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Original Articles

Extending culture time to improve Mitotic Index for cytogenetic dosimetry

ORCID Icon, ORCID Icon, ORCID Icon, ORCID Icon, ORCID Icon & ORCID Icon
Pages 1029-1040 | Received 17 Nov 2022, Accepted 13 May 2024, Published online: 24 May 2024

References

  • Amaral A, De Lima S, Silva L, Lemos-Pinto M, Lucena L, Marques-Salles T, Silva E, Magnata S. 2019. A new methodology for diagnosis of Fanconi anemia based on biological dosimetry. Arch Biosci Heal. 1(2):189–200.
  • Averbeck D, Candéias S, Chandna S, Foray N, Friedl AA, Haghdoost S, Jeggo PA, Lumniczky K, Paris F, Quintens R, et al. 2019. Establishing mechanisms affecting the individual response to ionizing radiation. Int J Radiat Biol. 96(3):297–323. doi:10.1080/09553002.2019.1704908
  • Balakrishnan S, Shirsath k, Bhat N, Anjaria K. 2010. Biodosimetry for high dose accidental exposures by drug induced premature chromosome condensation (PCC) assay. Mutat Res. 699(1–2):11–16. doi:10.1016/j.mrgentox.2010.03.008
  • Beaton LA, Ferrarotto C, Marro L, Samiee S, Malone S, Grimes S, Malone K, Wilkins RC. 2013. Chromosome damage and cell proliferation rates in in vitro irradiated whole blood as markers of late radiation toxicity after radiation therapy to the prostate. Int J Radiat Oncol. 85(5):1346–1352. doi:10.1016/j.ijrobp.2012.09.026
  • Bender MA, Gooch PC. 1962. Persistent chromosome aberrations in irradiated human subjects. Radiat Res. 16(1):44. https://www.jstor.org/stable/3571128?origin=crossref.
  • Bender MA, Prescott DM. 1962. DNA synthesis and mitosis in cultures of human peripheral leukocytes. Exp Cell Res. 27(2):221–229.
  • van den Berg J, Manjón AG, Kielbassa K, Feringa FM, Freire R, Medema RH. 2018. A limited number of double-strand DNA breaks is sufficient to delay cell cycle progression. Nucleic Acids Res. 46(19):10132–10144. doi:10.1093/nar/gky786
  • Bhattacharyya B, Panda D, Gupta S, Banerjee M. 2008. Anti-mitotic activity of colchicine and the structural basis for its interaction with tubulin. Med Res Rev. 28(1):155–183.
  • Borgmann K, Hoeller U, Nowack S, Bernhard M, Röper B, Brackrock S, Petersen C, Szymczak S, Ziegler A, Feyer P, et al. 2008. Individual radiosensitivity measured with lymphocytes may predict the risk of acute reaction after radiotherapy. Int J Radiat Oncol Biol Phys. 71(1):256–264.
  • Casella G. 2008. Statistical design. New York, NY: Springer New York. 10.1007/978-0-387-75965-4.
  • Chao HX, Poovey CE, Privette AA, Grant GD, Chao HY, Cook JG, Purvis JE. 2017. Orchestration of dna damage checkpoint dynamics across the human cell cycle. Cell Syst. 5(5):445–459.e5. https://linkinghub.elsevier.com/retrieve/pii/S2405471217304362. doi:10.1016/j.cels.2017.09.015
  • Chen D q, Zhang C y 1992. A simple and convenient method for gaining pure populations of lymphocytes at the first mitotic division in vitro. Mutat Res Lett. 282(3):227–229.
  • Chen Y, Yan XK, Du J, Wang ZD, Zhang XQ, Zeng FG, Zhou PK. 2011. Biological dose estimation for accidental supra-high dose gamma-ray exposure. Radiat Meas. 46(9):837–841.
  • Chua MLK, Somaiah N, A'Hern R, Davies S, Gothard L, Yarnold J, Rothkamm K. 2011. Residual DNA and chromosomal damage in ex vivo irradiated blood lymphocytes correlated with late normal tissue response to breast radiotherapy. Radiother Oncol. 99(3):362–366. doi:10.1016/j.radonc.2011.05.071
  • Deperas J, Szłuíska M, Deperas-Kaminska M, Edwards A, Lloyd D, Lindholm C, Romm H, Roy L, Moss R, Morand J, et al. 2007. CABAS: A freely available PC program for fitting calibration curves in chromosome aberration dosimetry. Radiat Prot Dosimetry. 124(2):115–123.
