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International Journal of Radiation Biology Volume 60, 1991 - Issue 5
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Original Article

Comparison between Pulsed-field and Constant-field Gel Electrophoresis for Measurement of DNA Double-strand Breaks in Irradiated Chinese Hamster Ovary Cells

D. Wlodek Medical Biophysics Unit, British Columbia Cancer Research Centre, 601 W. 10th Avenue, Vancouver, B.C., V5Z 1L3, Canada
,
J. Banáth Medical Biophysics Unit, British Columbia Cancer Research Centre, 601 W. 10th Avenue, Vancouver, B.C., V5Z 1L3, Canada
&
P.L. Olive Medical Biophysics Unit, British Columbia Cancer Research Centre, 601 W. 10th Avenue, Vancouver, B.C., V5Z 1L3, Canada
Pages 779-790 | Received 14 Jan 1991, Accepted 29 Apr 1991, Published online: 03 Jul 2009

References

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  • Blöcher D. In CHEF electrophoresis a linear induction of dsb corresponds to a nonlinear fraction of extracted DNA with dose. International Journal of Radiation Biology 1990; 57: 7–12
  • Blöcher D., Einspenner M., Zajackowski J. CHEF electrophoresis, a sensitive technique for the determination of DNA double-strand breaks. International Journal of Radiation Biology 1989; 56: 437–448
  • Blöcher D., Kunhi M. DNA double-strand break analysis by CHEF electrophoresis. International Journal of Radiation Biology 1990; 58: 23–34
  • Chen C.Z., Sutherland J.C. Gel electrophoresis method for quantitation of gamma ray induced single- and double-strand breaks in DNA irradiated in vitro. Electrophoresis 1989; 10: 318–326
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  • Roots R., Kraft G., Gosschalk E. The formation of radiation-induced DNA breaks: the ratio of double-strand breaks to single-strand breaks. International Journal of Radiation Oncology Biology and Physics 1985; 11: 259–265
  • Schwartz D.C., Cantor C.R. Separation of yeast chromosome-sized DNAs by pulsed-field gradient gel electrophoresis. Cell 1984; 37: 67–75
  • Smith C., Matsumoto T., Niwa O., Klco F., Fan J., Yanagida M., Cantor C.R. An electrophoretic karyotype for Saccharomyces pombe by pulsed field electrophoresis. Nucleic Acids Research 1987; 15: 4481–4489
  • Stamato T.D., Denko N. Asymmetric field inversion gel electrophoresis: a new method for detecting DNA double-strand breaks in mammalian cells. Radiation Research 1990; 121: 196–205

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