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Research Article

Oxidative DNA Damage: Biological Significance and Methods of Analysis

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Pages 331-457 | Published online: 29 Sep 2008

References

  • Tamir S, Tannenbaum SR. The role of nitric oxide (NO-) in the carcinogenic process. Biochim Biophys Acta 1996; 1288: F31–6.
  • Douki H, Cadet J. Peroxynitrite mediated oxidation of purine bases of nucleosides and isolated DNA. Free Radic Res 1996; 24: 369–80.
  • Spencer JPE, Wong J, Jenner A, et al. Base modifications and strand breakage in isolated calf thymus DNA and in DNA from human skin epidermal keratinocytes exposed to peroxynitrite or 3-morpholinosydnonimine. Chem Res Toxicol 1995; 9: 1152–58.
  • Douki T, Cadet J, Ames BN. An adduct between peroxynitrite and 2?-deoxyguanosine: 4,5-dihydro-5-hydroxy-4-(nitrosooxy)-2?-deoxyguanosine. Chem Res Toxicol 1996; 9: 3–7.
  • Ambs S, Hussain P, Harris CC. Interactive effects of NO and the p53 tumor suppressor gene in carcinogenesis and tumor progression. FASEB J 1997; 11: 443–8.
  • Wilson DM, Thompson LH. Life without DNA repair. Proc Natl Acad Sci USA 1997; 94: 12754–7.
  • Wennmalm A, Benthin G, Jungersten L, et al. Nitric oxide formation in man as reflected by plasma levels of nitrate, with special focus on kinetics, confounding factors and response to immunological challenge. In: Moncada S, Feelish M, Busse R, et al, eds. The Biology of Nitric Oxide. Vol. 4. Pp. 474–476. Portland Press, 1994.
  • Dallinga JW, Pachen DMFA, Lousberg AHP, et al. Volatile N-nitrosamines in gastric juice of patients with various conditions of the gastrointestinal tracts determined by gas chromatogra-phy-mass spectrometry and related to intragastric pH and nitrate and nitrite levels. Cancer Lett 1998; 124: 119–25.
  • Ohshima H, Bartsch H. Chronic infections and inflammatory processes as cancer risk factors: possible role of nitric oxide in carcinogenesis. Mutat Res 1994; 305: 253–64.
  • Totter JR. Spontaneous cancer and its possible relationship to oxygen metabolism. Proc Natl Acad Sci USA 1980; 77: 1763–7.
  • Ames BN. Endogenous oxidative DNA damage, aging, and cancer. Free Radic Res 1989; 7: 121–8.
  • Oshima H, Bartsch H. Chronic infections and inflammatory processes as cancer risk factors: possible role of nitric oxide in carcinogenesis. Mutat Res 1994; 305: 253–64.
  • Cerutti P. Oxy-radicals and cancer. Lancet 1994; 344: 862–3.
  • Feig DI, Reid TM, Loeb LA. Cancer Res (Suppl) 1994; 54: 1890s-4s.
  • Routledge MN, Wink DA, Keefer LK, et al. DNA sequence changes induced by two nitric oxide donor drugs in the supF assay. Chem Res Toxicol 1994; 7: 628–32.
  • Halliwell B, Gutteridge JMC. Free Radicals in Biology and Medicine. Oxford: Clarendon Press, 1989.
  • Kappus H. Lipid peroxidation: mechanisms, analysis, enzymology and biological relevance. In: Sies H, eds. Oxidative Stress. Pp 273–310. New York: Academic Press.
  • Cotgreave IA, Moldéus P, Orrenius S. Host biochemical defense mechanisms against prooxidants. Annu Rev Pharmacol Toxicol 1988; 28: 189–212.
  • Dizdaroglu M. Chemistry of free radical damage to DNA and nucleoproteins. In: Halliwell B, Aruoma OI, eds. DNA and Free Radicals. Pp 19–39. Chichester, West Sussex: Ellis Horwood Ltd, 1993.
  • Halliwell B, Aruoma OL. DNA damage by oxygen derived species. Its mechanism and measurement in mammalian systems. FEBS Lett 1991; 281: 9–19.
  • von Sonntag C. The Chemical Basis of Radiation Biology. Pp 116-166, 221-294. London: Taylor & Francis, 1987.
  • Steenken S. Purine bases, nucleosides and nucleotides: aqueous solution redox chemistry and transformation reactions of their radical cations and e- and OH adduct. Chem Rev 1989; 89: 503–20.
  • Epe B. In: Halliwell B, Aruoma OI, eds. DNA and Free Radicals. Pp 41-65. Chichester, West Sussex: Ellis Horwood Ltd, 1993.
  • Schreck R, Alberman K, Bauerle PA. Nuclear factor kappa B: an oxidative stress-responsive transcription factor of eukaryotic cells (a review). Free Radic Res Commun 1992; 17: 221–37.
  • Burdon RH. Superoxide and hydrogen peroxide in relation to mammalian cell proliferation. Free Radic Biol Med 1995; 18: 775–94.
  • Sarafian TA, Bredesen DE. Invited commentary: Is apoptosis mediated by reactive oxygen species? Free Radic Res 1994; 21: 1–8.
  • Jackson JH. Potential molecular mechanisms of oxidant-induced carcinogenesis. Environ Health Perspect (Suppl.) 1994; 102(Suppl. 10): 155–8.
  • Stevenson MA, Pollock SS, Coleman CN, et al. X-Irradiation, phorbol esters, and H2O2 stimulate mitogen-acitvated protein kinase activity in NIH-3T3 cells through the formation of reactive oxygen intermediates. Cancer Res 1994; 54:</b>12-5.
  • Lindahl T. DNA repair enzymes. Annu Rev Biochem 1982; 51: 61–87.
  • Pryor WA. Oxy-radicals and related species: their formation, lifetimes and reactions. Annu Rev Physiol 1986; 48: 657–67.
  • Weis SJ, Lobuglio AF. Biology of disease. Phagocyte generated oxygen metabolites and cellular injury. Lab Invest 1982; 47: 5–18.
  • Douki T, Martini R, Ravanat JL, et al. Measurement of 2,6-diamino-4-hydroxy-5-formamidopyrimidine and 8-oxo-7,8-dihydroguanine in isolated DNA exposed to gamma radiation in aqueous solution. Carcinogenesis 1997; 18: 2385–91.
  • Fridovich I. Biological effects of the superoxide radical. Arch Biochem Biophys 1986; 247: 1–11.
  • Oleinick NL, Chiu SM, Ramakrishnan N, et al. The formation, identification, and significantce of DNA-protein cross-links in mammalian cells. Br J Cancer Suppl 1987; 55(Suppl. VIII): 135–40.
  • Dizdaroglu M. Chemical determination of free radical-induced damage to DNA. Free Radic Biol Med 1991; 10: 225–42.
  • Krenkel K. Carcinogen-mediated oxidant formation and oxidative DNA damage. Pharmacol Ther 1992; 53: 127–166.
  • Dizdaroglu M, Holwitt E, Hagan MP, et al. Formation of cytosine glycol and 5,6-dihydroxycytosine in deoxyribonucleic acid on treatment with osmium tetroxide. Biochem J 1986; 235: 531–6.
  • Floyd RA, West MS, Eneff KL, et al. Methylene blue plus light mediates 8-hydroxy-guanine formation in DNA. Arch Biochem Biophys 1989; 273: 106–11.
  • Akman SA, Doroshow JH, Dizdaroglu M. Base modifications in plasmid DNA caused by potassium permanganate. Arch Biochem Biophys 1990; 282: 202–5.
  • Deeble DJ, Schuchmann MN, Steenken S, et al. Direct evidence for the formation of thymine radical cations form the reaction of (SO4)°− with thymine derivatives: a pulse radiolysis study with optical and conductance detection. J Phys Chem 1990; 94: 8186–92.
  • Angelov D, Berger M, Cadet J, et al. Comparison of the effects of high-power U.V.-laser pulses and ionizing radiation on nucleic acids and related compounds. Radiat Phys Chem 1991; 37: 717–27.
  • Boiteux S, Gajewski E, Laval J, et al. Substrate specificity of the Escherichia coli Fpg protein (formamidopyrimidine-DNA glycosylase): excision of purine lesions in DNA produced by ionizing radiation or photosensitization. Biochemistry 1992; 31: 106–10.
  • Sies H, Menck CF. Singlet oxygen induced DNA damage. Mutat Res 1992; 275: 367–75.
  • Box HC, Freund HG, Budzinski E, et al. Free radical-induced double base lesions. Radiat Res 1995; 141: 91–4.
  • Floyd RA, Watson JJ, Wong PK, et al. Hydroxyl free radical adduct of deoxyguanosine: sensitive detection and mechanisms of formation. Free Radic Res Commun 1986; 1: 163–72.
  • Ames BN. Endogenous oxidative DNA damage, aging and cancer. Free Rad Res Commun 1989; 7: 121–8.
  • Dizdaroglu M. In: Aruoma OI and Halliwell B, eds. DNA and Free Radical: Techniques, Mechanisms and Applications. Pp 3–26. St. Lucia: OICA International Press, 1998.
  • Musarrat J, Wani AA. Quantitative immunoanalysis of promutagenic 8-hydroxy-2'-deoxyguanosine in oxidized DNA. Carcinogenesis 1994; 15: 2037–43.
  • Shigenaga MK, Hagen TM, Ames BN. Oxidative damage and mitochondrial decay in aging. Proc Natl Acad Sci USA 1994; 91: 10771–8.
  • Harman D. Free radical theory of aging. Mutat Res 1992; 275: 257–66.
  • Ambrosio G, Zweirer JL, Duilio G, et al. Evidence that mitochondrial respiration is a source of potentially toxic oxygen radicals in intact rabbit hearts subjected to ischemia and reflow. J Biol Chem 1993; 268: 18532–41.
  • Guidot DM, McCord JM, Wright R, et al. Absence of electron transport (Rho° State) restores growth of a manganese-superoxide dismutase-deficient Saccharmyces cerevisiae in hyperoxia. J Biol Chem 1993; 268: 26699–703.
  • Totter JR. Spontanous cancer and its possible relationship to oxygen metabolism. Proc Natl Acad Sci USA 1980; 77: 1763–7.
  • Cheeseman K., In: Halliwell B and Aruoma OI, eds. DNA and Free Radicals. Pp 109–144. Ellis Horwood, Chichester.
  • Beckman KB, Ames BN. Oxidative DNA damage: assesing its role in cancers and aging. In: Aruoma OI, Halliwell B, eds. DNA and Free Radicals: Techniques, Mechanisms and Applications. St. Lucia: OICA International Press, 1998.
