1,272
Views
11
CrossRef citations to date
0
Altmetric
Reviews

Metataxonomic and metagenomic approaches for the study of undefined strain starters for cheese manufacture

ORCID Icon, ORCID Icon, ORCID Icon & ORCID Icon

References

  • Afshari, R., C. J. Pillidge, D. A. Dias, A. M. Osborn, and H. Gill. 2020. Cheesomics: The future pathway to understanding cheese flavour and quality. Critical Reviews in Food Science and Nutrition 60 (1):33–47. doi: 10.1080/10408398.2018.1512471.
  • Alessandria, V., I. Ferrocino, F. De Filippis, M. Fontana, K. Rantsiou, D. Ercolini, and L. Cocolin. 2016. Microbiota of an Italian Grana like cheese during manufacture and ripening unraveled by 16S rRNA-based approaches. Applied and Environmental Microbiology 82 (13):3988–95. doi: 10.1128/AEM.00999-16.
  • Alexeeva, S., J. A. Guerra Martínez, M. Spus, and E. J. Smid. 2018. Spontaneously induced prophages are abundant in a naturally evolved bacterial starter culture and deliver competitive advantage to the host. BMC Microbiology 18 (1):120. doi: 10.1186/s12866-018-1229-1.
  • Bertani, G., A. Levante, C. Lazzi, B. Bottari, M. Gatti, and E. Neviani. 2020. Dynamics of a natural bacterial community under technological and environmental pressures: The case of natural whey starter for Parmigiano Reggiano cheese. Food Research International (Ottawa, Ont.) 129:108860. doi: 10.1016/j.foodres.2019.108860.
  • Bissonnette, F., S. Labrie, H. Deveau, M. Lamoureux, and S. Moineau. 2000. Characterization of mesophilic mixed starter cultures used for the manufacture of aged Cheddar cheese. Journal of Dairy Science 83 (4):620–7. doi: 10.3168/jds.S0022-0302(00)74921-6.
  • Bottari, B., C. Agrimonti, M. Gatti, E. Neviani, and N. Marmiroli. 2013. Development of a multiplex real time PCR to detect thermophilic lactic acid bacteria in natural whey starters. International Journal of Food Microbiology 160 (3):290–7. doi: 10.1016/j.ijfoodmicro.2012.10.011.
  • Bourdichon, F., S. Casaregola, C. Farrokh, J. C. Frisvad, M. L. Gerds, W. P. Hammes, J. Harnett, G. Huys, S. Laulund, A. Ouwehand, et al. 2012. Food fermentations: Microorganisms with technological beneficial use. International Journal of Food Microbiology 154 (3):87–97. doi: 10.1016/j.ijfoodmicro.2011.12.030.
  • Calasso, M., D. Ercolini, L. Mancini, G. Stellato, F. Minervini, R. D. Cagno, M. De Angelis, and M. Gobbetti. 2016. Relationships among house, rind and core microbiotas during manufacture of traditional Italian cheeses at the same dairy plant. Food Microbiology 54:115–26. doi: 10.1016/j.fm.2015.10.008.
  • Callahan, B., P. McMurdie, and S. Holmes. 2017. Exact sequence variants should replace operational taxonomic units in marker-gene data analysis. The ISME Journal 11 (12):2639–43. doi: 10.1038/ismej.2017.119.
  • Carminati, D., M. Zago, and G. Giraffa. 2012. Ecological aspects of phage contamination in natural whey and milk starters. In Bacteriophages in dairy processing, eds. A. L. Quiberoni and J. A. Reinheimer, 79–97. New York, NY: Nova Science Publishers, Inc.
  • De Filippis, F., A. Genovese, P. Ferranti, J. A. Gilbert, and D. Ercolini. 2016. Metatranscriptomics reveals temperature-driven functional changes in microbiome impacting cheese maturation rate. Scientific Reports 6:21871. doi: 10.1038/srep21871.
  • De Filippis, F., A. La Storia, G. Stellato, M. Gatti, and D. Ercolini. 2014. A selected core microbiome drives the early stages of three popular Italian cheese manufactures. PLoS One 9 (2):e89680. doi: 10.1371/journal.pone.0089680.
