Microalgae protein digestibility: How to crack open the black box?

References

• Abiusi, F., G. Sampietro, G. Marturano, N. Biondi, L. Rodolfi, M. D’Ottavio, and M. R. Tredici. 2014. Growth, photosynthetic efficiency, and biochemical composition of Tetraselmis suecica F&M-M33 grown with LEDs of different colors. Biotechnology and Bioengineering 111 (5):956–64. doi: 10.1002/bit.25014.
   PubMed Web of Science ®Google Scholar
• Agboola, J. O., E. Teuling, P. A. Wierenga, H. Gruppen, and J. W. Schrama. 2019. Cell wall disruption: An effective strategy to improve the nutritive quality of microalgae in African catfish (Clarias gariepinus). Aquaculture Nutrition 25:783–97. doi: 10.1111/anu.12896.
   Web of Science ®Google Scholar
• Agengo, F. B., A. N. Onyango, C. A. Serrem, and J. Okoth. 2020. Efficacy of compositing with snail meat powder on protein nutritional quality of sorghum-wheat buns using a rat bioassay. Journal of the Science of Food and Agriculture 100 (7):2963–70. doi: 10.1002/jsfa.10324.
   PubMed Web of Science ®Google Scholar
• Alhattab, M., A. Kermanshahi-Pour, and M. S. L. Brooks. 2019. Microalgae disruption techniques for product recovery: Influence of cell wall composition. Journal of Applied Phycology 31:61–88. doi: 10.1007/s10811-018-1560-9.
   Web of Science ®Google Scholar
• Allied Market Research. 2022. https://www.alliedmarketresearch.com/microalgae-market-A13419. Accessed January 30, 2022.
   Google Scholar
• Altmann, B. A., C. Neumann, S. Velten, F. Liebert, and D. Mörlein. 2018. Meat quality derived from high inclusion of a micro-alga or insect meal as an alternative protein source in poultry diets: A pilot study. Foods 7:34.
   PubMed Web of Science ®Google Scholar
• AOAC. 2000. Official methods of analysis of the Association of Official Analytical Chemists International. 17th Edition. Section 45.3.06 (AOAC Official Method 991.29, True Protein Digestibility of Foods and Food Ingredients, Rat Bioassay); Journal of AOAC International: Gaithersburg, MD, USA.
   Google Scholar
• AOAC. 2005. Pepsin digestibility of animal protein feeds. In Official methods of analysis of AOAC International, 18th ed., 39–40. Gaithersburg, MD: AOAC International.
   Google Scholar
• Araújo, R., F. Vázquez Calderón, J. Sánchez López, I. C. Azevedo, A. Bruhn, S. Fluch, M. Garcia Tasende, F. Ghaderiardakani, T. Ilmjärv, and M. Laurans. 2021. Current status of the algae production industry in Europe: An emerging sector of the blue bioeconomy. Frontiers in Marine Science 7:1247. doi: 10.3389/fmars.2020.626389.
   Web of Science ®Google Scholar
• Araya, M., S. García, J. Rengel, S. Pizarro, and G. Álvarez. 2021. Determination of free and protein amino acid content in microalgae by HPLC-DAD with pre-column derivatization and pressure hydrolysis. Marine Chemistry 234:103999. doi: 10.1016/j.marchem.2021.103999.
   Web of Science ®Google Scholar
• Barbé, F., O. Ménard, Y. L. Gouar, C. Buffière, M.-H. Famelart, B. Laroche, S. L. Feunteun, D. Rémond, and D. Dupont. 2014. Acid and rennet gels exhibit strong differences in the kinetics of milk protein digestion and amino acid bioavailability. Food Chemistry 143:1–8. doi: 10.1016/j.foodchem.2013.07.100.
   PubMed Web of Science ®Google Scholar
• Batista, A. P., A. Niccolai, I. Bursic, I. Sousa, A. Raymundo, L. Rodolfi, N. Biondi, and M. R. Tredici. 2019. Microalgae as functional ingredients in savory food products: Application to wheat crackers. Foods 8:611. doi: 10.3390/foods8120611.
   PubMed Web of Science ®Google Scholar
• Batista, A. P., A. Niccolai, P. Fradinho, S. Fragoso, I. Bursic, L. Rodolfi, N. Biondi, M. R. Tredici, I. Sousa, and A. Raymundo. 2017. Microalgae biomass as an alternative ingredient in cookies: Sensory, physical and chemical properties, antioxidant activity and in vitro digestibility. Algal Research 26:161–71. doi: 10.1016/j.algal.2017.07.017.
   Google Scholar
• Batista, A. P., L. Ambrosano, S. Graça, C. Sousa, P. Marques, B. Ribeiro, E. P. Botrel, P. Castro Neto, and L. Gouveia. 2015. Combining urban wastewater treatment with biohydrogen production - An integrated microalgae-based approach. Bioresource Technology 184:230–5. doi: 10.1016/j.biortech.2014.10.064.
   PubMed Web of Science ®Google Scholar
• Batista, S., M. Pintado, A. Marques, H. Abreu, J. L. Silva, F. Jessen, F. Tulli, and L. M. P. Valente. 2020. Use of technological processing of seaweed and microalgae as strategy to improve their apparent digestibility coefficients in European seabass (Dicentrarchus labrax) juveniles. Journal of Applied Phycology 32:3429–46. doi: 10.1007/s10811-020-02185-2.
   Web of Science ®Google Scholar
• Baune, M. C., A. L. Jeske, A. Profeta, S. Smetana, K. Broucke, G. Van Royen, M. Gibis, J. Weiss, and N. Terjung. 2021. Effect of plant protein extrudates on hybrid meatballs – Changes in nutritional composition and sustainability. Future Foods 4:100081.
   Google Scholar
• Becker, E. W. 2007. Micro-algae as a source of protein. Biotechnology Advances 25 (2):207–10. doi: 10.1016/j.biotechadv.2006.11.002.
   PubMed Web of Science ®Google Scholar
• Bernaerts, T. M. M., L. Gheysen, I. Foubert, M. E. Hendrickx, and A. M. Van Loey. 2019. Evaluating microalgal cell disruption upon ultra high pressure homogenization. Algal Research 42:101616.
   Google Scholar
• Bernaerts, T. M. M., H. Verstreken, C. Dejonghe, L. Gheysen, I. Foubert, T. Grauwet, and A. M. Van Loey. 2020. Cell disruption of Nannochloropsis sp. improves in vitro bioaccessibility of carotenoids and ω3-LC-PUFA. Journal of Functional Foods 65:103770.
   Web of Science ®Google Scholar
• Bohn, T., F. Carriere, L. Day, A. Deglaire, L. Egger, D. Freitas, M. Golding, S. Le Feunteun, A. Macierzanka, O. Menard, et al. 2018. Correlation between in vitro and in vivo data on food digestion. What can we predict with static in vitro digestion models? Critical Reviews in Food Science and Nutrition 58:2239–61.
   PubMed Web of Science ®Google Scholar
• Boisen, S., and J. A. Fernández. 1997. Prediction of the total tract digestibility of energy in feedstuffs and pig diets by in vitro analyses. Animal Feed Science and Technology 68:277–86.
