Streptococcus pneumoniae Protein Vaccine Candidates: Properties, Activities and Animal Studies

REFERENCES

• Adamou J. E., Heinrichs J. H., Erwin A. L., Walsh W., Gayle T., Dormitzer M., Dagan R., Brewah Y. A., Barren P., Lathigra R., Langermann S., Koenig S., Johnson S. Identification and characterization of a novel family of pneumococcal proteins that are protective against sepsis. Infect. Immun. 2001; 69: 949–958, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Advisory Committee on Immunization Practices. Preventing pneumococcal disease among infants and young children Morbid. Mortal. Weekly Rep. 2000; 49 No RR09: 1–55, [CSA]
   Google Scholar
• Advisory Committee on Immunization Practices. Pneumococcal polysaccharide vaccine. Morbid. Mortal. Weekly Rep. 1989; 38: 64–76, [CSA]
   Google Scholar
• Alexander J. E., Lock R. A., Peeters C. A.M., Poolman J. T., Andrew P. W., Mitchell T. J., Hansman D., Paton J. C. Immunization of mice with pneumolysin toxoid confers a significant degree of protection against at least nine serotypes of Streptococcus pneumoniae. Infect. Immun. 1994; 62: 5683–5688, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Arulanandam B. P., Lynch J. M., Briles D. E., Hollingshead S., Metzger D. W. Intranasal vaccination with pneumococcal surface protein A and interleukin-12 augments antibody-mediated opsonization and protective immunity against Streptococcus pneumoniae Infection. Infect. Immun. 2001; 69: 6718–6724, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Balachandran P., Brooks-Walter A., Virolainen-Julkunen A., Hollingshead S. K., Briles D. E. Role of pneumococcal surface protein C in naso-pharyngeal carriage and pneumonia and its ability to elicit protection against carriage of Streptococcus pneumoniae. Infect. Immun. 2002; 70: 2526–2534, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Balachandran P., Hollingshead S. K., Paton J. C., Briles D. E. The autolytic enzyme LytA of Streptococcus pneumoniae is not responsible for releasing pneumolysin. J. Bacteriol. 2001; 183: 3108–3116, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Beall B., Gherardi G., Facklam R. R., Hollingshead S. K. Pneumococcal pspA sequence types of prevalent multiresistant pneumococ-cal strains in the United States and of internationally disseminated clones. J. Clin. Microbiol. 2000; 38: 3663–3669, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Berry A. M., Lock R. A., Hansman D., Paton J. C. Contribution of autolysin to virulence of Streptococcus pneumoniae. Infect. Immun. 1989; 57: 2324–2330, [INFOTRIEVE], [CSA]
   Google Scholar
• Berry A. M., Paton J. C. Additive attenuation of virulence of Streptococcus pneumoniae by mutation of the genes encoding pneumolysin and other putative pneumococcal virulence proteins. Infect. Immun. 2000; 68: 133–140, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Berry A. M., Paton J. C. Sequence heterogeneity of PsaA, a 37-kilodalton putative adhesin essential for virulence of Streptococcus pneumoniae. Infect. Immun. 1996; 64: 5255–5262, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Boulnois G. J., Paton J. C., Mitchell T. J., Andrew P. W. Structure and function of pneumolysin, the multifunctional, thiol-activated toxin of Streptococcus pneumoniae. Mol. Microbiol. 1991; 5: 2611–2616, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Brandileone M. C., Andrade A. L., Teles E. M., Zanella R. C., Yara T. I., Di Fabio J. L., Hollingshead S. K. Typing of pneumococcal surface protein A (PspA) in Streptococcus pneumoniae isolated during epidemiological surveillance in Brazil: Towards novel pneumococcal protein vaccines. Vaccine. 2004; 22: 3890–3896, [INFOTRIEVE], [CSA], [CROSSREF]
   Google Scholar