  • De Filippis, F., E. Parente, and D. Ercolini. 2018. Recent past, present, and future of the food microbiome. Annual Review of Food Science and Technology 9 (1):589–608. doi: 10.1146/annurev-food-030117-012312.
  • Delgado, S., C. T. C. C. Rachid, E. Fernández, T. Rychlik, A. Alegría, R. S. Peixoto, and B. Mayo. 2013. Diversity of thermophilic bacteria in raw, pasteurized and selectively-cultured milk, as assessed by culturing, PCR-DGGE and pyrosequencing. Food Microbiology 36 (1):103–11. doi: 10.1016/j.fm.2013.04.015.
  • Dobson, A., O. O'sullivan, P. Cotter, P. Ross, and C. Hill. 2011. High-throughput sequence-based analysis of the bacterial composition of kefir and an associated kefir grain. FEMS Microbiology Letters 320 (1):56–62. doi: 10.1111/j.1574-6968.2011.02290.x.
  • Emerson, J. B., R. I. Adams, C. M. Betancourt Román, B. Brooks, D. A. Coil, K. E. Dahlhausen, H. H. Ganz, E. M. Hartmann, T. Hsu, N. B. Justice, et al. 2017. Schrödinger’s microbes: Tools for distinguishing the living from the dead in microbial ecosystems. Microbiome 5 (1):86. doi: 10.1186/s40168-017-0285-3.
  • Ercolini, D., F. De Filippis, A. L. Storia, and M. Iacono. 2012. “Remake” by high-throughput sequencing of the microbiota involved in the production of water buffalo mozzarella cheese. Applied and Environmental Microbiology 78 (22):8142–5. doi: 10.1128/aem.02218-12.
  • Erkus, O., V. C. L. de Jager, R. T. C. M. Geene, I. van Alen-Boerrigter, L. Hazelwood, S. A. F. T. van Hijum, M. Kleerebezem, and E. J. Smid. 2016. Use of propidium monoazide for selective profiling of viable microbial cells during Gouda Cheese ripening. International Journal of Food Microbiology 228:1–9. doi: 10.1016/j.ijfoodmicro.2016.03.027.
  • Erkus, O., V. C. L. de Jager, M. Spus, I. J. van Alen-Boerrigter, I. M. H. van Rijswijck, L. Hazelwood, P. W. M. Janssen, S. A. F. T. van Hijum, M. Kleerebezem, and E. J. Smid. 2013. Multifactorial diversity sustains microbial community stability. The ISME Journal 7 (11):2126–36. doi: 10.1038/ismej.2013.108.
  • Faust, K. 2019. Towards a better understanding of microbial community dynamics through high-throughput cultivation and data integration. mSystems 4 (3):e0010119. doi: 10.1128/mSystems.00101-19.
  • Fornasari, M., L. Rossetti, D. Carminati, and G. Giraffa. 2006. Cultivability of Streptococcus thermophilus in Grana Padano cheese whey starters. FEMS Microbiology Letters 257 (1):139–44. doi: 10.1111/j.1574-6968.2006.00155.x.
  • Frantzen, C., and H. Holo. 2019. Unprecedented diversity of Lactococcal Group 936 bacteriophages revealed by amplicon sequencing of the portal protein gene. Viruses 11 (5):443. doi: 10.3390/v11050443.
  • Frantzen, C. A., H. P. Kleppen, and H. Holo. 2017. Lactococcus lactis diversity in undefined mixed dairy starter cultures as revealed by comparative genome analyses and targeted amplicon sequencing of EpsD. Applied and Environmental Microbiology 84 (3):2725. doi: 10.1128/AEM.02199-17.
  • Frantzen, C. A., W. Kot, T. B. Pedersen, Y. M. Ardö, J. R. Broadbent, H. Neve, L. H. Hansen, F. Dal Bello, H. M. Ostlie, H. P. Kleppen, et al. 2017. Genomic characterization of dairy associated Leuconostoc species and diversity of Leuconostocs in undefined mixed mesophilic starter cultures. Frontiers in Microbiology 8:132–14. doi: 10.3389/fmicb.2017.00132.
