14
Views
5
CrossRef citations to date
0
Altmetric
Original Article

Technical Communication

A Single-shot Rapid-mixing Device for Radiobiological Studies with Mammalian Cells

, , &
Pages 705-715 | Received 17 Jun 1988, Accepted 15 Dec 1988, Published online: 03 Jul 2009

References

  • Adams G.E., Cooke M.S., Michael B.D. Rapid-mixing in radiobiology. Nature 1968; 219: 1368–1369
  • Alexander P., Charlesby A. Physico-chemical methods of protection against ionising radiations. Radiobiology Symposium, Z.M. Bacq, P. Alexander. Butterworths, London 1955; 49–60
  • Boye E., Johansen I., Brustad T. Time scale for rejoining of bacteriophage λ deoxyribonucleic acid molecules in superinfected Pol+ and Pol A1 strains of E. coli after exposure to 4 MeV electrons. Journal of Bacteriology 1974; 119: 522–533
  • Denekamp J., Rojas A., Stevens G. Redox competition and radiosensitivity: implications for testing radioprotective compounds. Pharmacology and Therapeutics 1988, in press
  • Dewey D.L., Michael B.D. The mechanism of radiosensitization by iodoacetamide. Biophysical and Biochemical Research Communications 1965; 21: 392–396
  • Eccleston J.F., Messerschmidt R.G., Yates D.W. A simple rapid-mixing device. Analytical Biochemistry 1980; 106: 73–77
  • Fox R.A., Fielden E.M., Sapora O. Yield of single-strand breaks in the DNA of E. coli 10 msec after irradiation. International Journal of Radiation Biology 1976; 29: 391–394
  • Hodgkiss R.J., Middleton R.W. Enhancement of misonidazole radiosensitization by an inhibitor of glutathione biosynthesis. International Journal of Radiation Biology 1983; 43: 179–183
  • Hodgkiss R.J., Stratford M.R.L. Competitive dose-modification between ascorbate and misonidazole in human and hamster cells: effects of glutathione depletion. International Journal of Radiation Biology 1988; 54: 601–610
  • Hodgkiss R.J., Jones N.R., Watts M.E., Woodcock M. Glutathione depletion enhances the lifetime of oxygen-reactive radicals in mammalian cells. International Journal of Radiation Biology 1984; 46: 673–674
  • Hodgkiss R.J., Roberts I.J., Watts M.E., Woodcock M. Rapid-mixing studies with thiol-depleted mammalian cells. International Journal of Radiation Biology 1987; 52: 735–744
  • Howard-Flanders P., Alper T. The sensitivity of microorganisms to irradiation under controlled gas conditions. Radiation Research 1957; 7: 518–540
  • Howard-Flanders P., Moore D. The time interval after pulsed irradiation within which injury to bacteria can be modified by dissolved oxygen. Radiation Research 1958; 9: 422–437
  • Kandaiya S. Radioprotection of radiation damage fixed by misonidazole in Chinese hamster cells a rapid-mix study. Radiation Research 1986; 105: 272–275
  • Koch C.J., Howell R.L. Combined radiation-protective and radiation sensitizing agents. II. Radiosensitivity of hypoxic or aerobic Chinese hamster fibroblasts in the presence of cysteamine and misonidazole: implications for the ‘oxygen effect’ (with appendix on calculation of dose-modifying factors). Radiation Research 1981; 87: 265–283
  • Ling C.C., Michaels H.B., Epp E.R., Peterson E.C. Oxygen diffusion rates into mammalian cells following ultrahigh dose rate irradiation and lifetime estimates of oxygen sensitive species. Radiation Research 1978; 76: 522–532
  • Michael B.D., Adams G.E., Hewitt H.B., Jones W.B.G., Watts M.E. A posteffect of oxygen in irradiated bacteria: a submillisecond fast mixing study. Radiation Research 1973; 54: 239–251
  • Michael B.D., Harrop H.A., Maughan R.L., Patel K.B. A fast kinetics study of the modes of action of some different radiosensitizers in bacteria. British Journal of Cancer 1978; 37(Suppl. III)29–33
  • Michael B.D., Harrop H.A. Timescale and mechanism of radiosensitization and radioprotection at the cellular level. Radiation Sensitizers: Their use in the clinical management of cancer, L.W. Brady. Masson, USA 1980; 14–21
  • Michael B.D., Davies S., Held K. Ultrafast chemical repair of DNA single and double rand break precursors in irradiated V79 cells. Mechanisms of DNA damage and repair, M.G. Simic, L. Grossman, A.C. Upton. Plenum Press, New York 1986; 89–100
  • Penner S. Calculation of properties of magnetic deflection systems. Reviews of Scientific Instruments 1961; 32: 150–159
  • Sapora O., Fielden E.M., Loverock P.S. The application of rapid-lysis techniques in radiobiology. I. The effect of oxygen and radiosensitizers on DNA strand-break production and repair in E. coli B/r. Radiation Research 1975; 64: 431–442
  • Shenoy M.A., Asquith J.C., Adams G.E., Michael B.D., Watts M.E. Time-resolved oxygen effects in irradiated bacteria and mammalian cells: a rapid-mix study. Radiation Research 1975; 62: 498–512
  • Stratford I.J., Maughan R.L., Michael B.D., Tallentire A. The decay of potentially lethal oxygen-dependent damage in fully hydrated Bacillus megaterium spores exposed to pulsed electron irradiation. International Journal of Radiation Biology 1977; 32: 447–455
  • Vojnovic B. Sensitive long pulse beam charge monitor for use with charged particle accelerators. Radiation Physics Chemistry 1987; 29: 409–413
  • Watts M.E., Maughan R.L., Michael B.D. Fast kinetics of the oxygen effect in irradiated mammalian cells. International Journal of Radiation Biology 1978; 33: 195–199
  • Watts M.E., Hodgkiss R.J., Jones N.R., Sehmi D.S., Woodcock M. A rapid-mix study on the effect of lipophilicity of nitroimidazoles on the radiosensitization of mammalian cells in vitro. International Journal of Radiation Biology 1983; 43: 329–336
  • Whillans D.W. A rapid-mixing system for radiobiological studies using mammalian cells. Radiation Research 1982; 90: 109–125
  • Whillans D.W., Hunt J.W. Rapid-mixing studies of the mechanism of chemical radiosensitization and protection in mammalian cells. British Journal of Cancer 1978; 37(Suppl. III)38–41
  • Whillans D.W., Hunt J.W. A rapid-mixing comparison of the mechanisms of radiosensitization by oxgen and misonidazole in CHO cells. Radiation Research 1982; 90: 126–141
  • Willson R.L. Free radical repair mechanisms and the interactions of glutathione and vitamins C and E. Radioprotectors and Anticarcinogens, O.V. Nygaard, M.G. Simic. Academic Press, London 1983; 1–22

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.