Recent advances in the development of bitter gourd seed oil: from chemical composition to potential applications

References

• Ahmad, Z., K. F. Zamhuri, A. Yaacob, C. H. Siong, M. Selvarajah, A. Ismail, and M. N. Hakim. 2012. In vitro anti-diabetic activities and chemical analysis of polypeptide-k and oil isolated from seeds of Momordica charantia (bitter gourd). Molecules (Basel, Switzerland) 17 (8):9631–40. doi: 10.3390/molecules17089631.
   PubMed Web of Science ®Google Scholar
• Ali, M. A., M. A. Sayeed, M. S. Reza, M. S. Yeasmin, and A. M. Khan. 2008. Characteristics of seed oils and nutritional compositions of seeds from different varieties of Momordica charantia Linn. cultivated in Bangladesh. Czech Journal of Food Sciences 26 (4):275–83. doi: 10.17221/1123-CJFS.
   Web of Science ®Google Scholar
• Anjum, F., M. Shahid, S. A. Bukhari, S. Anwar, and S. Latif. 2012. Study of quality characteristics and efficacy of extraction solvent/technique on the antioxidant activity of bitter gourd seed. Journal of Food Processing & Technology 04 (02):205. doi: 10.4172/2157-7110.1000205.
   Google Scholar
• Białek, A., M. Teryks, and A. Tokarz. 2014. Conjugated linolenic acids (CLnA, super CLA)-natural sources and biological activity. Postepy Higieny i Medycyny Doswiadczalnej (Online) 68:1238–50. doi: 10.5604/17322693.1127881.
   PubMedGoogle Scholar
• Bouarab Chibane, L., P. Degraeve, H. Ferhout, J. Bouajila, and N. Oulahal. 2019. Plant antimicrobial polyphenols as potential natural food preservatives. Journal of the Science of Food and Agriculture 99 (4):1457–74. doi: 10.1002/jsfa.9357.
   PubMed Web of Science ®Google Scholar
• Braca, A., T. Siciliano, M. D’Arrigo, and M. P. Germanò. 2008. Chemical composition and antimicrobial activity of Momordica charantia seed essential oil. Fitoterapia 79 (2):123–5. doi: 10.1016/j.fitote.2007.11.002.
   PubMed Web of Science ®Google Scholar
• Cao, X., and Y. Ito. 2003. Supercritical fluid extraction of grape seed oil and subsequent separation of free fatty acids by high-speed counter-current chromatography. Journal of Chromatography. A 1021 (1–2):117–24. doi: 10.1016/j.chroma.2003.09.001.
   PubMed Web of Science ®Google Scholar
• Chan, F. K., C. Hsu, T. C. Li, W. H. Chen, K. T. Tseng, and P. M. Chao. 2018. Bitter melon seed oil increases mitochondrial content in gastrocnemius muscle and improves running endurance in sedentary C57BL/6J mice. The Journal of Nutritional Biochemistry 58:150–7. doi: 10.1016/j.jnutbio.2018.05.008.
   PubMed Web of Science ®Google Scholar
• Chang, Y. Y., H. M. Su, S. H. Chen, W. T. Hsieh, J. H. Chyuan, and P. M. Chao. 2016. Roles of peroxisome proliferator-activated receptor alpha in bitter melon seed oil-corrected lipid disorders and conversion of alpha-eleostearic acid into rumenic acid in C57BL/6J mice. Nutrients 8 (12):805. doi: 10.3390/nu8120805.
   PubMed Web of Science ®Google Scholar
• Chen, G. C., W. H. Chen, K. T. Tseng, and P. M. Chao. 2017. The anti-adiposity effect of bitter melon seed oil is solely attributed to its fatty acid components. Lipids in Health and Disease 16 (1):186. doi: 10.1186/s12944-017-0578-3.
   PubMedGoogle Scholar
• Chen, P. H., G. C. Chen, M. F. Yang, C. H. Hsieh, S. H. Chuang, H. L. Yang, Y. H. Kuo, J. H. Chyuan, and P. M. Chao. 2012. Bitter melon seed oil-attenuated body fat accumulation in diet-induced obese mice is associated with cAMP-dependent protein kinase activation and cell death in white adipose tissue. The Journal of Nutrition 142 (7):1197–204. doi: 10.3945/jn.112.159939.