  • Gatti, M., B. Bottari, C. Lazzi, E. Neviani, and G. Mucchetti. 2014. Invited review: Microbial evolution in raw-milk, long-ripened cheeses produced using undefined natural whey starters. Journal of Dairy Science 97 (2):573–91. doi: 10.3168/jds.2013-7187.
  • Gatti, M., C. Trivisano, E. Fabrizi, E. Neviani, and F. Gardini. 2004. Biodiversity among Lactobacillus helveticus strains isolated from different natural whey starter cultures as revealed by classification trees. Applied and Environmental Microbiology 70 (1):182–90. doi: 10.1128/aem.70.1.182-190.2004.
  • Giello, M., A. La Storia, F. Masucci, A. Di Francia, D. Ercolini, and F. Villani. 2017. Dynamics of bacterial communities during manufacture and ripening of traditional caciocavallo of Castelfranco cheese in relation to cows' feeding. Food Microbiology 63:170–7. doi: 10.1016/j.fm.2016.11.016.
  • Guidone, A., A. Ricciardi, A. Romaniello, M. G. Bonomo, G. Morone, T. Zotta, and E. Parente. 2016. Microbial changes of natural milk cultures for Mozzarella cheese during repeated propagation cycles. LWT - Food Science and Technology 65 (C):572–9. doi: 10.1016/j.lwt.2015.08.031.
  • Jin, H., L. Mo, L. Pan, Q. Hou, C. Li, I. Darima, and J. Yu. 2018. Using PacBio sequencing to investigate the bacterial microbiota of traditional Buryatian cottage cheese and comparison with Italian and Kazakhstan artisanal cheeses. Journal of Dairy Science 101 (8):6885–96. doi: 10.3168/jds.2018-14403.
  • Johansen, E., G. Øregaard, K. Sørensen, and P. Derkx. 2015. Modern approaches for isolation, selection, and improvement of bacterial strains for fermentation applications. In Advances in fermented foods and beverages; Part Two: Fermentation microbiology, ed. W. Holzapfel, 227–48. Oxford, UK: Woodhead Publishing. doi: 10.1016/b978-1-78242-015-6.00010-4.
  • Josephsen, J., A. Petersen, H. Neve, and E. Nielsen. 1999. Development of lytic Lactococcus lactis bacteriophages in a cheddar cheese plant. International Journal of Food Microbiology 50 (3):163–71. doi: 10.1016/S0168-1605(99)00098-7.
  • Kamilari, E., M. Tomazou, A. Antoniades, and D. Tsaltas. 2019. High throughput sequencing technologies as a new toolbox for deep analysis, characterization and potentially authentication of protection designation of origin cheeses? International Journal of Food Science 2019:5837301. doi: 10.1155/2019/5837301.
  • Kamimura, B. A., L. Cabral, M. F. Noronha, R. C. Baptista, H. M. Nascimento, and A. S. Sant'Ana. 2020. Amplicon sequencing reveals the bacterial diversity in milk, dairy premises and Serra Da Canastra artisanal cheeses produced by three different farms. Food Microbiology 89:103453. doi: 10.1016/j.fm.2020.103453.
  • Kamimura, B. A., F. De Filippis, A. S. Sant'Ana, and D. Ercolini. 2019. Large-scale mapping of microbial diversity in artisanal brazilian cheeses. Food Microbiology 80:40–9. doi: 10.1016/j.fm.2018.12.014.
  • Kask, S., K. Adamberg, A. Orłowski, F. Vogensen, P. Møller, Y. Ardö, and T. Paalme. 2003. Physiological properties of Lactobacillus paracasei, L. danicus and L. curvatus strains isolated from Estonian semi-hard cheese. Food Research International 36 (9–10):1037–46. doi: 10.1016/j.foodres.2003.08.002.
  • Kelleher, P., J. Murphy, J. Mahony, and D. van Sinderen. 2015. Next-Generation Sequencing as an approach to dairy starter selection. Dairy Science & Technology 95 (5):545–68. doi: 10.1007/s13594-015-0227-4.
  • Kleppen, H., T. Bang, I. Nes, and H. Holo. 2011. Bacteriophages in milk fermentations: Diversity fluctuations of normal and failed fermentations. International Dairy Journal 21 (9):592–600. doi: 10.1016/j.idairyj.2011.02.010.
