Search for Oncogene Mutations in X-ray-transformed Mouse 10T½ Cells by Denaturing Gradient Gel Electrophoresis Blotting

References

• Abrams E.S., Murdaugh S.E., Lerman L.S. Comprehensive detection of single base changes in human genomic DNA using denaturing gradient gel electrophoresis and a GG clamp. Genomics 1990; 7: 463–475
   PubMed Web of Science ®Google Scholar
• Abrams E.S., Murdaugh S.E., Lerman L.S. Comprehensive screening of the human K-RAS-2 gene for sequence variants. Genes, Chromosomes and Cancer 1993; 6: 73–85
   PubMedGoogle Scholar
• Baylin S.B., Makos M., Wu J., Chiu Yen R.-W., deBustros A., Vertino P., Nelkin B.D. Abnormal patterns of DNA methylation in human neoplasia: potential consequences for tumor progression. Cancer Cells 1991; 3: 383–390
   PubMed Web of Science ®Google Scholar
• Behrens J., Warram J.H., Lerman L.S., Krolewski A.S. User-friendly PC program to obtain DNA melting map. American Journal of Human Genetics 1990; 47(Supp. I)A173–A173
   Google Scholar
• Bernard O., Cory S., Gerondakis S., Webb E., Adams J.M. Sequence of the murine and human cellular myc oncogenes and two modes of myc transcription resulting from chromosome translocation in B lymphoid tumours. EMBO Journal 1983; 2: 2375–2383
   PubMed Web of Science ®Google Scholar
• Billings P.C., Shuin T., Lillehaug J., Miura T., Roy-Burman P., Landolph J.R. Enhanced expression and state of the c-myc oncogene in chemically and X-ray transformed C3H/10T½ Cl 8 mouse embryo fibroblasts. Cancer Research 1987; 47: 3643–3649
   PubMed Web of Science ®Google Scholar
• Borek C., Ong A., Mason H. Distinctive transforming genes in X-ray-transformed mammalian cells. Proceedings of the National Academy of Sciences, USA 1987; 84: 794–798
   PubMed Web of Science ®Google Scholar
• Brown K., Bailleul B., Ramsden M., Fee F., Krumlauf R., Balmain A. Isolation and characterization of the 5′ flanking region of the mouse c-Harvey-ras gene. Molecular Carcinogenesis 1988; 1: 161–170
   PubMedGoogle Scholar
• Brown K., Buchmann A., Balmain A. Carcinogen-induced mutations in the mouse c-Ha-ras gene provide evidence of multiple pathways for tumor progression. Proceedings of the National Academy of Sciences, USA 1990; 87: 538–542
   PubMed Web of Science ®Google Scholar
• Burvall K., Ridanpaa M., Husgafvel-Pursiainen K. Separation of transforming N-ras mutations in codons 12, 13 and 61 by denaturing gradient gel electrophoresis; investigations based on a set of phagemid constructs. Mutation Research 1993; 285: 287–295
   PubMedGoogle Scholar
• Cedar H. DNA methylation and gene activity. Cell 1988; 53: 3–4
   PubMed Web of Science ®Google Scholar
• Cohen J.B., Levinson A.D. A point mutation in the last intron responsible for increased expression and transforming activity of the c-Ha-ras oncogene. Nature 1988; 334: 119–224
   PubMed Web of Science ®Google Scholar
• Collins M., Myers R.M. Alteration in DNA helix stability due to base modification can be evaluated using denaturing gradient gel electrophoresis. Journal of Molecular Biology 1987; 198: 737–744
   PubMed Web of Science ®Google Scholar
• Feinberg A.P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 1983; 132: 5–13
   Google Scholar
• Fisher S.G., Lerman L.S. DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory. Proceedings of the National Academy of Sciences, USA 1983; 80: 1579–1583
   PubMed Web of Science ®Google Scholar
• Gray M. Detection of DNA sequence polymorphisms in human genomic DNA by using denaturing gradient gel blots. American Journal of Human Genetics 1992; 50: 331–346
   PubMed Web of Science ®Google Scholar
• Gray M., Charpentier A., Walsh K., Wu P., Bender W. Mapping point mutations in the Drosophila rosy locus using denaturing gradient gel blots. Genetics 1991; 127: 139–149
   PubMedGoogle Scholar
• Guerrero I., Villasante A., Corces V., Pellicer A. Activation of a c-Ki-ras oncogene by somatic mutation in mouse lymphomas induced by gamma-radiation. Science 1984; 225: 1159–1162
   PubMed Web of Science ®Google Scholar
• Jaffe D.R., Bowden G.T. Detection of distinct transforming genes in X-ray induced tumors. Carcinogenesis 1989; 10: 2243–2247