   Web of Science ®Google Scholar
• Booth, M. A., and I. Pirozzi. 2021. The digestibility of raw materials by barramundi Lates calcarifer: Emphasis on the effect of inclusion rate on the digestibility of soybean meal and soy protein concentrate. Animal Feed Science and Technology 273:114800.
   Web of Science ®Google Scholar
• Boukid, F., and M. Castellari. 2021. Food and beverages containing algae and derived ingredients launched in the market from 2015 to 2019: A front-of-pack labeling perspective with a special focus on Spain. Foods 10:173.
   PubMed Web of Science ®Google Scholar
• Boukid, F., and M. Gagaoua. 2022. Vegan egg: A future-proof food ingredient? Foods 11:161.
   PubMed Web of Science ®Google Scholar
• Boukid, F., C. M. Rosell, S. Rosene, S. Bover-Cid, and M. Castellari. 2021. Non-animal proteins as cutting-edge ingredients to reformulate animal-free foodstuffs: Present status and future perspectives. Critical Reviews in Food Science and Nutrition 137:1–31.
   Google Scholar
• Brodkorb, A., L. Egger, M. Alminger, P. Alvito, R. Assunção, S. Ballance, T. Bohn, C. Bourlieu-Lacanal, R. Boutrou, F. Carrière, et al. 2019. INFOGEST static in vitro simulation of gastrointestinal food digestion. Nature Protocols 14:991–1014.
   PubMed Web of Science ®Google Scholar
• Burks, A. W., L. W. Williams, W. Thresher, C. Connaughton, G. Cockrell, and R. M. Helm. 1992. Allergenicity of peanut and soybean extracts altered by chemical or thermal denaturation in patients with atopic dermatitis and positive food challenges. The Journal of Allergy and Clinical Immunology 90 (6 Pt 1):889–97. doi: 10.1016/0091-6749(92)90461-a.
   PubMed Web of Science ®Google Scholar
• Butts, C. A., J. A. Monro, and P. J. Moughan. 2012. In vitro determination of dietary protein and amino acid digestibility for humans. British Journal of Nutrition 108:282–86.
   Web of Science ®Google Scholar
• Callejo-López, J. A., M. Ramírez, D. Cantero, and J. Bolívar. 2020. Versatile method to obtain protein- and/or amino acid-enriched extracts from fresh biomass of recalcitrant microalgae without mechanical pretreatment. Algal Research 50:102010.
   Google Scholar
• Caporgno, M. P., and A. Mathys. 2018. Trends in microalgae incorporation into innovative food products with potential health benefits. Frontiers in Nutrition 5:85.
   PubMed Web of Science ®Google Scholar
• Cavonius, L. R., E. Albers, and I. Undeland. 2015. pH-shift processing of Nannochloropsis oculata microalgal biomass to obtain a protein-enriched food or feed ingredient. Algal Research 11:95–102.
   Google Scholar
• Cavonius, L. R., E. Albers, and I. Undeland. 2016. In vitro bioaccessibility of proteins and lipids of pH-shift processed Nannochloropsis oculata microalga. Food & Function 7 (4):2016–24. doi: 10.1039/c5fo01144b.
   PubMed Web of Science ®Google Scholar
• Cerri, R., A. Niccolai, G. Cardinaletti, F. Tulli, F. Mina, E. Daniso, T. Bongiorno, G. Chini Zittelli, N. Biondi, M. R. Tredici, et al. 2021. Chemical composition and apparent digestibility of a panel of dried microalgae and cyanobacteria biomasses in rainbow trout (Oncorhynchus mykiss). Aquaculture 544:737075.
   Web of Science ®Google Scholar
• Changi, S. M., J. L. Faeth, N. Mo, and P. E. Savage. 2015. Hydrothermal reactions of biomolecules relevant for microalgae liquefaction. Industrial and Engineering Chemistry Research 54:11733–58.
   Web of Science ®Google Scholar
• Choi, Y. K., H. J. Kim, R. S. Kumaran, H. J. Song, K. G. Song, K. J. Kim, S. H. Lee, Y. H. Yang, and H. J. Kim. 2017. Enhanced growth and total fatty acid production of microalgae under various lighting conditions induced by flashing light. Engineering in Life Sciences 17:976.
   PubMed Web of Science ®Google Scholar
• Chronakis, I. S., and M. Madsen. 2011. Algal proteins. In Handbook of food proteins, eds. G. O. Phillips and P. A. Williams, 353–394. Sawston, UK: Woodhead Publishing Series in Food Sciences, Technology and Nutrition.
   Google Scholar
• Coelho, D., P. A. Lopes, V. Cardoso, P. Ponte, J. Brás, M. S. Madeira, C. M. Alfaia, N. M. Bandarra, C. M. G. A. Fontes, and J. A. M. Prates. 2020. A two‐enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets. Journal of Animal Physiology and Animal Nutrition (Berlin) 104:310.
   Google Scholar
• Colla, E., A. L. L. Menegotto, D. L. Kalschne, R. A. da Silva-Buzanello, C. Canan, and D. A. Drunkler. 2020. Microalgae: A new and promising source of food. In Handbook of algal science, technology and medicine, 507–18. London: Academic Press.
   Google Scholar
• Conde, T. A., B. F. Neves, D. Couto, T. Melo, B. Neves, M. Costa, J. Silva, P. Domingues, and M. R. Domingues. 2021. Microalgae as sustainable bio-factories of healthy lipids: Evaluating fatty acid content and antioxidant activity. Marine Drugs 19:357.
   PubMed Web of Science ®Google Scholar
• Coronado-Reyes, J. A., J. A. Salazar-Torres, B. Juárez-Campos, and J. C. González-Hernández. 2020. Chlorella vulgaris, a microalgae important to be used in Biotechnology: A review. Food Science and Technology 42:e37320.
   Web of Science ®Google Scholar
• Cruz, G., M. Oliveira, N. Costa, C. Pires, R. Cruz, and M. Moreira. 2005. Comparação entre a digestibilidade proteina in vitro e in vivo de diferentes cultivares de feijão (Phaseolus vulgaris L.) armazenados por 30 dias. Alimentos e Nutrição Araraquara 16:265–71.
   Google Scholar
• D’Hondt, E., J. Martín-Juárez, S. Bolado, J. Kasperoviciene, J. Koreiviene, S. Sulcius, K. Elst, and L. Bastiaens. 2017. Cell disruption technologies. In Microalgae-based biofuels and bioproducts: From feedstock cultivation to end-products, ed. C. Gonzalez-Fernandez and R. Muñoz, 133–54. Sawston, United Kingdom: Woodhead Publishing.
   Google Scholar
• Deglaire, A., and P. J. Moughan. 2012. Animal models for determining amino acid digestibility in humans – A review. British Journal of Nutrition 108:S273–S281. doi: 10.1017/S0007114512002346.
   PubMed Web of Science ®Google Scholar
• Deglaire, A., C. Bos, D. Tomé, and P. J. Moughan. 2009. Ileal digestibility of dietary protein in the growing pig and adult human. The British Journal of Nutrition 102 (12):1752–9. doi: 10.1017/S0007114509991267.
