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International Journal of Radiation Biology Volume 99, 2023 - Issue 1: 9th International Symposium on Physical, Molecular, Cellular and Medical Aspects of Auger Processes
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Original Articles

Comet Assay analysis of DNA strand breaks after exposure to the DNA-incorporated Auger Electron Emitter Iodine-125

Marcus Unverricht-Yeboaha Department of Safety and Radiation Protection, Forschungszentrum Jülich, Jülich, GermanyView further author information
,
Kathrin Holtmannb Brenk Systemplanung GmbH, Aachen, GermanyView further author information
&
Ralf Kriehubera Department of Safety and Radiation Protection, Forschungszentrum Jülich, Jülich, GermanyCorrespondence[email protected]
View further author information
Pages 64-69 | Received 22 Apr 2020, Accepted 03 Nov 2020, Published online: 03 Dec 2020

References

  • Balagurumoorthy P, Chen K, Adelstein SJ, Kassis AI. 2008. Auger electron-induced double-strand breaks depend on DNA topology. Radiat Res. 170:70–82.
  • Boyd M, Sorensen A, McCluskey AG, Mairs RJ. 2008. Radiation quality-dependent bystander effects elicited by targeted radionuclides. J Pharm Pharmacol. 60:951–958.
  • Choucroun P, Gillet D, Dorange G, Sawicki B, Dewitte J. 2001. Comet assay and early apoptosis. Mutat Res. 478:89–96.
  • Christensen RC, Tobias C, Taylor W. 1972. Heavy-ion-induced single- and double-strand breaks in phiX-174 replicative form DNA. Int J Radiat Biol Relat Stud Phys Chem Med. 22:457–477.
  • Godard T, Deslandes E, Lebailly P, Vigreux C, Poulain L, Sichel F, Poul JM, Gauduchon P. 1999. Comet assay and DNA flow cytometry analysis of staurosporine‐induced apoptosis. Cytometry. 36:117–122.
  • Gopalakrishna P, Khar A. 1995. Comet assay to measure DNA damage in apoptotic cells. J Biochem Biophys Methods. 30:69–73.
  • Kassis AI, Adelstein SJ. 2005. Radiobiologic principles in radionuclide therapy. J Nucl Med. 46:4S–12S.
  • Kassis A, Sastry K, Adelstein S. 1987. Kinetics of uptake, retention, and radiotoxicity of 125IUdR in mammalian cells: implications of localized energy deposition by Auger processes. Radiat Res. 109:78–89.
  • Kizilian N, Wilkins R, Reinhardt P, Ferrarotto C, McLean J, McNamee J. 1999. Silver-stained comet assay for detection of apoptosis. Biotechniques. 27:926–930.
  • Kohn KW. 1991. Principles and practice of DNA filter elution. Pharmacol Ther. 49:55–77.
  • Kriehuber R, Riedling M, Simkó M, Weiss DG. 2004. Cytotoxicity, genotoxicity and intracellular distribution of the Auger electron emitter (65)Zn in two human cell lines. Radiat Environ Biophys. 43:15–22.
  • Luke AM, Chastain PD, Pachkowski BF, Afonin V, Takeda S, Kaufman DG, Swenberg JA, Nakamura J. 2010. Accumulation of true single strand breaks and AP sites in base excision repair deficient cells. Mutat Res. 694:65–71.
  • Olive PL, Durand RE. 2005. Heterogeneity in DNA damage using the comet assay. Cytometry Part A. 66:1–8.
  • Olive PL, Frazer G, Banáth JP, Banath JP. 1993. Radiation-induced apoptosis measured in TK6 human B lymphoblast cells using the comet assay. Radiat Res. 136:130–136.
  • Ostling O, Johanson KJ. 1984. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem Biophys Res Commun. 123:291–298.
  • Pomplun E, Terrissol M, Demonchy M. 1996. Modelling of initial events and chemical behaviour of species induced in DNA units by Auger electrons from 125I, 123I and carbon. Acta Oncol. 35:857–862.
  • Pomplun E, Terrissol M, Hille R. 2002. Ratio of complex double strand break damage induced by 125IUdR and 123IUdR correlates with experimental in vitro cell killing effectiveness. Radiat Prot Dosimetry. 99:81–82.
  • Rogakou EP, Pilch DR, Orr AH, Ivanova VS, Bonner WM. 1998. DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J Biol Chem. 273:5858–5868.
  • Singh NP, McCoy MT, Tice RR, Schneider EL. 1988. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 175:184–191.
  • Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF. 2000. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen. 35:206–221.
  • Tilgen W, Boukamp P, Breitkreutz D, Dzarlieva RT, Engstner M, Haag D, Fusenig NE. 1983. Preservation of morphological, functional, and karyotypic traits during long-term culture and in vivo passage of two human skin squamous cell carcinomas. Cancer Res. 43:5995–6011.
  • Unverricht-Yeboah M, Giesen U, Kriehuber R. 2018. Comparative gene expression analysis after exposure to 123I-iododeoxyuridine, gamma- and alpha-radiation-potential biomarkers for the discrimination of radiation qualities. J Radiat Res. 59:411–429.
  • Ward JF. 1988. DNA damage produced by ionizing radiation in mammalian cells: identities, mechanisms of formation, and reparability. Prog Nucleic Acid Res Mol Biol. 35;95–125.
  • Wilkins RC, Kutzner B, Truong M, Sanchez‐Dardon J, McLean J. 2002. Analysis of radiation‐induced apoptosis in human lymphocytes: Flow cytometry using Annexin V and propidium iodide versus the neutral comet assay. Cytometry. 48:14–19.

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