Taxonomic investigation of Ralfsia-like (Ralfsiales, Phaeophyceae) taxa in the North Atlantic Ocean based on molecular and morphological data, with descriptions of Pseudoralfsiaceae fam. nov., Pseudoralfsia azorica gen. et sp. nov. and Nuchella vesicularis gen. et sp. nov.

References

• Areschoug, J.E. (1843). Algarum (phycearum) minus rite cognitarum pugillus secundus. Linnaea, 17: 257–269, pl. IX.
   Google Scholar
• Bittner, L., Payri, C.E., Couloux, A., Cruaud, C., Reviers, B. de. & Rousseau, F. (2008). Molecular phylogeny of the Dictyotales and their position within the brown algae, based on nuclear, plastidial and mitochondrial sequence data. Molecular Phylogenetics and Evolution, 49: 211–226.
   PubMed Web of Science ®Google Scholar
• Burrowes, R., Rousseau, F., Müller, D.G. & Reviers, B. de (2003). Taxonomic placement of Microzonia (Phaeophyceae) in Syringodermatales based on rbcL and 28S nrDNA sequences. Cryptogamie Algologie, 24: 63–73.
   Web of Science ®Google Scholar
• Caragnano, A., Foetisch, A., Maneveldt, G.W., Millet, L., Liu, L.-C., Lin, S.-M., Rodondi, G. & Payri, C.E. (2018). Revision of Corallinaceae (Corallinales, Rhodophyta): recognizing Dawsoniolithon gen. nov., Parvicellularium gen. nov. and Chamberlainoideae subfam. nov. containing Chamberlainium gen. nov. and Pneophyllum. Journal of Phycology, 54: 391–409.
   PubMed Web of Science ®Google Scholar
• Draisma, S.G.A., Prud’homme Van Reine, W.F. & Kawai, H. (2010). A revised classification of the Sphacelariales (Phaeophyceae) inferred from a psbC and rbcL based phylogeny. European Journal of Phycology, 45: 308–326.
   Web of Science ®Google Scholar
• Guiry, M.D. & Guiry, G.M. (2018). Algaebase. World-wide electronic publication, National University of Ireland, Galway (Online). Available from http://www.algaebase.org ( accessed 18 May 2018).
   Google Scholar
• Hind, K.R., Gabrielsen, P.W., Jensen, C.P. & Martone, P.T. (2016). Crusticorallina gen. nov., a nongeniculate genus in the subfamily Corallinoideae (Corallinales, Rhodophyta). Journal of Phycology, 52: 929–941.
   PubMed Web of Science ®Google Scholar
• Kain, J.M., Buchanan, J., Boo, S.M. & Lee, K.M. (2010). Colpomenia bullosa crust masquerading as Ralfsia verrucosa (Phaeophyceae) in southeast Australia. Phycologia, 49: 617–627.
   Web of Science ®Google Scholar
• Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Meintjes, P. & Drummond, A. (2012). Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28: 1647–1649.
   PubMed Web of Science ®Google Scholar
• Kogame, K., Horiguchi, T. & Masuda, M. (1999). Phylogeny of the order Scytosiphonales (Phaeophyceae) based on DNA sequences of rbcL, partial rbcS, and partial LSU nrDNA. Phycologia, 38: 496–502.
   Web of Science ®Google Scholar
• Lane, C.E., Mayes, C., Druehl, L.D. & Saunders, G.W. (2006). A multi-gene molecular investigation of the kelp (Laminariales, Phaeophyceae) supports substantial taxonomic re-organization. Journal of Phycology, 42: 493–512.
   Web of Science ®Google Scholar
• León-Alvarez, D., Reyes-Gómez, V.P., Wynne, M.J., Ponce-Márquez, M.E. & Quiróz-González, N. (2017). Morphological and molecular characterization of Hapalospongidion gelatinosum, Hapalospongidiaceae fam. nov. (Ralfsiales, Phaeophyceae) from Mexico. Botanica Marina, 60: 567–581.
