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Original Articles

A simplified protocol for gene expression-based biological dosimetry using peripheral whole blood

ORCID Icon & ORCID Icon
Pages 1692-1701 | Received 06 Jan 2023, Accepted 08 Jun 2023, Published online: 12 Jul 2023

References

  • Abend M, Badie C, Quintens R, Kriehuber R, Manning G, Macaeva E, Njima M, Oskamp D, Strunz S, Moertl S, et al. 2016. Examining radiation-induced in vivo and in vitro gene expression changes of the peripheral blood in different laboratories for biodosimetry purposes: first RENEB gene expression study. Radiat Res. 185(2):109–123.
  • Abend M, Amundson SA, Badie C, Brzoska K, Hargitai R, Kriehuber R, Schüle S, Kis E, Ghandhi SA, Lumniczky K, et al. 2021. Inter-laboratory comparison of gene expression biodosimetry for protracted radiation exposures as part of the RENEB and EURADOS WG10 2019 exercise. Sci Rep. 11(1):9756.
  • Agbenyegah S, Abend M, Atkinson MJ, Combs SE, Trott KR, Port M, Majewski M. 2018. Impact of inter-individual variance in the expression of a radiation-responsive gene panel used for triage. Radiat Res. 190(3):226–235.
  • Amundson SA, Do KT, Shahab S, Bittner M, Meltzer P, Trent J, Fornace J. 2000. Identification of potential mRNA biomarkers in peripheral blood lymphocytes for human exposure to ionizing radiation. Radiat Res. 154(3):342–346.2.0.CO;2]
  • Andrysik Z, Kim J, Tan AC, Espinosa JM. 2013. A genetic screen identifies TCF3/E2A and TRIAP1 as pathway-specific regulators of the cellular response to p53 activation. Cell Rep. 3(5):1346–1354.
  • Badie C, Kabacik S, Balagurunathan Y, Bernard N, Brengues M, Faggioni G, Greither R, Lista F, Peinnequin A, Poyot T, et al. 2013. Laboratory intercomparison of gene expression assays. Radiat Res. 180(2):138–148.
  • Baird BJ, Dickey JS, Nakamura AJ, Redon CE, Parekh P, Griko YV, Aziz K, Georgakilas AG, Bonner WM, Martin OA. 2011. Hypothermia postpones DNA damage repair in irradiated cells and protects against cell killing. Mutat Res. 711(1-2):142–149.
  • Belloni P, Meschini R, Palitti F. 2008. Effects of storage conditions of human whole bloodon the viability of lymphocytes. Int J Radiat Biol. 84(7):613–619.
  • Bhagi S, Alok A, Chaudhury N. 2021. Radiation induced gene expression signatures for triage biodosimetry. Def Life Sc Jl. 6(1):85–93.
  • Bieback K, Ha VA, Hecker A, Grassl M, Kinzebach S, Solz H, Sticht C, Kluter H, Bugert P. 2010. Altered gene expression in human adipose stem cells cultured with fetal bovine serum compared to human supplements. Tissue Eng Part A. 16(11):3467–3484.
  • Broustas CG, Harken AD, Garty G, Amundson SA. 2018. Identification of differentially expressed genes and pathways in mice exposed to mixed field neutron/photon radiation. BMC Genomics. 19(1):504.
  • Broustas CG, Duval AJ, Amundson SA. 2021. Impact of aging on gene expression response to x-ray irradiation using mouse blood. Sci Rep. 11(1):10177.
  • Brzóska K, Kruszewski M. 2015. Toward the development of transcriptional biodosimetry for the identification of irradiated individuals and assessment of absorbed radiation dose. Radiat Environ Biophys. 54(3):353–363.
  • Budworth H, Snijders AM, Marchetti F, Mannion B, Bhatnagar S, Kwoh E, Tan Y, Wang SX, Blakely WF, Coleman M, et al. 2012. DNA repair and cell cycle biomarkers of radiation exposure and inflammation stress in human blood. PLoS One. 7(11):e48619.