• Briles D. E., Ades E., Paton J. C., Sampson J. S., Carlone G. M., Huebner R. C., Virolainen A., Swiatlo E., Hollingshead S. K. Intranasal immunization of mice with a mixture of the pneumococcal proteins PsaA and PspA is highly protective against nasopharyngeal carriage of Streptococcus pneumoniae. Infect. Immun. 2000a; 68: 796–800, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Briles D. E., Hollingshead S., Brooks-Walter A., Nabors G. S., Ferguson L., Schilling M., Gravenstein S., Braun P., King J., Swift A. The potential to use PspA and other pneumococcal proteins to elicit protection against pneumococcal infection. Vaccine 2000b; 18: 1707–1711, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Briles D. E., Hollingshead S. K., King J., Swift A., Braun P. A., Park M. K., Ferguson L. M., Nahm M. H., Nabors G. S. Immunization of humans with recombinant pneumococcal surface protein A (rPspA) elicits antibodies that passively protect mice from fatal infection with Streptococcus pneumoniae bearing heterologous PspA. J. Infect. Dis. 2000c; 182: 1694–1701, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Briles D. E., Hollingshead S. K., Nabors G. S., Paton J. C., Brooks-Walter A. The potential for using protein vaccines to protect against otitis media caused by Streptococcus pneumoniae. Vaccine. 2000d; 19 (Suppl 1): S87–S95, [CSA], [CROSSREF]
   Google Scholar
• Briles D. E., Hollingshead S. K., Paton J. C., Ades E. W., Novak L., van Ginkel F. W., Benjamin Jr. W. H. Immunizations with pneumococcal surface protein A and pneumolysin are protective against pneumonia in a murine model of pulmonary infection with Streptococcus pneumoniae. J. Infect. Dis. 2003; 188: 339–348, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Briles D. E., King J. D., Gray M. A., McDaniel L. S., Swiatlo E., Benton K. A. PspA, a protection-eliciting pneumonoccal protein: Immunogenicity of isolated native PspA in mice. Vaccine. 1996; 14: 858–867, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Briles D. E., Tart R. C., Swiatlo E., Dillard J. P., Smith P., Benton K. A., Ralph B. A., Brooks-Walter A., Crain M. J., Hollingshead S. K., McDaniel L. S. Pneumococcal diversity: Considerations for new vaccine strategies with emphasis on pneumococcal surface protein A (Pspa). Clin. Mircobiol. Rev. 1998; 11: 645–657, [CSA]
   PubMed Web of Science ®Google Scholar
• Brooks-Walter A., Briles D. E., Hollingshead S. K. The pspC gene of Streptococcus pneumoniae encodes a polymorphic protein, PspC, which elicits cross-reactive antibodies to PspA and provides immunity to pneumococcal bacteremia. Infect. Immun. 1999; 67: 6533–6542, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Brown J. S., Gilliand S. M., Holden D. W. A Streptococcus pneumoniae pathogenecity island encoding an ABC transporter involved in iron uptake and virulence. Mol. Microbiol. 2001a; 40: 572–585, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Brown J. S., Gilliland S. M., Ruiz-Albert J., Holden D. W. Characterization of pit, a Streptococcus pneumoniae iron uptake ABC transporter. Infect. Immun. 2002; 70: 4389–4398, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMedGoogle Scholar
• Brown J. S., Ogunniyi A. D., Woodrow M. C., Holden D. W., Paton J. C. Immunization with components of two iron uptake ABC transporters protects mice against systemic Streptococcus pneumoniae infection. Infect. Immun. 2001b; 69: 6702–6706, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Camara M., Boulnois G. J., Andrew P. W., Mitchell T. J. A neuraminidase from Streptococcus pneumoniae has the features of a surface protein. Infect. Immun. 1994; 62: 3688–3695, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Camara M., Mitchell T. J., Andrew P. W., Boulnois G. J. Streptococcus pneumoniae produces at least two distinct enzymes with neuraminidase activity: Cloning and expression of a second neuraminidase gene in Escherichia coli. Infect. Immun. 1991; 59: 2856–2858, [INFOTRIEVE], [CSA]
   Google Scholar
• Cheng Q., Finkel D., Hostetter M. K. Novel purification scheme and functions for a C3-binding protein from Streptococcus pneumoniae. Biochemistry 2000; 39: 5450–5457, [INFOTRIEVE], [CSA], [CROSSREF]
   Google Scholar
