Flow Cytometric Analysis of the Effects of 0·4 MeV Fission Neutrons on Mouse Spermatogenesis

References

• Bateman J.L., Johnson H.A., Bond N.P., Rossi H.H. The dependence of RBE on the energy of fast neutrons for spermatogonia depletion in mice. Radiation Research 1968; 35: 86–101
   PubMed Web of Science ®Google Scholar
• Bianchi M. Cytotoxic insult to germinal tissue: Part I, Testis. Cytotoxic Insult to Tissue, C.S. Potten, J.H. Hendry. Churchill Livingstone, Edinburgh 1983; 259–307
   Google Scholar
• Bianchi M., Delic J.I., Hurtado De Catalfo G., Hendry J.H. Strain differences in the radiosensitivity of mouse spermatogonia. International Journal of Radiation Biology 1985; 48: 579–588
   Web of Science ®Google Scholar
• Calvin H.I., Bedford S.M. Formation of disulphide bonds in the nucleus and accessory structures of mammalian spermatozoa during maturation in the epididymis. Journal of Reproduction and Fertility (Suppl.) 1971; 13: 65–75
   Google Scholar
• Clausen O.P.F., Abyholm F. Deoxyribonucleic acid flow cytometry of germ cells in the investigation of male infertility. Fertility and Sterility 1980; 34: 369–373
   PubMed Web of Science ®Google Scholar
• Clausen O.P.F., Parvinen M., Kirkhus B. Stage-related variations in DNA fluorescence distributions during rat spermatogonic cycle as revealed by flow cytometry. Cytometry 1982; 2: 421–425
   Google Scholar
• Covelli V., Di Majo V., Bassani B., Rebessi S., Coppola M., Silini G. Influence of age on life shortening and tumor induction after X-ray and neutron irradiation. Radiation Research 1984; 100: 348–364
   PubMed Web of Science ®Google Scholar
• De Ruiter-Bootsma A.L., Kramer M.F., De Rooij D.G. Response of stem cells in the mouse testis to fission neutrons of 1 MeV mean energy and 300 kV X-rays. Methodology, dose-response studies, relative biological effectiveness. Radiation Research 1976; 67: 56–68
   PubMed Web of Science ®Google Scholar
• De Ruiter-Bootsma A.L., Kramer M.F., De Rooij D.G. Survival of spermatogonial stem cells in the mouse after split-dose irradiation with fission neutrons of 1 MeV mean energy. Effects of the fractionation interval. Radiation Research 1979; 79: 289–297
   Google Scholar
• Di Paola M., Caffarelli V., Coppola M., Porro F., Quintiliani M., Baarli J., Bianchi M., Sullivan A.H. Biological responses to various neutron energies from 1 to 600 MeV. Radiation Research 1980; 84: 444–452
   PubMed Web of Science ®Google Scholar
• Dym M., Clermont Y. Effects of X-rays on type A spermatogonia in the rat. Anatomical Records 1967; 157: 238–243
   Google Scholar
• Eisert W.G., Mendelsohn M.L. Biological Dosimetry. Springer-Verlag, Berlin, Heidelberg 1984
   Google Scholar
• Evenson D.P., Melamed M.R. Rapid analysis of normal and abnormal cell types in human semen and testis biopsies by flow cytometry. Journal of Histochemistry and Cytochemistry 1983; 31: 248–253
   Web of Science ®Google Scholar
• Evenson D.P., Higgins P.J., Gruenenberg D., Ballachey B.E. Flow cytometric analysis of mouse spermatogenic function following exposure to ethylnitrosourea. Cytometry 1985; 6: 238–253
   PubMedGoogle Scholar
• Geraci J.P., Jackson K.L., Christensen G.M., Thrower P.D., Weyer B.J. Mouse testes as a biological test system for intercomparison of fast neutron beams. Radiation Research 1977; 71: 377–386
   PubMed Web of Science ®Google Scholar
• Göhde W., Schumann J., Büchner T., Otto F., Barlogie B. Pulse cytophotometry. Application in tumor cell biology and clinical oncology. Flow Cytometry and Sorting, M.R. Melamed, P.F. Mullaney, M.L. Mendelsohn. John Wiley, New York 1979; 559–620