  • Kumar, S. S., and A. R. Ghosh. 2019. Assessment of bacterial viability: A comprehensive review on recent advances and challenges. Microbiology (Reading, England) 165 (6):593–610. doi: 10.1099/mic.0.000786.
  • Lagier, J.-C., P. Hugon, S. Khelaifia, P.-E. Fournier, B. L. Scola, and D. Raoult. 2015. The rebirth of culture in microbiology through the example of culturomics to study human gut microbiota. Clinical Microbiology Reviews 28 (1):237–64. doi: 10.1128/cmr.00014-14.
  • Li, J., Y. Zheng, H. Xu, X. Xi, Q. Hou, S. Feng, L. Wuri, Y. Bian, Z. Yu, L. Kwok, et al. 2017. Bacterial microbiota of Kazakhstan cheese revealed by single molecule real time (SMRT) sequencing and its comparison with Belgian, Kalmykian and Italian artisanal cheeses. BMC Microbiology 17 (1):13. doi: 10.1186/s12866-016-0911-4.
  • Limsowtin, G. K. Y., I. B. Powell, and E. Parente. 1995. Types of starters. In Dairy starter cultures, eds. T. Cogan and J. P. Accolas, 101–29. New York, NY: VCH Publishers.
  • Mahony, J., A. Moscarelli, P. Kelleher, G. Lugli, M. Ventura, L. Settanni, and D. van Sinderen. 2017. Phage biodiversity in artisanal cheese wheys reflects the complexity of the fermentation process. Viruses 9 (3):45. doi: 10.3390/v9030045.
  • Marino, M., G. Wittenau, E. Saccà, F. Cattonaro, A. Spadotto, N. Innocente, S. Radovic, E. Piasentier, and F. Marroni. 2019. Metagenomic profiles of different types of Italian high-moisture Mozzarella cheese. Food Microbiology 79:123–31. doi: 10.1016/j.fm.2018.12.007.
  • Masoud, W., M. Takamiya, F. Vogensen, S. Lillevang, W. Al-Soud, S. Sørensen, and M. Jakobsen. 2011. Characterization of bacterial populations in Danish raw milk cheeses made with different starter cultures by denaturating gradient gel electrophoresis and pyrosequencing. International Dairy Journal 21 (3):142–8. doi: 10.1016/j.idairyj.2010.10.007.
  • Mauriello, G., L. Moio, A. Genovese, and D. Ercolini. 2003. Relationships between flavoring capabilities, bacterial composition, and geographical origin of natural whey cultures used for traditional Water-Buffalo Mozzarella Cheese manufacture. Journal of Dairy Science 86 (2):486–97. doi: 10.3168/jds.S0022-0302(03)73627-3.
  • Meola, M., E. Rifa, N. Shani, C. Delbès, H. Berthoud, and C. Chassard. 2019. DAIRYdb: A manually curated reference database for improved taxonomy annotation of 16S rRNA gene sequences from dairy products. BMC Genomics 20 (1):560. doi: 10.1186/s12864-019-5914-8.
  • Montel, M., S. Buchin, A. Mallet, C. Delbès-Paus, D. Vuitton, N. Desmasures, and F. Berthier. 2014. Traditional cheeses: Rich and diverse microbiota with associated benefits. International Journal of Food Microbiology 177 (C):136–54. doi: 10.1016/j.ijfoodmicro.2014.02.019.
  • Morandi, S., G. Battelli, T. Silvetti, A. Goss, N. Cologna, and M. Brasca. 2019. How the biodiversity loss in natural whey culture is affecting ripened cheese quality? The case of Trentingrana Cheese. LWT - Food Science and Technology 115:108480. doi: 10.1016/j.lwt.2019.108480.
  • Moser, A., K. Schafroth, L. Meile, L. Egger, R. Badertscher, and S. Irmler. 2018. Population dynamics of Lactobacillus helveticus in Swiss Gruyère-type cheese manufactured with natural whey cultures. Frontiers in Microbiology 9:637. doi: 10.3389/fmicb.2018.00637.