   PubMed Web of Science ®Google Scholar
• Devi, M. A., G. Subbulakshmi, K. M. Devi, and L. V. Venkataraman. 1981. Studies on the proteins of mass-cultivated, blue-green alga (Spirulina platensis). Journal of Agricultural and Food Chemistry 29 (3):522–5. doi: 10.1021/jf00105a022.
   PubMed Web of Science ®Google Scholar
• Devi, S., A. Varkey, M. S. Sheshshayee, T. Preston, and A. V. Kurpad. 2018. Measurement of protein digestibility in humans by a dual-tracer method. The American Journal of Clinical Nutrition 107 (6):984–91. doi: 10.1093/ajcn/nqy062.
   PubMed Web of Science ®Google Scholar
• Doucha, J., and K. Lívanský. 2008. Influence of processing parameters on disintegration of Chlorella cells in various types of homogenizers. Applied Microbiology and Biotechnology 81 (3):431–40. doi: 10.1007/s00253-008-1660-6.
   PubMed Web of Science ®Google Scholar
• Dufour, C., M. Loonis, M. Delosière, C. Buffière, N. Hafnaoui, V. Santé-Lhoutellier, and D. Rémond. 2018. The matrix of fruit & vegetables modulates the gastrointestinal bioaccessibility of polyphenols and their impact on dietary protein digestibility. Food Chemistry 240:314–22. doi: 10.1016/j.foodchem.2017.07.104.
   PubMed Web of Science ®Google Scholar
• Ellner, C., B. Martínez-Vallespín, E. M. Saliu, J. Zentek, and I. Röhe. 2021. Effects of cereal and protein source on performance, apparent ileal protein digestibility and intestinal characteristics in weaner piglets. Archives of Animal Nutrition 75 (4):263–77. doi: 10.1080/1745039X.2021.1958647.
   PubMed Web of Science ®Google Scholar
• Enzing, C., M. Ploeg, M. Barbosa, and L. Sijtsma. 2014. Microalgae-based products for the food and feed sector: An outlook for Europe. Luxembourg: Publications Office of the European Union.
   Google Scholar
• Ermis, H., and M. Altinbas. 2020. Effect of salinity on mixed microalgae grown in anaerobic liquid digestate. Water and Environment Journal 34:820–30. doi: 10.1111/wej.12580.
   Web of Science ®Google Scholar
• FAO. 2013. Dietary protein quality evaluation in human nutrition Report of an FAO Expert Consultation. Rome: Food and Agriculture Organization of the United Nations.
   Google Scholar
• FAO/WHO. 1991. Protein quality evaluation: Report of the joint FAO/WO expert consultation. FAO Food and Nutrition. Paper, 51.
   Google Scholar
• FAO/WHO. 2007. Accessed February 9, 2023. https://apps.who.int/iris/handle/10665/43411.
   Google Scholar
• Foo, S. C., K. S. Khoo, C. W. Ooi, P. L. Show, N. M. H. Khong, and F. M. Yusoff. 2020. Meeting sustainable development goals: Alternative extraction processes for fucoxanthin in algae. Frontiers in Bioengineering and Biotechnology 8:546067. doi: 10.3389/fbioe.2020.546067.
   PubMed Web of Science ®Google Scholar
• Fradinho, P., A. Niccolai, R. Soares, L. Rodolfi, N. Biondi, M. R. Tredici, I. Sousa, and A. Raymundo. 2020. Effect of Arthrospira platensis (spirulina) incorporation on the rheological and bioactive properties of gluten-free fresh pasta. Algal Research 45:101743.
   Google Scholar
• Gan, Q., J. Jiang, X. Han, S. Wang, and Y. Lu. 2018. Engineering the chloroplast genome of oleaginous marine microalga nannochloropsis oceanica. Frontiers in Plant Science 9:439.
   PubMed Web of Science ®Google Scholar
• Gantt, E., and C. A. Lipschultz. 1974. Phycobilisomes of Porphyridium cruentum: Pigment analysis. Biochemistry 13 (14):2960–6. doi: 10.1021/bi00711a027.
   PubMed Web of Science ®Google Scholar
• Gauthier, S. F., C. Vachon, J. D. Jones, and L. Savoie. 1982. Assessment of protein digestibility by in vitro enzymatic hydrolysis with simultaneous dialysis. The Journal of Nutrition 112 (9):1718–25. doi: 10.1093/jn/112.9.1718.
   PubMed Web of Science ®Google Scholar
• Gerde, J. A., T. Wang, L. Yao, S. Jung, L. A. Johnson, and B. Lamsal. 2013. Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass. Algal Research 2:145–53.
   Google Scholar
• Gerken, H. G., B. Donohoe, and E. P. Knoshaug. 2013. Enzymatic cell wall degradation of Chlorella vulgaris and other microalgae for biofuels production. Planta 237 (1):239–53. doi: 10.1007/s00425-012-1765-0.
   PubMed Web of Science ®Google Scholar
• GfE Society of Nutrition Physiology. 2006. Recommendations for the supply of energy and nutrients to pigs energy and nutrients requirements for livestock. Committee for Requirement Standards of the Society of Nutrition Physiology. Frankfurt am Main, Germany: DLG-Verlags GmbH.
   Google Scholar
• Glencross, B., D. Blyth, N. Wade, and S. Arnold. 2018. Critical variability exists in the digestible value of raw materials fed to black tiger shrimp, Penaeus monodon: The characterisation and digestibility assessment of a series of research and commercial raw materials. Aquaculture 495:214–21.
   Web of Science ®Google Scholar
• Gong, Y., H. A. D. S. Guterres, M. Huntley, M. Sørensen, and V. Kiron. 2018. Digestibility of the defatted microalgae Nannochloropsis sp. and Desmodesmus sp. when fed to Atlantic salmon, Salmo salar. Aquaculture Nutrition 24:56–64.
   Web of Science ®Google Scholar
• Gorissen, S. H. M., J. J. R. Crombag, J. M. G. Senden, W. A. H. Waterval, J. Bierau, L. B. Verdijk, and L. v. Loon. 2018. Protein content and amino acid composition of commercially available plant-based protein isolates. Amino Acid 50:1685–95.
   PubMed Web of Science ®Google Scholar
• Graziani, G., S. Schiavo, M. A. Nicolai, S. Buono, V. Fogliano, G. Pinto, and A. Pollio. 2013. Microalgae as human food: Chemical and nutritional characteristics of the thermo-acidophilic microalga Galdieria sulphuraria. Food & Function 4 (1):144–52. doi: 10.1039/c2fo30198a.
   PubMed Web of Science ®Google Scholar
• Grela, E. R., B. Kiczorowska, W. Samolińska, J. Matras, P. Kiczorowski, W. Rybiński, and E. Hanczakowska. 2017. Chemical composition of leguminous seeds: Part I—content of basic nutrients, amino acids, phytochemical compounds, and antioxidant activity. European Food Research and Technology 243:1385–95.
   Web of Science ®Google Scholar
• Grossmann, L., S. Ebert, J. Hinrichs, and J. Weiss. 2018. Production of protein-rich extracts from disrupted microalgae cells: Impact of solvent treatment and lyophilization. Algal Research 36:67–76.
