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Original Articles

Production of Hybridoma Cell Lines Secreting Antibodies to Bovine Herpesvirus-1 Using Previously Frozen Splenocytes

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Pages 65-73 | Received 01 Nov 1997, Accepted 22 Jan 1998, Published online: 11 Nov 2011

References

  • Cianfriglia , M. , Armellini , D. , Massone , A. and Mariani , M. 1983 . Simple immunization protocol for high frequency production of soluble antigenspecific hybridomas . Hybridoma , 2 : 451 – 457 .
  • Kohler , G. and Milstein , C. 1975 . Continuous cultures of fused cells secreting antibody of predefined specificity . Nature (Lond.) , 256 : 495 – 497 .
  • Marusich , F. M. 1988 . Efficient hybridoma production using previously frozen splenocytes . J. Immunol. Methods , 114 : 155 – 159 .
  • Wagner , G. G. , Card , J. L. and Cowan , K. M. 1970 . Immunochemical studies of foot-and-mouth disease. VII. Characterization of foot-and-mouth disease virus concentrated by polyethylene glycol precipitation . Archiv. Ges. Virusforsch , 30 : 343 – 352 .

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