  • Derrick B, Ruck A, Toher D, White P. 2018. Tests for equality of variances between two samples which contain both paired observations and independent observations. J Appl Quant Methods. 13(2):36–47.
  • Edwards A, Voisin P, Sorokine-Durm I, Maznik N, Vinnikov V, Mikhalevich L, Moquet J, Lloyd D, Delbos M, Durand V. 2004. Biological estimates of dose to inhabitants of Belarus and Ukraine following the Chernobyl accident. Radiat Prot Dosimetry. 111(2):211–219.
  • Fernandes TS, Loyd DC, Amaral A. 2008. Biodosimetry for dose assessment of partial-body exposure: a methodological improvement. Brazilian Arch Biol Technol. 51(SPECIALISSUE):97–102.
  • Florian S, Mitchison TJ. 2016. Anti-microtubule drugs. Methods Mol Biol. 1413:403–421. 10.1007/978-1-4939-3542-0_25.
  • Giussani A, Lopez MA, Romm H, Testa A, Ainsbury EA, Degteva M, Della Monaca S, Etherington G, Fattibene P, Güclu I, et al. 2020. Eurados review of retrospective dosimetry techniques for internal exposures to ionising radiation and their applications. Radiat Environ Biophys. 59(3):357–387. doi:10.1007/s00411-020-00845-y
  • Hall EJ, Giaccia AJ. 2018. Radiobiology for the radiologist. 8th ed. Philadelphia: Wolters Kluwer Health.
  • Heimers A, Brede HJ, Giesen U, Hoffmann W. 2006. Chromosome aberration analysis and the influence of mitotic delay after simulated partial-body exposure with high doses of sparsely and densely ionising radiation. Radiat Environ Biophys. 45(1):45–54. doi:10.1007/s00411-006-0036-5
  • Henegariu O, Heerema NA, Wright LL, Bray-Ward P, Ward DC, Vance GH. 2001. Improvements in cytogenetic slide preparation: Controlled chromosome spreading, chemical aging and gradual denaturing. Cytometry. 43(2):101–109. doi:10.1002/1097-0320(20010201)43:2<101::AID-CYTO1024>3.0.CO;2-8
  • Herate C, Sabatier L. 2020. Retrospective biodosimetry techniques: Focus on cytogenetics assays for individuals exposed to ionizing radiation. Mutat Res Rev Mutat Res. 783:108287. doi:10.1016/j.mrrev.2019.108287
  • International Atomic Energy Agency. 2005. Generic procedures for medical response during a nuclear or radiological emergency. Vienna: IAEA.
  • International Atomic Energy Agency. 2006. Manual for first responders to a radiological emergency. Vienna: IAEA.
  • International Atomic Energy Agency. 2011. Cytogenetic dosimetry : applications in preparedness for and response to radiation emergencies. Vienna: IAEA.
  • International Atomic Energy Agency. 2020. Guidance for medical physicists responding to a nuclear or radiological emergency, emergency preparedness and response. Vienna: IAEA.
  • Kaddour A, Colicchio B, Buron D, El Maalouf E, Laplagne E, Borie C, Ricoul M, Lenain A, Hempel WM, Morat L, et al. 2017. Transmission of induced chromosomal aberrations through successive mitotic divisions in human lymphocytes after in vitro and vivo radiation. Sci Rep. 7(1):3291. doi:10.1038/s41598-017-03198-7
  • Kanda R, Hayata I, Lloyd DC. 1999. Easy biodosimetry for high-dose radiation exposures using drug-induced, prematurely condensed chromosomes. Int J Radiat Biol. 75(4):441–446.
  • Kanda R, Jiang T, Hayata I, Kobayashi S. 1994. Effects of colcemid concentration on chromosome aberration analysis in human lymphocytes. J Radiat Res. 35(1):41–47.