• Cima-Cabal M. D., Vazquez F., de, Jr. T. I., Mendez F. J. Rapid and reliable identification of Streptococcus pneumoniae isolates by pneumolysin-mediated agglutination. J. Clin. Microbiol. 1999; 37: 1964–1966, [INFOTRIEVE], [CSA]
   PubMedGoogle Scholar
• Cundell D. R., Weiser J. N., Shen J., Young A., Tuomanen E. I. Relationship between colonial morphology and adherence of Streptococcus pneumoniae. Infect. Immun. 1995; 63: 757–761, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Dagan R., Kayhty H., Wuorimaa T., Yaich M., Bailleux F., Zamir O., Eskola J. Tolerability and immunogenicity of an eleven valent mixed carrier Streptococcus pneumoniae capsular polysaccharide–diphtheria toxoid or tetanus protein conjugate vaccine in Finnish and Israeli infants. Pediatr. Infect. Dis. J. 2004; 23: 91–98, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Dave S., Brooks-Walter A., Pangburn M. K., McDaniel L. S. PspC, a pneumococcal surface protein, binds human factor H. Infect. Immun. 2001; 69: 3435–3437, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Dave S., Pangburn M. K., Pruitt C., McDaniel L. S. Interaction of human factor H with PspC of Streptococcus pneumoniae. Indian J. Med. Res. 2004; 119: Suppl: 66–73, [INFOTRIEVE], [CSA]
   Google Scholar
• de los Toyos J. R., Memdez F. J., Aparocio J. F., Vazquez F., del Mar Garcia Suarez M., Feleites A., Hardisson C., Morgan P. J., Andrew P. W., Mitchell T. J. Functional analysis of pneumolysin by use of monoclonal antibodies. Infect. Immun. 1996; 64: 480–484, [INFOTRIEVE], [CSA]
   Google Scholar
• Dintilhac A., Alloing G., Granadel C., Claverys J. P. Competence and virulence of Streptococcus pneumoniae: Adc and PsaA mutants exhibit a requirement for Zn and Mn resulting from inactivation of putative ABC metal permeases. Mol. Microbiol. 1997; 25: 727–739, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Elm C., Rohde M., Vaerman J. P., Chhatwal G. S., Hammerschmidt S. Characterization of the interaction of the pneumococcal surface protein SpsA with the human polymeric immunoglobulin receptor (hpIgR). Indian J. Med. Res. 2004; 119: Suppl: 61–65, [INFOTRIEVE], [CSA]
   Google Scholar
• Feldman C., Munro N. C., Jeffery P. K., Andrew P. W., Boulnois G. J., Guerreiro D., Rhode J. A.L., Todd H. C., Cole P. J., Wilson R. Pneumolysin induces the salient histologic features of pneumococcal infection in the rat lung in vivo. Amer. J. Respir. Cell Mol. Biol. 1991; 5: 416–423, [CSA]
   PubMed Web of Science ®Google Scholar
• Gor D. O., Ding X., Li Q., Schreiber J. R., Dubinsky M., Greenspan N. S. Enhanced immunogenicity of pneumococcal surface adhesin A by genetic fusion to cytokines and evaluation of protective immunity in mice. Infect. Immun. 2002; 70: 5589–5595, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMedGoogle Scholar
• Green B. A., Zhang Y., Masi A. W., Barniak V., Wetherell M., Smith R. P., Reddy M. S., Zhu D. PppA, a surface-exposed protein of Streptococcus pneumoniae, elicits cross-reactive antibodies that reduce colonization in a murine intranasal immunization and challenge model. Infect. Immun. 2005; 73: 981–989, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Hamel J., Charland N., Pineau I., Ouellet C., Rioux S., Martin D., Brodeur B. R. Prevention of pneumococcal disease in mice immunized with conserved surface-accessible proteins. Infect. Immun. 2004; 72: 2659–2670, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Hammerschmidt S., Talay S. R., Brandtzaeg P., Chhatwal G. S. SpsA, a novel pneumococcal surface protein with specific binding to secretory immunoglobulin A and secretory component. Mol. Microbiol. 1997; 25: 1113–1124, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Hammerschmidt S., Tillig M. P., Wolff S., Vaerman J. P., Chhatwal G. S. Species-specific binding of human secretory component to SpsA protein of Streptococcus pneumoniae via a hexapeptide motif. Mol. Microbiol. 2000; 36: 726–736, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Iannelli F., Oggioni M. R., Pozzi G. Allelic variation in the highly polymorphic locus pspC of Streptococcus pneumoniae. Gene. 2002; 284: 63–71, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Jacobs T., Cima-Cabal M. D., Darji A., Mendez F. J., Vazquez F., Jacobs A. A., Shimada Y., Ohno-Iwashita Y., Weiss S., de I. T., Jr. The conserved undecapeptide shared by thiol-activated cytolysins is involved in membrane binding. FEBS Lett. 1999; 459: 463–466, [INFOTRIEVE], [CSA], [CROSSREF]