  • Muhammed, M. K., W. Kot, H. Neve, J. Mahony, J. L. Castro-Mejía, L. Krych, L. H. Hansen, D. S. Nielsen, S. J. Sørensen, K. J. Heller, et al. 2017. Metagenomic analysis of dairy bacteriophages: Extraction method and pilot study on whey samples derived from using undefined and defined mesophilic starter cultures. Applied and Environmental Microbiology 83 (19):e00888-17. doi: 10.1128/AEM.00888-17.
  • Nielsen, W. 1998. Long term use of a Cheddar starter and development of phages with homology to its bacteria. International Dairy Journal 8 (12):1003–9.
  • Oksanen, J., F. G. Blanchet, M. Friendly, R. Kindt, P. Legendre, D. McGlinn, P. R. Minchin, R. B. O'Hara, G. L. Simpson, and P. Solymos. 2019. Vegan: Community ecology package. R package version 2.5-6. https://CRAN.R-project.org/package=vegan.
  • Parente, E. 2006. Diversity and dynamics of microbial communities in natural and mixed starter cultures. Australian Journal of Dairy Technology 61 (2):1–8.
  • Parente, E., T. M. Cogan, and I. B. Powell. 2017. Starter cultures: General aspects. In Cheese chemistry, physics and microbiology, eds. P. L. H. McSweeney, P. F. Fox, P. D. Cotter and D. W. Everett, vol. 1, 201–26. Cambridge, MA: Academic Press. doi: 10.1016/b978-0-12-417012-4.00008-9.
  • Parente, E., F. De Filippis, D. Ercolini, A. Ricciardi, and T. Zotta. 2019. Advancing integration of data on food microbiome studies: FoodMicrobionet 3.1, a major upgrade of the FoodMicrobionet Database. International Journal of Food Microbiology 305:108249. doi: 10.1016/j.ijfoodmicro.2019.108249.
  • Parente, E., A. Guidone, A. Matera, F. De Filippis, G. Mauriello, and A. Ricciardi. 2016. Microbial community dynamics in thermophilic undefined milk starter cultures. International Journal of Food Microbiology 217 (C):59–67. doi: 10.1016/j.ijfoodmicro.2015.10.014.
  • Parente, E., A. Ricciardi, and T. Zotta. 2020. The microbiota of dairy milk: A review. International Dairy Journal 107:104714. doi: 10.1016/j.idairyj.2020.104714.
  • Parente, E., M. A. Rota, A. Ricciardi, and F. Clementi. 1997. Characterization of natural starter cultures used in the manufacture of Pasta Filata cheese in Basilicata (Southern Italy). International Dairy Journal 7 (12):775–83. doi: 10.1016/S0958-6946(97)00093-9.
  • Pollock, J., L. Glendinning, T. Wisedchanwet, and M. Watson. 2018. The madness of microbiome: Attempting to find consensus “Best Practice” for 16S Microbiome Studies. Applied and Environmental Microbiology 84 (7):3225. doi: 10.1128/AEM.02627-17.
  • Powell, I. 2010. Issues in cheese starter culture microbiology. Australian Journal of Dairy Technology 65 (2):40–4.
  • Pujato, S., A. Quiberoni, and D. Mercanti. 2019. Bacteriophages on dairy foods. Journal of Applied Microbiology 126 (1):14–30. doi: 10.1111/jam.14062.
  • Reinheimer, J. A., A. Quiberoni, P. Tailliez, A. G. Binetti, and V. B. Suarez. 1996. The lactic acid microflora of natural whey starters used in Argentina for hard cheese production. International Dairy Journal 6 (8–9):869–79. doi: 10.1016/0958-6946(96)00014-3.
  • Ricciardi, A., F. Filippis, T. Zotta, A. Facchiano, D. Ercolini, and E. Parente. 2016. Polymorphism of the phosphoserine phosphatase gene in Streptococcus thermophilus and its potential use for typing and monitoring of population diversity. International Journal of Food Microbiology 236:138–47. doi: 10.1016/j.ijfoodmicro.2016.07.031.
  • Saltaji, S., O. Rué, V. Sopena, S. Sablé, F. Tambadou, S. Didelot, and R. Chevrot. 2020. Lactococcus lactis diversity revealed by targeted amplicon sequencing of PurR gene, metabolic comparisons and antimicrobial properties in an undefined mixed starter culture used for soft-cheese manufacture. Foods 9 (5):622. doi: 10.3390/foods9050622.