  • Chauhan V, Howland M. 2014. Gene expression responses in human lung fibroblasts exposed to alpha particle radiation. Toxicol in Vitro. 28(7):1222–1229.
  • Field LA, Jordan RM, Hadix JA, Dunn MA, Shriver CD, Ellsworth RE, Ellsworth DL. 2007. Functional identity of genes detectable in expression profiling assays following globin mRNA reduction of peripheral blood samples. Clin Biochem. 40(7):499–502.
  • Fukunaga H. 2020. The effect of low temperatures on environmental radiation damage in living systems: does hypothermia show promise for space travel? IJMS. 21(17):6349.
  • Garty G, Bigelow AW, Repin M, Turner HC, Bian D, Balajee AS, Lyulko OV, Taveras M, Yao YL, Brenner DJ. 2015. An automated imaging system for radiation biodosimetry. Microsc Res Tech. 78(7):587–598.
  • Ghandhi SA, Smilenov LB, Elliston CD, Chowdhury M, Amundson SA. 2015. Radiation dose-rate effects on gene expression for human biodosimetry functional and structural. BMC Med Genomics. 8:22.
  • Ghandhi SA, Sima C, Weber WM, Melo DR, Rudqvist N, Morton SR, Turner HC, Amundson SA. 2020. Dose and dose-rate effects in a mouse model of internal exposure to 137Cs. Part 1: global Transcriptomic Responses in Blood. Radiat Res. 196:478–490.
  • Grievink HW, Moerland M. 2018. Sample aging profoundly reduces monocyte responses in human whole blood cultures. J Immunol Res. 2018:8901485.
  • Gruel G, Grégoire E, Lecas S, Martin C, Roch-Lefevre S, Vaurijoux A, Voisin P, Voisin P, Barquinero JF. 2013. Biological dosimetry by automated dicentric scoring in a simulated emergency. Radiat Res. 179(5):557–569.
  • International Atomic Energy Agency. 2011. Cytogenetic dosimetry: applications in preparedness for and response to radiation emergencies. Vienna, Austria: IAEA; p. 1–279.
  • Jen K, Cheung VG. 2003. Transcriptional response of lymphoblastoid cells to ionizing radiation. Genome Res. 13(9):2092–2100.
  • Kabacik S, Mackay A, Tamber N, Manning G, Finnon P, Paillier F, Ashworth A, Bouffler S, Badie C. 2011. Gene expression following ionising radiation: identification of biomarkers for dose estimation and prediction of individual response. Int J Radiat Biol. 87(2):115–129.
  • Kannan K, Amariglio N, Rechavi G, Jakob-Hirsch J, Kela I, Kaminski N, Getz G, Domany E, Givol D. 2001. DNA microarrays identification of primary and secondary target genes regulated by p53. Oncogene. 20(18):2225–2234.
  • Knops K, Boldt S, Wolkenhauer O, Kriehuber R. 2012. Gene expression in low- and high-dose-irradiated human peripheral blood lymphocytes: possible applications for biodosimetry. Radiat Res. 178(4):304–312.
  • Kulka U, Abend M, Ainsbury E, Badie C, Barquinero JF, Barrios L, Beinke C, Bortolin E, Cucu A, De Amicis A, et al. 2017. RENEB - Running the European Network of biological dosimetry and physical retrospective dosimetry. Int J Radiat Biol. 93(1):2–14.
  • Lacombe J, Sima C, Amundson SA, Zenhausern F. 2018. Candidate gene biodosimetry markers of exposure to external ionizing radiation in human blood: A systematic review. PLoS One. 13(6):e0198851.
  • Li S, Lu X, Feng JB, Tian M, Wang J, Chen H, Chen DQ, Liu QJ. 2019. Developing gender-specific gene expression biodosimetry using a panel of radiation-responsive genes for determining radiation dose in human peripheral blood. Radiat Res. 192(4):399–409.