   Google Scholar
• Janulczyk R., Iannelli F., Sjoholm A. G., Pozzi G., Bjorck L. Hic, a novel surface protein of Streptococcus pneumoniae that interferes with complement function. J. Biol. Chem. 2000; 275: 37257–37263, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Jedrzejas M. J. Pneumococcal virulence factors: Structure and function. Microbiol. Mol. Biol Rev. 2001; 65: 187–207, [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Johnston J. W., Myers L. E., Ochs M. M., Benjamin Jr. W. H., Briles D. E., Hollingshead S. K. Lipoprotein PsaA in virulence of Streptococcus pneumoniae: Surface accessibility and role in protection from superoxide. Infect. Immun. 2004; 72: 5858–5867, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Jomaa M., Yuste J., Paton J. C., Jones C., Dougan G., Brown J. S. Antibodies to the iron uptake ABC transporter lipoproteins PiaA and PiuA promote opsonophagocytosis of Streptococcus pneumoniae. Infect. Immun. 2005; 73: 6852–6859, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Kaijalainen T., Rintamaki S., Herva E., Leinonen M. Evaluation of gene–technological and conventional methods in the identification of Streptococcus pneumoniae. J. Microbiol. Methods 2002; 51: 111–118, [INFOTRIEVE], [CSA], [CROSSREF]
   Google Scholar
• Kaplan S. L., Mason Jr. E. O., Wald E. R., Schutze G. E., Bradley J. S., Tan T. Q., Hoffman J. A., Givner L. B., Yogev R., Barson W. J. Decrease of invasive pneumococcal infections in children among 8 children's hospitals in the United States after the introduction of the 7-valent pneumococcal conjugate vaccine. Pediatrics. 2004; 113: 443–449, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Kim J. O., Weiser J. N. Association of intrastrain phase variation in quantity of capsular polysaccharide and teichoic acid with the virulence of Streptococcus pneumoniae. J. Infect. Dis. 1998; 177: 368–377, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• King S. J., Whatmore A. M., Dowson C. G. NanA, a neuraminidase from Streptococcus pneumoniae, shows high levels of sequence diversity, at least in part through recombination with Streptococcus oralis. J. Bacteriol. 2005; 187: 5376–5386, [INFOTRIEVE], [CSA]
   Google Scholar
• Kuo J., Douglas M., Ree H. K., Lindberg A. A. Characterization of a recombinant pneumolysin and its use as a protein carrier for pneumococcal type 18C conjugated vaccines. Infect. Immun. 1995; 63: 2706–2713, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Lawrence M. C., Pilling P. A., Epa V. C., Berry A. M., Ogunniyi A. D., Paton J. C. The crystal structure of pneumococcal surface antigen PsaA reveals a metal–binding site and a novel structure for a putative ABC-type binding protein. Structure. 1998; 6: 1553–1561, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Lock R. A., Hansman D., Paton J. C. Comparative efficacy of autolysin and pneumolysin as immunogens protecting mice against infection by Streptococcus pneumoniae. Microb. Pathog. 1992; 12: 137–143, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Lock R. A., Paton J. C., Hansman D. Comparative efficacy of pneumococcal neuraminidase and pneumolysin as immunogens protective against Streptococcus pneumoniae. Microb. Pathog. 1988; 5: 461–467, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Long J. P., Tong H. H., DeMaria T. F. Immunization with native or recombinant Streptococcus pneumoniae neuraminidase affords protection in the chinchilla otitis media model. Infect. Immun. 