   PubMed Web of Science ®Google Scholar
• Chen, T. C., and Y. H. Ju. 2001. Polyunsaturated fatty acid concentrates from borage and linseed oil fatty acids. Journal of the American Oil Chemists’ Society 78 (5):485–8. doi: 10.1007/s11746-001-0290-3.
   Web of Science ®Google Scholar
• Chen, G. C., H. M. Su, Y. S. Lin, P. Y. Tsou, J. H. Chyuan, and P. M. Chao. 2016. A conjugated fatty acid present at high levels in bitter melon seed favorably affects lipid metabolism in hepatocytes by increasing NAD(+)/NADH ratio and activating PPARα, AMPK and SIRT1 signaling pathway. The Journal of Nutritional Biochemistry 33:28–35. doi: 10.1016/j.jnutbio.2016.03.009.
   PubMed Web of Science ®Google Scholar
• de Sousa, R. S., A. O. de Moraes Nogueira, V. G. Marques, R. M. Clementin, and V. R. de Lima. 2013. Effects of α-eleostearic acid on asolectin liposomes dynamics: Relevance to its antioxidant activity. Bioorganic Chemistry 51:8–15. doi: 10.1016/j.bioorg.2013.08.004.
   PubMed Web of Science ®Google Scholar
• Dhar Dubey, K. K., G. Sharma, and A. Kumar. 2019. Conjugated linolenic acids: Implication in cancer. Journal of Agricultural and Food Chemistry 67 (22):6091–101. doi: 10.1021/acs.jafc.9b01379.
   PubMed Web of Science ®Google Scholar
• Dhar, P., K. Chattopadhyay, D. Bhattacharyya, A. Roychoudhury, A. Biswas, and S. Ghosh. 2006. Antioxidative effect of conjugated linolenic acid in diabetic and non-diabetic blood: An in vitro study. Journal of Oleo Science 56 (1):19–24. doi: 10.5650/jos.56.19.
   PubMedGoogle Scholar
• Di Pasqua, R., G. Mamone, P. Ferranti, D. Ercolini, and G. Mauriello. 2010. Changes in the proteome of Salmonella enterica serovar Thompson as stress adaptation to sublethal concentrations of thymol. Proteomics 10 (5):1040–9. doi: 10.1002/pmic.200900568.
   PubMed Web of Science ®Google Scholar
• Domeneghini, C., A. Di Giancamillo, and C. Corino. 2006. Conjugated linoleic acids (CLAs) and white adipose tissue: How both in vitro and in vivo studies tell the story of a relationship. Histology and Histopathology 21 (6):663–72.
   PubMed Web of Science ®Google Scholar
• Durgam, V. R., and G. Fernandes. 1997. The growth inhibitory effect of conjugated linoleic acid on MCF-7 cells is related to the estrogen response system. Cancer Letters 116 (2):121–30. doi: 10.1016/S0304-3835(97)00192-4.
   PubMed Web of Science ®Google Scholar
• Fajardo, A. R., L. E. Cerdán, A. R. Medina, M. M. M. Martínez, E. H. Peña, and E. M. Grima. 2006. Concentration of eicosapentaenoic acid by selective esterification using lipases. Journal of the American Oil Chemists’ Society 83 (3):215–21. doi: 10.1007/s11746-006-1196-9.
   Google Scholar
• Fan, Z., and W. Zong. 2008. Study on technology of extracting balsam pear seed oil by supercritical fluid extraction. Cereal and Food Industry 15 (4):14–16. doi: 10.3969/j.issn.16725026.2008.04.005.
   Google Scholar
• Fang, E. F., and T. B. Ng. 2011. Bitter gourd (Momordica charantia) is a cornucopia of health: A review of its credited antidiabetic, anti-HIV, and antitumor properties. Current Molecular Medicine 11 (5):417–36. doi: 10.2174/156652411795976583.