  • Suter M, Richter C. Fragmented mitochondrial DNA is the predominant carrier of oxidized DNA bases. Biochemistry 1999; 38: 459–64.
  • Arnheim N, Cortopassi G. Deleterious mitochondrial DNA mutations accumulate in aging human tissues. Mutat Res 1992; 275: 157–67.
  • Mecocci P, MacGarvey U, Beal MF. Oxidative damage to mitochondrial DNA is increased in Alzheimer’s disease. Ann Neural 1994; 36: 747–51.
  • Nackerdien Z, Olinski R, Dizdaroglu M. DNA base damage in chromatin of y-irradiated cultured human cells. Free Rad Res Commun 1992; 16: 259–73.
  • Candeis LP, Patel KB, Stratford MRL, et al. Free hydroxyl radicals are formed on reaction between the neutrophil-derived species superoxide andion and hypochlorus acid. FEBS Lett 1993; 333: 151–3.
  • Huie RE, Padmaja S. The reaction of NO with superoxide. Free Radic Res Commun 1993; 18: 195–9.
  • Padmaja S, Hui RE. Reaction of nitric oxide with peroxyl radicals. Biochem Biophys Res Commun 1993; 195: 539–44.
  • Beckman JS, Chen J, Ischeropoulos H, et al. Oxidative chemistry of peroxynitrite. Method Enzymol 1994; 233: 229–40.
  • Yermilov V, Rubio J, Becchi M, et al. Formation of 8-nitroguanine by the reaction of guanine with peroxynitrite in vitro. Carcinogenesis 1995; 16: 2045–50.
  • Nascimento ALTO, Meneghini R. Cells transfected with transferrin receptor cDNA lacking the iron regulatory domain become more sensitive to the DNA-damaging action of oxidative stress. Carcinogenesis 1995; 16: 1335–8.
  • Flint DH, Smyk-Randall E, Tuminello JF, et al. The inactivation of dihydroxy-acid dehydratase in Escherichia coli treated with hyperbaric oxygen occurs because of the destruction of its Fe-S cluster, but the enzyme remains in the cell in a form that can be reactivated. J Biol Chem 1993; 268: 25547–52.
  • Halliwell B, Gutteridge JMC. The antioxidants of human extracellular fluids. Arch Biochem Biophys 1990; 280: 1–8.
  • Bolann BJ, Ulvik RJ. On the limited ability of superoxide to release iron from ferritin. Eur J Biochem 1990; 193: 899–904.
  • Gutteridge JMC. Iron promoters of the Fenton reaction and lipid peroxidation can be released from haemoglobin by peroxides. FEBS Lett 1986; 201: 291–5.
  • Swain JA, Darley-Usmar V, Gutteridge JMC. Peroxynitrite releases copper from caeruloplasmin: implications for atherosclerosis. FEBS Lett 1994; 342: 49–52.
  • Li Y, Trush MA, Yager JD. DNA damage caused by reactive oxygen species originating from a copper-dependent oxidation of the 2-hydroxy catechol of estradiol. Carcinogenesis 1994; 15: 1421–7.
  • Li Y, Trush MA. Reactive oxygen-dependent DNA damage resulting from the oxidation of phenolic compounds by a copper-redox cycle mechanism. Cancer Res 1994; 54(7 Suppl.): 1895s-8s.
  • Spencer JPE, Jenner A, Aruoma OI, et al. Intense oxidative DNA damage promoted by L-dopa and its metabolites. Implications for neurodegenerative disease. FEBS Lett 1994; 353: 246–50.
  • Halliwell B. How to characterize a biological antioxidant. Free Radic Res Commun 1990; 9: 1–32.
  • Nakayama T. Suppression of hydroperoxide-induced cytotoxicity by polyphenols. Cancer Res 1994; 54(7 Suppl.): 1991s-3s.
  • Weitzman SA, Gordon LI. Inflammation and cancer: role of phagocyte-generated oxidants in carcinogenesis. Blood 1990; 76: 655–63.
  • Zimmerman R, Cerutti P. Active oxygen acts as a promoter of transformation in mouse embryo C3H/10T1/2/C18 fibroblasts. Proc Natl Acad Sci USA 1984; 81: 2085–7.
  • Craven PA, Pfanstiel J, Rubertis FR. Role of reactive oxygen in bile salt stimulation of colonic epithelial proliferation. J Clin Invest 1986; 77: 850–9.
  • Nicotera TM, Privalle C, Wang TC, et al. Differential proliferative responses of Syrian hamster embryo fibroblasts to paraquat-generated superoxide radicals depending on tumor suppressor gene function. Cancer Res 1994; 54: 3884–8.
  • Jacobson MD, Raff MC. Programmed cell death and Bcl-2 protection in very low oxygen. Nature 1995; 374(6525): 814–6.
  • Slater AFG, Nobel CSI, Maellaro E, et al. Nitrone spin traps and a nitroxide antioxidant inhibit a common pathway ofthymocyte apoptosis. Bioch J 1995; 306: 771–8.
  • Buttke TM, Sandstrom PA. Redox regulation of programmed cell death in lymphocytes. Free Radic Res 1995; 22: 389–97.
  • Hockenbery DM, Oltvai ZN, Yin XM, et al. Bcl-2 functions in an antioxidant pathway to prevent apoptosis. Cell 1993; 75: 241–51.
  • Haldar S, Negrini M, Monne M, et al. Down-regulation of bcl-2 by p53 in breast cancer cells. Cancer Res 1994; 54: 2095–7.
  • Reid TM, Feig DI, Loeb LA. Mutagenesis by metal-induced oxygen radicals. Environ Health Perspect 1994; 102: 57–61.
  • Geiegerstanger BH, Kagawa TF, Chen SL, et al. Base-specific binding of copper(II) to Z-DNA. The 1.3-A single crystal structure of d(m5CGUAm5CG) in the presence of CuCl2. J Biol Chem 1991; 266: 20185–91.
  • Basu AK, Loechler EL, Leadon SA, et al. Genetic effects of thymine glycol: site-specific mutagenesis and molecular modeling studies. Proc Natl Acad Sci USA 1989; 86: 7677–81.
  • Kamiya H, Ueda T, Ohgi T, et al. Misincorporation of dAMP opposite 2-hydroxyadenine, an oxidative form of adenine. Nucl Acid Res 1995; 23: 761–6.
  • Evans J, Maccabee M, Hatahet Z, et al. Thymine ring saturation and fragmentation products: lesion bypass, misinsertion and implications for mutagenesis. Mutat Res 1993; 299: 147–56.
  • Maccabee M, Evans JS, Glackin MP, et al. Pyrimidine ring fragmentation products. Effects of lesion structure and sequence context on mutagenesis. J Biol Chem 1994; 236: 514–530.
  • Van Den Akker E, Lutgerink JT, Lafleur MV, et al. The formation of one-G deletions as a consequence of single-oxygen-induced DNA damage. Muta Res 1994; 309: 45–52.
  • de Oliveira CR, Ribeiro DT, Nigro RG., et al. Singlet oxygen induced mutation spectrum in mammalian cells. Nucl Acids Res 1992; 20: 4319–23.
  • Ribeiro DT, de Oliveira CR, Di Mascio P. et al. Singlet oxygen induces predominantly G to T transversions on a single-stranded shuttle vector replicated in monkey cells. Free Radic Res 1994; 21: 75–83.
  • Purmal AA, Kow YW, Wallace SS. Major oxidative products of cytosine, 5-hydroxycytosine and 5-hydroxyuracil, exhibit sequence context-dependent mispairing in vitro. Nucl Acid Res 1994; 22: 72–8.
  • Feig DI, Loeb LA. Mechanisms of mutation by oxidative DNA damage: reduced fidelity of mammalian DNA polymerase ?. Biochemistry 1993; 32: 4466–73.
  • Hussain SP, Aguilar F, Amstad P, et al. Oxy-radical induced mutagenesis of hotspot codons 248 and 249 of the human p53 gene. Oncogene 1994; 9: 2277–81.
  • Stadtman ER. Oxidation of free amino acids and amino acid residues in proteins by radiolysis and by metal-catalyzed reactions. Annu Rev Biochem 1993; 62: 797–821.
  • Halliwell B. Albumin, an important extracellular antioxidant? Biochem Pharmacol 1988; 37: 569–71.
  • Halliwell B, Gutteridge JMC. Oxygen-free radicals and iron in relation to biology and medicine: some problems and concepts. Arch Biochem Biophys 1986; 246: 501–14.
  • Aruoma OI, Halliwell B. Superoxide-dependant and ascorbate dependant fromation of hy-droxyl radicals from hydrogen peroxide in the presence of iron. Are lactoferrin and transferrin promotors of hydroxy-radical generation? Biochem J 1987; 241: 273–8.
  • Grootveld M, Bell JD, Halliwell B, et al. Non-transferin-bound iron in plasma or serum from patients with idiopathic hemchromatosis. Characterization by high performance liquid chro-matography and nuclear magnetic resonance spectroscopy. J Biol Chem 1989; 264: 4417–22.
  • McLaren GD, Muir WA, Kellermeyer RW. Iron overload disorders: natural history, pathogen-esis, diagnosis and therapy. Crit Rev Clin Lab Sci 1983; 19: 205–66.
  • Halliwell B. How to characterize a biologic antioxidant? Free Radic Res Commun 1990; 9: 1–32.
  • Esterbauer H, Striegl G, Puhl H, et al. Continuous monitoring of in vitro oxidation of human low density lipoprotein. Free Radic Res Commun 1989; 6: 67–75.
  • Wefers H, Sies H. The protection of ascorbate and glutathione against microsomal lipid peroxidation is dependent on vitamin E. Eur J Biochem 1988; 174: 353–7.
  • Halliwell B. Vitamin C: antioxidant of pro-oxidant in vivo? Free Radic Res 1996; 25: 439–54.
  • Laughton MJ, Evans PJ, Moroney MA, et al. Inhibition of mammalian 5-lipoxygenase and cyclooxygenase by flavonoids and phenolic dietary additives. Biochem Pharmacol 1991; 42: 1673–81.
  • Hodnick WF, Kung FS, Roettger WJ, et al. Inhibition of mitochondrial respiration and production of toxic oxygen radicals by flavonoids. Biochem Pharmacol 1986; 35: 2345–57.
  • Bendich A, Machlin LJ, Scandurra O, et al. The antioxidant role of vitamin C. Free Radic Biol Med 1986; 2: 419–44.
  • Rice-Evans CA, Sampson J, Bramley PM, et al. Why do we expect carotenoids to be antioxidant in vivo? Free Radic Res 1997; 26: 381–98.
  • Rowe PM. Beta-carotene takes a collective beating. Lancet 1996; 347: 249.
