PiggyBac-based screening identified BEM4 as a suppressor to rescue growth defects in och1-disrupted yeast cells

References

• Lagasse HA, Alexaki A, Simhadri VL, et al. Recent advances in (therapeutic protein) drug development. F1000Res. 2017;6:113.
   PubMedGoogle Scholar
• Jenkins N, Murphy L, Tyther R. Post-translational modifications of recombinant proteins: significance for biopharmaceuticals. Mol Biotechnol. 2008 Jun;39:113–118.
   PubMed Web of Science ®Google Scholar
• Pandhal J, PC W. N-linked glycoengineering for human therapeutic proteins in bacteria. Biotechnol Lett. 2010 Sep;32:1189–1198.
   PubMed Web of Science ®Google Scholar
• Helenius A, Aebi M. Roles of N-linked glycans in the endoplasmic reticulum. Annu Rev Biochem. 2004;73:1019–1049.
   PubMed Web of Science ®Google Scholar
• Tannous A, Pisoni GB, Hebert DN, et al. N-Linked Sugar-Regulated Protein Folding and Quality Control in the ER Semin Cell Dev Biol. 2015 May;41:79–89.
   PubMed Web of Science ®Google Scholar
• Sethuraman N, Stadheim TA. Challenges in therapeutic glycoprotein production . Curr Opin Biotechnol. 2006 Aug;17:341–346.
   PubMed Web of Science ®Google Scholar
• Kim JY, Kim Y-G, Lee GM. CHO cells in biotechnology for production of recombinant proteins: current state and further potential. Appl Microbiol Biotechnol. 2012 Feb;93:917–930.
   PubMed Web of Science ®Google Scholar
• Walsh G. Biopharmaceutical benchmarks 2014. Nat Biotechnol. 2014 Oct;32:992–1000.
   PubMed Web of Science ®Google Scholar
• Chiba Y, Suzuki M, Yoshida S, et al. Production of human compatible high mannose-type (Man5GlcNAc2) sugar chains in Saccharomyces cerevisiae. J Biol Chem. 1998 Oct;9(273):26298–26304.
   Web of Science ®Google Scholar
• Cregg JM, Cereghino JL, Shi J, et al. Recombinant protein expression in Pichia pastoris . Mol Biotechnol. 2000;16:23–52.
   PubMed Web of Science ®Google Scholar
• Chiba Y, Sakuraba H, Kotani M, et al Production in yeast of alpha-galactosidase A, a lysosomal enzyme applicable to enzyme replacement therapy for Fabry disease. Glycobiology. 2002;12:821–828.
   PubMed Web of Science ®Google Scholar
• Hamilton SR, Bobrowicz P, Bobrowicz B, et al Production of complex human glycoproteins in yeast. Science. 2003 Aug;301(5637):1244–1246.
   PubMed Web of Science ®Google Scholar
• Kuroda K, Kobayashi K, Tsumura H, et al. Production of Man5GlcNAc2-type sugar chain by the methylotrophic yeast Ogataea minuta. FEMS Yeast Res. 2006;6:1052–1062.
   PubMed Web of Science ®Google Scholar
• Ohashi T, Nakakita S, Sumiyoshi W, et al. Production of heterologous glycoproteins by a glycosylation-defective alg3och1 mutant of Schizosaccharomyces pombe. J Biotechnol. 2010;150:348–356.
   PubMed Web of Science ®Google Scholar
• Wildt S, Gerngross TU. The humanization of N-glycosylation pathways in yeast. Nat Rev Microbiol. 2005 Feb;3:119–128.
   PubMed Web of Science ®Google Scholar
• Jacobs PP, Geysens S, Vervecken W, et al Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology. Nat Protoc. 2009;4:58–70.
   PubMed Web of Science ®Google Scholar
• Jigami Y. Yeast glycobiology and its application. Biosci Biotechnol Biochem. 2008 Mar;72:637–648.
   PubMed Web of Science ®Google Scholar
• Nakayama K, Nagasu T, Shimma Y, et al. OCH1 encodes a novel membrane bound mannosyltransferase: outer chain elongation of asparagine-linked oligosaccharides. EMBO J. 1992;11:2511–2519.