   PubMed Web of Science ®Google Scholar
• Fei, P., M. A. Ali, S. Gong, Q. Sun, X. Bi, S. Liu, and L. Guo. 2018. Antimicrobial activity and mechanism of action of olive oil polyphenols extract against Cronobacter sakazakii. Food Control. 94:289–94. doi: 10.1016/j.foodcont.2018.07.022.
   Web of Science ®Google Scholar
• Fu, W. C., X. H. Gu, G. J. Tao, J. Tang, and Z. I. Jiang. 2009. Structure identification of triacylglycerols in the seed oil of Momordica charantia L. var. abbreviata Ser. Journal of the American Oil Chemists’ Society 86 (1):33–9. doi: 10.1007/s11746-008-1313-z.
   Web of Science ®Google Scholar
• Ginter, E., and V. Simko. 2013. Global prevalence and future of diabetes mellitus. In Diabetes. Advances in Experimental Medicine and Biology, ed. S. I. Ahmad, vol. 771, 35–41. New York: Springer.
   Google Scholar
• Gölükçü, M., R. Toker, F. Ayas, and N. ÇInar. 2014. Some physical and chemical properties of bitter melon (Momordica charantia L.) seed and fatty acid composition of seed oil. Derim 31 (1):17–24. doi: 10.16882/derim.2014.64310.
   Google Scholar
• Grossmann, M. E., N. K. Mizuno, M. L. Dammen, T. Schuster, A. Ray, and M. P. Cleary. 2009. Eleostearic acid inhibits breast cancer proliferation by means of an oxidation-dependent mechanism. Cancer Prevention Research (Philadelphia, Pa.) 2 (10):879–86. doi: 10.1158/1940-6207.CAPR-09-0088.
   PubMed Web of Science ®Google Scholar
• Gürdal, B., and S. Kültür. 2013. An ethnobotanical study of medicinal plants in Marmaris (Muğla, Turkey). Journal of Ethnopharmacology 146 (1):113–26. doi: 10.1016/j.jep.2012.12.012.
   PubMed Web of Science ®Google Scholar
• Hammann, S., U. Tillmann, M. Schröder, and W. Vetter. 2013. Profiling the fatty acids from a strain of the microalgae Alexandrium tamarense by means of high-speed counter-current chromatography and gas chromatography coupled with mass spectrometry. Journal of Chromatography A 1312:93–103. doi: 10.1016/j.chroma.2013.08.090.
   PubMed Web of Science ®Google Scholar
• Hayes, D. G. 2006. Effect of temperature programming on the performance of urea inclusion compound-based free fatty acid fractionation. Journal of the American Oil Chemists’ Society 83 (3):253–9. doi: 10.1007/s11746-006-1201-3.
   Google Scholar
• Horax, R., N. Hettiarachchy, A. Kannan, and P. Chen. 2010. Proximate composition and amino acid and mineral contents of Mormordica charantia L. pericarp and seeds at different maturity stages. Food Chemistry 122 (4):1111–5. doi: 10.1016/j.foodchem.2010.03.093.
   Web of Science ®Google Scholar
• Igarashi, M., and T. Miyazawa. 2000. Newly recognized cytotoxic effect of conjugated trienoic fatty acids on cultured human tumor cells. Cancer Letters 148 (2):173–9. doi: 10.1016/S0304-3835(99)00332-8.
   PubMed Web of Science ®Google Scholar
• İlhan, M., I. E. Bolat, İ. Süntar, K. H. Kutluay, D. A. U. Çankal, H. Keleş, and A. E. Küpeli. 2015. Topical application of olive oil macerate of Momordica charantia L. promotes healing of excisional and incisional wounds in rat buccal mucosa. Archives of Oral Biology 60 (12):1708–13. doi: 10.1016/j.archoralbio.2015.09.006.
   PubMed Web of Science ®Google Scholar
• Ip, C., S. F. Chin, J. A. Scimeca, and M. W. Pariza. 1991. Mammary cancer prevention by conjugated dienoic derivative of linoleic acid. Cancer Research 51 (22):6118–24.