  • Halliwell B. Oxidative stress, nutrition and health. Experimental strategies for optimization of nutritional antioxidant intake in humans. Free Radic Res 1996; 25: 57–74.
  • Von Poppel G, Poulsen H, Loft S, et al. No influence of ?-carotene on oxidative DNA damage in male smokers. J Natl Cancer Inst 1995; 87: 310–1.
  • Verhagen V, Poulsen HE, Loft S, et al. Reduction of oxidative DNA damage in humans by Brussels sprouts. Carcinogenesis 1995; 16: 969–70.
  • French M, Dreosti I, Aitken C. Vitamin E supplements and their effect on vitamin-E status in blood and genetic damage rates in peripheral blood lymphocytes. Carcinogenesis 1997; 18: 359–64.
  • Pool-Zobel BL, Bub A, Müller H, et al. Consumption of vegetables reduces genetic damage in humans: first results of a human intervention trial with carotenoid-rich foods. Carcinogen-esis 1997; 18: 1847–50.
  • Priemé H, Loft S, Nyyssönen K, et al. No effect of supplementation with vitamin E, as ascorbic acid or coenzyme Q10 on oxidative DNA damage estimated by 8-oxo-7,8-deoxyguanosine excretion in smokers. Am J Clin Nutr 1997; 65: 503–7.
  • Halliwell B. Drug antioxidant effects. A basis for drug selection? Drugs 1991; 42: 569–605.
  • Wiseman H. Tamoxifen: molecular basis of use in cancer treatment and prevention. Chichester: John Wiley and Sons, 1994.
  • Demple B, Harrison L. Repair of oxidative damage to DNA: enzymology and biology. Annu Rev Biochem 1994; 63: 915–48.
  • Adachi S, Zeizig M, Möller L. Improvements in the analytical method for 8-hydroxydeoxyguanosine in nuclear DNA. Carcinogenesis 1995; 16: 253–8.
  • Malins DC, Haimanot R. Major alterations in the nucleotide structure of DNA in cancer of the female breast. Cancer Res 1991; 51: 5430–2.
  • Jaruga P, Zastawny TH, Skokowksi J, et al. Oxidative DNA damage and antioxidant enzyme activities in human lung cancer. FEBS Lett 1994; 341: 59–64.
  • Bashir S, Harris G, Denman MA, et al. Oxidative DNA damage and cellular sensitivity to oxidative stress in human autoimmune diseases. Ann Rheum Dis 1993; 52: 659–66.
  • Hagen TM, Huang S, Curnutte J, et al. Extensive oxidative DNA damage in hepatocytes of transgenic mice with chronic active hepatitis destined to develop hepatocellular carcinoma. Proc Natl Acad Sci USA 1994; 91: 12808–12.
  • Olinski R, Zastawny TH, Fokinski M., et al. DNA base modifications and antioxidant enzyme activities in human benign prostatic hyperplasia. Free Radic Biol Med 1995; 18: 807–13.
  • Satoh MS, Piorier GG, Lindahl T. NAD(+)-dependent repair of damaged DNA by human cell extracts. J Biol Chem 1993; 268: 5480–7.
  • Satoh MS, Lindahl T. Enzymatic repair of oxidative DNA damage. Cancer Research 1994; 54(7 Suppl.): 1899s-901s.
  • Satoh MS, Jones CJ, Wood RD, et al. DNA excision-repair defect of xeroderma pigmentosum prevents removal of a class of oxygen free radical-induced base lesions. Proc Natl Acad Sci USA 1993; 90: 6335–9.
  • Marx J. DNA repair comes into its own. Science 1994; 266: 728–30.
  • Haring M, Rudiger H, Demple B, et al. Recognition of oxidized bases abasic sides by repair endonucleases. Nucl Acids Res 1994; 22: 2010–5.
  • Hatahet Z, Kow YW, Purmal AA, et al. New substrates for old enzymes. 5-Hydroxy-2?-deoxycytidine and 5-hydroxy-2?-deoxyuridine are substrates for Escherichia coli endonu-clease III and formamidopyrimidine DNA N-glycosylase, while 5-hydroxy-2?-deoxyuridine is a substrate for uracil DNA N-glycosylase. J Biol Chem 1994; 269: 18814–20.
  • Dizdaroglu M, Laval J, Boiteux S. Substrate specificity of the Escherichia coli endonuclease III: excision of thymine- and cytosine-derived lesions in DNA produced by radiation-generated free radicals. Biochemistry 1993; 32: 12105–11.
  • Spencer JPE, Jenner A, Chimel K, et al. DNA strand breakage and base modification induced by hydrogen peroxide treatment of human respiratory tract epithelial cells. FEBS Lett 1995; 374: 233–6.
  • Floyd RA. The role of 8-hydroxyguanine in carcinogenesis. Carcinogenesis 1990; 11: 1447–50.
  • Epe B. Genotoxicity of singlet O2. Chem Biol Interact 1992; 80: 239–60.
  • Kasai H. Analysis of a form of oxidative DNA damage, 8-hydroxy-2?-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis. Mutat Res 1997; 387: 147–63.
  • Hellbock HJ, Beckman KB, Shigenaga MK, et al. DNA oxidation matters: the HPLC-electrochemical detection assay of 8-oxo-deoxyguanosine and 8-oxo-guanine. Proc Natl Acad Sci USA 1998; 95: 288–93.
  • Dizdaroglu M. Gas chromatography-mass spectrometry of free radical-induced product of pyrimidines and purines in DNA. Methods Enzymol 1990; 193: 842–57.
  • Hartwig A. Assesment of oxidative DNA damage by frequency of formamidopyrimidine-glycosylase (FPG) sensitive DNA lesions. In: Aruoma OI, Halliwell B, eds. DNA and Free Radicals. Techniques, Mechanisms and Applications. St. Lucia: OICA International Press, 1998.
  • Nackerdien Z, Rao G, Cacciuttolo MA, et al. Chemical nature of DNA-protein cross-links produced in mammalian chromatin by hydrogen peroxide in the presence of iron or copper ions. Biochemistry 1991; 30: 4872–9.
  • Savva R, McAuly-Hecht K, Brown T, et al. The structural basis of specific base-excision repair by uracil-DNA glycosylase. Nature 1995; 373: 487–93.
  • Bridges BA. MutY “directs” mutation? Nature 1995; 375(6534): 741.
  • Mo JY, Maki H, Sekiguchi M. Hydrolytic elimination of a mutagenic nucleotide, 8-oxodGTP, by human 18-kilodalton protein: sanitization of nucleotide pool. Proc Natl Acad Sci USA 1992; 89: 11021–5.
  • Roldan-Arjona T, Wei YF, Carter KC, et al. Molecular cloning and functional expression of human cDNA encoding the antitumor enzyme 8-hydroxyguanine-DNA glycosylase. Proc Natl Acad Sci USA 1997; 94: 8016–20.
  • Wood RD. Nucleotide excision repair in mammalian cells. J Biol Chem 1997; 272: 23465–8.
  • Poirier MC, Weston A. Human DNA adduct measurements: state of the art. Environ Health Perspect 1996; 104: 883–93.
  • Bartsch H. DNA adducts in human carcinogenesis: ethiological relevance and structure-acitivity relationship. Mutat Res 1996; 340: 67–9.
  • Cadet J, D’Ham C, Douki T, et al. Facts and artifacts in the measurement of oxidative base damage to DNA. Free Radic Res 1998; 29: 541–50.
  • Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants and the degenerative diseases of aging. Proc Natl Acad Sci USA 1993; 90: 7915–22.
  • Malins DC, Haimanot R. Major alterations in the nucleotide structure of DNA in cancer of the female breast. Cancer Res 1989; 49: 5489–96.
  • Wiseman H, Halliwell B. Damage to DNA by reactive oxygen and nitrogen species, role in inflammatory disease and progression to cancer. Biochem J 1996; 313: 17–29.
  • Loeb LA. Endogenous carcinogens: molecular oncology into the 21st century. Cancer Res 1989; 49: 5489–96.
  • Burdon RH, Alliangana D, Gill V. Endogenously generated active oxygen species and cellular glutahione levels in relation to BHK-21 cell proliferation. Free Radic Res 1994; 21: 121–34.
  • Chaudhary AK, Nobuko M, Reddy GR, et al. Detection of endogenous malondialdehyde-deoxyguanosine adducts in human liver. Science 1994; 265: 1580–2.
  • El Ghissassi F, Barbin A, Nair J, et al. Formation of 1, N 6-ethenoadenine and 3, N 4-ethenocytosine by lipid peroxidation products and nucleic acid bases. Chem Res Toxicol 1995; 8: 278–83.
  • Douki T, Ames BN. An HPLC-EC assay for 1, N 2-propano adducts of 2'-deoxyguanosine with 4-hydroxynonenal and other a,(3-unsatured aldehydes. Chem Res Toxicol 1994; 7: 511–8.
  • Taguchi T, Ohashi M. Changes in fidelity levels of DNA polymerases oc-1, a-2 and (3 during aging in rats. Mech Ageing Dev 1997; 99: 33–47.
  • Dizdaroglu M. Mechanisms of free radical damage to DNA. In: Aruoma OI, Halliwell B, eds. DNA and Free Radicals: Techniques, Mechanisms and Applications. Pp 1–24. Saint Lucia, OICA International, 1998.
  • Beckman KB, Ames BN. Oxidative decay of DNA. J Biol Chem 1997; 272: 19633–6.
  • Collins A, Cadet J, Epe B, et al. Problems in the measurement of 8-oxoguanine in human DNA. Report of a workshop, DNA oxidation, held in Aberdeen, UK, 19-21 January, 1997. Carcinogenesis 1997; 18: 1833–6.
  • Dizdaroglu M. Oxidative damage to DNA in mammalian chromatin. Mutat Res 1992; 275: 331–42.
  • Cadet J, Weinfeld M. Detecting DNA damage. Anal Chem 1993; 65: 675A-82A.
  • Epe B. DNA damage profiles induced by oxidizing agents. Rev Physiol Biochem Pharmacol 1996; 127: 223–49.
  • Collins AR, Dobson VL, Dusinska M, et al. The comet assay: what can it really tell us? Mutat Res 1997; 375: 183–95.
  • Kvam E, Tyrrell RM. Artificial background and induced levels of oxidative base damage in DNA from human cells. Carcinogenesis 1997; 18(11): 2281–83.
  • Floyd RA, Watson JJ, Harris J, et al. Formation of 8-hydroxydeoxyguanosine, hydroxyl free radical adducts of DNA in granulocytes exposed to the tumor promotor, tetra-decanoylphorbolacetate. Biochem Biophys Res Commun 1986; 137: 841–6.