  • Li W, Zhou S, Jia M, Li X, Li L, Wang Q, Qi Z, Zhou P, Li Y, Wang Z. 2022. Early biomarkers associated with P53 signaling for acute radiation injury. Life (Basel). 12(1):99.
  • Lisowska H, Cheng L, Sollazzo A, Lundholm L, Wegierek-Ciuk A, Sommer S, Lankoff A, Wojcik A. 2018. Hypothermia modulates the DNA damage response to ionizing radiation in human peripheral blood lymphocytes. Int J Radiat Biol. 94(6):551–557.
  • Liu J, Walter E, Stenger D, Thach D. 2006. Effects of globin mRNA reduction methods on gene expression profiles from whole blood. J Mol Diagn. 8(5):551–558.
  • Macaeva E, Mysara M, De Vos WH, Baatout S, Quintens R. 2019. Gene expression-based biodosimetry for radiological incidents: assessment of dose and time after radiation exposure. Int J Radiat Biol. 95(1):64–75.
  • Manning G, Kabacik S, Finnon P, Bouffler S, Badie C. 2013. High and low dose responses of transcriptional biomarkers in ex vivo X-irradiated human blood. Int J Radiat Biol. 89(7):512–522.
  • Manning G, Macaeva E, Majewski M, Krie Huber R, Brzóska K, Abend M, Doucha-Senf S, Oskamp D, Strunz S, Quintens R, et al. 2017. Comparable dose estimates of blinded whole blood samples are obtained independently of culture conditions and analytical approaches. Second RENEB gene expression study. Int J Radiat Biol. 93(1):87–98.
  • Meadows SK, Dressman HK, Daher P, Himburg H, Russell JL, Doan P, Chao NJ, Lucas J, Nevins JR, Chute JP. 2010. Diagnosis of partial body radiation exposure in mice using peripheral blood gene expression profiles. PLoS One. 5(7):e11535.
  • Nahm MH, Chatham WW, Benjamin WH. Jr. 2012. Device for carrying blood samples at 37 °C for cryoglobulin test. Clin Vaccine Immunol. 19(9):1555–1556.
  • Nasser F, Cruz-Garcia L, O'Brien G, Badie C. 2021. Role of blood derived cell fractions, temperature and sample transport on gene expression-based biological dosimetry. Int J Radiat Biol. 97(5):675–686.
  • O'Brien G, Cruz-Garcia L, Majewski M, Grepl J, Abend M, Port M, Tichý A, Sirak I, Malkova A, Donovan E, et al. 2018. FDXR is a biomarker of radiation exposure in vivo. Sci Rep. 8(1):684.
  • Oestreicher U, Samaga D, Ainsbury E, Antunes AC, Baeyens A, Barrios L, Beinke C, Beukes P, Blakely WF, Cucu A, et al. 2017. RENEB intercomparisons applying the conventional dicentric chromosome assay (DCA). Int J Radiat Biol. 93(1):20–29.
  • Paul S, Amundson SA. 2008. Development of gene expression signatures for practical radiation biodosimetry. Int J Radiat Oncol Biol Phys. 71(4):1236–1244.
  • Port M, Herodin F, Valente M, Drouet M, Lamkowski A, Majewski M, Abend M. 2016. First generation gene expression signature for early prediction of late occurring hematological acute radiation syndrome in baboons. Radiat Res. 186(1):39–54.
  • Schwochow D, Serieys LE, Wayne RK, Thalmann O. 2012. Efficient recovery of whole blood RNA-a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species. BMC Biotechnol. 12:33.
  • Zou N, Xie G, Cui T, Srivastava AK, Qu M, Yang L, Wei S, Zheng Y, Wang QE. 2016. DDB2 increases radioresistance of NSCLC cells by enhancing DNA damage responses. Tumour Biol. 37(10):14183–14191.

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