   Google Scholar
• Guccione, A., N. Biondi, G. Sampietro, L. Rodolfi, N. Bassi, and M. R. Tredici. 2014. Chlorella for protein and biofuels: From strain selection to outdoor cultivation in a Green Wall Panel photobioreactor. Biotechnology for Biofuels 7:84.
   PubMed Web of Science ®Google Scholar
• Guillin, F. M., C. Gaudichon, L. Guérin-Deremaux, C. Lefranc-Millot, G. Airinei, N. Khodorova, R. Benamouzig, P. H. Pomport, J. Martin, and J. Calvez. 2022. Real ileal amino acid digestibility of pea protein compared to casein in healthy humans: A randomized trial. The American Journal of Clinical Nutrition 115 (2):353–63. doi: 10.1093/ajcn/nqab354.
   PubMed Web of Science ®Google Scholar
• Guillin, F. M., C. Gaudichon, L. Guérin-Deremaux, C. Lefranc-Millot, D. Azzout-Marniche, N. Khodorova, and J. Calvez. 2021. Multi-criteria assessment of pea protein quality in rats: A comparison between casein, gluten and pea protein alone or supplemented with methionine. The British Journal of Nutrition 125 (4):389–97. doi: 10.1017/S0007114520002883.
   PubMed Web of Science ®Google Scholar
• Guiry, M. D., and G. M. Guiry. 2022. https://www.algaebase.org.
   Google Scholar
• Hedenskog, G. 1978. Properties and composition op single-cell protein, influence op processing. In Biochemical aspects of new protein food, 73–88.
   Google Scholar
• Hedenskog, G., L. Enebo, J. Vendlová, and B. Prokeš. 1969. Investigation of some methods for increasing the digestibility in vitro of microalgae. Biotechnology and Bioengineering 11 (1):37–51. doi: 10.1002/bit.260110104.
   PubMed Web of Science ®Google Scholar
• Hendriks, W. H., Van Baal, J., and Bosch, G. 2012. Ileal and faecal protein digestibility measurement in humans and other non-ruminants - A comparative species view. British Journal of Nutrition 108:S247–S257.
   PubMed Web of Science ®Google Scholar
• Hoa, N. T. Q., L. N. Tam, L. V. T. Phu, T. Thai, D. C. Van Nguyen, and D. T. A. Dao. 2019. Manufacturing process, in vivo and in vitro digestibility assessment of an enteral feeding product hydrolyzed from locally available ingredients using commercial enzymes. Process 7:347.
   Web of Science ®Google Scholar
• Hori, K., T. Ueno-Mohri, T. Okita, and G. Ishibashi. 1990. Chemical composition, in vitro protein digestibility and in vitro available iron of blue green alga, Nostoc commune. Plant Foods for Human Nutrition 40:223–9.
   PubMed Web of Science ®Google Scholar
• Huang, Y., S. Qin, D. Zhang, L. Li, and Y. Mu. 2016. Evaluation of cell disruption of chlorella vulgaris by pressure-assisted ozonation and ultrasonication. Energies 9:173.
   Web of Science ®Google Scholar
• Hughes, G. J., D. J. Ryan, R. Mukherjea, and C. S. Schasteen. 2011. Protein digestibility-corrected amino acid scores (PDCAAS) for soy protein isolates and concentrate: Criteria for evaluation. Journal of Agricultural and Food Chemistry 59 (23):12707–12. doi: 10.1021/jf203220v.
   PubMed Web of Science ®Google Scholar
• Hw Hsu, D. 1977. A multienzyme technique for estimating protein digestibility. Journal of Food Science 42:1269–73.
   Web of Science ®Google Scholar
• James, K. A. C., C. A. Butts, J. P. Koolaard, H. E. Donaldson, M. F. Scott, and P. J. Moughan. 2002. The effect of feeding regimen on apparent and true ileal nitrogen digestibility for rats fed diets containing different sources of protein. Journal of the Science of Food and Agriculture 82:1050–60.
   Web of Science ®Google Scholar
• Janczyk, P., H. Franke, and W. B. Souffrant. 2007. Nutritional value of Chlorella vulgaris: Effects of ultrasonication and electroporation on digestibility in rats. Animal Feed Science and Technology 132:163–9.
   Web of Science ®Google Scholar
• Jepsen, P. M., C. V. Thoisen, T. Carron-Cabaret, A. Pinyol-Gallemí, S. L. Nielsen, and B. W. Hansen. 2019. Effects of salinity, commercial salts, and water type on cultivation of the cryptophyte microalgae Rhodomonas salina and the Calanoid Copepod Acartia tonsa. Journal of the World Aquaculture Society 50:104–18.
   Web of Science ®Google Scholar
• Kalia, S., and X. G. Lei. 2022. Dietary microalgae on poultry meat and eggs: Explained versus unexplained effects. Current Opinion in Biotechnology 75:102689. doi: 10.1016/j.copbio.2022.102689.
   PubMed Web of Science ®Google Scholar
• Kannaujiya, V. K., D. Kumar, V. Singh, and R. P. Sinha. 2021. Advances in phycobiliproteins research: Innovations and commercialization. In Natural bioactive compounds, 57–81. London: Academic Press.
   Google Scholar
• Ketnawa, S., and Y. Ogawa. 2019. Evaluation of protein digestibility of fermented soybeans and changes in biochemical characteristics of digested fractions. Journal of Functional Foods 52:640–7.
   Web of Science ®Google Scholar
• Khemiri, S., M. C. Nunes, R. J. B. Bessa, S. P. Alves, I. Smaali, and A. Raymundo. 2021. Technological feasibility of couscous-algae-supplemented formulae: Process description, nutritional properties and in vitro digestibility. Foods 10:3159.
   PubMed Web of Science ®Google Scholar
• Kibria, S., and I. H. Kim. 2019. Impacts of dietary microalgae (Schizochytrium JB5) on growth performance, blood profiles, apparent total tract digestibility, and ileal nutrient digestibility in weaning pigs. Journal of the Science of Food and Agriculture 99 (13):6084–8.
   PubMed Web of Science ®Google Scholar
• Komaki, H., M. Yamashita, Y. Niwa, Y. Tanaka, N. Kamiya, Y. Ando, and M. Furuse. 1998. The effect of processing of Chlorella vulgaris: K-5 on in vitro and in vivo digestibility in rats. Animal Feed Science and Technology 70:363–6.
   Web of Science ®Google Scholar
• Kong, F., and R. P. Singh. 2010. A human gastric simulator (HGS) to study food digestion in human stomach. Journal of Food Science 75:E627–E635.
   PubMed Web of Science ®Google Scholar
• Kose, A., and S. S. Oncel. 2015. Properties of microalgal enzymatic protein hydrolysates: Biochemical composition, protein distribution and FTIR characteristics. Biotechnology Reports (Amsterdam, Netherlands) 6:137–43. doi: 10.1016/j.btre.2015.02.005.
   PubMedGoogle Scholar
• Kose, A., M. O. Ozen, M. Elibol, and S. S. Oncel. 2017. Investigation of in vitro digestibility of dietary microalga Chlorella vulgaris and cyanobacterium Spirulina platensis as a nutritional supplement. 3:170. Biotech 7.