   Web of Science ®Google Scholar
• Lim, P.-E., Sakaguchi, M., Hanyuda, T., Kogame, K., Phang, S.-M. & Kawai, H. (2007). Molecular phylogeny of crustose brown algae (Ralfsiales, Phaeophyceae) inferred from rbcL sequences resulting in the proposal for Neoralfsiaceae fam. nov. Phycologia, 46: 456–466.
   Web of Science ®Google Scholar
• McDevit, D.C. & Saunders, G.W. (2009). On the utility of DNA barcoding for species differentiation among brown macroalgae (Phaeophyceae) including a novel extraction protocol. Phycological Research, 57: 131–141.
   Web of Science ®Google Scholar
• McDevit, D.C. & Saunders, G.W. (2017). A molecular investigation of Canadian Scytosiphonaceae (Phaeophyceae) including descriptions of Planosiphon gen. nov. and Scytosiphon promiscuus sp. nov. Botany, 95: 653–671.
   Web of Science ®Google Scholar
• Nakamura, Y. (1972). A proposal on the classification of the Phaeophyta. In Contributions to the Systematics of Benthic Marine Algae of the North Pacific (Abbott, I.A. & Kurogi, M., editors), 147–155. Japanese Society of Phycology, Kobe.
   Google Scholar
• Parente, M.I. & Saunders, G.W. (2019). A molecular survey of Ralfsia sensu stricto (Ralfsiales, Phaeophyceae) in Canada uncovers three new species: R. robertii sp. nov., R. tenebris sp. nov. and R. unimaculata sp. nov. Botany. doi.org/10.1139/cjb-2018-0138.
   Google Scholar
• Poong, S.W., Lim, P.E., Phang, S.M., Gerung, G.S. & Kawai, H. (2013). Mesospora elongata sp. nov. (Ralfsiales, Phaeophyceae), a new crustose brown algal species from the Indo-Pacific region. Phycologia, 52: 74–81.
   Web of Science ®Google Scholar
• Poong, S.W., Lim, P.E., Phang, S.M., Sunarpi, H., West, J.A. & Kawai, H. (2014). A molecular-assisted floristic survey of crustose brown algae (Phaeophyceae) from Malaysia and Lombok Island, Indonesia based on rbcL and partial cox1 genes. Journal of Applied Phycology, 26: 1231–1242.
   Web of Science ®Google Scholar
• Poong, S.W., Lim, P.E., Phang, S.M., Sunarpi, H., West, J.A., Miller, K.A., Nelson, W. & Kawai, H. (2017). Two new species of Mesospora (Ralfsiales, Phaeophyceae) from the subtropical Indo-Pacific region. Phycologia, 56: 487–498.
   Web of Science ®Google Scholar
• Saunders, G.W. & McDevit, D.C. (2012). Methods for DNA barcoding photosynthetic protists emphasizing the macroalgae and diatoms. Methods in Molecular Biology, 858: 207–222.
   PubMedGoogle Scholar
• Saunders, G.W. & Moore, T.E. (2013). Refinements for the amplification and sequencing of red algal DNA barcode and RedToL phylogenetic markers: a summary of current primers, profiles and strategies. Algae, 28: 31–43.
   Web of Science ®Google Scholar
• Silberfeld, T., Leigh, J.W., Verbruggen, H., Cruaud, C., De Reviers, B. & Rousseau, F. (2010). A multi-locus time-calibrated phylogeny of the brown algae (Heterokonta, Ochrophyta, Phaeophyceae): investigating the evolutionary nature of the “brown algal crown radiation”. Molecular Phylogenetics and Evolution, 56: 659–674.
   PubMed Web of Science ®Google Scholar
• Stamatakis, A. (2014). RAxML version 8: a tool for phylogenetic analyses and post-analysis of large phylogenies. Bioinformatics, open access.
   Google Scholar