2004; 72: 4309–4313, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Mbelle N., Huebner R. E., Wasas A. D., Kimura A., Chang I., Klugman K. P. Immunogenicity and impact on nasopharyngeal carriage of a nonavalent pneumococcal conjugate vaccine. J. Infect. Dis. 1999; 180: 1171–1176, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• McDaniel L. S., McDaniel D. O., Hollingshead S. K., Briles D. E. Comparison of the PspA sequence from Streptococcus pneumoniae EF5668 to the previously identifie PspA sequence from strain Rx1 and ability of PspA from EF5668 to elicit protection against pneumococci of different capsular types. Infect. Immun. 1998; 66: 4748–4754, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• McDaniel L. S., Ralph B. A., McDaniel D. O., Briles D. E. Localization of protection-eliciting epitopes on PspA of Streptococcus pneumoniae between amino acid residues 192 and 260. Microb. Pathog. 1994; 17: 323–337, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• McDaniel L. S., Scott G., Kearney J. F., Briles D. E. Monoclonal antibodies against protease-sensitive pneumococcal antigens can protect mice from fatal infection with Streptococcus pneumoniae. J. Exp. Med. 1984; 160: 386–397, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMedGoogle Scholar
• McDaniel L. S., Scott G., Widenhofer K., Carroll J. M., Briles D. E. Analysis of a surface protein of Steeptococcus pneumoniae recognised by protective monoclonal antibodies. Microb. Pathog. 1986; 1: 519–531, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• McDaniel L. S., Sheffield J. S., Delucchi P., Briles D. E. PspA, a surface protein of Streptococcus pneumoniae, is capable of eliciting protection against pneumococci of more than one capsular type. Infect. Immun. 1991; 59: 222–228, [INFOTRIEVE], [CSA]
   PubMedGoogle Scholar
• Michon F., Fusco P. C., Minetti C. A., Laude-Sharp M., Uitz C., Huang C. H., D'Ambra A. J., Moore S., Remeta D. P., Heron I., Blake M. S. Multivalent pneumococcal capsular polysaccharide conjugate vaccines employing genetically detoxified pneumolysin as a carrier protein. Vaccine. 1998; 16: 1732–1741, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Mitchell T. J., Andrew P. W., Saunders F. K., Smith A. N., Boulnois G. J. Complement activation and antibody binding by pneumolysin via a region of the toxin homologous to a human acute-phase protein. Mol. Microbiol. 1991; 5: 1883–1888, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Mollerach M., Regueira M., Bonofiglio L., Callejo R., Pace J., Di Fabio J. L., Hollingshead S., Briles D. Invasive Streptococcus pneumoniae isolates from Argentinian children: Serotypes, families of pneumococcal surface protein A (PspA) and genetic diversity. Epidemiol. Infect. 2004; 132: 177–184, [INFOTRIEVE], [CSA], [CROSSREF]
   Google Scholar
• Navarre W. W., Schneewind O. Proteolytic cleavage and cell wall anchoring at the LPXTG motif of surface proteins in gram-positive bacteria. Mol. Microbiol. 1994; 14: 115–121, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Navarre W. W., Schneewind O. Surface proteins of gram–positive bacteria and mechanisms of their targeting to the cell wall envelope. Microbiol. Mol. Biol. Rev. 1999; 63: 174–229, [CSA]
   PubMed Web of Science ®Google Scholar
• Novak R., Braun J. S., Charpentier E., Tuimanen E. Penicillin tolerance genes of Streptococcus pneumoniae: The ABC-type manganese permease complex PsaA. Mol. Microbiol. 1998; 29: 1285–1296, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMedGoogle Scholar
• O'Brien K. L., Santosham M. Potential impact of conjugate pneumococcal vaccines on pediatric pneumococcal diseases. Am. J. Epidemiol. 2004; 159: 634–644, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMedGoogle Scholar