   Google Scholar
• Grosch D.S., Hopwood L.E. Biological Effects of Radiations. Academic Press, New York 1979; 211–224
   Google Scholar
• Hacker U., Schumann J., Göhde W. Effects of acute gamma-irradiation on spermatogenesis as revealed by flow cytometry. Acta Radiologica Oncology 1980; 19: 361–368
   PubMed Web of Science ®Google Scholar
• Hacker U., Schumann J., Göhde W., Müller K. Mammalian spermatogenesis as a biologic dosimeter for radiation. Acta Radiologica Oncology 1981; 20: 279–282
   PubMed Web of Science ®Google Scholar
• Hacker U., Schumann J., Göhde W. Mammalian spermatogenesis as a new system for biologic dosimetry of ionizing radiation. Acta Radiologica Oncology 1982; 21: 349–351
   PubMed Web of Science ®Google Scholar
• Hacker-Klom U., Göhde W., Schumann J. Mammalian spermatogenesis as a biological dosimeter for ionizing radiation. Biological Dosimetry, W.G. Eisert, M.L. Mendelsohn. Springer-Verlag, Berlin, Heidelberg 1984; 127–137
   Google Scholar
• Hagaki G. The effect of X-rays of 1000R on male germ cells in the mouse, with particular attention to the difference in X-ray sensitivity of spermatogonia. Japanese Journal of Genetics 1970; 45: 239–254
   Google Scholar
• Hansmann I., Janke D., Probeck H.D. Cytogenetic studies in mammalian oogenesis and spermatogenesis on the induction of nondisjunction and structural chromosome anomalies, with special consideration of the genetic risk in the F1 population. Euratom Radiation Protection Progress Report 1980; 659–664
   Google Scholar
• ICRU. Radiobiological Dosimetry, International Commission on Radiation Units and Measurements. Washington DC 1963, Report 10e
   Google Scholar
• Kohn H.I., Kallman R.F. Testis weight loss as a quantitative measure of X-ray injury in the mouse, hamster and rat. British Journal of Radiology 1956; 27: 586–591
   Google Scholar
• Kramer H.F., Davids J.A.G., Van der Ven T.P.A. Effects of 1 MeV fast-neutron irradiation on spermatogonial proliferation in mice: influence of dose fractionation with different intervals. International Journal of Radiation Biology 1974; 25: 253–260
   Google Scholar
• Lu C.C., Meistrich M.L., Thames H.D. Survival of mouse testicular stem cells after gamma or neutron irradiation. Radiation Research 1980; 81: 402–415
   PubMed Web of Science ®Google Scholar
• Meistrich M.L., Reid B.O., Barcellona W.J. Changes in sperm nuclei during spermatogenesis and epididymal maturation. Experimental Cell Research 1976; 99: 72–78
   PubMed Web of Science ®Google Scholar
• Meistrich M.L., Trostle P.K., Frapart M., Erickson R.P. Biosynthesis and localisation of lactate dehydrogenase X to pachytene spermatocytes and spermatids of mouse testes. Developmental Biology 1977; 60: 428–441
   PubMedGoogle Scholar
• Meistrich M.L., Göhde W., White R.A., Schumann J. Resolution of X and Y spermatids by pulse cytophotometry. Nature 1978 a; 274: 821–823
   PubMedGoogle Scholar
• Meistrich M.L., Hunter N.R., Suzuki N., Trostle P.K., Withers H.R. Gradual regeneration of mouse testicular stem cells after exposure to ionizing radiation. Radiation Research 1978 b; 74: 349–362
   PubMed Web of Science ®Google Scholar
• Meistrich M.L., Lake S., Steinmetz L.L., Gledhill B.L. Flow cytometry of DNA mouse sperm and testis nuclei. Mutation Research 1978 c; 49: 383–396
   Google Scholar
• Meistrich M.L., Lake S., Steinmetz L.L., Gledhill B.L. Increased variability in nuclear DNA content of testis cells and spermatozoa from mice with irregular meiotic segregation. Mutation Research 1978 d; 49: 387–405