  • Sant’Anna, F. M., S. U. Wetzels, S. H. S. Cicco, R. C. Figueiredo, G. A. Sales, N. C. Figueiredo, C. A. Nunes, S. Schmitz-Esser, E. Mann, M. Wagner, et al. 2019. Microbial shifts in Minas artisanal cheeses from the Serra Do Salitre Region of Minas Gerais, Brazil throughout ripening Time. Food Microbiology 82:349–62. doi: 10.1016/j.fm.2019.02.016.
  • Skeie, S., M. Håland, I. Thorsen, J. Narvhus, and D. Porcellato. 2019. Bulk tank raw milk microbiota differs within and between farms: A moving goalpost challenging quality control. Journal of Dairy Science 102 (3):1959–71. doi: 10.3168/jds.2017-14083.
  • Smid, E. J., O. Erkus, M. Spus, J. C. M. Wolkers-Rooijackers, S. Alexeeva, and M. Kleerebezem. 2014. Functional implications of the microbial community structure of undefined mesophilic starter cultures. Microbial Cell Factories 13 (Suppl 1):S2. doi: 10.1186/1475-2859-13-S1-S2.
  • Sohier, D., S. Pavan, A. Riou, J. Combrisson, and F. Postollec. 2014. Evolution of microbiological analytical methods for dairy industry needs. Frontiers in Microbiology 5:16. doi: 10.3389/fmicb.2014.00016.
  • Somerville, V., S. Lutz, M. Schmid, D. Frei, A. Moser, S. Irmler, J. E. Frey, and H. C. Ahrens. 2019. Long-read based de novo assembly of low-complexity metagenome samples results in finished genomes and reveals insights into strain diversity and an active phage system. BMC Microbiology 19 (1):143. doi: 10.1186/s12866-019-1500-0.
  • Spus, M., M. Li, S. Alexeeva, J. C. M. Wolkers-Rooijackers, M. H. Zwietering, T. Abee, and E. J. Smid. 2015. Strain diversity and phage resistance in complex dairy starter cultures. Journal of Dairy Science 98 (8):5173–82. doi: 10.3168/jds.2015-9535.
  • Van Rossum, T., P. Ferretti, O. Maistrenko, and P. Bork. 2020. Diversity within species: Interpreting strains in microbiomes. Nature Reviews. Microbiology 18 (9):491–506. doi: 10.1038/s41579-020-0368-1.
  • Yang, C., F. Zhao, Q. Hou, J. Wang, M. Li, and Z. Sun. 2020. PacBio sequencing reveals bacterial community diversity in cheeses collected from different regions. Journal of Dairy Science 103 (2):1238–49. doi: 10.3168/jds.2019-17496.
  • Zago, M., B. Bonvini, L. Rossetti, A. Meucci, G. Giraffa, and D. Carminati. 2015. Biodiversity of Lactobacillus helveticus bacteriophages isolated from cheese whey starters. The Journal of Dairy Research 82 (2):242–7. doi: 10.1017/s0022029915000151.
  • Zago, M., L. Orrù, L. Rossetti, A. Lamontanara, M. E. Fornasari, B. Bonvini, A. Meucci, D. Carminati, L. Cattivelli, and G. Giraffa. 2017. Survey on the phage resistance mechanisms displayed by a dairy Lactobacillus helveticus strain. Food Microbiology 66:110–6. doi: 10.1016/j.fm.2017.04.014.
  • Zheng, J., S. Wittouck, E. Salvetti, C. M. A. P. Franz, H. M. B. Harris, P. Mattarelli, P. W. O'Toole, B. Pot, P. Vandamme, J. Walter, et al. 2020. A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae. International Journal of Systematic and Evolutionary Microbiology 70 (4):2782–858. doi: 10.1099/ijsem.0.004107.
  • Zinno, P., T. Janzen, M. Bennedsen, D. Ercolini, and G. Mauriello. 2010. Characterization of Streptococcus thermophilus lytic bacteriophages from mozzarella cheese plants. International Journal of Food Microbiology 138 (1–2):137–44. doi: 10.1016/j.ijfoodmicro.2009.12.008.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.