• Ogunniyi A. D., Folland R. L., Briles D. E., Hollingshead S. K., Paton J. C. Immunization of mice with combinations of pneumococcal virulence proteins elicits enhanced protection against challenge with Streptococcus pneumoniae. Infect. Immun. 2000; 68: 3028–3033, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Ogunniyi A. D., Woodrow M. C., Poolman J. T., Paton J. C. Protection against Streptococcus pneumoniae elicited by immunization with pneumolysin and CbpA. Infect. Immun. 2001; 69: 5997–6003, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Orihuela C. J., Gao G., Francis K. P., Yu J., Tuomanen E. I. Tissue-specific contributions of pneumococcal virulence factors to pathogenesis. J. Infect. Dis. 2004; 190: 1661–1669, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Paton J. C., Ferrante A. Inhibition of human polymorphonuclear leukocyte respiratory burst, bactericidal activity, and migration by pneumolysin. Infect. Immun. 1983; 41: 1212–1216, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Paton J. C., Lock R. A., Lee C. J., Li J. P., Berry A. M., Mitchell T. J., Andrew P. W., Hansman D., Boulnois G. J. Purification and immunogenicity of genetically obtained pneumolysin toxoids and their conjugation to Streptococcus pneumoniae type 19F polysaccharide. Infect. Immun. 1991; 59: 2297–2304, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Pilling P. A., Lawrence M. C., Berry A. M., Ogunniyi A. D., Lock R. A., Paton J. C. Expression, purification and preliminary X-ray crystallographic analysis of PsaA, a putative metal–transporter protein of Streptococcus pneumoniae. Acta Crystallogr. D. Biol. Crystallogr. 1998; 54: 1464–1466, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMedGoogle Scholar
• Rapola S., Kilpi T., Lahdenkari M., Makela P. H., Kayhty H. Antibody response to the pneumococcal proteins pneumococcal surface adhesin A and pneumolysin in children with acute otitis media. Pediatr. Infect. Dis. J. 2001a; 20: 482–487, [CSA]
   Google Scholar
• Rapola S., Kilpi T., Lahdenkari M., Takala A. K., Makela P. H., Kayhty H. Do antibodies to pneumococcal surface adhesin A prevent pneumococcal involvement in acute otitis media?. J. Infect. Dis. 2001b; 184: 577–581, [INFOTRIEVE], [CSA], [CROSSREF]
   Google Scholar
• Ren B., Szalai A. J., Hollingshead S. K., Briles D. E. Effects of PspA and antibodies to PspA on activation and deposition of complement on the pneumococcal surface. Infect. Immun. 2004; 72: 114–122, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Roche H., Hakansson A., Hollingshead S. K., Briles D. E. Regions of PspA/EF3296 best able to elicit protection against Streptococcus pneumoniae in a murine infection model. Infect. Immun. 2003; 71: 1033–1041, [INFOTRIEVE], [CSA], [CROSSREF]
   Google Scholar
• Romero-Steiner S., Libutti D., Pais L. B., Dykes J., Anderson P., Whitin J. C., Keyserling H. L., Carlone G. M. Standardization of an opsonophagocytic assay for the measurement of functional antibidy activity against Streptococcus pneumoniae using differentiated HL-60 cells. Clin. Diagn. Lab. Immunol. 1997; 4: 415–422, [INFOTRIEVE], [CSA]
   PubMedGoogle Scholar
• Romero-Steiner S., Pilishvili T., Sampson J. S., Johnson S. E., Stinson A., Carlone G. M., Ades E. W. Inhibition of pneumococcal adherence to human nasopharyngeal epithelial cells by anti-PsaA antibodies. Clin. Diagn. Lab Immunol. 2003; 10: 246–251, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMedGoogle Scholar
• Rosenow C., Ryan P., Weiser J. N., Johnson S., Fontan P., Ortqvist A., Masure H. R. Contribution of novel choline-binding proteins to adherence, colonization and immunogenicity of Streptococcus pneumoniae. Mol. Microbiol. 1997; 25: 819–829, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Rossjohn J., Gilbert R. J., Crane D., Morgan P. J., Mitchell T. J., Rowe A. J., Andrew P. W., Paton J. C., Tweten R. K., Parker M. W. The molecular mechanism of pneumolysin, a virulence factor from Streptococcus pneumoniae. J. Mol. Biol. 1998; 284: 449–461, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Russell H., Tharpe J. A., Wells D. E., White E. H., Johnson J. E. Monoclonal antibody recognizing a species-specific protein from Streptococcus pneumoniae. J. Clin. Microbiol. 1990; 28: 2191–2195, [INFOTRIEVE], [CSA]