   Google Scholar
• Meistrich M.L., Finch M., Lu C.C., De Ruiter-Bootsma A.L., De Rooij D.G., Davids J.A.D. Strain differences in the response of mouse testicular stem cells to fractionated radiation. Radiation Research 1984; 97: 478–487
   PubMed Web of Science ®Google Scholar
• Monesi V. Autoradiographic study of DNA synthesis and the cell cycle in spermatogonia and spermatocytes of mouse testis using tritiated thymidine. Journal of Cell Biology 1962; 14: 1–18
   PubMed Web of Science ®Google Scholar
• Monesi V. Autoradiographic evidence of a nuclear histone synthesis during mouse spermatogenesis in the absence of detectable quantities of nuclear ribonucleaic acid. Experimental Cell Research 1964; 36: 683–688
   PubMedGoogle Scholar
• Muramatsu S., Nakamura W., Eto H. Relative biological effectiveness of X-rays and fast neutrons in inducing translocations in mouse spermatogonia. Mutation Research 1973; 19: 343–347
   Google Scholar
• Oakberg E.F. A description of spermiogenesis in the mouse and its use in analysis of the cycle of the seminiferous epithelium and germ cell renewal. American Journal of Anatomy 1956 a; 99: 391–414
   PubMedGoogle Scholar
• Oakberg E.F. Duration of spermatogenesis in the mouse and timing of stages of the cycle of the seminiferous epithelium. American Journal of Anatomy 1956 b; 99: 507–516
   PubMedGoogle Scholar
• Oakberg E.F. Gamma-ray sensitivity of spermatogonia of the mouse. Journal of Experimental Zoology 1957; 134: 343–355
   PubMedGoogle Scholar
• Oakberg E.F. A new concept of spermatogonial stem-cell renewal in the mouse and its relationship to genetic effect. Mutation Research 1971 a; 11: 1–7
   PubMedGoogle Scholar
• Oakberg E.F. Spermatogonial stem-cell renewal in the mouse. Anatomical Record 1971 b; 169: 515–531
   PubMedGoogle Scholar
• Oakberg E.F. Effect of radiation on the testis. Male Reproductive System, D.W. Hamilton, R.O. Greep. William & Wilkins, Baltimore 1975; 233–243
   Google Scholar
• Oakberg E.F., Huckins C. Spermatogonial stem cell renewal in the mouse as revealed by 3H-thymidine labeling and irradiation. Stem Cells of Renewing Cell Populations, A.B. Cairnie, P.K. Cala, D.G. Osmond. Academic Press, New York 1976; 287–302
   Google Scholar
• Otto F., Hacker U., Zante J., Schumann J., Göhde W., Meistrich M.L. Flow cytometry of human spermatozoa. Histochemistry 1979; 61: 248–254
   Google Scholar
• Otto F.J., Oldiges H. Flow cytogenetic studies in chromosome and whole cells for the detection of clastogenic effects. Cytometry 1980; 1: 13–17
   PubMedGoogle Scholar
• Otto F.J., Oldiges H., Göhde W., Jain V.R. Flow cytometric measurement of nuclear DNA content variations as a potential in vivo mutagenicity test. Cytometry 1981; 2: 189–191
   PubMedGoogle Scholar
• Otto F., Oldiges H., Jain V.K. Flow cytometric measurement of cellular DNA content dispersion induced by mutagenic treatment. Biological Dosimetry, W.G. Eisert, M.L. Mendelsohn. Springer-Verlag, Berlin, Heidelberg 1984; 37–49
   Google Scholar
• Pacchierotti, F., Russo, A., Metalli, P. Meiotic non-disjunction induced by fission neutrons relative to X-rays observed in mouse secondary spermatocytes. II. Dose effect relationships after treatment of pachytene cells. Mutation Research, in the press, 1987
   Google Scholar
• Pfitzer P., Gilbert Q., Rölz G., Vyska K. Flow cytometry of human testicular tissue. Cytometry 1982; 3: 116–122
   Google Scholar
• Pinkel D. Cytometric analysis of mammalian sperm for induced morphologic and DNA content errors. Biological Dosimetry, W.G. Eisert, M.L. Mendelsohn. Springer-Verlag, Berlin, Heidelberg 1984; 111–126
