Novel mutations in Moloney murine leukemia virus reverse transcriptase increase thermostability through tighter binding to template-primer. Nucleic Acids Res. 2009;37:473–481.10.1093/nar/gkn952
   PubMed Web of Science ®Google Scholar
• Baranauskas A, Paliksa S, Alzbutas G, et al. Generation and characterization of new highly thermostable and processive M-MuLV reverse transcriptase variants. Protein Eng Des Sel. 2012;25:657–668.10.1093/protein/gzs034
   PubMed Web of Science ®Google Scholar
• Reetz MT, Carballeira JD. Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes. Nat Protoc. 2007;2:891–903.10.1038/nprot.2007.72
   PubMed Web of Science ®Google Scholar
• Reetz MT, Prasad S, Carballeira JD, et al. Iterative saturation mutagenesis accelerates laboratory evolution of enzyme stereoselectivity: rigorous comparison with traditional methods. J Am Chem Soc. 2010;132:9144–9152.10.1021/ja1030479
   PubMed Web of Science ®Google Scholar
• Cheng F, Xu J-M, Xiang C, et al. Simple-MSSM: a simple and efficient method for simultaneous multi-site saturation mutagenesis. Biotechnol Lett. 2017;39:567–575.10.1007/s10529-016-2278-x
   PubMed Web of Science ®Google Scholar
• Hodgman CE, Jewett MC. Cell-free synthetic biology: thinking outside the cell. Metab Eng. 2012;14:261–269.10.1016/j.ymben.2011.09.002
   PubMed Web of Science ®Google Scholar
• Whittaker JW. Cell-free protein synthesis: the state of the art. Biotechnol Lett. 2013;35:143–152.10.1007/s10529-012-1075-4
   PubMed Web of Science ®Google Scholar
• Katano Y, Hisayoshi T, Kuze I, et al. Expression of Moloney murine leukemia virus reverse transcriptase in a cell-free protein expression system. Biotechnol Lett. 2016;38:1203–1211.10.1007/s10529-016-2097-0
   PubMed Web of Science ®Google Scholar
• Kanno T, Tozawa Y. Protein engineering accelerated by cell-free technology. Methods Mol Biol. 2010;607:85–99.10.1007/978-1-60327-331-2
   PubMedGoogle Scholar
• Yasukawa K, Agata N, Inouye K. Detection of cesA mRNA from Bacillus cereus by RNA-specific amplification. Enzyme Microbiol Technol. 2010;46:391–396.10.1016/j.enzmictec.2009.12.009
   Web of Science ®Google Scholar
• Konishi A, Shinomura M, Yasukawa K. Enzymatic characterization of human immunodeficiency virus type 1 reverse transcriptase for use in cDNA synthesis. Appl Biochem Biotechnol. 2013;169:77–87.10.1007/s12010-012-9953-8
   PubMed Web of Science ®Google Scholar
• Acevedo-Rocha CG, Reetz MT, Nov Y. Economical analysis of saturation mutagenesis experiments. Sci Rep. 2015;5:10654.10.1038/srep10654
   PubMed Web of Science ®Google Scholar
• Zhao J, Frauenkron-Machedjou VJ, Kardashliev T, et al. Amino acid substitutions in random mutagenesis libraries: lessons from analyzing 3000 mutations. Appl Microbiol Biotechnol. 2017;101:3177–3187.10.1007/s00253-016-8035-1
   PubMed Web of Science ®Google Scholar
• Yasukawa K, Mizuno M, Inouye K. Characterization of Moloney murine leukaemia virus/avian myeloblastosis virus chimeric reverse transcriptases. J Biochem. 2009;145:315–324.10.1093/jb/mvn166
   PubMed Web of Science ®Google Scholar