   Google Scholar
• Kousoulaki, K., T. Mørkøre, I. Nengas, R. K. Berge, and J. Sweetman. 2016. Microalgae and organic minerals enhance lipid retention efficiency and fillet quality in Atlantic salmon (Salmo salar L.). Aquaculture 451:47–57.
   Web of Science ®Google Scholar
• Koyande, A. K., K. W. Chew, K. Rambabu, Y. Tao, D. T. Chu, and P. L. Show. 2019. Microalgae: A potential alternative to health supplementation for humans. Food Science and Human Wellness 8:16–24.
   Google Scholar
• Kristinsson, H. G., and B. A. Rasco. 2000. Biochemical and functional properties of Atlantic salmon (Salmo salar) muscle proteins hydrolyzed with various alkaline proteases. Journal of Agricultural and Food Chemistry 48 (3):657–66. doi: 10.1021/jf990447v.
   PubMed Web of Science ®Google Scholar
• Lafarga, T., J. M. Fernández-Sevilla, C. González-López, and F. G. Acién-Fernández. 2020. Spirulina for the food and functional food industries. Food Research International (Ottawa, ON) 137:109356. doi: 10.1016/j.foodres.2020.109356.
   PubMed Web of Science ®Google Scholar
• Lamminen, M., A. Halmemies-Beauchet-Filleau, T. Kokkonen, S. Jaakkola, and A. Vanhatalo. 2019. Different microalgae species as a substitutive protein feed for soya bean meal in grass silage based dairy cow diets. Animal Feed Science and Technology 247:112–26.
   Web of Science ®Google Scholar
• Lee, J.-Y., S.-B. Cho, Y.-Y. Kim, and S.-J. Ohh. 2011. Effect of gamma irradiation on nutrient digestibility in SPF mini-pig. Radiation Physics and Chemistry 80 (1):123–4. doi: 10.1016/j.radphyschem.2010.08.004.
   Web of Science ®Google Scholar
• Lin, Y. H., and Y. T. Chen. 2022. Lactobacillus spp. fermented soybean meal partially substitution to fish meal enhances innate immune responses and nutrient digestibility of white shrimp (Litopenaeus vannamei) fed diet with low fish meal. Aquaculture 548:737634.
   Web of Science ®Google Scholar
• MacColl, R., and D. Guard-Friar. 1987. Phycobiliproteins. Boca Raton, UK: CRC Press.
   Google Scholar
• Manus, J., M. Millette, B. R. A. Uscanga, S. Salmieri, B. Maherani, and M. Lacroix. 2021. In vitro protein digestibility and physico-chemical properties of lactic acid bacteria fermented beverages enriched with plant proteins. Journal of Food Science 86 (9):4172–82. doi: 10.1111/1750-3841.15859.
   PubMed Web of Science ®Google Scholar
• Markou, G., D. Arapoglou, C. Eliopoulos, A. Balafoutis, R. Taddeo, A. Panara, and N. Thomaidis. 2019. Cultivation and safety aspects of Arthrospira platensis (Spirulina) grown with struvite recovered from anaerobic digestion plant as phosphorus source. Algal Research 44:101716.
   Google Scholar
• Marzo, F., F. I. Milagro, E. Urdaneta, J. Barrenetxe, and F. C. Ibañez. 2011. Extrusion decreases the negative effects of kidney bean on enzyme and transport activities of the rat small intestine. Journal of Animal Physiology and Animal Nutrition 95 (5):591–8. doi: 10.1111/j.1439-0396.2010.01088.x.
   PubMed Web of Science ®Google Scholar
• Massa, M., S. Buono, A. L. Langellotti, A. Martello, G. L. Russo, D. A. Troise, R. Sacchi, P. Vitaglione, and V. Fogliano. 2019. Biochemical composition and in vitro digestibility of Galdieria sulphuraria grown on spent cherry-brine liquid. New Biotechnology 53:9–15. doi: 10.1016/j.nbt.2019.06.003.
   PubMed Web of Science ®Google Scholar
• Mat, D. J. L., S. Le Feunteun, C. Michon, and I. Souchon. 2016. In vitro digestion of foods using pH-stat and the INFOGEST protocol: Impact of matrix structure on digestion kinetics of macronutrients, proteins and lipids. Food Research International 88:226–33.
   Web of Science ®Google Scholar
• Mata, T. M., M. Cameira, F. Marques, E. Santos, S. Badenes, L. Costa, V. V. Vieira, N. S. Caetano, and A. A. Martins. 2018. Carbon footprint of microalgae production in photobioreactor. Energy Procedia 153:432–7.
   Google Scholar
• Matos, J., C. Cardoso, N. M. Bandarra, and C. Afonso. 2017. Microalgae as healthy ingredients for functional food: A review. Food & Function 8 (8):2672–85. doi: 10.1039/c7fo00409e.
   PubMed Web of Science ®Google Scholar
• McInnes, E. F., and Mann, P. 2012. Background Lesions in Laboratory Animals. Saint Louis: Saunders Ltd.
   Google Scholar
• Ménard, O., T. Cattenoz, H. Guillemin, I. Souchon, A. Deglaire, D. Dupont, and D. Picque. 2014. Validation of a new in vitro dynamic system to simulate infant digestion. Food Chemistry 145:1039–45. doi: 10.1016/j.foodchem.2013.09.036.
   PubMed Web of Science ®Google Scholar
• Ménard, O., M. H. Famelart, A. Deglaire, Y. Le Gouar, S. Guérin, C. H. Malbert, and D. Dupont. 2018. Gastric emptying and dynamic in vitro digestion of drinkable yogurts: Effect of viscosity and composition. Nutrients 10:1308.
   PubMed Web of Science ®Google Scholar
• Mendes-Pinto, M. M., M. F. J. Raposo, J. Bowen, A. J. Young, and R. Morais. 2001. Evaluation of different cell disruption processes on encysted cells of Haematococcus pluvialis: Effects on astaxanthin recovery and implications for bioavailability. Journal of Applied Phycology 13:19–24.
   Web of Science ®Google Scholar
• Minekus, M., M. Alminger, P. Alvito, S. Ballance, T. Bohn, C. Bourlieu, F. Carrière, R. Boutrou, M. Corredig, and D. Dupont. 2014. A standardised static in vitro digestion method suitable for food-an international consensus. Food & Function 5:1113–24.
   PubMed Web of Science ®Google Scholar
• Minekus, M., P. Marteau, R. Havenaar, and J. i. Veld. 1995. A multicompartmental dynamic computer-controlled model simulating the stomach and small intestine. Alternatives to Laboratory Animals 23:197–209.
   Web of Science ®Google Scholar
• Miralles, B., R. d. Barrio, C. Cueva, I. Recio, and L. Amigo. 2018. Dynamic gastric digestion of a commercial whey protein concentrate. Journal of the Science of Food and Agriculture 98:1873–9.
   PubMed Web of Science ®Google Scholar
• Moheimani, N. R., A. Vadiveloo, J. M. Ayre, and J. R. Pluske. 2018. Nutritional profile and in vitro digestibility of microalgae grown in anaerobically digested piggery effluent. Algal Research 35:362–9.
