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British Journal of Biomedical Science Volume 68, 2011 - Issue 2
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Biomedical Science in Brief

Comparison of the identification of Acinetobacter spp. with API20NE and 16S rRNA gene sequencing techniques

A.J. MccarronNorthern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Belfast, Northern Ireland.;School of Biomedical Sciences, Centre for Molecular Biosciences, University of Ulster, Coleraine, Northern Ireland.
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J. XuNorthern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Belfast, Northern Ireland.;Department of Immunology and Pathogenic Biology, Molecular Bacteriology Laboratory, Key Laboratory of Environment and Genes related to Diseases of Chinese Ministry of Education, School of Medicine, Xi’an Jiaotong University, Xi’an, Shaanxi, People’s Republic of China, Northern Ireland.
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C. ArmstrongDepartment of Microbiology, Craigavon Area Hospital, Craigavon, Co. Armagh, Northern Ireland.
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G. GlynnDepartment of Microbiology,, Altnagelvin Hospital, Glenshane Road, Londonderry, Co. Londonderry, Northern Ireland.
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B. C. MillarNorthern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Belfast, Northern Ireland.
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R. B. McclurgNorthern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Belfast, Northern Ireland.
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L. HanDepartment of Immunology and Pathogenic Biology, Molecular Bacteriology Laboratory, Key Laboratory of Environment and Genes related to Diseases of Chinese Ministry of Education, School of Medicine, Xi’an Jiaotong University, Xi’an, Shaanxi, People’s Republic of China, Northern Ireland.
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C. E. GoldsmithNorthern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Belfast, Northern Ireland.
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P. J. RooneyNorthern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Belfast, Northern Ireland.
&
J. E. MooreNorthern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Belfast, Northern Ireland.;School of Biomedical Sciences, Centre for Molecular Biosciences, University of Ulster, Coleraine, Northern Ireland.Correspondence[email protected]
 show all
Pages 94-97 | Published online: 12 Feb 2018

References

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  • Ibrahim A, Gerner-Smidt P, Liesack W. Phylogenetic relationship of the twenty-one DNA groups of the genus Acinetobacter as revealed by 16S ribosomal DNA sequence analysis. Int J Syst Bacteriol 1997; 47 (3): 837–41.
  • Nemec A, De Baere T, Tjernberg I, Vaneechoutte M, van der Reijden TJK, Dijkshoorn L. Acinetobacter ursingii sp. nov. and Acinetobacter schindleri sp. nov., isolated from human clinical specimens. Int J Syst Evol Bacteriol 2001; 51 (Pt 5): 1891–9.
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  • Gundi VA, Dijkshoorn L, Burignat S, Raoult D, La Scola B. Validation of partial rpoB gene sequence analysis for the identification of clinically important and emerging Acinetobacter species. Microbiology 2009; 155 (Pt 7): 2333–41.
  • Millar BC, Xu J, Moore JE. Risk assessment models and contamination management: implications for broad-range ribosomal DNA PCR as a diagnostic tool in medical bacteriology. J Clin Microbiol 2002; 40 (5): 1575–80.
  • Jugo J, Kennedy R, Crowe MJ et al. Trends in bacteraemia on the haematology and oncology units of a UK tertiary referral hospital. J Hosp Infect 2002; 50 (1): 48–55.
  • Bosshard PP, Zbinden R, Abels S, Böddinghaus B, Altwegg M, Böttger EC. 16S rRNA gene sequencing versus the API 20 NE system and the VITEK 2 ID-GNB card for identification of nonfermenting Gram-negative bacteria in the clinical laboratory. J Clin Microbiol 2006; 44 (4): 1359–66.

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