In vitro-derived platelets: the challenges we will have to face to assess quality and safety

References

• Williamson DR, Albert M, Heels-Ansdell D, Arnold DM, Lauzier F, Zarychanski R, Crowther M, Warkentin TE, Dodek P, Cade J, et al. Thrombocytopenia in critically ill patients receiving thromboprophylaxis: frequency, risk factors, and outcomes. Chest. 2013;144(4):1207–1215. doi:10.1378/chest.13-0121.
   PubMed Web of Science ®Google Scholar
• Estcourt LJ, Birchall J, Lowe D, Grant-Casey J, Rowley M, Murphy MF, et al. Platelet transfusions in haematology patients: are we using them appropriately? Vox Sang. 2012;103(4):284–293. doi:10.1111/j.1423-0410.2012.01627.x.
   PubMed Web of Science ®Google Scholar
• Cowan K. Strategies to reduce inappropriate use of platelet transfusions. Nurs Times. 2017;113: 2, 18-21.
   Google Scholar
• Blood NHS & Transplant. NHSBT Price List 2016-17. (2016). Available at: (Accessed: 4th February 2020) https://web.archive.org/web/20180603111712/http://hospital.blood.co.uk/media/28230/component-price-list-2016-2017.pdf
   Google Scholar
• American red cross. one-two punch of winter storms, canceled blood drives straining red cross blood supply. Available at: (Accessed 4 February 2020). https://www.redcrossblood.org/local-homepage/news/article/one-two-punch-of-winter-storms–canceled-blood-drives-straining-.html
   Google Scholar
• Smethurst PA. Aging of platelets stored for transfusion. Platelets. 2016;27(6):526–534. doi10.3109/09537104.2016.1171303.
   PubMed Web of Science ®Google Scholar
• García-Roa M, Del Carmen Vicente-Ayuso M, Bobes AM, Pedraza AC, González-Fernández A, Martín MP, Sáez I, Seghatchian J, Gutiérrez L, et al. Red blood cell storage time & transfusion: current practice, concerns & future perspectives. Blood Transfusion. 2017;15(3):222–231. doi:10.2450/2017.0345-16.
   PubMed Web of Science ®Google Scholar
• Nakamura S, Takayama N, Hirata S, Seo H, Endo H, Ochi K, Fujita K-I, Koike T, Harimoto K-I, Dohda T, et al. Expandable megakaryocyte cell lines enable clinically applicable generation of platelets from human induced pluripotent stem cells. Cell Stem Cell. 2014;14(4):535–548. doi:10.1016/j.stem.2014.01.011.
   PubMed Web of Science ®Google Scholar
• Moreau T,  Evans AL, Vasquez L, Tijssen MR, Yan Y, Trotter MW, Howard D, Colzani M, Arumugam M, Wu WH, Dalby A, et al. Large-scale production of megakaryocytes from human pluripotent stem cells by chemically defined forward programming. Nat Commun. 2016;7:11208doi:10.1038/ncomms11208.
   PubMed Web of Science ®Google Scholar
• Thon JN, Mazutis L, Wu S, Sylman JL, Ehrlicher A, Machlus KR, Feng Q, Lu S, Lanza R, Neeves KB, et al. Platelet bioreactor-on-a-chip. Blood. 2014;124(12):1857–1867. doi:10.1182/blood-2014-05-574913.
   PubMed Web of Science ®Google Scholar
• Ito Y, Nakamura S, Sugimoto N, Shigemori T, Kato Y, Ohno M, Sakuma S, Ito K, Kumon H, Hirose H, et al. Turbulence activates platelet biogenesis to enable clinical scale ex vivo production. Cell. 2018;174(3):636–648.e18. doi:10.1016/j.cell.2018.06.011.
   PubMed Web of Science ®Google Scholar
• Tozzi L, Laurent P-A, Di Buduo CA, Mu X, Massaro A, Bretherton R, Stoppel W, Kaplan DL, Balduini A, et al. Multi-channel silk sponge mimicking bone marrow vascular niche for platelet production. Biomaterials. 2018;178:122–133doi:10.1016/j.biomaterials.2018.06.018.
   PubMed Web of Science ®Google Scholar
• Merkle FT, Ghosh S, Kamitaki N, Mitchell J, Avior Y, Mello C, Kashin S, Mekhoubad S, Ilic D, Charlton M, et al. Human pluripotent stem cells recurrently acquire and expand dominant negative P53 mutations. Nature. 2017;545(7653):229–233. doi:10.1038/nature22312.
   PubMed Web of Science ®Google Scholar
• Baker D, Hirst A, Gokhale P, Juarez M, Williams S, Wheeler M, Bean K, Allison T, Moore H, Andrews P, et al. Detecting genetic mosaicism in cultures of human pluripotent stem cells. Stem Cell Rep. 2016;7(5):998–1012. doi:10.1016/j.stemcr.2016.10.003.
   PubMed Web of Science ®Google Scholar
• Zhang J, Hirst AJ, Duan F, Qiu H, Huang R, Ji Y, Bai L, Zhang F, Robinson D, Jones M, et al. Anti-apoptotic mutations desensitize human pluripotent stem cells to mitotic stress and enable aneuploid cell survival. Stem Cell Rep. 2019;12(3):557–571. doi:10.1016/j.stemcr.2019.01.013.