   Google Scholar
• Morris, H. J., A. Almarales, O. Carrillo, and R. C. Bermúdez. 2008. Utilisation of Chlorella vulgaris cell biomass for the production of enzymatic protein hydrolysates. Bioresource Technology 99 (16):7723–9. doi: 10.1016/j.biortech.2008.01.080.
   PubMed Web of Science ®Google Scholar
• Muys, M., Y. Sui, B. Schwaiger, C. Lesueur, D. Vandenheuvel, P. Vermeir, and S. E. Vlaeminck. 2019. High variability in nutritional value and safety of commercially available Chlorella and Spirulina biomass indicates the need for smart production strategies. Bioresource Technology 275:247–57. doi: 10.1016/j.biortech.2018.12.059.
   PubMed Web of Science ®Google Scholar
• National Research Council. 2011. Nutrient requirements of fish and shrimp, 376. Washington: National Academy Press.
   Google Scholar
• Niccolai, A., G. Chini Zittelli, L. Rodolfi, N. Biondi, and M. R. Tredici. 2019a. Microalgae of interest as food source: Biochemical composition and digestibility. Algal Research 42:101617.
   Google Scholar
• Niccolai, A., M. Venturi, V. Galli, N. Pini, L. Rodolfi, N. Biondi, M. D’Ottavio, A. P. Batista, A. Raymundo, L. Granchi, et al. 2019b. Development of new microalgae-based sourdough “crostini”: Functional effects of Arthrospira platensis (spirulina) addition. Scientific Reports 9:19433.
   PubMed Web of Science ®Google Scholar
• Nicolás Carcelén, J., J. M. Marchante-Gayón, P. R. González, L. Valledor, M. J. Cañal, and J. I. G. Alonso. 2017. A cost-effective approach to produce 15N-labelled amino acids employing Chlamydomonas reinhardtii CC503. Microbial Cell Factories 16:1–8.
   PubMed Web of Science ®Google Scholar
• Nosworthy, M. G., and J. D. House. 2017. Factors influencing the quality of dietary proteins: Implications for pulses. Cereal Chemistry 94:49–57.
   Web of Science ®Google Scholar
• Passos, F., J. Carretero, and I. Ferrer. 2015. Comparing pretreatment methods for improving microalgae anaerobic digestion: Thermal, hydrothermal, microwave and ultrasound. Chemical Engineering Journal and the Biochemical Engineering Journal 279:667–72.
   Google Scholar
• Pedersen, B., and B. Eggum. 1983. Prediction of protein digestibility by an in vitro enzymatic pH-stat procedure. Z Tierphysiol Tierernahr Futtermittelkd 49:265–77.
   PubMedGoogle Scholar
• Pereira, H., J. Silva, T. Santos, K. N. Gangadhar, A. Raposo, C. Nunes, M. A. Coimbra, L. Gouveia, L. Barreira, and J. Varela. 2019. Nutritional potential and toxicological evaluation of tetraselmis sp. CTP4 microalgal biomass produced in industrial photobioreactors. Molecules 24:3192.
   PubMed Web of Science ®Google Scholar
• Phong, W., P. Show, C. Le, Y. Tao, J. Chang, and T. Ling. 2018. Improving cell disruption efficiency to facilitate protein release from microalgae using chemical and mechanical integrated method. Biochemical Engineering Journal 135:83–90.
   Web of Science ®Google Scholar
• Postma, P. R., E. Suarez-Garcia, C. Safi, K. Yonathan, G. Olivieri, M. J. Barbosa, R. H. Wijffels, and M. Eppink. 2017. Energy efficient bead milling of microalgae: Effect of bead size on disintegration and release of proteins and carbohydrates. Bioresource Technology 224:670–9. doi: 10.1016/j.biortech.2016.11.071.
   PubMed Web of Science ®Google Scholar
• Prabakaran, P., and A. D. Ravindran. 2011. A comparative study on effective cell disruption methods for lipid extraction from microalgae. Letters in Applied Microbiology 53 (2):150–4. doi: 10.1111/j.1472-765X.2011.03082.x.
   PubMed Web of Science ®Google Scholar
• Prüser, T. F., P. G. Braun, and C. Wiacek. 2021. Microalgae as a novel food. Potential and legal framework. Ernährungs Umschau 68:78–85.
   Web of Science ®Google Scholar
• Qazi, W. M., S. Ballance, K. Kousoulaki, A. K. Uhlen, D. M. M. Kleinegris, K. Skjånes, and A. Rieder. 2021. Protein enrichment of wheat bread with microalgae: Microchloropsis gaditana, Tetraselmis chui and Chlorella vulgaris. Foods 10:3078.
   PubMed Web of Science ®Google Scholar
• Reeves, P. G., F. H. Nielsen, and G. C. Fahey. 1993. AIN-93 purified diets for laboratory rodents: Final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. Journal of Nutrition 123:1939–51.
   PubMed Web of Science ®Google Scholar
• Ribeiro, M., C. Carvalho, V. Carnide, H. Guedes-Pinto, and G. Igrejas. 2011. Towards allelic diversity in the storage proteins of old and currently growing tetraploid and hexaploid wheats in Portugal. Genetic Resources and Crop Evolution 58:1051–73.
   Web of Science ®Google Scholar
• Rieder, A., N. K. Afseth, U. Böcker, S. H. Knutsen, B. Kirkhus, H. K. Mæhre, S. Ballance, and S. G. Wubshet. 2021. Improved estimation of in vitro protein digestibility of different foods using size exclusion chromatography. Food Chemistry 358:129830. doi: 10.1016/j.foodchem.2021.129830.
   PubMed Web of Science ®Google Scholar
• Rodolfi, L., N. Biondi, A. Guccione, N. Bassi, M. D’Ottavio, G. Arganaraz, and M. R. Tredici. 2017. Oil and eicosapentaenoic acid production by the diatom Phaeodactylum tricornutum cultivated outdoors in Green Wall Panel (GWP®) reactors. Biotechnology and Bioengineering 114 (10):2204–10. doi: 10.1002/bit.26353.
   PubMed Web of Science ®Google Scholar
• Rodríguez-Miranda, E., J. L. Guzmán, F. G. Acién, M. Berenguel, and A. Visioli. 2021. Indirect regulation of temperature in raceway reactors by optimal management of culture depth. Biotechnology and Bioengineering 118 (3):1186–98. doi: 10.1002/bit.27642.
   PubMed Web of Science ®Google Scholar
• Safi, C., L. Cabas Rodriguez, W. J. Mulder, N. Engelen-Smit, W. Spekking, L. A. M. Broek, G. van den, Olivieri, and L. Sijtsma. 2017. Energy consumption and water-soluble protein release by cell wall disruption of Nannochloropsis gaditana. Bioresource Technology 239:204–10.
   PubMed Web of Science ®Google Scholar
• Safi, C., C. Frances, A. V. Ursu, C. Laroche, C. Pouzet, C. Vaca-Garcia, and P. Y. Pontalier. 2015. Understanding the effect of cell disruption methods on the diffusion of chlorella vulgaris proteins and pigments in the aqueous phase. Algal Research 8:61–8. doi: 10.1016/j.algal.2015.01.002.
