Immunoblotting of gliadins separated by acid PAGE: Analysis of electrotransference conditions

References

• Bietz , J. A. and Simpson , D. G. 1992 . Electrophoresis and chromatography of wheat proteins: available methods, and procedures for statistical evaluation of the data . Journal of Chromatography , 624 : 53 – 80 .
   PubMed Web of Science ®Google Scholar
• Brett , G. M. , Mills , E. N. , Parmar , S. , Tatham , A. S. , Shewry , P. R. and Morgan , M. R. 1990 . Monoclonal antibodies that recognize the repeat motif of the S‐poor prolamins . Journal of Cereal Science , 12 : 245 – 255 .
   Web of Science ®Google Scholar
• Bushuk , W. and Zillman , R. R. 1978 . Wheat cultivar identification by gliadin electrophoregrams . Canadian Journal of Plant Science , 58 : 505 – 515 .
   Web of Science ®Google Scholar
• Chirdo , F. G. , Fossati , C. A. and A[nbar]ón , M. C. 1995 . Optimisation of a competitive ELISA with polyclonal antibodies for quantification of prolamins in foods . Food and Agricultural Immunology , 7 : 333 – 343 .
   Web of Science ®Google Scholar
• Denery‐Papini , S. , Briand , J. P. , Quellien , L. , Popineau , Y. and Regenmore , M. H. V. 1994 . Immunological differentiation of various gliadins and low M1 subunits of glutenin using anti‐peptide antisera . Journal of Cereal Science , 20 : 1 – 14 .
   Web of Science ®Google Scholar
• Festenstein , G. N. , Hay , F. C. and Shewry , P. R. 1987 . Immunochemical relationships of the prolamin storage proteins of barley, wheat, rye and oats . Biochimica Biophysica Acta , 912 : 371 – 383 .
   Web of Science ®Google Scholar
• Freedman , A. R. , Wieser , H. , Ellis , H. J. and Ciclitira , P. J. 1988 . Immunoblotting of gliadins separated by reversed‐phase high‐performance liquid chromatography: detection with monoclonal antibodies . Journal of Cereal Science , 8 : 22 – 29 .
   Web of Science ®Google Scholar
• Friis , S. U. and Schäfer‐Nielsen , C. 1985 . Separation of gliadin at pH 3.1 in a polyacrylamide gel suitable for blotting procedures . Journal of Biochemical and Biophysical Methods , 10 : 301 – 306 .
   PubMedGoogle Scholar
• Harlow , E. and Lane , D. 1988 . “ Immunoblotting protocols ” . In Antibodies, a Laboratory Manual , 494 – 495 . New York : Cold Spring Harbor .
   Google Scholar
• Janssen , F. W. , Baaij , J. A. and Hagele , G. H. 1994 . Heat treated meat products. Detection of modified gluten by SDS‐electrophoresis, western‐blotting and immunochemical staining . Fleischwirtsch , 74 : 168 – 179 .
   Google Scholar
• Lafiandra , D. and Kasarda , D. D. 1985 . One‐ and two‐dimensional polyacrylamide gel electrophoresis in a single gel: separation of wheat proteins . Cereal Chemistry , 62 : 314 – 319 .
   Web of Science ®Google Scholar
• Mccarthy , P. K. , Lumley , I. D. and Griffin , M. 1992 . “ Discrimination between hexaploid and durum wheat using anti‐gliadin antisera ” . In Food Safety and Quality Assurance: Applications of Immunoassay Systems Edited by: Morgan , M. R. A. , Smith , C. J. and Williams , P. A. 411 – 416 .
   Google Scholar
• Mills , C. E. N. , Burgess , S. R. , Tatham , A. S. , Shewry , P. R. , Chan , H. W. S. and Morgan , M. R. A. 1990 . Characterization of a panel of monoclonal anti‐gliadin antibodies . Journal of Cereal Science , 11 : 89 – 101 .
   Web of Science ®Google Scholar
• Mills , E. N. C. , Brett , G. M. , Holden , S. , Kauffman , J. A. , Tatton , M. J. and Morgan , M. R. A. 1995 . Production of monoclonal antibodies to gluten proteins and their use in developing tests for gluten quality . Food and Agricultural Immunology , 7 : 189 – 196 .
   Web of Science ®Google Scholar
• Skerritt , J. H. and Hill , A. S. 1990 . Monoclonal antibody sandwich enzyme immunoassays for determination of gluten in foods . Journal of Agricultural and Food Chemistry , 38 : 1771 – 1778 .
   Web of Science ®Google Scholar
• Skerritt , J. H. and Lew , P. Y. 1990 . Homologies between grain storage proteins of different cereal species. 1. Monoclonal antibody reaction with total protein extracts . Journal of Cereal Science , 11 : 103 – 121 .
   Web of Science ®Google Scholar
• Skerritt , J. H. and Smith , R. A. 1985 . A sensitive monoclonal‐antibody based test for gluten detection: studies with cooked or processed foods . Journal of the Science of Food and Agriculture , 36 : 980 – 986 .
   Web of Science ®Google Scholar