26
Views
34
CrossRef citations to date
0
Altmetric
Original Article

Analysis by Pulsed-field Gel Electrophoresis of DNA Double-strand Breakage and Repair in Deinococcus Radiodurans and a Radiosensitive Mutant

, , &
Pages 613-626 | Received 03 Jul 1990, Accepted 18 Mar 1991, Published online: 03 Jul 2009

References

  • Ager D.D., Dewey W.C., Gardiner K., Harvey W., Johnson R.T., Waldren C.A. Measurement of radiation-induced DNA double-strand breaks by pulsed-field gel electrophoresis. Radiation Research 1990; 122: 181–187
  • Blöcher D., Einspenner M., Zajackowski J. CHEF electrophoresis, a sensitive technique for the determination of DNA double-strand breaks. International Journal of Radiation Biology 1989; 56: 437–448
  • Brooks B.W., Murray R.G.E. Nomenclature for ‘Micrococcus radiodurans’ and other radiation-resistant cocci: Deinococcaceae fam. nov. and Deinococcus gen. nov., including five species. International Journal of Systematic Bacteriology 1981; 31: 353–360
  • Contopoulou C.R., Cook V.E., Mortimer R.K. Analysis of DNA double strand breakage and repair using orthogonal field alternation gel electrophoresis. Yeast 1987; 3: 71–76
  • Dean C.J., Feldschreiber P., Lett J.T. Repair of x-ray damage to the deoxyribonucleic acid in Micrococcus radiodurans. Nature 1966; 209: 49–52
  • Driedger A.A., Grayston M.J. Rapid lysis of cell walls of Miccrococcus radiodurans with lysozyme: effects of butanol pre-treatment on DNA. Canadian Journal of Microbiology 1970; 16: 889–893
  • Evans D.M. Repair of DNA damage in Deinococcus radiodurans. University of Edinburgh. 1985, Ph.D. thesis
  • Gardiner K., Laas W., Patterson D. Fractionation of large mammalian DNA restriction fragments using vertical pulsed-field gradient gel electrophoresis. Somatic Cell and Molecular Genetics 1986; 12: 185–195
  • Gillis M., De Ley J., De Cleene M. The determination of molecular weight of bacterial genome DNA from renaturation rates. European Journal of Biochemistry 1970; 12: 143–153
  • Hansen M.T. Multiplicity of genome equivalents in the radiation-resistant bacterium Micrococcus radiodurans. Journal of Bacteriology 1978; 134: 71–75
  • Kitayama S., Matsuyama A. Possibility of the repair of double strand scissions in Micrococcus radiodurans DNA caused by gamma-rays. Biochemical and Biophysical Research Communications 1968; 33: 418–422
  • Kitayama S., Matsuyama A. Double-strand scissions in DNA of gamma-irradiated Micrococcus radiodurans and their repair during postirradiation incubation. Agricultural and Biological Chemistry 1971; 35: 644–652
  • Lai E., Birren B.W., Clark S.M., Simon M.I., Hood L. Pulsed field gel electrophoresis. Biotechniques 1989; 7: 34–42
  • Lennon E., Minton K.W. Gene fusions with lacZ by duplication insertion in the radioresistant bacterium Deinococcus radiodurans. Journal of Bacteriology 1990; 172: 2955–2961
  • Moseley B.E.B. Photobiology and radiobiology of Micrococcus (Deinococcus) radiodurans. Photochemical and Photobiological Reviews 1983; 7: 223–275
  • Murray R.G.E. The biology and ecology of the Deinococcaceae. Perspectives in Microbial Ecology, F. Megusar, M. Gantar, 1986; 153–158, (Proc. IV. Int. Symp. Microbial Ecology)
  • Roberts R.J. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Research 1984; 12: r167–r204
  • Smith C.L., Cantor C.R. Purification, specific fragmentation and separation of large DNA molecules. Methods in Enzymology 1987; 155: 449–467
  • Smith C.L., Econome J.G., Schutt A., Klco S., Cantor C.R. A physical map of the Escherichia coli K12 genome. Science 1987; 236: 1448–1453
  • Smith M.D., Lennon E., McNeil L.B., Minton K.W. Duplication insertion of drug resistance determinants in the radioresistant bacterium Deinococcus radiodurans. Journal of Bacteriology 1988; 170: 2126–2135
  • Stamato T.D., Denko N. Assymetric field inversion gel electrophoresis: a new method for detecting DNA double-strand breaks in mammalian cells. Radiation Research 1990; 121: 196–205
  • Tirgari S., Moseley B.E.B. Transformation in Micrococcus radiodurans: measurement of various parameters and evidence for multiple, independently segregating genomes per cell. Journal of General Microbiology 1980; 119: 287–297

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.