   Google Scholar
• Safi, C., A. V. Ursu, C. Laroche, B. Zebib, O. Merah, P. Y. Pontalier, and C. Vaca-Garcia. 2014. Aqueous extraction of proteins from microalgae: Effect of different cell disruption methods. Algal Research 3:61–5. doi: 10.1016/j.algal.2013.12.004.
   Google Scholar
• Sarker, P. K., M. M. Gamble, S. Kelson, and A. R. Kapuscinski. 2016. Nile tilapia (Oreochromis niloticus) show high digestibility of lipid and fatty acids from marine Schizochytrium sp. and of protein and essential amino acids from freshwater Spirulina sp. feed ingredients. Aquaculture Nutrition 22:109–19. doi: 10.1111/anu.12230.
   Web of Science ®Google Scholar
• Sarker, P. K., A. R. Kapuscinski, A. Y. Bae, E. Donaldson, A. J. Sitek, D. S. Fitzgerald, and O. F. Edelson. 2018. Towards sustainable aquafeeds: Evaluating substitution of fishmeal with lipid-extracted microalgal co-product (Nannochloropsis oculata) in diets of juvenile Nile tilapia (Oreochromis niloticus). PLoS One 13:e0201315. doi: 10.1371/journal.pone.0201315.
   Web of Science ®Google Scholar
• Sarker, P. K., A. R. Kapuscinski, B. McKuin, D. S. Fitzgerald, H. M. Nash, and C. Greenwood. 2020. Microalgae-blend tilapia feed eliminates fishmeal and fish oil, improves growth, and is cost viable. Scientific Reports 10:19328. doi: 10.1038/s41598-020-75289-x.
   PubMed Web of Science ®Google Scholar
• Sathasivam, R., R. Radhakrishnan, A. Hashem, and E. F. Abd Allah. 2019. Microalgae metabolites: A rich source for food and medicine. Saudi Journal of Biological Sciences 26 (4):709–22. doi: 10.1016/j.sjbs.2017.11.003.
   PubMed Web of Science ®Google Scholar
• Satterlee, L. D., H. F. Marshall, and J. M. Tennyson. 1979. Measuring protein quality. Journal of the American Oil Chemists’ Society 56 (3):103–9. doi: 10.1007/BF02671431.
   PubMed Web of Science ®Google Scholar
• Schulze, C., M. Wetzel, J. Reinhardt, M. Schmidt, L. Felten, and S. Mundt. 2016. Screening of microalgae for primary metabolites including β-glucans and the influence of nitrate starvation and irradiance on β-glucan production. Journal of Applied Phycology 28:2719–25. doi: 10.1007/s10811-016-0812-9.
   Web of Science ®Google Scholar
• Schwenzfeier, A., P. A. Wierenga, and H. Gruppen. 2011. Isolation and characterization of soluble protein from the green microalgae Tetraselmis sp. Bioresource Technology 102 (19):9121–7. doi: 10.1016/j.biortech.2011.07.046.
   PubMed Web of Science ®Google Scholar
• Shewry, P. R., and S. J. Hey. 2015. The contribution of wheat to human diet and health. Food and Energy Security 4 (3):178–202. doi: 10.1002/fes3.64.
   PubMed Web of Science ®Google Scholar
• Shewry, P. R., J. A. Napier, and A. S. Tatham. 1995. Seed storage proteins: Structures and biosynthesis. The Plant Cell 7 (7):945–56. doi: 10.1105/tpc.7.7.945.
   PubMed Web of Science ®Google Scholar
• Siccardi, A. 2006. Daily digestible protein and energy requirements for growth and maintenance of sub-adult pacific white shrimp (Litopenaeus vannamei). Dissertation.
   Google Scholar
• Skrede, A., L. T. Mydland, O. Ahlstrem, K. I. Reitan, H. R. Gislered, and M. Overland. 2011. Evaluation of microalgae as sources of digestible nutrients for monogastric animals. Journal of Animal and Feed Sciences 20:131–42. doi: 10.22358/jafs/66164/2011.
   Web of Science ®Google Scholar
• Soares, M., P. C. Rezende, N. M. Corrêa, J. S. Rocha, M. A. Martins, T. C. Andrade, D. M. Fracalossi, and F. d. NascimentoVieira. 2020. Protein hydrolysates from poultry by-product and swine liver as an alternative dietary protein source for the Pacific white shrimp. Aquaculture Reports 17:100344. doi: 10.1016/j.aqrep.2020.100344.
   Web of Science ®Google Scholar
• Souci, S. W., W. Fachmann, and H. Kraut. 2008. Food composition and nutrition tables. Boca Raton, UK: Tyaler & Francis A CRC Press Book.
   Google Scholar
• Sui, Y., and S. E. Vlaeminck. 2019. Effects of salinity, pH and growth phase on the protein productivity by Dunaliella salina. Journal of Chemical Technology and Biotechnology 94:1032–40.
   Web of Science ®Google Scholar
• Sulaiman, N., D. I. Givens, and S. Anitha. 2021. A narrative review: In-vitro methods for assessing bio-accessibility/bioavailability of iron in plant-based foods. Frontiers in Sustainable Food Systems 5:392.
   Google Scholar
• Tavano, O. L. 2013. Protein hydrolysis using proteases: An important tool for food biotechnology. Journal of Molecular Catalysis B: Enzymatic 90:1–11.
   Web of Science ®Google Scholar
• Tavano, O. L., V. A. Neves, and S. d. SilvaJr. 2016. In vitro versus in vivo protein digestibility techniques for calculating PDCAAS (protein digestibility-corrected amino acid score) applied to chickpea fractions. Food Research International (Ottawa, ON) 89 (Pt 1):756–63. doi: 10.1016/j.foodres.2016.10.005.
   PubMedGoogle Scholar
• Teuling, E., J. W. Schrama, H. Gruppen, and P. A. Wierenga. 2017a. Effect of cell wall characteristics on algae nutrient digestibility in Nile tilapia (Oreochromis niloticus) and African catfish (Clarus gariepinus). Aquaculture 479:490–500.
   Web of Science ®Google Scholar
• Teuling, E., P. A. Wierenga, J. O. Agboola, H. Gruppen, and J. W. Schrama. 2019. Cell wall disruption increases bioavailability of Nannochloropsis gaditana nutrients for juvenile Nile tilapia (Oreochromis niloticus). Aquaculture 499:269–82.
   Web of Science ®Google Scholar
• Teuling, E., P. A. Wierenga, J. W. Schrama, and H. Gruppen. 2017b. Comparison of protein extracts from various unicellular green sources. Journal of Agricultural and Food Chemistry 65:7989–8002.
   PubMed Web of Science ®Google Scholar
• Thirumdas, R., M. Brnčić, S. R. Brnčić, F. J. Barba, F. Gálvez, S. Zamuz, R. Lacomba, and J. M. Lorenzo. 2018. Evaluating the impact of vegetal and microalgae protein sources on proximate composition, amino acid profile, and physicochemical properties of fermented Spanish “chorizo” sausages. Journal of Food Processing and Preservation 42:e13817.