   PubMed Web of Science ®Google Scholar
• Jing, Y., Z. Xiaohong, Z. Zuquan, C. Fang, Z. Jinshun, and L. Duo. 2015. Cytotoxic effects of punicic acid and α-eleostearic acid on human malignant melanoma A375 cells. Acta Scientific Nutritional Health 37:406–8.
   Google Scholar
• Joseph, B., and D. Jini. 2013. Antidiabetic effects of Momordica charantia (bitter melon) and its medicinal potency. Asian Pacific Journal of Tropical Disease 3 (2):93–102. doi: 10.1016/S2222-1808(13)60052-3.
   Google Scholar
• Kadam, M., and D. N. Bhowmick. 2006. Lipase-catalyzed modification of black currant oil. Journal of Food Lipids 13 (2):167–76. doi: 10.1111/j.1745-4522.2006.00042.x.
   Google Scholar
• Kennedy, A., K. Martinez, S. Schmidt, S. Mandrup, K. LaPoint, and M. McIntosh. 2010. Antiobesity mechanisms of action of conjugated linoleic acid. The Journal of Nutritional Biochemistry 21 (3):171–9. doi: 10.1016/j.jnutbio.2009.08.003.
   PubMed Web of Science ®Google Scholar
• Kobori, M., M. Ohnishi-Kameyama, Y. Akimoto, C. Yukizaki, and M. Yoshida. 2008. Alpha-eleostearic acid and its dihydroxy derivative are major apoptosis-inducing components of bitter gourd. Journal of Agricultural and Food Chemistry 56 (22):10515–20. doi: 10.1021/jf8020877.
   PubMed Web of Science ®Google Scholar
• Kohno, H., R. Suzuki, R. Noguchi, M. Hosokawa, K. Miyashita, and T. Tanaka. 2002. Dietary conjugated linolenic acid inhibits azoxymethane-induced colonic aberrant crypt foci in rats. Japanese Journal of Cancer Research 93 (2):133–42. doi: 10.1111/j.1349-7006.2002.tb01251.x.
   PubMedGoogle Scholar
• Kohno, H., Y. Yasui, R. Suzuki, M. Hosokawa, K. Miyashita, and T. Tanaka. 2004. Dietary seed oil rich in conjugated linolenic acid from bitter melon inhibits azoxymethane-induced rat colon carcinogenesis through elevation of colonic PPARgamma expression and alteration of lipid composition. International Journal of Cancer 110 (6):896–901. doi: 10.1002/ijc.20179.
   PubMed Web of Science ®Google Scholar
• Kwatra, D., P. Dandawate, S. Padhye, and S. Anant. 2016. Bitter melon as a therapy for diabetes, inflammation, and cancer: A panacea? Current Pharmacology Reports 2 (1):34–44. doi: 10.1007/s40495-016-0045-2.
   Google Scholar
• Leung, L., R. Birtwhistle, J. Kotecha, S. Hannah, and S. Cuthbertson. 2009. Anti-diabetic and hypoglycaemic effects of Momordica charantia (bitter melon): A mini review. The British Journal of Nutrition 102 (12):1703–8. doi: 10.1017/S0007114509992054.
   PubMed Web of Science ®Google Scholar
• Li, Y. 1988. Chinese name dictionary. Beijing, China: International Culture Press.
   Google Scholar
• Li, D., M. Schröder, and W. Vetter. 2012. Isolation of 6,9,12,15-hexadecatetraenoic fatty acid (16:4n-1) methyl ester from transesterified fish oil by HSCCC. Chromatographia 75 (1–2):1–6. doi: 10.1007/s10337-011-2155-7.
   Web of Science ®Google Scholar
• Liu, X. R., Z. Y. Deng, Y. W. Fan, J. Li, and Z. H. Liu. 2010. Mineral elements analysis of Momordica Charantiap seeds bv ICP—AES and fatty acid profile identification of seed oil by GC—MS. Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu 30:2265–8.
   PubMedGoogle Scholar
• Liu, Z., J. Zhu, Y. Niu, and Y. Yang. 2019. GC-MS analysis of liposoluble components in seeds of bitter gourd from different sources. Medicinal Plant 10:14–7.