  • Karachristou I, Karakosta M, Pantelias A, Hatzi V, Pantelias G, Thanassoulas A, Karaiskos P, Dimitriou P, Terzoudi GI. 2016. Biodosimetry for high-dose exposures based on dicentric analysis in lymphocytes released from the g2-block by caffeine. Radiat Prot Dosimetry. 172(1–3):230–237. doi:10.1093/rpd/ncw151
  • Khvostunov IK, Snigiryova GP, Moiseenko VV, Lloyd DC. 2015. A follow-up cytogenetic studyofworkers highly exposed inside the chernobyl sarcophagus. Radiat Prot Dosimetry. 167(4):405–418. doi:10.1093/rpd/ncu351
  • Krishnaja AP, Sharma NK. 2004. Transmission of γ-ray-induced unstable chromosomal aberrations through successive mitotic divisions in human lymphocytes in vitro. Mutagenesis. 19(4):299–305. doi:10.1093/mutage/geh031
  • Kulka U, Wojcik A, Di Giorgio M, Wilkins R, Suto Y, Jang S, Quing-Jie L, Jiaxiang L, Ainsbury E, Woda C, et al. 2018. Biodosimetry and biodosimetry networks for managing radiation emergency. Radiat Prot Dosimetry. 182(1):128–138. doi:10.1093/rpd/ncy137
  • Lamadrid AI, García O, Delbos M, Voisin P, Roy L. 2007. PCC-ring induction in human lymphocytes exposed to gamma and neutron irradiation. J Radiat Res. 48(1):1–6.
  • Lemos-Pinto MMP, Cadena M, Santos N, Fernandes TS, Borges E, Amaral A. 2015. A dose-response curve for biodosimetry from a 6 MV electron linear accelerator. Brazilian J Med Biol Res. 48(10):908–914.
  • De Lemos Pinto MMP, Santos NFG, Amaral A. 2010. Current status of biodosimetry based on standard cytogenetic methods. Radiat Environ Biophys. 49(4):567–581. doi:10.1007/s00411-010-0311-3
  • Maznik NA, Vinnikov VA, Lloyd DC, Edwards AA. 1997. Chromosomal dosimetry for some groups of evacuees from Prypiat and Ukranian liquidators at Chernobyl. Radiat Prot Dosimetry. 74(1–2):5–11.
  • Moiseenko V, Khvostunov IK, Hattangadi-Gluth JA, Muren LP, Lloyd DC. 2016. Biological dosimetry to assess risks of health effects in victims of radiation accidents: thirty years after Chernobyl. Radiother Oncol. 119(1):1–4. doi:10.1016/j.radonc.2016.02.033
  • Moquet J, Rothkamm K, Barnard S, Ainsbury E. 2020. Radiation biomarkers in large scale human health effects studies. J Pers Med. 10(4):1–14.
  • Pernot E, Hall J, Baatout S, Benotmane MA, Blanchardon E, Bouffler S, El Saghire H, Gomolka M, Guertler A, Harms-Ringdahl M, et al. 2012. Ionizing radiation biomarkers for potential use in epidemiological studies. Mutat Res. 751(2):258–286. doi:10.1016/j.mrrev.2012.05.003
  • Perry P, Wolff S. 1974. New Giemsa method for the differential staining of sister chromatids. Nature. 251(5471):156–158. http://www.nature.com/articles/251156a0. doi:10.1038/251156a0
  • Pujol M, Puig R, Caballín MR, Barrios L, Barquinero JF. 2012. The use of caffeine to assess high dose exposures to ionising radiation by dicentric analysis. Radiat Prot Dosimetry. 149(4):392–398.
  • Purrott RJ, Vulpis N, Lloyd DC. 1980. The use of harlequin staining to measure delay in the human lymphocyte cell cycle induced by in vitro X-irradiation. Mutat Res Mol Mech Mutagen. 69(2):275–282.
  • Purrott RJ, Vulpis N, Lloyd DC. 1981a. The influence of incubation temperature on the rate of human lymphocyte proliferation in vitro. Experientia. 37(4):407–408. doi:10.1007/BF01959890
  • Purrott RJ, Vulpis N, Lloyd DC. 1981b. Chromosome dosimetry: the influence of culture media on the proliferation of irradiated and unirradiated human lymphocytes. Radiat Prot Dosimetry. 1(3):203–208.
  • Razali NM, Wah YB. 2011. Power comparisons of Shapiro–Wilk, Kolmogorov–Smirnov, Lilliefors and Anderson–Darling tests. J Stat Model Anal. 2(1):21–33.
  • Roy L, Gregoire E, Gruel G, Roch-Lefevre S, Voisin P, Busset A, Martin C, Voisin P. 2012. Effect of lymphocytes culture variations on the Mitotic Index and on the dicentric yield following gamma radiation exposure. Radiat Prot Dosimetry. 151(1):135–143.