  • Halliwell B, Dizdarogly M. Commentary: he measurement of oxidative damage to DNA by HPLC and GC/MS techniques. Free Radic Res Commun 1992; 16(2): 75–87.
  • Kaur H, Halliwell B. Measurement of oxidized and methylated DNA bases by HPLC with electrochemical detection. Biochemistry 1996; 318: 21–3.
  • Finnegan MT, Herbert KE, Evans MD, et al. Development of an assay to measure 8-oxogunanine using HPLC with electrochemical detection. Biochem Soc Trans 1995; 23(3): 431S.
  • Herbert KE, Evans MD, Finnegan MT, et al. A novel HPLC procedure for the analysis of 8-oxoguanine in DNA. Free Radic Biol Med 1996; 20: 467–73.
  • Germadnik D, Pilger A, Rüdiger HW. Assay for the determination of urinary-2'-deoxyguanosine by high-performance liquid chromatography with electrochemical detection. J Chromatogr B 1996; 689: 399–403.
  • Dizdaroglu M. The use of capillary gas chromatography-mass spectrometry for identification of radiation-induced base damage and DNA-base amino-acid cross links. J Chromatogr 1984; 295: 103–21.
  • Dizdaroglu M. Quantitative determination of oxidative base damage in DNA by stable isotope-dilution mass spectrometry. FEBS Lett 1993; 315: 1–6.
  • Dizdaroglu M. Chemical determination of oxidative DNA damage by gas chromatography-mass spectrometry. Method Enzymol 1994; 234: 3–16.
  • Dizdaroglu M. Application of capillary gas chromatography-mass spectrometry to chemical characterization of radiation-induced base damage in DNA: implication for assessing DNA repair processes. Anal Biochem 1985; 144: 593–603.
  • Nackerdien Z, Olinski R, Dizdaroglu M. DNA base damage in chromatin of y-irradiated cultured human cells. Free Radic Res Commun 1992; 16: 259–73.
  • Poulsen HE, Weimann A, Loft S. Methods to detect DNA damage by free radicals: relation to exercise. Proc Nutr Soc 1999; 58: 1007–14.
  • Cadet J, Berger M, Douki T, et al. Effects of UV and visible radiation on DNA-final base damage. Biol Chem 1997; 378: 1275–86.
  • Pflaum M, Will O, Epe B. Determination of steady-state levels of oxidative DNA base modifications in mammalian cells by means of repair eneonucleases. Carcinogenesis 1997; 18: 2225–31.
  • Hayakawa M, Torri K, Sugiyama S, et al. Age-associated accumulation of 8-hydroxydeoxyguanosine in mitochondrial DNA of human diaphragm. Biochem Biophys Res Commun 1991; 179: 1023–9.
  • Ravanat JL, Duretz B, Guiller A, et al. Isotope dilution high-performance liquid chromatography-electrospray tandem mass spectrometry assay for the measurement of 8-oxo-7,8-dihydro-2?-deoxyguanosine in biological samples. J Chromatogr B 1998; 715: 349–56.
  • Hayakawa M, Ogawa T, Sugiyama S, et al. Massive conversion of guanosine to 8-hydroxy-guanosine in mouse liver mitochondrial DNA by administration of azidothymidine. Biochem Biophys Res Commun 1991; 176: 87–93.
  • Serrano J, Palmeira MC, Wallace KB, et al. Determination of 8-hydroxydeoxyguanosine in biological tissue by liquid chromatography/electrospray ionization-mass spectrometry/mass spectrometry. Rapid Commun Mass Spectrom 1996; 10: 1789–91.
  • Rosier J, Van Peteghem C. Detection of oxidatively modified 2?-deoxyguanosine-3?-mono-phosphate, using 32P-postlabeling and anionexchange thin-layer chromatography. J Chroma-togr 1988; 434: 222–7.
  • Lu LJ, Tasaka F, Hokanson JA, et al. Detection of 8-hydroxy-2?-deoxyguanosine in deoxyri-bonucleic acid by the 32P-postlabeling method. Chem Pharm Bull 1991; 39: 1880–2.
  • Lutgerink JT, de Graaf E, Hoebee B, et al. Detection of 8-hydroxyguanine in small amounts of DNA by 32P-postlabeling. Anal Biochem 1992; 201: 127–33.
  • Wilson VL, Taffe BG, Shields PG, et al. Detection and quantification of 8-hydroxydeoxyguanosine adducts in perepheral blood of people exposed to ionizing radiation. Environ Health Perspect 1993; 99: 261–63.
  • Cadet J, Incardona MF, Odin F, et al. Measurement of oxidative base damage to DNA by using HPLC-32P-postlabeling and GC/MS-selective ion monitoring assays. IARC Sci Publ 1993; 124: 271–76.
  • Povey AC, Wilson VL, Weston A, et al. Detection of oxidative damage by 32P-postlabeling: 8-hydroxydeoxyguanosine as a marker of exposure. IARC Sci Publ 1993; 124: 105–14.
  • Schuler D, Otteneder M, Sagelsdorff P, et al. Comparative analysis of 8-oxo-2?-deoxyguanosine in DNA by 32P-postlabeling and 33P-postlabeling and electrochemical detection. Carcinogen-esis 1997; 18: 2367–71.
  • Podmore K, Farmer PB, Herbert KE, et al. 32P-postlabeling approaches for the detection of 8-oxo-2?-deoxyguanosine-3?-monophophate in DNA. Mutat Res 1997; 178: 139–49.
  • Sharma M, Box HC, Paul CR. Detection and quantification of 8-hydroxdeoxyguanosine-5?-monophosphate in X-irradiated calf-thymus DNA by fluorescence postlabeling. Biochem Biophys Res Commun 1990; 167: 416–24.
  • Sharma M, Box HC, Kelman DJ. Fluoreschence postlabeling assay of cis-thymidine glycol monophosphate in X-irradiated calf-thymus DNA. Chem Biol Interact 1990; 74: 107–17.
  • Frenkel K, Zhong Z, Wei H, et al. Quantitative high-performance liquid chromatography analysis of DNA oxidized in vitro and in vivo. Anal Biochem 1991; 196: 126–36.
  • Bhimani RS, Troll W, Grunberger F, et al. Inhibition of oxidative stress in HeLa cells by chemopreventive agents. Cancer Res 1993; 53: 4528–33.
  • Ashok BT, Ahmed J, Ali R. Immunochemical detection of oxidative DNA damage in cancer and aging using anti-reactive oxygen species modified DNA monoclonal antibody. Int J Biochem Cell Biol 1998; 30: 1367–77.
  • Toyokuni S, Tanaka T, Hattori Y, et al. Quantitative immunohistochemical determination of 8-hydroxy-2?-deoxyguanosine by a monoclonal antibody N45.1: its application to ferric Nitriloacetate-induced renal carcinogenesis. Lab Invest 1997; 76: 365–74.
  • Dusinska M, Collins A. Detection of oxidized purines and UV-induced photoproducts in DNA single cells, by inclusion of lesion-specific enzymes in the comet assay. ATLA 1996; 24: 405–11.
  • Epe B, Hegler J. Oxidative DNA damage: endonuclease fingerprint. Method Enzymol 1994; 234: 122–31.
  • Hartwig A, Dally H, Schiepegrell R. Sensitive analysis of oxidative DNA damage in mammalian cells: use of the bacterial Fpg protein in combination with alkaline unwinding. Toxicology 1996; 110: 1–6.
  • Loft S, Poulsen H.E. Cancer risk and oxidative DNA damage in man. J Mol Med 1996; 74: 297–312.
  • Helbock HJ, Beckman KB, Shigenage MK, et al. DNA oxidation matters: HPLC-electro-chemical detection assay of 8-oxo-deoxyguanosine and 8-oxo-guanine. Proc Natl Acad Sci USA 1998; 95: 288–93.
  • Bianchini F, Donato F, Faure H, et al. Urinary excretion of 5-(hydroxymethyl)uracil in healthy volunteers: effects of active and passive tabocco smoke. Int J Cancer 1998; 77: 40–6.
  • Hong YC, Park HS, Ha EH. Influence of genetic susceptibility on the urinary excretion of 8-hydroxydeoxyguanosine of firefighters. Occupational and Environmental Medicine 2000; 57: 370–5.
  • Radak Z, Pucsuk J, Boros S, et al. Changes in urine 8-hydroxydeoxyguanosine levels of super marathon runners during a four-day race period. Life Sci 2000; 66: 1763–7.
  • Yin B, Whyatt RM, Perera FP, et al. Determination of 8-hydroxydeoxyguanosine by immunoaffinity chromatography-monoclonal antibody-bases ELISA. Free Radic Res 1995; 18: 1023–32.
  • Faure H, Incardona MF, Boujet C, et al. Gas chromatographic-mass spectrometric determination of 5-hydroxymethyluracil in human urine by stable isotope dilution. J Chromatogr B 1993; 616: 1–7.
  • Faure H, Mousseau M, Cadet J, et al. Urine 8-oxo-7,8-dihydro-2?-deoxyguanosine vs. 5-(hydroxymethyl)-uracil as DNA oxidation marker in adriamycin-treated patients. Free Radic Res 1998; 28: 377–83.
  • Podmore ID, Griffiths HR, Herbert KE, et al. Vitamin C exhibits both a prooxidant and antioxidant behavior in vivo. Nature 1998; 392(6676): 559.
  • Rehman A, Collis CS, Yand M, et al. The effect of iron and vitamin C co-supplementation on oxidative damage to DNA in healthy volunteers. Biochem Biophys Res Commun 1998; 246: 293–8.
  • Malins DC, Polissar NL, Gunselman SJ. Progression of human breast cancers to the metastatic state is linked to hydroxyl radical-induced DNA damage. Proc Natl Acad Sci USA 1996; 93: 2557–63.
  • Sent¸rker S, Dizdaroglu M. The effect of experimental conditions on the levels of oxidatively modified bases in DNA as measured by gas chromatography-mass spectrometry: how many modified bases are involved? Prepurification or not? Free Radic Biol Med 1999; 27: 370–80.
  • Schram K. Preparation of trimethylsilyl derivatives of nucleic acid components for analysis by mass spectrometry. Method Enzymol 1990; 193: 792–5.
  • Watson JT. Selected-ion measurements. Method Enzymol 1990; 193: 86–106.
  • England TG, Jenner A, Aruoma OI, et al. Determination of oxidative DNA base damage by gas chromatography-mass spectrometry. Effect of derivatization conditions on artifactual formation of certain base oxidation products. Free Radic Res 1998; 29: 321–30.
  • Malins DC, Haimanot R. The etiology of cancer: hydroxyl radical-induced DNA lesions in histologically normal livers of fish from a population with liver tumours. Aquatic Toxicol 1991; 20: 123–30.