   Google Scholar
• Lv, Y., X. Zhang, H. Zhang, S. Abbas, and E. Karangwa. 2013. The study of pH-dependent complexation between gelatin and gum arabic by morphology evolution and conformational transition. Food Hydrocolloids. 30 (1):323–32. doi: 10.1016/j.foodhyd.2012.06.007.
   Web of Science ®Google Scholar
• Madhani, H. D., and C. Guthrie. 1992. A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome. Cell 71 (5):803–17. doi: 10.1016/0092-8674(92)90556-R.
   PubMed Web of Science ®Google Scholar
• Martins, P. F., V. M. Ito, C. B. Batistella, and M. R. W. Maciel. 2006. Free fatty acid separation from vegetable oil deodorizer distillate using molecular distillation process. Separation and Purification Technology 48 (1):78–84. doi: 10.1016/j.seppur.2005.07.028.
   Web of Science ®Google Scholar
• Mendonça, F. M. R., A. E. Polloni, A. Junges, R. S. da Silva, A. F. Rubira, G. R. Borges, C. Dariva, and E. Franceschi. 2019. Encapsulation of neem (Azadirachta indica) seed oil in poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by SFEE technique. The Journal of Supercritical Fluids 152:104556. doi: 10.1016/j.supflu.2019.104556.
   Web of Science ®Google Scholar
• Noguchi, R., Y. Yasui, R. Suzuki, M. Hosokawa, K. Fukunaga, and K. Miyashita. 2001. Dietary effects of bitter gourd oil on blood and liver lipids of rats. Archives of Biochemistry and Biophysics 396 (2):207–12. doi: 10.1006/abbi.2001.2624.
   PubMed Web of Science ®Google Scholar
• Nyam, K. L., C. P. Tan, O. M. Lai, K. Long, and M. Y. B. C. Man. 2009. Physicochemical properties and bioactive compounds of selected seed oils. LWT - Food Science and Technology 42 (8):1396–403. doi: 10.1016/j.lwt.2009.03.006.
   Web of Science ®Google Scholar
• Pal, M., and M. Ghosh. 2012. Studies on comparative efficacy of α-linolenic acid and α-eleostearic acid on prevention of organic mercury-induced oxidative stress in kidney and liver of rat. Food and Chemical Toxicology : An International Journal Published for the British Industrial Biological Research Association 50 (3–4):1066–72. doi: 10.1016/j.fct.2011.12.042.
   PubMed Web of Science ®Google Scholar
• Paul, D., T. K. Dey, S. Mukherjee, M. Ghosh, and P. Dhar. 2014. Comparative prophylactic effects of α-eleostearic acid rich nano and conventional emulsions in induced diabetic rats. Journal of Food Science and Technology 51 (9):1724–36. doi: 10.1007/s13197-014-1257-2.
   PubMed Web of Science ®Google Scholar
• Paul, D., K. Manna, A. Sengupta, S. Mukherjee, S. Dey, P. K. Bag, and P. Dhar. 2019. A novel nanoformulation of α-eleostearic acid restores molecular pathogenesis of hypersensitivity. Nanomedicine (London, England) 14 (5):529–52. doi: 10.2217/nnm-2018-0450.
   PubMed Web of Science ®Google Scholar
• Prasad, P., P. Anjali, and R. V. Sreedhar. 2021. Plant-based stearidonic acid as sustainable source of omega-3 fatty acid with functional outcomes on human health. Critical Reviews in Food Science and Nutrition 61 (10):1725–37. − doi: 10.1080/10408398.2020.1765137.
   PubMed Web of Science ®Google Scholar
• Raina, K., D. Kumar, and R. Agarwal. 2016. Promise of bitter melon (Momordica charantia) bioactives in cancer prevention and therapy. Seminars in Cancer Biology 40–41:116–29. doi: 10.1016/j.semcancer.2016.07.002.
   PubMed Web of Science ®Google Scholar
• Rubio-Rodríguez, N., S. M. de Diego, S. Beltrán, I. Jaime, M. T. Sanz, and J. Rovira. 2008. Supercritical fluid extraction of the omega-3 rich oil contained in hake (Merluccius capensis–Merluccius paradoxus) by-products: Study of the influence of process parameters on the extraction yield and oil quality. The Journal of Supercritical Fluids 47 (2):215–26. doi: 10.1016/j.supflu.2008.07.007.