   PubMed Web of Science ®Google Scholar
• Jacobs PP, Callewaert N. N-glycosylation engineering of biopharmaceutical expression systems. Curr Mol Med. 2009 Sep;9:774–800.
   PubMed Web of Science ®Google Scholar
• Nagasu T, Shimma Y, Nakanishi Y, et al. Isolation of new temperature-sensitive mutants of Saccharomyces cerevisiae deficient in mannose outer chain elongation. Yeast. 1992;8:535–547.
   PubMed Web of Science ®Google Scholar
• Abe H, Takaoka Y, Chiba Y, et al. Development of valuable yeast strains using a novel mutagenesis technique for the effective production of therapeutic glycoproteins. Glycobiology. 2009;19:428–436.
   PubMed Web of Science ®Google Scholar
• Xu S, Zhang G-Y, Zhang H, et al. Effects of Rho1, a small GTPase on the production of recombinant glycoproteins in Saccharomyces cerevisiae. Microb Cell Fact. 2016 Oct 21;15:179.
   PubMed Web of Science ®Google Scholar
• Levin DE. Regulation of cell wall biogenesis in Saccharomyces cerevisiae: the cell wall integrity signaling pathway. Genetics. 2011 Dec;189:1145–1175.
   PubMed Web of Science ®Google Scholar
• Abe H, Fujita Y, Chiba Y, et al. Upregulation of genes involved in gluconeogenesis and the glyoxylate cycle suppressed the drug sensitivity of an N-glycan-deficient Saccharomyces cerevisiae . Mutant Biosci Biotechnol Biochem. 2009;73:1398–1403.
   PubMed Web of Science ®Google Scholar
• Cary LC, Goebel M, Corsaro BG, et al. Transposon mutagenesis of baculoviruses: analysis of Trichoplusia ni transposon IFP2 insertions within the FP-locus of nuclear polyhedrosis viruses. Virology. 1989;172:156–169.
   PubMed Web of Science ®Google Scholar
• Fraser MJ, Cary L, Boonvisudhi K, et al Assay for movement of lepidopteran transposon IFP2 in insect cells using a baculovirus genome as a target DNA. Virology. 1995 Aug;20(2):397–407.
   Web of Science ®Google Scholar
• Thibault ST, Singer MA, Miyazaki WY, et al. A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. Nat Genet. 2004;36:283–287.
   PubMed Web of Science ®Google Scholar
• Yusa K. piggyBac Transposon. Microbiol Spectr. Apr 32015;3. MDNA3–0028–2014.
   PubMed Web of Science ®Google Scholar
• Longtine MS, McKenzie A 3rd, Demarini DJ, et al. Additional modules for versatile and economical PCR-based gene deletion and modification in saccharomyces cerevisiae. Yeast. 1998;14:953–961.
   PubMed Web of Science ®Google Scholar
• Taxis C, Knop M. System of centromeric, episomal, and integrative vectors based on drug resistance markers for Saccharomyces cerevisiae. Biotechniques. 2006 Jan;40:73–78.
   PubMed Web of Science ®Google Scholar
• Mohamed LA, Tachikawa H, Gao XD, et al. Yeast cell-based analysis of human lactate dehydrogenase isoforms. J Biochem. Dec 2015;158:467–476.
   PubMed Web of Science ®Google Scholar
• Yusa K, Zhou L, Li MA, et al A hyperactive piggyBac transposase for mammalian applications. Proc Natl Acad Sci. 2011 Jan;25(108):1531–1536.
   Web of Science ®Google Scholar
• Yusa K, Rashid ST, Strick-Marchand H, et al Targeted gene correction of alpha1-antitrypsin deficiency in induced pluripotent. Stem Cells Nature. 2011 Oct;12(478):391–394.
   Google Scholar
• Fujita M, Yoko OT, Jigami Y. Inositol deacylation by Bst1p is required for the quality control of glycosylphosphatidylinositol-anchored proteins. Mol Biol Cell. 2006 Feb;17:834–850.
   PubMed Web of Science ®Google Scholar
• Liu YS, Guo XY, Hirata T, et al. N-Glycan-dependent protein folding and endoplasmic reticulum retention regulate GPI-anchor processing. J Cell Biol. 2018 Feb;5(217):585–599.