  • Malins DC, Haimanot R. 4,6-diamino-5-formamidopyrimidine, 8-hydroxyguanin and 8-hydroxyadenine in DNA form neoplastic liver of English Sole exposed to carcinogens. Biochem Biophys Res Commun 1990; 173: 614–9.
  • Dunn BP, Black JJ, Maccubbin A. 32P-postlabeling analysis of aromatic DNA adducts in fish from polluted areas. Cancer Res 1987; 47: 6543–8.
  • Mee LK, Adelstein SJ. Predominance of core histones in formation of DNA—protein crosslinks in gamma-irradiated chromatin. Proc Natl Acad Sci USA 1981; 78: 2194–8.
  • Gajewski E, Rao G, Nackerdien Z, et al. Modifications of DNA bases in mammalian chroma-tin by radiation-generated free radical. Biochemistry 1990; 29: 7876–82.
  • Burton K. Determination of DNA concentrations with diphenylamine. Method Enzymol 1968; 12: 163–6.
  • Smith PK, Krohn RI, Hermanson GT, et al. Measurement of protein using bicinchoninic acid. Anal Biochem 1985; 150: 76–85.
  • Claycamp HG. Phenol sensitization of DNA to subsequent oxidative damage in 8-hydroxyguanosine assays. Carcinogenesis 1992; 13: 1289–92.
  • Nakajima M, Takeuchi T, Morimoto K. Determination of 8-hydroxydeoxyguanosine in human cells under oxygen-free conditions. Carcinogenesis 1996; 17: 787–91.
  • Harris G, Bashir S, Winyard PG. 7,8-dihydro-8-oxo-2?-deoxyguanosine present in DNA is not simply an artifact of isolation. Carcinogenesis 1994; 15: 411–3.
  • Dizdaroglu M. Facts about the artifacts in the measurement of oxidative DNA base damage by gas chromatography-mass spectrometry. Free Radic Res 1998; 29: 551–63.
  • Jenner A, England TG, Aruoma IO, et al. Measurement of oxidative DNA damage by gas chromatography-mass spectrometry: ethanethiol prevents artifactual generation of oxidized DNA bases. Biochem J 1998; 331: 365–9.
  • Holmberg I, Stal P, Hamberg M. Quantitative determination of 8-hydroxy-2?-deoxyguanosine in human urine by isotope dilution mass spectrometry: normal levels in hemochromatosis. Free Radic Biol Med 1999; 26: 129–35.
  • Spencer JP, Whiteman M, Jenner A, et al. Nitrite-induced deamination and hypochlorite-induced oxidation of DNA in intact human respiratory tract epithelial cells. Free Radic Biol Med 2000; 28: 1039–50.
  • Matsui A, Ikeda T, Enomoto K, et al. Increased formation of oxidative DNA damage, 8-hydroxy-2?-deoxyguanosine, in human breast cancer tissue and its relationship to GSTP1 and COMT genotypes. Cancer Lett 2000; 151: 87–95.
  • Honda M, Yamada Y, Tomonaga M, et al. Correlation of urinary 8-hydroxy-2?-deoxyguanosine, a biomarker of oxidative DNA damage, and clinical features of hematological disorders: a pilot study. Leuk Res 2000; 24: 461–8.
  • Loft S, Fischer-Nielsen A, Jeding IB, et al. 8-Hydroxy-2?-deoxyguanosine as an urinary biomarker of oxidative DNA damage. J Toxicol Environ Health 1993; 40: 391–404.
  • Tarng DC, Huang TP, Liu TY, et al. Effect of vitamin E-bonded membrane on the 8-hydroxy-2?-deoxyguanosine level in leukocyte DNA of hemodialysis patients. Kidney Int 2000; 58: 790–9.
  • Loft S, Vistisen K, Ewertz M, et al. Oxidative DNA damage estimated by 8-hydroxdeoxyguanosine excretion in man: influence of smoking, gender and body mass index. Carcinogenesis 1992; 13: 2241–7.
  • Loft S, Poulsen HE. Markers of oxidative damage to DNA: antioxidants and molecular damage. Method Enzymol 1998; 300: 166–84.
  • Poulsen HE, Weimann A, Loft S. Urinary measurement of 8-oxodG (8-oxo-2?-deoxyguanosine). In: Lunec J, eds. Handbook of Clinical Analysis: In Vivo Damage to Biomolecules. London: John Wiley and Sons Ltd, 1999.
  • Berger M, Anselmino C, Mouret JF, et al. High-performance liquid chromatography-electro-chemical assay for monitoring the formation of 8-oxo-7,8-dihydroadenine and its related 2?-deoxyribonucleoside. J Liquid Chromatogr 1990; 13: 929–40.
  • Wagner JR, Hu CC, Ames BN. Endogenous oxidative damage of deoxycytidine in DNA. Proc Natl Acad Sci USA 1992; 89: 3380–4.
  • Hofer T, Möller L. Reduction of oxidation during the preparation of DNA and analysis of 8-hydroxy-2?-deoxyguanosine. Chem Res Toxicol 1998; 11: 882–7.
  • Beland FA, Dooley KL, Casciano DA. Rapid isolation of carcinogen-bound DNA and RNA by hydroxyapatite chromatography. J Chromatogr 1979; 174: 177–86.
  • Crain PF. Preparation and enzymatic hydrolysis of DNA and RNA for mass spectrometry. Methods Enzymol 1990; 193: 782–90.
  • Takeuchi T, Morimoto K. Crocidolite asbestos increased 8-hydroxydeoxyguanosine levels in cellular DNA of a human promyelocytic leukemia cell line, HL60. Carcinogenesis 1994; 73: 1825–35.
  • Ravanat JL, Gremaud E, Markovic J, et al. Detection of 8-oxoguanine in cellular DNA using 2,6-diamino-8-oxopurine as an internal standard for high-performance liquid chromatography with electrochemical detection. Anal Biochem 1998; 260: 30–7.
  • Takeuchi T, Nakajima M, Ohto Y, et al. Evalutation of 8-hydroxydeoxyguanosine, a typical oxidative DNA damage, in human leukocytes. Carcinogenesis 1994; 15: 1519–23.
  • Shen HM, Chia SE, Ong CN. Evalutation of oxidative DNA damage in human sperm and its association with male infertility. J Androl 1999; 20: 718–23.
  • ESCODD (European Standards Committee on Oxidative DNA damage). Comparison of different methods of measuring 8-oxoguanine as a marker of oxidative DNA damage. Free Rad Res 2000; 32: 333–41.
  • Cadet J, Douki T, Ravanat JL. Artifacts associated with the measurement of oxidatized DNA bases. Environ Health Perspect 1997; 105: 1034–39.
  • Bogdanov MB, Beal MF, McCabe DR, et al. A carbon column-based liquid chromatography electrochemical approach to routine 8-hydroxy-2?-deoxyguanosine measurement in urine and other biologic matrices: a one-year evalution of methods. Free Radic Biol Med 1999; 27: 647–66.
  • Thier R, Brüning T, Kocher K, et al. Determination of urinary thymidine glycol using affinity chromatography, HPLC and post-column reaction detection. Arch Toxicol 1999; 73: 479–84.
  • Tagesson C, Kallberg M, Klintenberg C, et al. Determination of urinary 8-hydroxyde-oxyguanosine by automated coupled-column high performance liquid chromatography: a powerful technique for assaying in vivo oxidative DNA damage in cancer patients. Eur J Cancer 1995; 31A: 934–40.
  • Ravanat JL, Guicherd P, Tuce Z, et al. Simultaneous determination of five DNA lesions in human urine. Chem Res Toxicol 1998; 12: 802–8.
  • Berger M, Cadet J, Berube R, et al. Reversed-phase high-performance liquid chromatography-thermospray mass spectrometry of radiation-induced decomposition products of thymine and thymidine. J Chromatgr 1992; 593: 133–8.
  • Smith RD, Loo JA, Edmonds CG, et al. New developments in biochemical mass spectrometry: electrospray ionization. Anal Chem 1990; 62: 882–99.
  • Nelson VC. Synthesis of isotopically labelled DNA degradation products for use in mass spectrometric studies of cellular DNA damage. J Label Compounds Radiopharm 1996; 38: 713–24.
  • LaFrancois CJ, Fujimoto J, Sowers LC. Synthesis and characterization of isotopically enriched pyrimidine deoxynucleoside oxidation damage products. Chem Res Toxicol 1998; 11: 75–83.
  • Stadler RH, Staempfli AA, Fay LB, et al. Synthesis of multiply-labeled [15N3,13C1]-8-oxo-substituted purine bases and their corresponding 2?-deoxynucleosides. Chem Res Toxicol 1994; 7: 784–91.
  • Ravanat JL, Turesky RJ, Gremaud E, et al. Determination of 8-oxoguanine in DNA by gas chromatography-mass spectrometry and HPLC-electrochemical detection: overestimation of the background level of the oxidized base by the gas chromatography-mass spectrometry assay. Chem Res Toxicol 1995; 8: 1039–45.
  • Hamberg M, Zhang LY. Quantitative determination of 8-hydroxyguanine and guanine by isotope dilution mass spectrometry. Anal Biochem 1995; 229: 336–44.
  • Douki T, Delatour T, Bianchini F, et al. Observation and prevention of an artefactual formation of oxidized DNA bases and nucleosides in the GC-EIMS method. Carcinogenesis 1996; 17: 347–53.
  • Dalakas MC, Illa I, Pezeshkpour GH, et al. Mitochondrial myopathy caused by long-term zidovudine therapy. N Engl J Med 1990; 322: 1098–105.
  • Téoule R. Radiation-induced DNA damage and its repair. Int J Radiat Biol 1987; 51: 573–89.
  • Potier N, Van Dorselaer A, Codrier Y, et al. Negative electrospray ionization mass spectrom-etry of synthetic and chemically modified oligonucleotides. Nucl Acid Res 1994; 22: 3895–903.
  • Reddy DM, Rieger RA, Torres MC, et al. Analysis of synthetic oligodeoxynucleotides containing modified components by electrospray ionization mass spectrometry. Anal Biochem 1994; 220: 200–7.
  • M¸ller M, Belas FJ, Blair JA, et al. Analysis of 1, N2-ethenoguanine and 5,6,7,9-tetrahydro-7-hydroxy-9-oxoimidazo[1,2-a]purine in DNA treated with 2-chlorooxirane by high-performance liquid chromatography/electrospray mass spectrometry and comparison of amounts to other DNA adducts. Chem Res Toxicol 1997; 10: 242–7.