   Web of Science ®Google Scholar
• Saha, S. S., A. Chakraborty, S. Ghosh, and M. Ghosh. 2012. Comparative study of hypocholesterolemic and hypolipidemic effects of conjugated linolenic acid isomers against induced biochemical perturbations and aberration in erythrocyte membrane fluidity. European Journal of Nutrition 51 (4):483–95. doi: 10.1007/s00394-011-0233-0.
   PubMed Web of Science ®Google Scholar
• Saha, S. S., and M. Ghosh. 2012. Antioxidant and anti-inflammatory effect of conjugated linolenic acid isomers against streptozotocin-induced diabetes. The British Journal of Nutrition 108 (6):974–83. doi: 10.1017/S0007114511006325.
   PubMed Web of Science ®Google Scholar
• Saha, S. S., M. Patra, and M. Ghosh. 2012. In vitro antioxidant study of vegetable oils containing conjugated linolenic acid isomers. LWT - Food Science and Technology 46 (1):10–5. doi: 10.1016/j.lwt.2011.11.008.
   Web of Science ®Google Scholar
• Saini, R. K., and Y. S. Keum. 2017. Characterization of nutritionally important phytoconstituents in bitter melon (Momordica charantia L.) fruits by HPLC–DAD and GC–MS. Journal of Food Measurement and Characterization 11 (1):119–25. doi: 10.1007/s11694-016-9378-0.
   Web of Science ®Google Scholar
• Shuai, X., H. Wang, R. Zhang, N. Yang, M. Wu, S. Ye, Y. Li, and J. He. 2022. Process optimization of enrichment of α-eleostearic acid in balsam pear seed oil by urea encapsulation. China Oils and Fats 4:72–6. doi: 10.19902/j.cnki.zgyz.1003-7969.210302.
   Google Scholar
• Sun, X., L. Zhang, P. Li, B. Xu, F. Ma, Q. Zhang, and W. Zhang. 2015. Fatty acid profiles based adulteration detection for flaxseed oil by gas chromatography mass spectrometry. LWT - Food Science and Technology 63 (1):430–6. doi: 10.1016/j.lwt.2015.02.023.
   Web of Science ®Google Scholar
• Suzuki, R., R. Noguchi, T. Ota, M. Abe, K. Miyashita, and T. Kawada. 2001. Cytotoxic effect of conjugated trienoic fatty acids on mouse tumor and human monocytic leukemia cells. Lipids 36 (5):477–82. doi: 10.1007/s11745-001-0746-0.
   PubMed Web of Science ®Google Scholar
• Tsuzuki, T., Y. Tokuyama, M. Igarashi, and T. Miyazawa. 2004. Tumor growth suppression by alpha-eleostearic acid, a linolenic acid isomer with a conjugated triene system, via lipid peroxidation. Carcinogenesis 25 (8):1417–25. doi: 10.1093/carcin/bgh109.
   PubMed Web of Science ®Google Scholar
• Tsuyoshi, T., T. Yoshiko, I. Miki, and M. Teruo. 2004. Tumor growth suppression by α-eleostearic acid, a linolenic acid isomer with a conjugated triene system, via lipid peroxidation. Carcinogenesis 25 (8):1417–1425. doi: 10.1093/carcin/bgh109.
   PubMed Web of Science ®Google Scholar
• Umamaheshwari, P., and P. D. S. Reddy. 2016. Effect of operating parameters on extraction of oil from bitter gourd seeds: A kinetic and thermodynamic study. International Journal of Science and Research 5:1243–6.
   Google Scholar
• Vázquez, L., and C. C. Akoh. 2010. Fractionation of short and medium chain fatty acid ethyl esters from a blend of oils via ethanolysis and short-path distillation. Journal of the American Oil Chemists’ Society 87 (8):917–28. doi: 10.1007/s11746-010-1571-4.