   Web of Science ®Google Scholar
• Matabaro E, He Z, Liu YS, et al Molecular switching system using glycosylphosphatidylinositol to select cells highly expressing recombinant proteins. Sci Rep. 2017 Jun;22(7):4033.
   Web of Science ®Google Scholar
• Rong Y, Nakamura S, Hirata T, et al Genome-wide screening of genes required for glycosylphosphatidylinositol biosynthesis. PLoS One. 2015;10(e0138553).
   Google Scholar
• Sekiya-Kawasaki M, Abe M, Saka A, et al. Dissection of upstream regulatory components of the Rho1p effector, 1,3-beta-glucan synthase, in Saccharomyces cerevisiae Genetics.Oct 2002 ;162:663–676.
   PubMed Web of Science ®Google Scholar
• Mitra R, Fain-Thornton J, Craig NL. piggyBac can bypass DNA synthesis during cut and paste transposition. EMBO J. 2008 Apr;09(27):1097–1109.
   Web of Science ®Google Scholar
• Li X, Burnight ER, Cooney AL, et al piggyBac transposase tools for genome engineering. Proc Natl Acad Sci. 2013 Jun;18(25):E2279–2287.
   Web of Science ®Google Scholar
• Hirano H, Tanaka K, Ozaki K, et al ROM7/BEM4 encodes a novel protein that interacts with the Rho1p small GTP-binding protein in Saccharomyces cerevisiae . Mol Cell Biol. 1996 Aug;16(8):4396–4403.
   PubMed Web of Science ®Google Scholar
• Mack D, Nishimura K, Dennehey BK, et al. Identification of the bud emergence gene BEM4 and its interactions with rho-type GTPases in Saccharomyces cerevisiae. Mol Cell Biol.. 1996;16:4387–4395.
   PubMed Web of Science ®Google Scholar
• Bendezú FO, Martin SG. Cdc42 explores the cell periphery for mate selection in fission yeast. Curr Biol.. 2013 Jan;23(1):42–47.
   PubMed Web of Science ®Google Scholar
• Melendez J, Grogg M, Zheng Y. Signaling role of Cdc42 in regulating mammalian physiology. J Biol Chem. 2011 Jan;286(286):2375–2381.
   PubMed Web of Science ®Google Scholar
• Horecka J, Jigami Y. Identifying tagged transposon insertion sites in yeast by direct genomic sequencing. Yeast. 2000 Jul;16:967–970.
   PubMed Web of Science ®Google Scholar
• Ross-Macdonald P, Sheehan A, Friddle C, et al Transposon mutagenesis for the analysis of protein production, function, and localization . Methods Enzymol. 1999;303:512–532.
   PubMed Web of Science ®Google Scholar
• Ding S, Wu X, Li Get al Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice . Cell. Aug 2005;122(3):473–483.
   PubMed Web of Science ®Google Scholar
• Handler AM, Harrell RA 2nd. Germline transformation of Drosophila melanogaster with the piggyBac transposon vector. Insect Mol Biol. 1999 Nov;8:449–457.
   PubMed Web of Science ®Google Scholar
• Rad R, Rad L, Wang W, et al PiggyBac transposon mutagenesis: a tool for cancer gene discovery in mice. Science. 2010 Nov;330(6007):1104–1107.
   PubMed Web of Science ®Google Scholar
• Li J, Zhang J-M, Li X, et al. A piggyBac transposon-based mutagenesis system for the fission yeast Schizosaccharomyces pombe. Nucleic Acids Res. 2011;39:e40.
   PubMed Web of Science ®Google Scholar
• Mitra R, Li X, Kapusta A, et al Functional characterization of piggyBat from the bat Myotis lucifugus unveils an active mammalian DNA transposon. Proc Natl Acad Sci. 2013 Jan;110(1):234–239.
   PubMed Web of Science ®Google Scholar
• Drees BL, Sundin B, Brazeau E, et al. A protein interaction map for cell polarity development. J Cell Biol. 2001 Aug;154(3):549–576.
   PubMed Web of Science ®Google Scholar
• Pitoniak A, Chavel CA, Chow J, et al Cdc42p-interacting protein Bem4p regulates the filamentous-growth mitogen-activated protein kinase pathway. Mol Cell Biol. 2015 Jan;35(2):417–436.
   PubMed Web of Science ®Google Scholar