  • Richling E, Herderich M, Schreire P. High-performance liquid chromatography: electrospray tandem mass spectrometry (HPLC-ESI-MS-MS) for the analysis of heterocyclic aromatic amines (HAA). Chromatographia 1996; 42: 7–11.
  • Reddy DM, Iden CR. Analysis of modified deoxynucleosides by electrospray ionization mass spectrometry. Nucleos Nucleot 1993; 12: 815–26.
  • Yamaguchi R, Hirano T, Asami S, et al. Increased 8-hydroxyguanine levels in DNA and its repair activity in rat kidney after administration of a renal carcinogen, ferric nitrilotriacetate. Carcinogenesis 1996; 17: 2419–22.
  • Douki T, Berger M, Raoul S, et al. Determination of 8-oxo-purines in DNA using amperometric detection. In: Favier AE, Cadet J, Kalyanaraman B, Fontecave M, Pierre JL, eds. Analysis of Free Radicals in Biological Systems. Pp 213–224. Basel: Birkhauser Verlag, 1995.
  • Dizdaroglu M., Jaruga P, Rodriguez H. Measurement of 8-hydroxy-2?-deoxyguanosine in DNA by highperformance liquid chromatography-mass spectrometry: comparison with measurement by gas chromatography-mass spectrometry. Nucl Acid Res 2001; 29: E12.
  • Rodriguez H, Jurado J, Laval J, et al. Comparison of the levels of 8-hydroxyguanine in DNA as measured by gas chromatography-mass spectrometry following hydrolysis of DNA by Escherichia coli Fpg protein or formic acid. Nucl Acid Res 2000; 28: E75.
  • Cadet J, Odin F, Mouret JF, et al. Chemical and biochemical postlabeling methods for singling out specific oxidative DNA lesions. Mutat Res 1992; 275: 343–54.
  • Randerath K, Reddy MY, Gupta RC. 32P-postlabeling test for DNA damage. Proc Natl Acad Sci USA 1981; 78: 6126–9.
  • Devanaboyina U, Gupta RC. Sensitive detection of 8-hydroxy-2?-deoxyguanosine in DNA 32P-postlabeling assay and the basal levels in rat tissues. Carcinogenesis 1996; 17: 917–24.
  • Gupta RC, Reddy MV, Randerath K. 32P-postlabeling analysis of non-radioactive aromatic carcinogen-DNA adducts. Carcinogenesis 1982; 3: 1081–92.
  • Reddy MV, Gupta RC, Randerath E, et al. 32P-postlabeling test for covalent DNA binding of chemicals in vivo: application to a variety of aromatic carcinogens and methylating agents. Carcinogenesis 1984; 5: 231–43.
  • Möller L, Hofer T. [32P]ATP mediates formation of 8-hydroxy-2?-deoxyguanosine from 2?-deoxyguanosine, a possible problem in the 32P-postlabeling assay. Carcinogenesis 1997; 18: 2415–19.
  • Randerath K, Zhou GD, Monk SC, et al. Enhanced levels in neonatal rat liver of 7,8-dihydro-8-oxo-2?-deoxyguanosine (8-hydroxydeoxyguanosine), a major mutagenic oxidativ DNA lesion. Carcinogenesis 1997; 18: 1419–21.
  • Oakley GG, Devanaboyina U, Robertson LW, et al. Oxidative DNA damage induced by activation of polychlorinated biphenyls (PCBs): implication for PCH-induced oxidative stress in breast cancer. Chem Res Toxicol 1996; 9: 1285–92.
  • Aruoma OL, Halliwell B, Gajewski E, et al. Copper-ion dependent damage to the bases in DNA in the presence of hydrogen peroxide. Biochem J 1991; 273: 601–4.
  • Wang YJ, Ho YS, Lo MJ, et al. Oxidative modification of DNA bases in rat liver and lung during chemical carcinogenesis and aging. Chem Biol Interact 1995; 94: 135–45.
  • Shimoda R, Nagashima M, Sakamoto M, et al. Increased formation of oxidative DNA damage, 8-hydroxydeoxyguanosine, in human livers with chronic hepatitis. Cancer Res 1994; 54: 3171–2.
  • Kasai H, Nishimura S, Kurokawa Y, et al. Oral administration of the renal carcinogen, potassium bromate, specifically produces 8-hydroxydeoxyguanosine in rat target organ DNA. Carcinogenesis 1987; 8: 1959–61.
  • Cattley RC, Glover SE. Elevated 8-hydroxydeoxyguanosine in hepatic DNA of rats following exposure to peroxisome proliferators: relationship to carcinogenesis and nuclear localization. Carcinogenesis 1993; 14: 2495–9.
  • Cadet J, Bianchini F, Girault I, et al. Measurement of base damage to DNA by the use of HPLC/32-postlabeling, immunological and noninvasive assays. In: Aruoma OI, Halliwell B, eds. DNA and Free Radicals: Techniques, Mechanisms and Applications. Saint Lucia, OICA International.
  • Gupta RC, Arif JM. A TLC enrichment-mediated 32P-postlabeling assay for the sensitive detection of 8-oxodeoxyguanosine (8-oxodG) in human tissues. Proc Amer Assoc Cancer Res 1998; 39: abstract 1960.
  • Moller L, Zeisig M, Vodicka P. Optimization of and HPLC method for analysis of 32P-postlabelled DNA adducts. Carcinogenesis 1993; 14: 1343–8.
  • Zeisig M, Moller L. 32P-postlabeling high performance liquid chromatographic improvements to characterize DNA adducts steriomers from benzo[a]pyrene and benzo[c]phenantrene, and to separate DNA adducts form 7,12-dimethylbenz[a]anthrene. J Chromatogr B 1997; 691: 341–50.
  • Mouret JF, Odin F, Polverelli M, et al. 32P-postlabeling measurement of adenine N-1-oxide in cellular DNA exposed to hydrogen peroxide. Chem Res Toxicol 1990; 3: 102–10.
  • Thompson PA, Kadlubar FF, Vena SM, et al. Exfoliated ductal epithelial cells in human breast milk: a source of target tissue DNA for molecular epidemiologic studies of breast cancer. Cancer Epidemiol Biomark Prev 1998; 7: 37–42.
  • Jorgenson JW, Lukacs KD. Zone electrophoresis in open-tubular glass capillaries. Anal Chem 1981; 53: 1298.
  • Terabe S, Otsuka K, Ichikawa K, et al. Electrokinetic separations with micellar solutions and open-tubular capillaries. Anal Chem 1984; 56: 111–3.
  • Zhao Z, Fuciarelli AF, Smith RD. Identification and characterization of radiation damaged products of monophosphate nucleosides by on-line capillary isotachophoresis-electrospray ionization mass spectrometry. J Capil Electrophoresis 1996; 3: 111–6.
  • Cheng YF, Dovichi NJ. Subattomole amino acid analysis by capillary zone electrophoresis and laser-induced fluorescence. Science 1988; 242(4878): 562–4.
  • Olefirowicz TM, Ewing AG. Capillary electrophoresis in 2 an 5 µm diameter capillaries: application tot cytoplasmic analysis. Anal Chem 1990; 62: 1872–6.
  • Tsuda T, Nakagawa G, Sato M, et al. Separation of nucleotides by high-voltage capillary electrophoresis. J Appl Biochem 1983; 5: 330–6.
  • Grune T, Ross GA, Schmidt H, et al. Optimized separation of purine bases and nucleosides in human cord plasma by capillary zone electrophoresis. J Chromatogr 1993; 636: 105–11.
  • Suzuki T, Yamada M, Furukawa H, et al. Detection of 2?-deoxyoxanosine by capillary electrophoresis. Nucleic Acids Symp Ser 1997; 37: 239–40.
  • Suzuki T, Yamaoka R, Nishi M, et al. J Am Chem Soc 1996; 118: 2515–2516.
  • Xue Q, Yeung ES. Differences in the chemical reactivity of individual molecules of an enzyme. Nature 1995; 373(6516): 681–3.
  • Craig DB, Wong JCY, Dovichi N.J. Detection of attomolar concentrations of alkaline phos-phatase by capillary electrophoresis using laser-induced fluorescence detection. Anal Chem 1996; 68: 697–700.
  • Mank AJG, Velthorst NH, Brinkman UATh, et al. Near-infrared laser-induced fluorescence detection in column liquid chromatography. A comparison of various lasers and detection systems I. Continuous wave lasers. J Chromatogr A 1995; 695: 165–74.
  • Mank AJG., Velthorst NH, Brinkman UATh. Et al. Near-Infrared laser-induced fluorescence detection in column liquid chromatography. A comparison of various lasers and detection systems. II. Pulsed lasers. J Chromatogr A 1995; 695: 175–84.
  • Le XC, Xing JZ, Lee J, et al. Inducible repair of thymine glycol detected by an ultrasensitive assay for DNA damage. Science 1998; 280: 1066–9.
  • Marples B, Skov KA. Small doses of high-linear energy transfer radiation increase the radioresistance of Chinese hamster V79 cells to subsequent X irradiation. Rad Res 1996; 146: 382–7.
  • Smidth RD, Wahl JH, Goodlett DR, et al. Capillary electrophoresis/mass spectrometry. Anal Chem 1993; 65: 574A-84A.
  • Bocek P, Deml M, Gebauer P, et al. Analytical Isotachophoresis. Pp 1–10. New York: VCH, 1988.
  • Bocek P, Deml M, Gebauer P, et al. Analytical Isotachophoresis. Pp 187–192. New York: VCH, 1988.
  • Bocek P, Deml M, Gebauer P, et al. Analytical Isotachophoresis. Pp 1–10. New York: VCH, 1988.
  • McLuckey SA, Habibi-Goudarzi S. Ion-trap tandem mass-spectrometry applied to small multiply-charged oligonucleotides with a modified base. J Am Soc Mass Spectrom 1994; 5: 740–7.
  • Pflaum M, Kielbassa C, Garmyn M, et al. Oxidative DNA damage induced by visible light in mammalian cells: extent, inhibition by antioxidants and genotoxic effects. Mutat Res 1998; 408: 137–46.
  • Collins AR, Ai-guo M, Duthie SJ. The kinetics of repair of oxidative DNA damage (strand breaks ans oxidised pyrimidines) in human cells. Mutat Res 1995; 336: 69–77.
  • Deng XS, Tuo J, Poulsen HE, et al. 2-Nitropropane-induced DNA damage in rat bone marrow. Mutat Res 1997; 391: 165–9.
  • Collins AR, Dobson VL, Dusinska M, et al. The comet assay: what can it really tell us? Mutat Res 1997; 375: 183–93.
  • Ross GM, McMillan TJ, Wilcox P, et al. The single cell microgel electrophoresis asay (comet assay): thechnical aspects and applications. Report of the 5th LH Gray Trust Workshop, Institute of Cancer Research. Mutat Res 1994; 337: 57–60.