   PubMedGoogle Scholar
• Sampson J. S., O'Connor S. P., Stinson A. R., Tharpe J. A., Russell H. Cloning and nucleotide sequence analysis of psaA the Streptococcus pneumoniae gene encoding a 37-kilodalton protein homologous to previously reported Streptococcus sp. adhesins. Infect. Immun. 1994; 62: 319–324, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Saukkoriipi A., Palmu A., Kilpi T., Leinonen M. Real-time quantitative PCR for the detection of Streptococcus pneumoniae in the middle ear fluid of children with acute otitis media. Mol. Cell Probes. 2002; 16: 385–390, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Saunders F. K., Mitchell T. J., Walker J. A., Andrew P. W., Boulnois G. J. Pneumolysin, the thio–activated toxin of Streptococcus pneumoniae, does not require a thio group for in vitro activity. Infect. Immun. 1989; 57: 2547–2552, [INFOTRIEVE], [CSA]
   Google Scholar
• Schneewind O., Mihaylova–Petkov D., Model P. Cell wall sorting signals in surface proteins of gram–positive bacteria. EMBO J. 1993; 12: 4803–4811, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Seo J. Y., Seong S. Y., Ahn B. Y., Kwon I. C., Chung H., Jeong S. Y. Cross-protective immunity of mice induced by oral immunization with pneumococcal surface adhesin A encapsulated in microspheres. Infect. Immun. 2002; 70: 1143–1149, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Shaper M., Hollingshead S. K., Benjamin W. H., Jr., Briles D. E. PspA protects Streptococcus pneumoniae from killing by apolactoferrin, and antibody to PspA enhances killing of Pneumococci by apolactoferrin. Infect. Immun. 2004; 72: 5031–5040, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Sheppard C. L., Harrison T. G., Morris R., Hogan A., George R. C. Autolysin-targeted LightCycler assay including internal process control for detection of Streptococcus pneumoniae DNA in clinical samples. J. Med. Microbiol. 2004; 53: 189–195, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Simell B., Korkeila M., Pursiainen H., Kilpi T. M., Kayhty H. Pneumococcal carriage and otitis media induce salivary antibodies to pneumococcal surface adhesin A, pneumolysin, and pneumococcal surface protein a in children. J. Infect. Dis. 2001; 183: 887–896, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMedGoogle Scholar
• Smart L. E., Dougall A. J., Girdward R. W.A. New 23-valent pneumococcal vaccine in relation to pneumococcal serotypes in systemic and systemic disease. J. Infect. 1987; 14: 209–215, [INFOTRIEVE], [CSA], [CROSSREF]
   Google Scholar
• Steinfort C., Wilson R., Rutman A., Sykes D., Todd H., Walker J., Mitchell T., Saunders K., Andrew P., Boulnois G., Cole P. Pneumolysin produced by Streptococcus pneumoniae damages human respiratory epithelium in vitro. Chest 1989; 95 Suppl: 221S, [CSA]
   Google Scholar
• Tai S. S., Lee C.-J., Winter R. E. Hemin utilization is related to virulence of Streptococcus pneumoniae. Infect. Immun. 1993; 61: 5401–5405, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Tai S. S., Yu C., Lee J. K. A solute binding protein of Streptococcus pneumoniae iron transport. FEMS Microbiol. Lett. 2003; 220: 303–308, [INFOTRIEVE], [CSA], [CROSSREF]
   Google Scholar
• Talkington D. F., Brown B. G., Tharpe J. T., Koenig A., Russell H. Protection of mice against fatal pneumococcal challenge by immunization with pneumococcal surface adhesin (PsaA). Microb. Pathog. 1996; 21: 17–22, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Talkington D. F., Crimmins D. L., Voellinger D. C., Yother J., Briles D. E. A 43-kilodalton pneumococcal surface protein, PspA: Isolation, protective abilities, and structural analysis of the amino-terminal sequence. Infect. Immun. 1991; 59: 1285–1289, [INFOTRIEVE], [CSA]
   Google Scholar
• Tart R. C., McDaniel L. S., Ralph B. A., Briles D. E. Truncated Streptococcus pneumoniae PspA molecules elicit cross–protective immunity against pneumococcal challenge in mice. J. Infect. Dis. 1996; 173: 380–386, [INFOTRIEVE], [CSA]
   PubMedGoogle Scholar