   Web of Science ®Google Scholar
• Tibbetts, S. M., and S. J. J. Patelakis. 2022. Apparent digestibility coefficients (ADCs) of intact-cell marine microalgae meal (Pavlova sp. 459) for juvenile Atlantic salmon (Salmo salar L.). Aquaculture 546:737236.
   Web of Science ®Google Scholar
• Tibbetts, S. M., C. G. Whitney, M. J. MacPherson, S. Bhatti, A. H. Banskota, R. Stefanova, and P. J. McGinn. 2015. Biochemical characterization of microalgal biomass from freshwater species isolated in Alberta, Canada for animal feed applications. Algal Research 11:435–47.
   Google Scholar
• Tibbetts, S. M., F. Yasumaru, and D. Lemos. 2017. In vitro prediction of digestible protein content of marine microalgae (Nannochloropsis granulata) meals for Pacific white shrimp (Litopenaeus vannamei) and rainbow trout (Oncorhynchus mykiss). Algal Research 21:76–80.
   Google Scholar
• Tibbetts, S. M., J. E. Milley, and S. P. Lall. 2015. Chemical composition and nutritional properties of freshwater and marine microalgal biomass cultured in photobioreactors. Journal of Applied Phycology 27:1109–19.
   Web of Science ®Google Scholar
• Tibbetts, S. M., J. Mann, and A. Dumas. 2017. Apparent digestibility of nutrients, energy, essential amino acids and fatty acids of juvenile Atlantic salmon (Salmo salar L.). Diets containing whole-cell or cell-ruptured Chlorella vulgaris meals at five dietary inclusion levels. Aquaculture 481:25–39.
   Web of Science ®Google Scholar
• Tibbetts, S. M., S. J. J. Patelakis, C. G. Whitney-Lalonde, L. L. Garrison, C. L. Wall, and S. P. MacQuarrie. 2020. Nutrient composition and protein quality of microalgae meals produced from the marine prymnesiophyte Pavlova sp. 459 mass-cultivated in enclosed photobioreactors for potential use in salmonid aquafeeds. Journal of Applied Phycology 32:299–318.
   Web of Science ®Google Scholar
• Tibbetts, S. M., T. MacPherson, P. J. McGinn, and A. H. Fredeen. 2016. In vitro digestion of microalgal biomass from freshwater species isolated in Alberta, Canada for monogastric and ruminant animal feed applications. Algal Research 19:324–32.
   Google Scholar
• United Nations. 2015. Accessed January 27, 2022. https://sdgs.un.org/2030agenda.
   Google Scholar
• Ursu, A. V., A. Marcati, T. Sayd, V. Sante-Lhoutellier, G. Djelveh, and P. Michaud. 2014. Extraction, fractionation and functional properties of proteins from the microalgae Chlorella vulgaris. Bioresource Technology 157:134–9. doi: 10.1016/j.biortech.2014.01.071.
   PubMed Web of Science ®Google Scholar
• van Eck, N. J., and L. Waltman. 2010. Software survey: VOSviewer, a computer program for bibliometric mapping. Scientometrics 84:523–38. doi: 10.1007/s11192-009-0146-3.
   PubMed Web of Science ®Google Scholar
• Venkata Subhash, G., N. Chugh, S. Iyer, A. Waghmare, A. S. Musale, R. Nandru, R. B. Dixit, M. S. Gaikwad, D. Menon, and R. Thorat. 2020. Application of in vitro protein solubility for selection of microalgae biomass as protein ingredient in animal and aquafeed. Journal of Applied Phycology 32:3955–70.
   Web of Science ®Google Scholar
• Verspreet, J., L. Soetemans, C. Gargan, M. Hayes, and L. Bastiaens. 2021. Nutritional profiling and preliminary bioactivity screening of five micro-algae strains cultivated in Northwest Europe. Foods 10:1516.
   PubMed Web of Science ®Google Scholar
• Vizcaíno, A. J., M. I. Sáez, T. F. Martínez, F. G. Acién, and F. J. Alarcón. 2018. Differential hydrolysis of proteins of four microalgae by the digestive enzymes of gilthead sea bream and Senegalese sole. Algal Research 37:145–53.
   Google Scholar
• Waghmare, A. G., M. K. Salve, J. G. LeBlanc, and S. S. Arya. 2016. Concentration and characterization of microalgae proteins from Chlorella pyrenoidosa. Bioresources and Bioprocessing 3:16.
   Google Scholar
• Wang, C., H. Wang, Z. Zhao, S. Xiao, Y. Zhao, C. Duan, L. Gao, S. Li, and J. Wang. 2019. Pediococcus acidilactici AS185 attenuates early atherosclerosis development through inhibition of lipid regulation and inflammation in rats. Journal of Functional Foods 60:103424.
   Web of Science ®Google Scholar
• Wang, S. K., C. Guo, W. Wu, K. Y. Sui, and C. Z. Liu. 2019. Effects of incident light intensity and light path length on cell growth and oil accumulation in Botryococcus braunii (Chlorophyta). Engineering in Life Sciences 19:104–11.
   PubMed Web of Science ®Google Scholar
• Wang, Y., S. M. Tibbetts, F. Berrue, P. J. McGinn, S. P. MacQuarrie, A. Puttaswamy, S. Patelakis, D. Schmidt, R. Melanson, and S. E. MacKenzie. 2020. A rat study to evaluate the protein quality of three green microalgal species and the impact of mechanical cell wall disruption. Foods 9:1531.
   PubMed Web of Science ®Google Scholar
• We Akeson, M. S. 1964. A pepsin-pancreatin digest index of protein quality evaluation. Journal of Nutrition 83:257–61.
   PubMed Web of Science ®Google Scholar
• Weber, S., P. M. Grande, L. M. Blank, and H. Klose. 2022. Insights into cell wall disintegration of Chlorella vulgaris. PLoS One 17:e0262500.
   PubMed Web of Science ®Google Scholar
• Wells, M. L., P. Potin, J. S. Craigie, J. A. Raven, S. S. Merchant, K. E. Helliwell, A. G. Smith, M. E. Camire, and S. H. Brawley. 2017. Algae as nutritional and functional food sources: Revisiting our understanding. Journal of Applied Phycology 29:949–82.
   PubMed Web of Science ®Google Scholar
• Wild, K. J., H. Steingaß, and M. Rodehutscord. 2018. Variability in nutrient composition and in vitro crude protein digestibility of 16 microalgae products. Journal of Animal Physiology and Animal Nutrition 102 (5):1306–19. doi: 10.1111/jpn.12953.
   PubMed Web of Science ®Google Scholar
• Wild, K. J., H. Steingaß, and M. Rodehutscord. 2019. Variability of in vitro ruminal fermentation and nutritional value of cell-disrupted and nondisrupted microalgae for ruminants. GCB Bioenergy 11:345–59.
   Google Scholar
• Xing, G., X. Rui, D. Wang, M. Liu, X. Chen, and M. Dong. 2017. Effect of fermentation pH on protein bioaccessibility of soymilk curd with added tea polyphenols as assessed by in vitro gastrointestinal digestion. Journal of Agricultural and Food Chemistry 65:11125–32.
   PubMed Web of Science ®Google Scholar