   Google Scholar
• Pinkel D., Gledhill B.L., Van Dilla M.A., Lake S., Stephenson D., Wyrobek A.J. High resolution DNA content measurements in mammalian sperm. Cytometry 1982; 3: 1–9
   PubMedGoogle Scholar
• Pinkel D., Gledhill B.L., Van Dilla M.A., Lake S., Wyrobek A.J. Radiation-induced variability in mouse sperm. Radiation Research 1983; 95: 550–565
   Google Scholar
• Russo A., Pacchierotti F., Metalli P. Meiotic non-disjunction induced by fission neutrons relative to X-rays observed in mouse secondary spermatocytes. I. The response of different cell stages to a single radiation dose. Mutation Research 1983; 108: 359–372
   Google Scholar
• Scarpa G., Aceto M., Moscati M., Furetta C. Further studies on the mixed field of neutrons and gammas in a fast reactor using solid state dosimeters. Proceedings of the Third Symposium on Neutron Dosimetry in Biology and Medicine, G. Burger, H.G. Ebert. Commission of the European Communities, Luxembourg 1978; 737–746
   Google Scholar
• Scarpa G., Moscati M. A dosimetric study of a facility for biological experiments in a fast reactor. Monograph on Basic Physical Data for Neutron Dosimetry, J.J. Broerse. Commission of the European Communities Report EUR 5629, EEC, Luxembourg 1976; 17–22
   Google Scholar
• Searle A.G., Evans E.P., West B.J. Studies on the induction of translocations in mouse spermatogonia, II. Effects of post neutron irradiation. Mutation Research 1969; 7: 235–240
   Google Scholar
• Sotomajor R.E., Sega G.A., Cummings R.E. An autoradiographic study of unscheduled DNA synthesis in the germ cells of male mice treated with X-rays and methyl methansulfonate. Mutation Research 1979; 62: 293–309
   Google Scholar
• Stolla R., Gropp A. Variation of the DNA content of morphologically normal and abnormal spermatozoa in mice susceptible to irregular meiotic segregation. Journal of Reproduction and Fertility 1974; 38: 335–346
   PubMedGoogle Scholar
• Tates A.D., de Vogel N. Further studies on effects of X-irradiation on prespermatid stages of the northern vole Microtus oeconomus. Low induction of sex-chromosomal nondisjunction and very high induction of diploid spermatids. Mutation Research 1981; 82: 323–330
   Google Scholar
• Tates A.D., Pearson P.L., Van der Ploeg M., de Vogel N. The induction of sex-chromosomal non disjunction and diploid spermatids following X-irradiation of pre-spermatid stages in the northern vole Microtus oeconomus. Mutation Research 1979; 61: 87–101
   PubMed Web of Science ®Google Scholar
• Van Kroonenburg M.J., Beck J.L., Scholtz J.W., Hacker-Klom U., Herman C.J. DNA analysis and sorting of rat testis cells using two-parameter flow cytometry. Cytometry 1985; 6: 321–326
   Google Scholar
• Withers H.R., Hunter N., Barkley H.T., Jr., Reid B.O. Radiation survival and regeneration characteristics of spermatogenic stem cells in mouse testis. Radiation Research 1974; 57: 88–103
   PubMed Web of Science ®Google Scholar
• Wyrobeck A.J. Changes in mammalian sperm morphology after X-ray and chemical exposure. Genetics 1979; 92: 105–119
   Google Scholar
• Wyrobeck A.J., Gordon L.A., Burkhart J.G., Francis M.W., Kapp R.W., Jr., Letz G., Malling H.V., Topham J.C., Whorton M.D. An evaluation of the mouse sperm morphology test and other sperm tests in non human mammals: A report of the U.S. Environmental Protection Agency Gene Tox Program. Mutation Research 1983; 115: 1–72
   Google Scholar
• Zante J., Schumann J., Göhde W., Hacker U. DNA-fluorometry of mammalian sperm. Histochemistry 1977; 54: 1–7
   PubMedGoogle Scholar