• Tong H. H., Blue L. E., James M. A., DeMaria T. F. Evaluation of the virulence of a Streptococcus pneumoniae neuraminidase- deficient mutant in nasopharyngeal colonization and development of otitis media in the chinchilla model. Infect. Immun. 2000; 68: 921–924, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Tong H. H., James M., Grants I., Liu X., Shi G., DeMaria T. F. Comparison of structural changes of cell surface carbohydrates in the eustachian tube epithelium of chinchillas infected with a Streptococcus pneumoniae neuraminidase-deficient mutant or its isogenic parent strain. Microb. Pathog. 2001; 31: 309–317, [INFOTRIEVE], [CSA], [CROSSREF]
   Google Scholar
• Tong H. H., McIver M. A., Fisher L. M., DeMaria T. F. Effect of lacto-N-neotetraose, asialoganglioside-GM1 and neuraminidase on adherence of otitis media-associated serotypes of Streptococcus pneumoniae to chinchilla tracheal epithelium. Microb. Pathog. 1999; 26: 111–119, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Usobiaga P., Medrano F. J., Gasset M., Garcia J. L., Saiz J. L., Rivas G., Laynez J., Menendez M. Structural organization of the major autolysin from Streptococcus pneumoniae. J. Biol. Chem. 1996; 271: 6832–6838, [INFOTRIEVE], [CSA]
   Google Scholar
• Vela Coral M. C., Fonseca N., Castaneda E., Di Fabio J. L., Hollingshead S. K., Briles D. E. Pneumococcal surface protein A of invasive Streptococcus pneumoniae isolates from Colombian children. Emerg. Infect. Dis. 2001; 7: 832–836, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Weiser J. N., Austrian R., Sreenivasan P. K., Masure H. R. Phase variation in pneumococcal opacity: Relationship between colonial morphology and nasopharyngeal colonization. Infect. Immun. 1994; 62: 2582–2589, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Wizemann T. M., Heinrichs J. H., Adamou J. E., Erwin A. L., Kunsch C., Choi G. H., Barash S. C., Rosen C. A., Masure H. R., Toumanen E., Gayle A., Brewah Y. A., Walsh W., Barren P., Lathigra R., Hanson M., Langermann S., Johnson S., Kogoma T. Use of a whole genome approach to identify vaccine molecules affording protection against Streptococcus pneumoniae infection. Infect. Immun. 2001; 69: 1593–1598, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Wortham C., Grinberg L., Kaslow D. C., Briles D. E., McDaniel L. S., Lees A., Flora M., Snapper C. M., Mond J. J. Enhanced protective antibody responses to PspA after intranasal or subcutaneous injections of PspA genetically fused to granulocyte-macrophage colony-stimulating factor or interleukin-2. Infect. Immun. 1998; 66: 1513–1520, [INFOTRIEVE], [CSA]
   Google Scholar
• Yamamoto M., McDaniel L. S., Kawabata K., Briles D. E., Jackson R., McGhee J. R., Kiyono H. Oral immunization with PspA elicits protective humoral immunity against Streptococcus pneumoniae infection. Infect. Immun. 1997; 65: 640–644, [INFOTRIEVE], [CSA]
   Web of Science ®Google Scholar
• Yother J., Briles D. E. Structural properties and evolutionay relationships of PspA, a surface protein of Streptococcus pneumoniae, as revealed by sequence analysis. J. Bacteriol. 1992; 174: 601–609, [INFOTRIEVE], [CSA]
   Google Scholar
• Yother J., White J. M. Novel surface attachment mechanism of the Streptococcus pneumoniae protein PspA. J. Bacteriol. 1994; 176: 2976–2985, [INFOTRIEVE], [CSA]
   PubMed Web of Science ®Google Scholar
• Zhang J. R., Mostov K. E., Lamm M. E., Nanno M., Shimida S., Ohwaki M., Tuomanen E. The polymeric immunoglobulin receptor translocates pneumococci across human nasopharyngeal epithelial cells. Cell. 2000; 102: 827–837, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar
• Zhang Y., Masi A. W., Barniak V., Mountzouros K., Hostetter M. K., Green B. A. Recombinant PhpA protein, a unique histidine motif-containing protein from Streptococcus pneumoniae, protects mice against intranasal pneumococcal challenge. Infect. Immun. 2001; 69: 3827–3836, [INFOTRIEVE], [CSA], [CROSSREF]
   PubMed Web of Science ®Google Scholar