   Web of Science ®Google Scholar
• Wang, S., Z. Li, G. Yang, C. T. Ho, and S. Li. 2017. Momordica charantia: A popular health-promoting vegetable with multifunctionality. Food & Function 8 (5):1749–62. doi: 10.1039/c6fo01812b.
   PubMed Web of Science ®Google Scholar
• Wu, D., Z. Deng, and Y. Yu. 2006. GC determination of bitter melon seeds fatty acids. Food Science and Technology 9:227–229. doi: 10.13684/j.cnki.spkj.2006.09.078.
   Google Scholar
• Xu, Y., C. Shi, Q. Wu, Z. Zheng, P. Liu, G. Li, X. Peng, and X. Xia. 2017. Antimicrobial activity of punicalagin against Staphylococcus aureus and its effect on biofilm formation. Foodborne Pathogens and Disease 14 (5):282–7. doi: 10.1089/fpd.2016.2226.
   PubMed Web of Science ®Google Scholar
• Xu, L., H. L. Wang, C. Y. Wu, M. H. Zhou, and Y. Y. Wang. 2014. Study on optimization of extraction technology for bitter melon seed oil by supercritical CO2. Applied Mechanics and Materials 618:354–61. doi: 10.4028/www.scientific.net/AMM.618.354.
   Google Scholar
• Xu, Y., L. Xu, X. T. Chen, P. Sun, Q. Guo, and H. L. Wang. 2016. Bitter melon seed oil may reduce the adiposity through the hypothalamus mTOR signaling in mice fed a high fat diet. Journal of Nutrition & Intermediary Metabolism 6:16–21. doi: 10.1016/j.jnim.2016.04.003.
   Google Scholar
• Yasui, Y., M. Hosokawa, T. Sahara, R. Suzuki, S. Ohgiya, H. Kohno, T. Tanaka, and K. Miyashita. 2005. Bitter gourd seed fatty acid rich in 9c,11t,13t-conjugated linolenic acid induces apoptosis and up-regulates the GADD45, p53 and PPARgamma in human colon cancer Caco-2 cells. Prostaglandins, Leukotrienes, and Essential Fatty Acids 73 (2):113–9. doi: 10.1016/j.plefa.2005.04.013.
   PubMed Web of Science ®Google Scholar
• Yoshime, L. T., I. L. Melo, J. A. G. Sattler, E. B. T. de Carvalho, and J. Mancini-Filho. 2016. Bitter gourd (Momordica charantia L.) seed oil as a naturally rich source of bioactive compounds for nutraceutical purposes. Nutrire 41 (1):7. doi: 10.1186/s41110-016-0013-y.
   Google Scholar
• Yu, H. P., F. C. Liu, C. Y. Lin, A. Umoro, J. Trousil, T. L. Hwang, and J. Y. Fang. 2020. Suppression of neutrophilic inflammation can be modulated by the droplet size of anti-inflammatory nanoemulsions. Nanomedicine (London, England) 15 (8):773–91. doi: 10.2217/nnm-2019-0407.
   PubMed Web of Science ®Google Scholar
• Zhang, F., Y. Pan, and K. Yang. 2011. GC-MS determination of bitter melon seeds oil fatty acids. Cereal and Food Industry 18 (3):19–22. doi: 10.3969/j.issn.1672-5026.2011.03.007.
   Google Scholar
• Zhuo, R. J., F. Wang, X. H. Zhang, J. J. Zhang, J. Xu, W. Dong, and Z. Q. Zou. 2014. Α-eleostearic acid inhibits growth and induces apoptosis in breast cancer cells via HER2/HER3 signaling . Molecular Medicine Reports 9 (3):993–8. doi: 10.3892/mmr.2014.1892.
   PubMed Web of Science ®Google Scholar
• Zubair, M. F., O. Atolani, S. O. Ibrahim, O. S. Oguntoye, H. A. Abdulrahim, R. A. Oyegoke, and G. A. Olatunji. 2018. Chemical and biological evaluations of potent antiseptic cosmetic products obtained from Momordica charantia seed oil. Sustainable Chemistry and Pharmacy 9:35–41. doi: 10.1016/j.scp.2018.05.005.
   Web of Science ®Google Scholar