   PubMed Web of Science ®Google Scholar
• Na J, Baker D, Zhang J, Andrews PW, Barbaric I. Aneuploidy in pluripotent stem cells and implications for cancerous transformation. Protein Cell. 2014;5(8):569–579. doi10.1007/s13238-014-0073-9.
   PubMed Web of Science ®Google Scholar
• Merve A, et al. c-MYC overexpression induces choroid plexus papillomas through a T-cell mediated inflammatory mechanism. Acta Neuropathol Commun. 2019;7:2.
   PubMed Web of Science ®Google Scholar
• Soucek L, Evan GI. The ups and downs of Myc biology. Curr Opin Genet Dev. 2010;20(1):91–95. doi10.1016/j.gde.2009.11.001.
   PubMed Web of Science ®Google Scholar
• Allison TF, Andrews PW, Avior Y, Barbaric I, Yamanaka S. Assessment of established techniques to determine developmental and malignant potential of human pluripotent stem cells. Nat Commun. 2018;9:1925
   PubMed Web of Science ®Google Scholar
• Blin A, Le Goff A, Magniez A, Poirault-Chassac S, Teste B, Sicot G, Nguyen KA, Hamdi FS, Reyssat M, Baruch D, et al. Microfluidic model of the platelet-generating organ: beyond bone marrow biomimetics. Sci. Rep. 2016;6(1). doi:10.1038/srep21700
   PubMedGoogle Scholar
• Pallotta I, Lovett M, Kaplan DL, Balduini A. Three-dimensional system for the in vitro study of megakaryocytes and functional platelet production using silk-based vascular tubes. Tissue Eng. 2011;17(12):1223–1232. doi10.1089/ten.tec.2011.0134.
   Google Scholar
• Shepherd JH, Howard D, Waller AK, Foster HR, Mueller A, Moreau T, Evans AL, Arumugam M, Bouët Chalon G, Vriend E, et al. Structurally graduated collagen scaffolds applied to the ex vivo generation of platelets from human pluripotent stem cell-derived megakaryocytes: enhancing production and purity. Biomaterials. 2018;182:135–144doi:10.1016/j.biomaterials.2018.08.019.
   PubMed Web of Science ®Google Scholar
• Stacey GN, Andrews PW, Barbaric I, Boiers C, Chandra A, Cossu G, Csontos L, Frith TJ, Halliwell JA, Hewitt Z, et al. Stem cell culture conditions and stability: A joint workshop of the plurimes consortium and pluripotent stem cell platform. Regen. Med. 2019;14(3):243–255. doi:10.2217/rme-2019-0001.
   PubMed Web of Science ®Google Scholar
• Cardigan R, Turner C, Harrison P. Current methods of assessing platelet function: relevance to transfusion medicine. Vox Sang. 2005;88(3):153–163. doi10.1111/j.1423-0410.2005.00618.x.
   PubMed Web of Science ®Google Scholar
• Born GV. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature. 1962;194(4832):927–929. doi10.1038/194927b0.
   PubMed Web of Science ®Google Scholar
• Finkenstaedt-Quinn SA, Ge S, Haynes CL. Cytoskeleton dynamics in drug-treated platelets. Anal. Bioanal. Chem. 2015;407(10):2803–2809. doi10.1007/s00216-015-8523-7.
   PubMed Web of Science ®Google Scholar
• Roest M, Reininger A, Zwaginga JJ, King MR, Heemskerk JWM. Flow chamber-based assays to measure thrombus formation in vitro: requirements for standardization. J Thrombosis Haemostasis. 2011;9(11):2322–2324. doi10.1111/j.1538-7836.2011.04492.x.
   PubMed Web of Science ®Google Scholar
• Feghhi S, Munday AD, Tooley WW, Rajsekar S, Fura AM, Kulman JD, López JA, Sniadecki NJ, et al. Glycoprotein Ib-IX-V complex transmits cytoskeletal forces that enhance platelet adhesion. Biophys. J. 2016;111(3):601–608. doi:10.1016/j.bpj.2016.06.023.
   PubMed Web of Science ®Google Scholar
• Newman PJ, Aster R, Boylan B. Human platelets circulating in mice: applications for interrogating platelet function and survival, the efficacy of antiplatelet therapeutics, and the molecular basis of platelet immunological disorders. J Thromb Haemost. 2007;5:305–309. doi10.1111/j.1538-7836.2007.02466.x.
   PubMed Web of Science ®Google Scholar
• Hu Z, Yang YG. Full reconstitution of human platelets in humanized mice after macrophage depletion. Blood. 2012;120(8):1713–1716. doi10.1182/blood-2012-01-407890.
   PubMed Web of Science ®Google Scholar
• Rosen ED, Raymond S, Zollman A, Noria F, Sandoval-Cooper M, Shulman A, Merz JL, Castellino FJ, et al. Laser-induced noninvasive vascular injury models in mice generate platelet- and coagulationdependent thrombi. Am. J. Pathol. 2001;158(5):1613–1622. doi:10.1016/S0002-9440(10)64117-X.
   PubMed Web of Science ®Google Scholar
• Suzuki D, Flahou C, Yoshikawa N, Stirblyte I, Hayashi Y, Sawaguchi A, Akasaka M, Nakamura S, Higashi N, Xu H, et al. iPSC-derived platelets depleted of HLA class I are inert to anti-HLA class I and natural killer cell immunity. Stem Cell Rep. 2020;14(1):49–59. doi:10.1016/j.stemcr.2019.11.011.
   PubMed Web of Science ®Google Scholar