  • Tuo J, Loft S, Thomsen MS, et al. Ex vivo time-dependent cell DNA-degradation shown by single cell gell electrophoresis. Pharmacol Toxicol 1996; 78: 55–7.
  • Collins AR, Dusinska M, Gedik CM, et al. Oxidative DNA damage: do we have a reliable biomarker? Environ Health Perspect 1996; 104: 465–9.
  • Pouget JP, Ravanat JL, Douki T, et al. Measurements of DNA base damage in cells exposed to low doses of gamma radiation: comparison between the HPLC-EC and the comet assays. Int J Rad Biol 1999; 75: 51–8.
  • Sauvaigo S, Serres C, Signorini N, et al. Use of the single cell gel electrophoresis assay for the immunofluorescent detection of a specific DNA damage. Anal Biochem 1998; 259: 1–7.
  • Tchou J, Kasai H, Shibutani S, et al. 8-oxoguanine (8-hydroxyguanine) DNA glycosylase and its substrate specificity. Proc Natl Acad Sci USA 1991; 88: 4690–4.
  • Kasten U, Beyersmann D, Dahm-Daphi J, et al. Sensitive nonradioactive detection of UV-induced cyclobutane pyrimidine dimers in intact mammalian cells. Mutat Res 1995; 336: 143–52.
  • Föhe C, Dikomey E. Induction and repair of DNA base damage studied in X-irradiated CHO cells using the M. luteus extract. Int J Rad Biol 1994; 66: 697–704.
  • Lagorio S, Tagesson C, Forastiere F, et al. Exposure to benzene and urinary concentrations of 8-hydroxydeoxyguanosine, a biological marker of oxidative DNA damage to DNA. Occup Environ Med 1994; 51: 739–43.
  • Cheng KC, Cahill DS, Kasai H, et al. 8-Hydroxydeoxyguanosine, an abundant form of oxidative DNA damage causes G-T and A-T substitutions. J Biol Chem 1992; 267: 166–72.
  • Moller L, Hofer T, Zeisig M. Methodological considerations and factors affecting 8-hydroxy-2-deoxyguanosine analysis. Free Radic Res 1998; 29: 511–24.
  • Hegler J, Bittner D, Boiteux S, et al. Quantification of oxidative DNA modifications in mitochondria. Carcinogenesis 1993; 14: 2309–12.
  • Nagayama T, Lan J, Henshall DC, et al. Induction of oxidative DNA damage in the peri-infarct region after permanent focal cerebral ischemia. J Neurochem 2000; 75: 1716–28.
  • Lan J, Henshall DC, Simon RP, et al. Formation of the base modification 8-hydroxy-2?-deoxyguanosine and DNA fragmentation following seizures induced by systemic kainic acid in rat. J Neurochem 2000; 74: 302–9.
  • Yarborough A, Zhang YL, Hsu TM, et al. Immunoperoxidase detection of 8-hydroxyguanosine in aflatoxin B1-treated rat liver and human mucosal cells. Cancer Res 1996; 56: 683–8.
  • Osawa T, Yoashida A, Kawakishi S, et al. Protective role of dietary antioxidants in oxidative stress. In: Cutler RG, Packer L, Bertram J. et al, eds. Oxidative Stress and Aging. Pp 367–377. Basel: Berkhauser Verlag.
  • Haegele AD, Gillette C, O’Neill C, et al. Plasma xanthophyll carotenoids correlelate inversely with indices of oxidative DNA damage and lipid peroxidation. Cancer Epidemiol Biomark Prev 2000; 9: 421–5.
  • Witherell HL, Hiatt RA, Replogle M, et al. Helicobacter pylori infection and urinary excretion of 8-hydroxy-2?-deoxyguanosine, an oxidative DNA adduct. Cancer Epidemiol Biomark Prev 1998; 7: 91–6.
  • Degan P, Shigenaga MK, Park EM, et al. Immunoaffinity isolation of urinary 8-hydroxy-2?-deoxyguanosine and 8-hydroxyguanine and quantitation of 8-hydroxy-2?-deoxyguanosine in DNA by polyclonal antibodies. Carcinogenesis 1991; 12: 865–71.
  • Sattler U, Calsou P, Boiteux S, et al. Detection of oxidative base DNA damage by a new biochemical assay. Arch Biochem Biophys 2000; 376: 26–33.
  • Salles B, Sattler U, Bozzato C, et al. Repair of oxidative DNA damage in vitro: a tool for screening antioxidative compounds. Food Chem Toxicol 1999; 37: 1009–14.
  • Kawashima M, Bando T, Nakamura T, et al. Cytoprotective effects of nitroglycerine in ishemia-reperfusion-induced lung injury. Am J Respir Crit Care Med 2000; 161: 935–43.
  • Won MH, Kang TC, Jeon GS, et al. Immunohistochemical detection of oxidative DNA damage induced by ischemia-reperfusion insults in gerbil hippocampus in vivo. Brain Res 1999; 836: 70–8.
  • Xu A, Wu LJ, Santella RM, et al. Role of oxyracials in mutagenicity and DNA damage induced by crocidolite asbestos in mammalian cells. Cancer Res 199; 59: 5922–6.
  • Toyokuni S, Iwasa Y, Kondo S, et al. Intranuclear ditribution of 8-hydroxy-2?-deoxyguanosine: an immunocytochemical study. J Histochem Cytochem 1999; 47: 833–5.
  • Erhola M, Toyokuni S, Okada K, et al. Biomarker evidence of DNA oxidation in lung cancer patients: association of urinary 8-hydroxy-2?-deoxyguanosine excretion with radiotherapy, chemotherapy, and response to treatment. FEBS Lett 1997; 409: 287–91.
  • Kato I, Vogelman JH, Dilman V, et al. Effect of supplementation with chromium picolinate on antibody titers to 5-hydroxymethyluracil. Eur J Epidemiol 1998; 14: 621–6.
  • Frenkel K, Karkoszka J, Cohen B, et al. Occupational exposures to Cd, Ni, and Cr modulate titers of antioxidized DNA base autoantibodies. Env Health Perspect 1994; 102: 221–5.
  • Frenkel K, Karkoszka J, Kim E, et al. Recognition of oxidized DNA bases by sera of patients with inflammatory diseases. Free Radic Biol Med 1993; 14: 483–94.
  • Frenkel K, Khasak D, Karkoszka J, et al. Enhanced antibody titers to an oxidized DNA base in inflammatory and neoplastic diseases. Exp Dermatol 1992; 1: 242–7.
  • Kohler G, Milstein C. Continuous cultures of fused cells secreting antibodies of predicted specificity. Nature 1975; 256: 495–7.
  • Hsu SM, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 1981; 29: 577–80.
  • Provot C, Bozzato C, Salles B. A simple chemiluminescence microplate assay (the 3D assay) to study antioxidizing compounds. Mutat Res 1997; 379: S168.
  • Kelman DJ, Lilga KT, Sharma M. Synthesis and application of fluorescent labeled nucleotides to assay DNA damage. Chem Biol Interact 1988; 66: 85–100.
  • Azadnia A, Campbell R, Sharma M. The scope of dansyl vs fluorescein label in fluorescence postlabeling assay for DNA damage. Anal Biochem 1994; 218: 444–8.
  • Edkins TJ, Shelly DC. Capillary liquid chromatography of dansylated nucleotide using laser fluorescence detection. Personal communication to Sharma M., Dept. of Chemistry and Chem. Eng., Stevens Institute of Technology, Hoboken, NJ.
  • Lee T, Yeung ES, Sharma M. Micellar electrokinetic capillary chromatographic separation and laser-induced fluorescence detection of 2?-deoxynucleoside-5?-monophosphates of normal and modified bases. J Chromatogr 1991; 565: 197–206.
  • Waschke S, Reefschlager J, Barwolf D, et al. 5-hydroxymethyluracil-2?-deoxyuridine, a normal constituent in certain Bacillus subtilis phages, is cytotoxic for mammalian cells. Nature 1975; 255: 629–30.
  • Shirnamé-Moré L, Rossman TG, Troll W, et al. Genetic effects of 5-hydroxymethyl-2?-deoxyuridine, a product of ionizing radiations. Mutat Res 1987; 178: 177–86.
  • Tchou J, Grollman AP. Repair of DNA containing the oxidatively-damaged base, 8-oxoguanine. Mutat Res 1993; 299: 277–87.
  • Maeda H, Katsuki T, Akaike T, et al. High correlation between lipid peroxide radical and tumor promotion effect: suppression of tumor promotion in the Epstein-Barr virus/B-lympho-cyte system and scavenging of alkyl peroxide radicals by various vegetable extracts. Jpn J Cancer Res 1992; 83: 923–8.
  • Loft S, Poulsen HE. Cancer risk and oxidative DNA damage in man. J Mol Med 1996; 74: 297–312.
  • Park EM, Shinenaga MK, Degan P, et al. Assay of excised oxidative DNA lesions: Isolation of 8-oxoguanine and its nucleoside derivatives from biological fluids with a monoclonal antibody column. Proc Natl Acad Sci USA 1992; 88: 3375–9.
  • Bianchini F, Hall J, Donato F, et al. Monitoring urinary excretion of 5-hydroxymethyluracil for assessment or oxidative DNA damage and repair. Biomarkers 1996; 1: 178–84.
  • Poulsen HE, Loft S, Prieme H, et al. Oxidative DNA damage in vivo: relationship to age, plasma antioxidants, drug metabolism, glutathione-S-transferase activity and urinary creatine excretion. Free Radic Res 1998; 29: 565–71.
  • Djuric Z, Heilbrum LK, Reading BA, et al. Effects of a low-fat diet on levels of oxidative damage to DNA in human peripheral nucleoted blood cells. J Natl Cancer Inst 1991; 83: 766–9.
  • Olinski R, Zastawny T, Budzbon J, et al. DNA base modifications in chromatin of human cancerous tissues. FEBS Lett 1992; 309: 193–8.
  • Malins DM. Identification of hydroxyl-radical-induced lesions in DNA base structure: biomarkers with a putative link to cancer development. J Toxicol Environ Health 1993; 40: 247–61.
  • Halliwell B. Oxygen and nitrogen are pro-carcinogens. Damage to DNA by reactive oxygen, chlorine and nitrogen species: measurement, mechanisme and effects of nutrition. Mutat Res 1999; 443: 37–52.
  • Halliwell B. Oxygen and nitrogen are pro-carcinogens. Damage to DNA by reactive oxygen, chlorine and nitrogen species: measurement, mechanisms and effects of nutrion. Mutat Res 1999; 443: 37–52.