   PubMed Web of Science ®Google Scholar
• Jones P.A., Buckley J.D. The role of DNA methylation in cancer. Advances in Cancer Research 1990; 54: 1–23
   PubMedGoogle Scholar
• Krolewski B., Little J.B. Molecular analysis of DNA isolated from the different stages of X-ray induced transformation in vitro. Molecular Carcinogenesis 1989; 2: 27–33
   PubMed Web of Science ®Google Scholar
• Krolewski B., Little J.B. Application of denaturing gradient gel blots to detect p53 mutations in X-ray transformed mouse C3H 10T½ clones. Molecular Carcinogenesis 1993; 7: 190–196
   PubMedGoogle Scholar
• Krolewski A.S., Krolewski B., Gray M., Stanton V., Warram J.H., Housman D. High-frequency DNA sequence polymorphisms in the insulin receptor gene detected by denaturing gradient gel blots. Genomics 1992; 12: 705–709
   PubMed Web of Science ®Google Scholar
• Lerman L.S., Fisher S.G., Hurley I., Silverstein K., Lumelsky N. Sequence-determined DNA separations. Annual Review of Biophysics and Bioengineering 1984; 13: 399–423
   PubMedGoogle Scholar
• Leuthauser S.W.C., Thomas J.E., Guernsey D.L. Oncogenes in X-ray-transformed C3H 10T½ mouse cells and in X-ray induced mouse fibrosarcoma (RIF-1) cells. International Journal of Radiation Biology 1992; 62: 45–51
   PubMed Web of Science ®Google Scholar
• Myers R.M., Fisher S.G., Lerman L.S., Maniatis T. Nearly all single base substitutions in DNA fragments joined to GC-clamp can be detected by denaturing gradient gel electrophoresis. Nucleic Acids Research 1985; 13: 3131–3145
   PubMed Web of Science ®Google Scholar
• Ono T., Takahashi N., Okada S. Age-associated changes in DNA methylation and mRNA level of the c-myc gene in spleen and liver of mice. Mutation Research 1989; 219: 39–50
   PubMedGoogle Scholar
• Ramsden M., Cole G., Smith J., Balmain A. Differential methylation of the c-H-ras gene in normal mouse cells and during skin tumour progression. EMBO Journal 1985; 4: 1449–1454
   PubMedGoogle Scholar
• Reznikoff C.A., Bertram J.S., Brankow D.W., Heidelberger C. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Research 1983; 33: 3231–3238
   Google Scholar
• Ridanpaa M., Husgafvel-Pursiainen K. Denaturing gradient gel electrophoresis (DGGE) assay for K-ras and N-ras genes: detection of K-ras point mutations in human lung tumour DNA. Human Molecular Genetics 1993; 2: 639–644
   PubMedGoogle Scholar
• Sawey M.J., Hood A.T., Burns F.J., Garte S.J. Activation of c-myc and c-Ki-ras oncogenes in primary rat tumors induced by ionizing radiation. Molecular and Cellular Biology 1987; 7: 932–935
   PubMed Web of Science ®Google Scholar
• Sheffield V.C., Cox D.R., Lerman L.S., Myers R.M. Attachment of a 40 base pair G-C rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes. Proceedings of the National Academy of Sciences, USA 1989; 86: 232–236
   PubMed Web of Science ®Google Scholar
• Sloan S.R., Newcomb E.W., Pellicer A. Neutron radiation can activate K-ras via a point mutation in codon 146 and induces a different spectrum of ras mutations than does gamma radiation. Molecular and Cellular Biology 1990; 10: 405–408
   PubMed Web of Science ®Google Scholar
• Soman N.R., Wogan G.N. Activation of the c-Ki-ras oncogene in aflatoxin B1-induced hepatocellular carcinoma and adenoma in the rat. Detection by denaturing gradient gel electrophoresis. Proceedings of the National Academy of Sciences, USA 1993; 90: 2045–2049
   PubMed Web of Science ®Google Scholar
• Southern E.M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 1975; 98: 503–517
   PubMed Web of Science ®Google Scholar
• Stanton L.W., Watt R., Marcu K.B. Translocation, breakage and truncated transcripts of c-myc oncogene in murine plasmacytomas. Nature 1983; 303: 401–406
   PubMed Web of Science ®Google Scholar
• Terzaghi M., Little J.B. X-radiation induction of transformation in a C3H mouse embryo-derived cell line. Cancer Research 1976; 33: 1367–1374
   Google Scholar
• Traystman M.D., Higuchi M., Kasper C.K., Antonarakis S.E., Kazazian H. Use of denaturing gradient gel electrophoresis to detect point mutations in the factor VIII gene. Genomics 1990; 6: 293–301
   PubMedGoogle Scholar