  • Sakamoto-Hojo ET. 2018. Lessons from the accident with 137Cesium in Goiania, Brazil: Contributions to biological dosimetry in case of human exposure to ionizing radiation. Mutat Res Genet Toxicol Environ Mutagen. 836(Pt A):72–77. doi:10.1016/j.mrgentox.2018.05.019
  • Santivasi WL, Xia F. 2014. Ionizing radiation-induced DNA damage, response, and repair. Antioxidants Redox Signal. 21(2):251–259.
  • Scully R, Panday A, Elango R, Willis NA. 2019. DNA double-strand break repair-pathway choice in somatic mammalian cells. Nat Rev Mol Cell Biol. 20(11):698–714. doi:10.1038/s41580-019-0152-0
  • Seeber A, Hauer MH, Gasser SM. 2018. Chromosome dynamics in response to DNA damage. Annu Rev Genet. 52(1):295–319. doi:10.1146/annurev-genet-120417-031334
  • Sevan’kaev AV, Lloyd DC, Braselmann H, Edwards AA, Moiseenko VV, Zhloba AA. 1995. A Survey of chromosomal aberrations in lymphocytes of Chernobyl liquidators. Radiat Prot Dosimetry. 58(2):85–91. doi:10.1093/oxfordjournals.rpd.a082602
  • Sevan’kaev AV, Lloyd DC, Edwards AA, Khvostunov IK, Mikhailova GF, Golub EV, Shepel NN, Nadejina NM, Galstian IA, Nugis VY, et al. 2005. A cytogenetic follow-up of some highly irradiated victims of the Chernobyl accident. Radiat Prot Dosimetry. 113(2):152–161. doi:10.1093/rpd/nch435
  • Sevan’kaev AV, Lloyd DC, Edwards AA, Moiseenko VV. 1995. High exposures to radiation received by workers inside the Chernobyl sarcophagus. Radiat Prot Dosimetry. 59(2):85–91. doi:10.1093/oxfordjournals.rpd.a082641
  • Shaltiel IA, Krenning L, Bruinsma W, Medema RH. 2015. The same, only different – DNA damage checkpoints and their reversal throughout the cell cycle. J Cell Sci. 128(4):607–620.
  • Sun M, Moquet J, Barnard S, Lloyd D, Ainsbury E. 2020. A simplified Calyculin A-induced premature chromosome condensation (PCC) protocol for the biodosimetric analysis of high-dose exposure to gamma radiation. Radiat Res. 193(6):560–568.
  • Vinnikov V, Belyakov O. 2022. Clinical applications of biological dosimetry in patients exposed to low dose radiation due to radiological, imaging or nuclear medicine procedures. Semin Nucl Med. 52(2):114–139. doi:10.1053/j.semnuclmed.2021.11.008
  • Vinnikov V, Hande MP, Wilkins R, Wojcik A, Zubizarreta E, Belyakov O. 2020. Prediction of the acute or late radiation toxicity effects in radiotherapy patients using ex vivo induced biodosimetric markers: a review. J Pers Med. 10(4):285. doi:10.3390/jpm10040285
  • Vinnikov VA, Ainsbury EA, Maznyk NA, Lloyd DC, Rothkamm K. 2010. Limitations associated with analysis of cytogenetic data for biological dosimetry. Radiat Res. 174(4):403–414.
  • Vinnikov VA, Belyakov O. 2020. Radiation exposure biomarkers in the practice of medical radiology: Cooperative Research and the Role of the International Atomic Energy Agency (IAEA) Biodosimetry/Radiobiology Laboratory. Health Phys. 119(1):83–94. doi:10.1097/HP.0000000000001266
  • Vinnikov VA, Maznyk NA. 2013. Cytogenetic dose-response in vitro for biological dosimetry after exposure to high doses of gamma-rays. Radiat Prot Dosimetry. 154(2):186–197.
  • Waterman DP, Haber JE, Smolka MB. 2020. Checkpoint responses to DNA double-strand breaks. Annu Rev Biochem. 89(1):103–133. doi:10.1146/annurev-biochem-011520-104722
  • Wenzel ES, Singh ATK. 2018. Cell-cycle checkpoints and aneuploidy on the path to cancer. In Vivo. 32(1):1–5.
  • Yao B, Jiang BR, Ai HS, Li YF, Liu GX, Man QH, Qiu LJ. 2010. Biological dose estimation for two severely exposed patients in a radiation accident in Shandong Jining, China, in 2004. Int J Radiat Biol. 86(9):800–808.

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