  • Prieme H, Loft S, Cutler RG, et al. Measurement of oxidative DNA injury in humans; evalution of commercially available ELISA assays. In: Kumpulainen JT, eds. Natural Antioxi-dants and Food Quality in Artherosclerosis and Cancer Prevention. Pp 78–82. London: The Royal Society of Chemistry.
  • Dizdaroglu M, Bergtold DS. Characterization of free radical-induced base damage in DNA at biological relevant levels. Anal Biochem 1986; 156: 182–8.
  • Abalea V, Cillard J, Dubois MP, et al. Iron-induced oxidative DNA damage and its repair in primary rat hepatocyte culture. Carcinogenesis 1998; 19: 1053–9.
  • Cadet J, Bardet M, Berger M, et al. Oxidative base damage to DNA: recent mechanistic aspects, measurement and repair. In: Dizdaroglu M, Karakaya AE, eds. Advances in DNA Damage and Repair, Oxygen Radical Effects, Cellular Protection and Biological Consequences. Pp 47–58. New York: Plenum Press, 1999.
  • Ravanat JL, Douki T, Turesky R, et al. Measurement of oxidized bases in DNA. Comparison between HPLC-EC and GC-MS assays. J Chim Phys 1997; 94: 306–12.
  • Hong J, Oh CH, Johnson F, et al. Suppression of adventitious formation of 8-oxoguanine (TMS)4 form guanine during trimethylsilylation. Anal Biochem 1998; 261: 57–63.
  • Scott G. Atmospheric Oxidation and Antioxidants. Amsterdam: Elsevier, 1965.
  • Lunec J. ESCODD: European Standards Committee on Oxidative DNA Damage. Free Rad Res 1998; 29: 601–8.
  • Sentürker S, Karahalil B, Inal M, et al. Oxidative DNA base damage and antioxidant enzyme levels in childhood acute lymphoblastic leukemia. FEBS Lett 1997; 416: 286–90.
  • Rindgen D, Chaudharry AK, Blair IA. Applications of advanced mass spectrometry to detect oxidative DNA damage: carcinogenic implications. In: Aruoma OI, Halliwell B, eds. DNA and Free Radicals, Techniques, Mechanisms and Applications. St. Lucia: OICA International Press, 1998.
  • Aruoma OI, Halliwell B, Dizdaroglu M. Iron ion-dependent modification of bases in DNA by the superoxide radical-generating system hypoxanthine/xanthine oxidase. J Biol Chem 1989; 264: 13024–8.
  • Dizdaroglu M, Rao G, Halliwell B, et al. Damage to the DNA bases in mammalian chromatin by H2O2 in the presence of ferric and cupric ions. Arch Biochem Biophys 1991; 285: 317–24.
  • Farooq S, Bailey E, Farmer PB, et al. Determination of cis-thymine glycol in DNA by gas chromatography-mass spectrometry with selected ion recording and multiple reaction monitoring. J Chromatogr B 1997; 702: 49–60.
  • Gedik CM, Wood SG, Collins AR. Measuring oxidative damage to DNA: HPLC and the comet assay compared. Free Radic Res 1998; 25: 609–15.
  • Collins AR. The comet assay: a novel approach to measuring DNA oxidation. In: Aruoma OI, Halliwell B, eds. DNA and Free Radical, Techniques, Mechanisms and Applications. St. Lucia: OICA International Press, 1998.
  • Rehman A, Jenner A, Halliwell B. Gas chromatography-mass spectrometry analysis of DNA: optimization of procedures for the isolation and analysis of DNA from human blood. Methods Enzymol 2000; 319: 401–17.
  • Malins DC, Gunselman SJ. Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry reveal a remarkable degree of structural damage in the DNA of wild fish exposed to toxic chemicals. Proc Natl Acad Sci USA 1994; 91: 1308–41.
  • Abalea V, Cillard J, Dubois MP, et al. Iron-induced oxidative DNA damage and its repair in primary rat hepatocyte culture. Carcinogenesis 1998; 19: 1053–9.
  • Spencer JPE, Jenner A, Aruoma OI, et al. Oxidative DNA damage in human respiratory tract epithelial cells. Time course in relation to DNA strand breakage. Biochem Biophys Res Commun 1996; 224: 17–22.
  • Swarts GG, Smith GS, Miao L, Wheeler KT. Effect of formic acid hydrolysis on the quantitative analysis of radiation-induced DNA-base damage products assayed by gas chromatogra-phy-mass spectrometry. Rad Environ Biophysics 1996; 35: 41–53.
  • Halliwell B, Dizdaroglu M. The measurement of oxidative damage to DNA by HPLC and GC/ MS techniques. Free Radic Res Commun 1992; 16: 75–87.
  • Breimer LH. Repair of DNA damage induced by reactive oxygen species. Free Radic Res Commun 1991; 14: 159–71.
  • Maccubbin A, Evans M, Paul CR, et al. Enzymatic excission of radiation-induced lesions from DNA model compounds. Rad Res 1991; 126: 21–6.
  • Calltey RC, Glover SE. Evaluated 8-hydroxydeoxyguanosine in hepatic DNA of rats following exposure to peroxisome proliferations: relationship to carcinogenesis and nuclear localization. Carcinogenesis 1993; 14: 2495–9.
  • Hariharan PV, Cerutti P. Formation and repair of ?-ray induced thymine damage in Micrococ-cus radiodurans. J Mol Biol 1972; 66: 65–81.
  • Hariharan PV, Courtney J, Eleczko S. Production of hydroxyl radicals in cell systems exposed to haematoporphyrin and red light. Int J Rad Biol 1980; 37: 691–4.
  • Kasai H, Crain PF, Kuchino Y, et al. Formation of 8-hydroxyguanine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair. Carcinogenesis 1986; 7: 1849–51.
  • Poulsen HE, Loft S. Interpretation of oxidative DNA modification: relation between tissue levels, excretion of urinary repair products and single cell gel electrophoresis (comet assay). In: Aruoma OI, Halliwell B, eds. DNA and Free Radicals, Techniques, Mechanisms and Applications. St. Lucia: OICA International Press, 1998.
  • Kiyosawa H, Suko M, Okudaira H, et al. Cigarette smoking induces formation of 8-hydroxydeoxyguanosine, one of the oxidative DNA damages, in human peripheral leukocytes. Free Radic Res Commun 1990; 11: 23–7.
  • Deng XS, Tuo J, Poulsen HE, et al. Prevention of oxidative DNA damage in rats by Brussels sprouts. Free Radic Res 1998; 28: 323–33.
  • Pagana G, Rice-Evans CA. The identification of flavonoids as glycosides in human plasma. FEBS Lett 1997; 401: 78–82.
  • Whiteman M, Spencer JPE, Halliwell B. Hypochlorus acid-induced base modification in isolated calf thymus DNA. Chem Res Toxicol 1997; 10: 1240–6.
  • Whiteman M, Spencer JPE, Jenner A, et al. Hypochlorus acid-induced DNA base modifications: potentiation by nitrite. Biophys Res Commun 1999; 257: 572–6.
  • Fuciarelli AF, Wegher BJ, Blakely WF. Yields of radiation-induced base products in DNA, effects of DNA conforamation and gassing conditions. Int J Rad Biol 1990; 58: 397–415.
  • Alam ZI, Jenner A, Daniel SE, et al. Oxidative DNA damage in the Parkinsonion brain: an apparent slective increase in 8-hydroxyguanine levels in substantia negra. J Neurochem 1997; 69: 1196–203.
  • Sian J, Dexter DT, Lees AJ, et al. Alterations in glutathione levels in Parkinson’s desease and other neurodegenerative disorders affecting basal ganglia. Ann Neurol 1994; 36: 348–55.
  • Alam ZI, Daniel SE, Lees AJ, et al. A generalised increase in protein carbonyls in the brain in Parkinson’s but not incidental Lewy body disease. J Neurochem 1997; 69: 1326–9.
  • Halliwell B. Can oxidative DNA damage be used as a biomarker of cancer risk in humans? Problems, resolutions and preliminary results from nutritional supplementation studies. Free Radic Res 1998; 29: 469–86.
  • Cadet J, Berger M. Radiation-induced decomposition of the purine bases within DNA and related model compounds. Int J Rad Biol 1985; 47: 127–43.
  • Dizdaroglu M, Jaruga P, Rodriguez H. Identification and quentification of 8,5?-cyclo-2?-deoxyadenosine in DNA by liquid chromatography/mass spectrometry. Free Radic Biol Med 2001; 30: 774–84.
  • Jaruga P, Rodriguez H, Dizdaroglu M. Measurement of 8-hydroxy-2?-deoxyguanosine in DNA by liquid chromatography/mass spectrometry. Free Radic Biol Med 2001; 31: 336–44.
  • Tuo J, Muftuoglu M, Chen C, et al. The Cockayne Syndrome group B gene product is involved in general genome base excision repair of 8-hydroxguanine in DNA. J Biol Chem 2001; 276: 45772–9.
  • Dizdaroglu M. Formation of an 8-hydroxyguanine moiety in deoxyribonucleic acid on ?-irradation in aqueous solution. Biochemistry 1985; 24: 4476–81.
  • Karakaya A, Jaruga P, Bohr VA, et al. Kinetics of excision of purine lesions from DNA by Escherichia coli Fpg protein. Nucl Acid Res 1997; 25: 474–9.
  • Lenton KJ, Therriault H, Fulop T, et al. Glutathione and ascorbate are negativily correlated with oxidative DNA damage in human lymphocytes. Carcinogenesis 1999; 20: 607–13.
  • Davidson JN. The Biochemistry of Nucleic Acids. London: Chapman and Hall, 1972.
  • Frelon S, Douki T, Ravanat JL, et al. High-performance liquid chromatogrpahy-tandem mass spectrometry measurement of radiation-induced base damage. Chem Res Toxicol 2000; 13: 1002–10.
  • Weiman A, Belling D, Poulsen HE. Measurement of 8-oxo-2?-deoxyguaosine and 8-oxo-2?-oxyadenosine in DNA and human urine by high performance liquid chroma-tography-electrosprau tandem mass spectrometry. Free Radic Biol Med 2001; 30: 757–64.
  • Friedberg EC. Cockayne Syndrome, a primary defect in DNA repair, transcript, both or neither? Bioessays 1996; 18: 731–8.
  • Nance MA, Berry SA. Cockayne Syndrome: review of 140 cases. Am J Med Genet 1992; 42: 68–84.
  • Balajee AS, DeSantis LP, Brosh RMJ, et al. Role of the ATPase domain of the Cockayne Syndrome group B protein in UV-induced apoptosis. Oncogen 2000; 19: 477–89.
  • Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from nucleated cells. Nucl Acids Res 1988; 16: 1215.

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